Physiological Entomology最新文献_第7页

Developmental diet, life stage and thermal acclimation affect thermal tolerance of the fall armyworm, Spodoptera frugiperda 发育日粮、生活期和热驯化对秋粘虫的热耐受性有影响

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-07-26 DOI: 10.1111/phen.12414

Siphephelo M. Phungula, K. Krüger, R. S. Nofemela, C. Weldon

引用次数: 0

Developmental diet, life stage and thermal acclimation affect thermal tolerance of the fall armyworm, Spodoptera frugiperda 发育期饮食、生活阶段和热适应对草地贪夜蛾耐热性的影响

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-07-26 DOI: 10.1111/phen.12414

Siphephelo M. Phungula, Kerstin Krüger, Robert S. Nofemela, Christopher W. Weldon

{"title":"Developmental diet, life stage and thermal acclimation affect thermal tolerance of the fall armyworm, Spodoptera frugiperda","authors":"Siphephelo M. Phungula, Kerstin Krüger, Robert S. Nofemela, Christopher W. Weldon","doi":"10.1111/phen.12414","DOIUrl":"https://doi.org/10.1111/phen.12414","url":null,"abstract":"Insect thermal tolerance affects survival and distribution of species but can vary within and between individuals due to the environmental conditions they experience. The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), recently invaded Africa and its local hotspots are associated with weather and crop phenology. We investigated the effects of larval diet (maize plants, wheat plants or chickpea‐based artificial diet), life stages and acclimation temperature on thermal tolerance traits of FAW to explore how these variables might contribute to its presence in the field. First and sixth instar larvae and adults reared on each diet at 25°C were acclimated for 24 h at 20, 25 or 30°C. We then recorded the critical thermal minimum (CTmin) and critical thermal maximum (CTmax) of individuals. Sixth instars had the highest CTmin but the effects of acclimation and diet on this trait depended on the life stage being tested. CTmin of first instars increased with acclimation temperature when fed on an artificial diet, but sixth instars and adults were not affected by acclimation or larval diet. CTmax was lowest among adults but acclimation and diet again had effects that differed between life stages. CTmax of first instars and adults increased with acclimation temperature but not in sixth instars. Sixth instars and adults reared on the artificial diet had the highest CTmax but diet had no effect on first instar CTmax. Our results show the complexity of thermal tolerance across FAW life stages and suggest the need to consider local temperature variation and available dietary resources when predicting their potential distribution.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 4","pages":"122-131"},"PeriodicalIF":1.5,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71986215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity, domain duplication, birth-and-death and adaptive evolution of fatty acyl-CoA reductase genes in Hemiptera 半翅目脂肪酰基辅酶A还原酶基因的多样性、结构域复制、生灭及适应性进化

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-06-28 DOI: 10.1111/phen.12413

Zhongjuan Di, Jiahui Tian, Muhammad Yasir Ali, Biyun Zhang, Shiyong Yang, Yuejun Fu, Fengqi Li, Chen Luo

{"title":"Diversity, domain duplication, birth-and-death and adaptive evolution of fatty acyl-CoA reductase genes in Hemiptera","authors":"Zhongjuan Di, Jiahui Tian, Muhammad Yasir Ali, Biyun Zhang, Shiyong Yang, Yuejun Fu, Fengqi Li, Chen Luo","doi":"10.1111/phen.12413","DOIUrl":"https://doi.org/10.1111/phen.12413","url":null,"abstract":"Fatty acyl-CoA reductases (FARs) play an important role in the synthesis of fatty alcohols in various organisms. Based on the important function of FAR genes, they can be used as a potential molecular target for controlling agricultural pests. Although the FAR genes have been studied in a number of insects, the gain, loss, and molecular evolution of FAR genes between different Hemipteran species still require comprehensive and systematic study. This study systematically identified and analysed 352 FAR genes from 12 Hemipteran species, including six typical true bug species, Cimex lectularius, Apolygus lucorum, Halyomorpha halys, Oncopeltus fasciatus, Rhodnius prolixus and Gerris buenoi. The number of FAR genes per species ranged from 17 to 43, and a phylogenetic analysis showed that the identified FAR genes of Hemiptera can be classified into 11 clades. The gain and loss of FAR genes have occurred in some Hemipteran species. These FAR genes conform to the birth-and-death model in the evolutionary process. Through selection pressure analysis, we determined that G. buenoi in clade 11 evolved under the pressure of positive selection, with the evolutionary sites of A at position 214 and T at position 451, thus clarifying the differences in amino acids among species and providing a better understanding of the molecular evolutionary mechanism of Hemipteran FAR. In addition, structural analysis of the FAR genes revealed duplication of the two conservative domains, the Rossmann-fold domain and the sterile domain, of the FAR in four species, namely Bemisia tabaci, Diaphorina citri, R. prolixus and Trialeurodes vaporariorum. This study lays a foundation for further studies on the molecular functions of Hemiptera FAR, and provides a possible new target for the control of Hemiptera, especially the stink bugs.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"97-106"},"PeriodicalIF":1.5,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50146711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-evolutionary implications for a possible contribution of cuticle hardening system in insect evolution and terrestrialisation 角质层硬化系统在昆虫进化和地球化中的可能贡献的生态进化意义

