Photosynthesis Research最新文献_第4页

From leaf to multiscale models of photosynthesis: applications and challenges for crop improvement 从叶片到多尺度光合作用模型：作物改良的应用与挑战

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-04-15 DOI: 10.1007/s11120-024-01083-9

Alexandrina Stirbet, Ya Guo, Dušan Lazár, Govindjee Govindjee

{"title":"From leaf to multiscale models of photosynthesis: applications and challenges for crop improvement","authors":"Alexandrina Stirbet, Ya Guo, Dušan Lazár, Govindjee Govindjee","doi":"10.1007/s11120-024-01083-9","DOIUrl":"https://doi.org/10.1007/s11120-024-01083-9","url":null,"abstract":"To keep up with the growth of human population and to circumvent deleterious effects of global climate change, it is essential to enhance crop yield to achieve higher production. Here we review mathematical models of oxygenic photosynthesis that are extensively used, and discuss in depth a subset that accounts for diverse approaches providing solutions to our objective. These include models (1) to study different ways to enhance photosynthesis, such as fine-tuning antenna size, photoprotection and electron transport; (2) to bioengineer carbon metabolism; and (3) to evaluate the interactions between the process of photosynthesis and the seasonal crop dynamics, or those that have included statistical whole-genome prediction methods to quantify the impact of photosynthesis traits on the improvement of crop yield. We conclude by emphasizing that the results obtained in these studies clearly demonstrate that mathematical modelling is a key tool to examine different approaches to improve photosynthesis for better productivity, while effective multiscale crop models, especially those that also include remote sensing data, are indispensable to verify different strategies to obtain maximized crop yields.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140584021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling large charge transfer character of PSII in an iron-deficient cyanobacterial membrane: A Stark fluorescence spectroscopy study 揭示缺铁蓝藻膜中 PSII 的大电荷转移特性：斯塔克荧光光谱研究

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-04-15 DOI: 10.1007/s11120-024-01099-1

Anjue Mane Ara, Sandrine D’Haene, Rienk van Grondelle, Md. Wahadoszamen

{"title":"Unveiling large charge transfer character of PSII in an iron-deficient cyanobacterial membrane: A Stark fluorescence spectroscopy study","authors":"Anjue Mane Ara, Sandrine D’Haene, Rienk van Grondelle, Md. Wahadoszamen","doi":"10.1007/s11120-024-01099-1","DOIUrl":"https://doi.org/10.1007/s11120-024-01099-1","url":null,"abstract":"In this work, we applied Stark fluorescence spectroscopy to an iron-stressed cyanobacterial membrane to reveal key insights about the electronic structures and excited state dynamics of the two important pigment-protein complexes, IsiA and PSII, both of which prevail simultaneously within the membrane during iron deficiency and whose fluorescence spectra are highly overlapped and hence often hardly resolved by conventional fluorescence spectroscopy. Thanks to the ability of Stark fluorescence spectroscopy, the fluorescence signatures of the two complexes could be plausibly recognized and disentangled. The systematic analysis of the SF spectra, carried out by employing standard Liptay formalism with a realistic spectral deconvolution protocol, revealed that the IsiA in an intact membrane retains almost identical excited state electronic structures and dynamics as compared to the isolated IsiA we reported in our earlier study. Moreover, the analysis uncovered that the excited state of the PSII subunit of the intact membrane possesses a significantly large CT character. The observed notably large magnitude of the excited state CT character may signify the supplementary role of PSII in regulative energy dissipation during iron deficiency.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140584268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ApcE plays an important role in light-induced excitation energy dissipation in the Synechocystis PCC6803 phycobilisomes. ApcE 在 Synechocystis PCC6803 植物体的光诱导激发能量耗散中发挥着重要作用。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-04-01 Epub Date: 2024-02-26 DOI: 10.1007/s11120-024-01078-6

Gonfa Tesfaye Assefa, Joshua L Botha, Bertus van Heerden, Farooq Kyeyune, Tjaart P J Krüger, Michal Gwizdala

