Effects of neuropeptide F2 on female reproduction in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

IF 1.6 4区 农林科学 Q2 ENTOMOLOGY
Fariba Habibi, Azam Mikani, Mohammad Mehrabadi, Maged Mohamed Ali Fouda
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Abstract

Recently, neuropeptide F2 (NPF2) has been demonstrated to increase food consumption and body weight in the cotton bollworm, Helicoverpa armigera. This study evaluated the effect of NPF2 on the adult female reproductive system of H. armigera. Daily NPF2 injections increased oocyte size, ecdysteroid titre in ovaries and haemolymph and the expression level of ecdysone receptor (EcR), ultraspiracle (USP) and vitellogenin (Vg) genes. However, NPF2 injection had no effect on the expression level of hormone receptor 3 (HR3). Fecundity and fertility were increased after the injection of NPF2. The knockdown of the NPF2 gene using RNA interference (RNAi) had opposite effects compared to NPF2 injection. Overall, these results revealed that NPF2 has a regulatory role in the female reproduction of the cotton bollworm.

Abstract Image

神经肽F2对棉铃虫雌性繁殖的影响(鳞翅目:夜蛾科)
近年来,神经肽F2(NPF2)已被证明能增加棉铃虫的食物消耗和体重。本研究评价了NPF2对棉铃虫成年雌性生殖系统的影响。每天注射NPF2可增加卵母细胞大小、卵巢和血淋巴中的蜕皮甾体滴度,以及蜕皮甾酮受体(EcR)、超螺旋体(USP)和卵黄蛋白原(Vg)基因的表达水平。然而,注射NPF2对激素受体3(HR3)的表达水平没有影响。注射NPF2后,受精率和生育能力都有所提高。与注射NPF2相比,使用RNA干扰(RNAi)敲低NPF2基因具有相反的效果。总之,这些结果表明NPF2在棉铃虫的雌性繁殖中具有调节作用。
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来源期刊
Physiological Entomology
Physiological Entomology 生物-昆虫学
CiteScore
2.80
自引率
6.70%
发文量
21
审稿时长
>12 weeks
期刊介绍: Physiological Entomology broadly considers “how insects work” and how they are adapted to their environments at all levels from genes and molecules, anatomy and structure, to behaviour and interactions of whole organisms. We publish high quality experiment based papers reporting research on insects and other arthropods as well as occasional reviews. The journal thus has a focus on physiological and experimental approaches to understanding how insects function. The broad subject coverage of the Journal includes, but is not limited to: -experimental analysis of behaviour- behavioural physiology and biochemistry- neurobiology and sensory physiology- general physiology- circadian rhythms and photoperiodism- chemical ecology
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