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-04-17 DOI: 10.1111/phen.12406

Tsunaki Asano, Kosei Hashimoto, R. Craig Everroad

{"title":"Eco-evolutionary implications for a possible contribution of cuticle hardening system in insect evolution and terrestrialisation","authors":"Tsunaki Asano, Kosei Hashimoto, R. Craig Everroad","doi":"10.1111/phen.12406","DOIUrl":"https://doi.org/10.1111/phen.12406","url":null,"abstract":"Previously we proposed one aspect of how insects could adapt to terrestrial environments during their evolution. The hypothesis is based on a theory that insects have evolved an insect-specific system for cuticle formation mediated by an insect-specific enzyme. This enzyme, multicopper oxidase-2 (MCO2), catalyses the oxidation of catecholamines using molecular oxygen as the acceptor of electrons from the substrates. A potential advantage of this MCO2-mediated system is the utilization of molecular oxygen abundant in the atmosphere, which is different from the case in crustaceans (close relatives of insects) that utilize calcium ions. Accumulation of calcium ions leads to an increase in weight, but the lightweight cuticle without calcification might have been a critical factor enabling insects to evolve flight first in the history of Metazoa. Our theory also provides a simple explanation to a long-standing question of why insects are so rare in marine environments. In previous reviews, we have mainly focused on the details of the biochemical processes occurring in arthropod cuticles, but here only the essence of our theory is extracted and briefly summarized with newly added information.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"55-60"},"PeriodicalIF":1.5,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/phen.12406","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50135472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zinc methionine or zinc sulphate supplementation modulate the development of the hypopharyngeal gland and expression of major royal jelly protein genes in Apis mellifera L. bees 蛋氨酸锌或硫酸锌对蜜蜂下咽腺发育和主要蜂王浆蛋白基因表达的调节作用

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-04-13 DOI: 10.1111/phen.12407

Giovanna do Prado Ribeiro, Samir Moura Kadri, Luis Antônio Justulin, Paulo Eduardo Martins Ribolla, Ricardo de Oliveira Orsi

{"title":"Zinc methionine or zinc sulphate supplementation modulate the development of the hypopharyngeal gland and expression of major royal jelly protein genes in Apis mellifera L. bees","authors":"Giovanna do Prado Ribeiro, Samir Moura Kadri, Luis Antônio Justulin, Paulo Eduardo Martins Ribolla, Ricardo de Oliveira Orsi","doi":"10.1111/phen.12407","DOIUrl":"https://doi.org/10.1111/phen.12407","url":null,"abstract":"We analysed whether the source of zinc (inorganic and organic) influences the morphology of the hypopharyngeal gland (HPG) and the relative expression of major royal jelly protein (MRJP) genes in nursing bees at 6 days of age. For this, 15 colonies of Apis mellifera bees were subjected to the following treatments: Zn0 (control), zinc methionine (50 ppm) and zinc sulphate monohydrate (50 ppm) during the off-season (characterized by food reduction). MRJP1 and MRJP5 gene expression was analysed using real-time PCR and HPG sections under a microscope. Bee mortality and population development were also evaluated for 30 days. It was observed that the honeybees consumed all sugar syrup containing zinc both in its organic and inorganic forms, which did not promote mortality or affect population development. Regarding HPG morphometry, it was observed that zinc, regardless of the source, promoted the acini area significantly greater than that of the control. Zn methionine promoted a significant increase in HPG area compared with that of an inorganic source. The relative expression of MRJP1 and MRJP5 was not significantly affected compared with the control. We conclude that the mineral Zn promotes an increase in the HPG area and does not alter the pattern of expression of the MRJP1 and MRJP5 genes, and that the organic source of the mineral presents the best results.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"90-96"},"PeriodicalIF":1.5,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50139911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrogressive moulting in khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae) ಖಾಪ್ರ ಜೀರುಂಡೆಯಲ್ಲಿ ಹಿಮ್ಮುಖ ಪೊರೆ ಬಿಡುವಿಕೆ 金龟甲的退行性换羽（鞘翅目：Dermestidae）

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-01-25 DOI: 10.1111/phen.12404