{"title":"ApcE plays an important role in light-induced excitation energy dissipation in the Synechocystis PCC6803 phycobilisomes.","authors":"Gonfa Tesfaye Assefa, Joshua L Botha, Bertus van Heerden, Farooq Kyeyune, Tjaart P J Krüger, Michal Gwizdala","doi":"10.1007/s11120-024-01078-6","DOIUrl":"10.1007/s11120-024-01078-6","url":null,"abstract":"Phycobilisomes (PBs) play an important role in cyanobacterial photosynthesis. They capture light and transfer excitation energy to the photosynthetic reaction centres. PBs are also central to some photoprotective and photoregulatory mechanisms that help sustain photosynthesis under non-optimal conditions. Amongst the mechanisms involved in excitation energy dissipation that are activated in response to excessive illumination is a recently discovered light-induced mechanism that is intrinsic to PBs and has been the least studied. Here, we used single-molecule spectroscopy and developed robust data analysis methods to explore the role of a terminal emitter subunit, ApcE, in this intrinsic, light-induced mechanism. We isolated the PBs from WT Synechocystis PCC 6803 as well as from the ApcE-C190S mutant of this strain and compared the dynamics of their fluorescence emission. PBs isolated from the mutant (i.e., ApcE-C190S-PBs), despite not binding some of the red-shifted pigments in the complex, showed similar global emission dynamics to WT-PBs. However, a detailed analysis of dynamics in the core revealed that the ApcE-C190S-PBs are less likely than WT-PBs to enter quenched states under illumination but still fully capable of doing so. This result points to an important but not exclusive role of the ApcE pigments in the light-induced intrinsic excitation energy dissipation mechanism in PBs.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11006782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Pelletier and Caventou (1817, 1818) papers on chlorophyll and beyond. 关于叶绿素及其他的 Pelletier 和 Caventou（1817 年和 1818 年）论文。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-04-01 Epub Date: 2024-03-15 DOI: 10.1007/s11120-024-01081-x

Govindjee Govindjee, Alexandrina Stirbet, Jonathan S Lindsey, Hugo Scheer

引用次数: 0

Modulating spectral response of raw photosynthetic pigments via ternary cadmium chalcogenide quantum dots: simultaneous enhancement at green spectrum and inhibition at UV region. 通过三元铬化镉量子点调节原始光合色素的光谱响应：同时增强绿色光谱和抑制紫外线区域。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-04-01 Epub Date: 2024-02-26 DOI: 10.1007/s11120-024-01085-7

Sümeyye Aykut, Nida Ük, İbrahim Yağız Coşkun, Sultan Şahin Keskin, Ilgın Nar, Levent Trabzon, Caner Ünlü

{"title":"Modulating spectral response of raw photosynthetic pigments via ternary cadmium chalcogenide quantum dots: simultaneous enhancement at green spectrum and inhibition at UV region.","authors":"Sümeyye Aykut, Nida Ük, İbrahim Yağız Coşkun, Sultan Şahin Keskin, Ilgın Nar, Levent Trabzon, Caner Ünlü","doi":"10.1007/s11120-024-01085-7","DOIUrl":"10.1007/s11120-024-01085-7","url":null,"abstract":"Photosynthesis relies on the absorption of sunlight by photosynthetic pigments (PPs) such as chlorophylls and carotenoids. While these pigments are outstanding at harvesting light, their natural structure restricts their ability to harvest light at specific wavelengths. In this study, Oleic acid-capped CdSeS and CdTeS ternary quantum dots (QDs) were synthesized using a novel two-phase synthesis method. Then, these QDs were used to interact with raw PPs, a mixture of chlorophylls and carotenoids isolated from spinach. Our findings revealed the following: (1) Interacting QDs with raw PPs effectively inhibited the chlorophyll fluorescence of the pigments upon excitation in UV light region (250-400 nm) without causing any damage to their structure. (2) By forming an interaction with QDs, the chlorophyll fluorescence of raw PPs could be induced through excitation with green-light spectrum. (3) The composition of the QDs played a fundamental role in their interaction with PPs. Our study demonstrated that the photophysical properties of isolated PPs could be modified by using cadmium-based QDs by preserving the structure of the pigments themselves.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11006769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139973060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro demetalation of central magnesium in various chlorophyll derivatives using Mg-dechelatase homolog from the chloroflexi Anaerolineae. 利用叶绿素植物 Anaerolineae 中的镁脱螯酶同源物对各种叶绿素衍生物中的中心镁进行体外脱金属。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-04-01 Epub Date: 2024-03-26 DOI: 10.1007/s11120-024-01088-4

Soma Sato, Mitsuaki Hirose, Ryouichi Tanaka, Hisashi Ito, Hitoshi Tamiaki

{"title":"In vitro demetalation of central magnesium in various chlorophyll derivatives using Mg-dechelatase homolog from the chloroflexi Anaerolineae.","authors":"Soma Sato, Mitsuaki Hirose, Ryouichi Tanaka, Hisashi Ito, Hitoshi Tamiaki","doi":"10.1007/s11120-024-01088-4","DOIUrl":"10.1007/s11120-024-01088-4","url":null,"abstract":"In the metabolic pathway of chlorophylls (Chls), an enzyme called STAY-GREEN or SGR catalyzes the removal of the central magnesium ion of Chls and their derivatives to their corresponding free bases, including pheophytins. The substrate specificity of SGR has been investigated through in vitro reactions using Chl-related molecules. However, information about the biochemical properties and reaction mechanisms of SGR and its substrate specificity remains elusive. In this study, we synthesized various Chl derivatives and investigated their in vitro dechelations using an SGR enzyme. Chl-a derivatives with the C3-vinyl group on the A-ring, which is commonly found as a substituent in natural substrates, and their analogs with ethyl, hydroxymethyl, formyl, and styryl groups at the C3-position were prepared as substrates. In vitro dechelatase reactions of these substrates were performed using an SGR enzyme derived from an Anaerolineae bacterium, allowing us to investigate their specificity. Reactivity was reduced for substrates with an electron-withdrawing formyl or sterically demanding styryl group at the C3-position. Furthermore, the Chl derivative with the C8-styryl group on the B-ring was less reactive for SGR dechelation than the C3-styryl substrate. These results indicate that the SGR enzyme recognizes substituents on the B-ring of substrates more than those on the A-ring.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11006732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140294237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the simulation and interpretation of substrate-water exchange experiments in photosynthetic water oxidation. 关于光合作用水氧化过程中基质-水交换实验的模拟和解释。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-03-21 DOI: 10.1007/s11120-024-01084-8

Petko Chernev, A Orkun Aydin, Johannes Messinger

{"title":"On the simulation and interpretation of substrate-water exchange experiments in photosynthetic water oxidation.","authors":"Petko Chernev, A Orkun Aydin, Johannes Messinger","doi":"10.1007/s11120-024-01084-8","DOIUrl":"https://doi.org/10.1007/s11120-024-01084-8","url":null,"abstract":"Water oxidation by photosystem II (PSII) sustains most life on Earth, but the molecular mechanism of this unique process remains controversial. The ongoing identification of the binding sites and modes of the two water-derived substrate oxygens ('substrate waters') in the various intermediates (Si states, i = 0, 1, 2, 3, 4) that the water-splitting tetra-manganese calcium penta-oxygen (Mn4CaO5) cluster attains during the reaction cycle provides central information towards resolving the unique chemistry of biological water oxidation. Mass spectrometric measurements of single- and double-labeled dioxygen species after various incubation times of PSII with H218O provide insight into the substrate binding modes and sites via determination of exchange rates. Such experiments have revealed that the two substrate waters exchange with different rates that vary independently with the Si state and are hence referred to as the fast (Wf) and the slow (WS) substrate waters. New insight for the molecular interpretation of these rates arises from our recent finding that in the S2 state, under special experimental conditions, two different rates of WS exchange are observed that appear to correlate with the high spin and low spin conformations of the Mn4CaO5 cluster. Here, we reexamine and unite various proposed methods for extracting and assigning rate constants from this recent data set. The analysis results in a molecular model for substrate-water binding and exchange that reconciles the expected non-exchangeability of the central oxo bridge O5 when located between two Mn(IV) ions with the experimental and theoretical assignment of O5 as WS in all S states. The analysis also excludes other published proposals for explaining the water exchange kinetics.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Excitation transfer and quenching in photosystem II, enlightened by carotenoid triplet state in leaves 叶片中类胡萝卜素三重态启示下的光系统 II 的激发传递和淬灭

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-03-19 DOI: 10.1007/s11120-024-01086-6