Sunil Shivananjappa, Kevin D. Floate, Paul G. Fields, Robert A. Laird

{"title":"Retrogressive moulting in khapra beetle, Trogoderma granarium (Coleoptera: Dermestidae)\u0000 ಖಾಪ್ರ ಜೀರುಂಡೆಯಲ್ಲಿ ಹಿಮ್ಮುಖ ಪೊರೆ ಬಿಡುವಿಕೆ","authors":"Sunil Shivananjappa, Kevin D. Floate, Paul G. Fields, Robert A. Laird","doi":"10.1111/phen.12404","DOIUrl":"https://doi.org/10.1111/phen.12404","url":null,"abstract":"Insect larvae typically moult to grow, but here we investigate insect larvae that moult to shrink; that is, retrogressive moulting or retrogressive development. We demonstrate this phenomenon in khapra beetle, Trogoderma granarium Everts (Dermestidae), among the world's most invasive pests of stored grains and cereal products, and a quarantine pest of interest for many countries. Larvae survived a 3-month period of starvation, moulting up to six times and reducing their body mass by about half, on average. When reprovisioned with food, most larvae resumed the normal trajectory of development and pupated within a month. Thus, retrogressive development is a mechanism that may favour species whose resources exhibit feast-or-famine dynamics. By enabling survival during periods of privation, retrogressive development contributes to the invasiveness of the khapra beetle by allowing them to persist for long periods in empty storage facilities or empty containers used for international grain shipments.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"75-82"},"PeriodicalIF":1.5,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative analysis of wing interference patterns in Drosophila spp. using hyperspectral images 利用高光谱图像对果蝇翅膀干扰模式的定量分析

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-01-18 DOI: 10.1111/phen.12405

Kazuo H. Takahashi

{"title":"Quantitative analysis of wing interference patterns in Drosophila spp. using hyperspectral images","authors":"Kazuo H. Takahashi","doi":"10.1111/phen.12405","DOIUrl":"https://doi.org/10.1111/phen.12405","url":null,"abstract":"Recent studies have reported wing interference patterns (WIPs), which reflect the microstructure of the wing, for small insects belonging to the Diptera and Hymenoptera orders. WIPs have been evaluated using RGB or multispectral images, but in contrast to these approaches, hyperspectral images allow a more detailed analysis of spectral variation, which may not be captured by RGB or multispectral images. Here, I investigated the WIPs of 12 Drosophila species using hyperspectral images. The average spectrum was calculated for each of the six compartments of the wing region and for the entire wing, including all six compartments. This information was used to evaluate sexual and interspecific differences in the WIPs of 12 Drosophila species. In addition, the possibility of species discrimination based on WIPs was explored using the random forest machine learning algorithm. The present study demonstrates significant sex and interspecific differences in WIPs for each of the six compartments of the wing regions as well as for the entire wing region. The results of the random forest machine learning algorithm suggested the possibility of species identification based on WIPs.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"83-89"},"PeriodicalIF":1.5,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50152007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent changes in reproductive allocation in a facultative ectoparasite, the blowfly Lucilia sericata (Meigen) (Calliphoridae) 一种兼性外寄生虫，丝斑蝇（Meigen）（丽蝇科）生殖分配的年龄依赖性变化

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2023-01-05 DOI: 10.1111/phen.12403

Shatha Alqurashi, Sinead English, Richard Wall

{"title":"Age-dependent changes in reproductive allocation in a facultative ectoparasite, the blowfly Lucilia sericata (Meigen) (Calliphoridae)","authors":"Shatha Alqurashi, Sinead English, Richard Wall","doi":"10.1111/phen.12403","DOIUrl":"https://doi.org/10.1111/phen.12403","url":null,"abstract":"Organisms trade-off limited resources between life-history traits to maximize fitness. In particular, costs associated with reproduction are balanced against somatic maintenance and this can result in age-dependent changes in the optimal allocation of resource to reproduction. Changes in the allocation of resources to reproduction with age were considered in the facultatively parasitic blowfly Lucilia sericata (Diptera: Calliphoridae), using biochemical analysis of lipids in the body and ovary, and lipid and protein in individual eggs. Resource allocation to reproduction, measured as lipid content in the ovary, declined over time. This decline was associated with the production of fewer and smaller eggs per batch. The lipid content of the residual body did not change. A decrease in lipid and increase in protein contents of individual eggs over time, although statistically significant, were relatively slight, suggesting that age-related changes in nutritional allocation to individual eggs were more subtle than changes in egg batch number or size. This study highlights the insights to be gained from considering both biochemical measures of nutritional allocation, and observable measures of reproductive effort, when evaluating how females balance allocation across competing life-history traits. Future work should explore how allocation patterns might vary under conditions of resource constraint and whether age-dependent allocation in laboratory flies is representative of that found in wild populations.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"68-74"},"PeriodicalIF":1.5,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/phen.12403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50130382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The peritrophic matrix delays Nosema ceranae infection in the honey bee Apis mellifera midgut 环营养基质延缓蜜蜂中肠内角鼻线虫感染