Agu Laisk, Richard B. Peterson, Vello Oja

{"title":"Excitation transfer and quenching in photosystem II, enlightened by carotenoid triplet state in leaves","authors":"Agu Laisk, Richard B. Peterson, Vello Oja","doi":"10.1007/s11120-024-01086-6","DOIUrl":"https://doi.org/10.1007/s11120-024-01086-6","url":null,"abstract":"Accumulation of carotenoid (Car) triplet states was investigated by singlet–triplet annihilation, measured as chlorophyll (Chl) fluorescence quenching in sunflower and lettuce leaves. The leaves were illuminated by Xe flashes of 4 μs length at half-height and 525–565 or 410–490 nm spectral band, maximum intensity 2 mol quanta m−2 s−1, flash photon dose up to 10 μmol m−2 or 4–10 PSII excitations. Superimposed upon the non-photochemically unquenched Fmd state, fluorescence was strongly quenched near the flash maximum (minimum yield Fe), but returned to the Fmd level after 30–50 μs. The fraction of PSII containing a 3Car in equilibrium with singlet excitation was calculated as Te = (Fmd—Fe)/Fmd. Light dependence of Te was a rectangular hyperbola, whose initial slope and plateau were determined by the quantum yields of triplet formation and annihilation and by the triplet lifetime. The intrinsic lifetime was 9 μs, but it was strongly shortened by the presence of O2. The triplet yield was 0.66 without nonphotochemical quenching (NPQ) but approached zero when NP-Quenched fluorescence approached 0.2 Fmd. The results show that in the Fmd state a light-adapted charge-separated PSIIL state is formed (Sipka et al., The Plant Cell 33:1286–1302, 2021) in which Pheo−P680+ radical pair formation is hindered, and excitation is terminated in the antenna by 3Car formation. The results confirm that there is no excitonic connectivity between PSII units. In the PSIIL state each PSII is individually turned into the NPQ state, where excess excitation is quenched in the antenna without 3Car formation.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140166394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

International conference on “Photosynthesis and Hydrogen Energy Research for Sustainability-2023”: in honor of Robert Blankenship, Győző Garab, Michael Grätzel, Norman Hüner and Gunnar Öquist 光合作用和氢能研究促进可持续性-2023 年 "国际会议：纪念 Robert Blankenship、Győző Garab、Michael Grätzel、Norman Hüner 和 Gunnar Öquist

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-03-19 DOI: 10.1007/s11120-024-01087-5

引用次数: 0

Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides. 了解光系统 II 的结晶：不同分子大小的聚乙二醇对烷基麦芽糖苷胶束形成的影响。

IF 3.7 3区生物学

Photosynthesis Research Pub Date : 2024-03-15 DOI: 10.1007/s11120-024-01079-5

Frank Müh, Adrian Bothe, Athina Zouni

{"title":"Towards understanding the crystallization of photosystem II: influence of poly(ethylene glycol) of various molecular sizes on the micelle formation of alkyl maltosides.","authors":"Frank Müh, Adrian Bothe, Athina Zouni","doi":"10.1007/s11120-024-01079-5","DOIUrl":"https://doi.org/10.1007/s11120-024-01079-5","url":null,"abstract":"The influence of poly(ethylene glycol) (PEG) polymers H-(O-CH2-CH2)p-OH with different average molecular sizes <math><mi>p</mi></math> on the micelle formation of n-alkyl-β-D-maltoside detergents with the number of carbon atoms in the alkyl chain ranging from <math><mrow><mn>10</mn></mrow> </math> to <math><mrow><mn>12</mn></mrow> </math> is investigated with the aim to learn more about the detergent behavior under conditions suitable for the crystallization of the photosynthetic pigment-protein complex photosystem II. PEG is shown to increase the critical micelle concentration (CMC) of all three detergents in the crystallization buffer in a way that the free energy of micelle formation increases linearly with the concentration of oxyethylene units (O-CH2-CH2) irrespective of the actual molecular weight of the polymer. The CMC shift is modeled by assuming for simplicity that it is dominated by the interaction between PEG and detergent monomers and is interpreted in terms of an increase of the transfer free energy of a methylene group of the alkyl chain by 0.2 kJ mol-1 per 1 mol L-1 increase of the concentration of oxyethylene units at 298 K. Implications of this effect for the solubilization and crystallization of protein-detergent complexes as well as detergent extraction from crystals are discussed.","PeriodicalId":20130,"journal":{"name":"Photosynthesis Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140137093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0