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2022-12-05 DOI: 10.1111/phen.12402

André Henrique de Oliveira, Amanda Martins da Cruz Souza, Matheus Tudor Cândido Santos de Resende, Lenise Silva Carneiro, Jacineumo Falcão de Oliveira, Raissa Santana Serra, José Eduardo Serrão

{"title":"The peritrophic matrix delays Nosema ceranae infection in the honey bee Apis mellifera midgut","authors":"André Henrique de Oliveira, Amanda Martins da Cruz Souza, Matheus Tudor Cândido Santos de Resende, Lenise Silva Carneiro, Jacineumo Falcão de Oliveira, Raissa Santana Serra, José Eduardo Serrão","doi":"10.1111/phen.12402","DOIUrl":"https://doi.org/10.1111/phen.12402","url":null,"abstract":"The midgut of bees plays a significant role in digestion and absorption and is a gateway for pathogens. As a protection for the organism, the midgut lumen is lined by a peritrophic matrix rich in chitin fibrils, associated with proteins that form multiple resistant layers with gelatinous consistency, with several functions, including the mechanical barrier against infection by pathogens. The obligate intracellular microsporid Nosema ceranae is an important pathogen that decreases honey production, increases pesticide susceptibility, and may cause mortality in the honey bee Apis mellifera. This study evaluated whether the peritrophic matrix has a protective function against infection by N. ceranae in A. mellifera adult workers. The peritrophic matrix integrity was changed by feeding the bees with calcofluor white (CFW), followed by an artificial infection with N. ceranae spores. Results shown that 0.5% CFW affect the integrity of the peritrophic matrix with areas of interruption of this envelope. This disorganization of the peritrophic matrix reduces the life cycle period of N. ceranae from eight to four days, after inoculation, and increases the concentration of new spores released in the midgut lumen. This is the first report on the importance of the peritrophic matrix as a protective barrier against N. ceranae infection in A. mellifera.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 2-3","pages":"61-67"},"PeriodicalIF":1.5,"publicationDate":"2022-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50131853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Submergence tolerance in immature stages of the stalk-eyed fly Sphyracephala detrahens (Diptera: Diopsidae) 柄眼蝇成虫未成熟期的耐淹性（直翅目：直翅目）

IF 1.5 4区农林科学

Physiological Entomology Pub Date : 2022-11-28 DOI: 10.1111/phen.12401

Ayumi Kudo

{"title":"Submergence tolerance in immature stages of the stalk-eyed fly Sphyracephala detrahens (Diptera: Diopsidae)","authors":"Ayumi Kudo","doi":"10.1111/phen.12401","DOIUrl":"https://doi.org/10.1111/phen.12401","url":null,"abstract":"Sphyracephala detrahens (Walker, 1860) (Diptera: Diopsidae) inhabits the riparian zones of streams and rivers. Because of the limited dispersal ability of S. detrahens during egg, larval, and pupal stages, immature individuals are at risk of being submerged by floodwater after heavy rain. In this study, I evaluated the submergence tolerances of immatures of S. detrahens by comparing them to immatures of Drosophila melanogaster, which also feed on rotten fruits but are not restricted to the riparian zone. The results showed that S. detrahens eggs were susceptible to desiccation, but more than 80% of eggs hatched after full submergence. Later instar larvae were more resistant to full submergence than earlier instar larvae. The duration of submergence causing 50% pupation (PD50) in the first, second, and third‐instar larvae of S. detrahens were 15.88, 58.46, and 91.74 h, respectively. The PD50 of the third‐instar larvae of D. melanogaster was 20.01 h. Third‐instar S. detrahens larvae continued to develop in water for a longer duration than D. melanogaster larvae of the same instar. In the pupal stages, late pupae tended to remain afloat longer than early pupae. The duration of submergence causing 50% emergence (ED50) of adults from early and late pupae were 40.70 and 104.74 h, respectively. In the larval and pupal stages, individuals in the later developmental phases tended to be more tolerant to full submergence. The submergence tolerance of the immature stages of S. detrahens may reflect adaptation to an environment with fluctuating water levels.","PeriodicalId":20081,"journal":{"name":"Physiological Entomology","volume":"48 1","pages":"45-53"},"PeriodicalIF":1.5,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50155360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0