Pakistan journal of pharmaceutical sciences最新文献_第4页

In-silico evaluation of the antibiofilm potential of Anredera cordifolia extract, Syzygium aromaticum, and Cinnamomum burmanii essential oils against Staphyloccocus aureus. cordifolia提取物、Syzygium aromaticum和Cinnamomum burmanii精油对金黄色葡萄球菌的抗菌潜力的计算机评价。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.12170.1

Meta Safitri, Nanik Sulistyani, Iis Wahyuningsih, Zenith Putri Dewianti, Arini Aprilliani

{"title":"In-silico evaluation of the antibiofilm potential of Anredera cordifolia extract, Syzygium aromaticum, and Cinnamomum burmanii essential oils against Staphyloccocus aureus.","authors":"Meta Safitri, Nanik Sulistyani, Iis Wahyuningsih, Zenith Putri Dewianti, Arini Aprilliani","doi":"10.36721/PJPS.2025.38.5.REG.12170.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.12170.1","url":null,"abstract":"The increase of antibiotic resistance has been one of major challenges in today's healthcare. Staphylococcus aureus is responsible for approximately 60-80% of human infections. This research examines the anti-biofilm activity of key components of Binahong leaves (Anredera cordifolia), clove flower essential oil (Syzygium aromaticum) and cinnamon essential oil (Cinnamomum burmanii) against Staphylococcus aureus bacteria using in silico simulations. The compounds of plant secondary metabolites were analyzed using Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS) and Gas Chromatography-Mass Spectrometry (GC-MS) techniques to identify the main compounds. In silico studies employed the molecular docking method with BIOVIA Discovery Study Visualizer 2021 and iGEMDOCK V2.1 applications. The LC-HRMS analysis revealed that the binahong leaf ethanol extract contains vitexin, a flavonoid compound with a Cloud Best Match value of 97.4% and an Area of 657851019.181973. GC-MS analysis demonstrated that clove flower essential oil (Syzygium aromaticum) predominantly consists of eugenol, accounting for 83.89% of the oil, whereas cinnamon essential oil (Cinnamomum burmanii) contains cinnamaldehyde, constituting 72.44%. Through molecular docking analysis, it was observed that the vitexin compounds exhibited the highest potential for anti-biofilm activity. These findings provide valuable insights into the antibiofilm potential of Anredera cordifolia, Syzygium aromaticum, and Cinnamomum burmanii against Staphylococcus aureus.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1547-1557"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical efficacy and safety of repetitive transcranial magnetic stimulation (RTMS) in combination with antipsychotic medications in patients with schizophrenia. 反复经颅磁刺激（RTMS）联合抗精神病药物治疗精神分裂症患者的临床疗效和安全性。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.13925.1

Jiahui Yin, Bojian Dai, Ruru Chen, Lingyi Dai, Lixue Qiu

{"title":"Clinical efficacy and safety of repetitive transcranial magnetic stimulation (RTMS) in combination with antipsychotic medications in patients with schizophrenia.","authors":"Jiahui Yin, Bojian Dai, Ruru Chen, Lingyi Dai, Lixue Qiu","doi":"10.36721/PJPS.2025.38.5.REG.13925.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.13925.1","url":null,"abstract":"The global prevalence of schizophrenia is about 0.40% and in some parts of my country it is 0.54%-0.94%. Its treatment faces many difficulties. Antipsychotic drugs are commonly used treatments, but they have many side effects; repetitive transcranial magnetic stimulation (rTMS), as a non-invasive physical therapy, has a certain effect in improving mental and living abilities. This study used a randomized controlled trial to divide 100 patients into an experimental group (rTMS + drugs) and a control group (drugs only) and evaluated them with multiple scales. The relapse rate during the 24-month follow-up period was compared to evaluate rehabilitation and economic value. The results showed that the experimental group had lower Positive and Negative Syndrome Scale (PANSS), higher efficacy and lower relapse rates after treatment; both groups had improved quality of life and cognitive function-related indicators and the experimental group was more significant; the experimental group had higher Wisconsin Card Sorting Test (WCST) scores and better economic benefits; there were no serious adverse events in both groups. In summary, rTMS combined with antipsychotic drugs has good efficacy and safety in the treatment of schizophrenia, can improve patients' quality of life and economic benefits and provide a better choice for clinical treatment.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1558-1566"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of different doses of transdermal fentanyl on the reproductive system in male rats. 不同剂量芬太尼透皮对雄性大鼠生殖系统的影响。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.14330.1

Volkan Kosal, Nurettin Kurt, Caglar Okulmus, Omer Faruk Keles

{"title":"Effect of different doses of transdermal fentanyl on the reproductive system in male rats.","authors":"Volkan Kosal, Nurettin Kurt, Caglar Okulmus, Omer Faruk Keles","doi":"10.36721/PJPS.2025.38.5.REG.14330.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.14330.1","url":null,"abstract":"Fentanyl, a potent synthetic opioid analgesic, is commonly used to manage severe pain. However, the effects of fentanyl use on male reproductive health have not been adequately studied. This study aimed to investigate the effects of different doses of transdermal fentanyl patches on various reproductive parameters in male rats. Adult male Albino Wistar rats were divided into four groups. The treatment groups received transdermal fentanyl at doses of 25 mcg/h (Group II), 50 mcg/h (Group III), and 100 mcg/h (Group IV) for 9 days, respectively. Sperm motility, sperm concentration, abnormal sperm, live/dead sperm, testicular apoptosis, testicular oxidative stress, and androgen receptor levels were evaluated. The results showed that fentanyl administration decreased the oxidative stress parameters CAT and SOD1 levels in all treatment groups (p<0.001). No significant changes were observed in sperm motility, abnormal sperm ratio, or live/dead sperm ratio. However, Group IV showed a significant increase in sperm concentration compared to the other groups (p<0.001). In addition, all fentanyl treatment groups showed a significant increase in apoptosis-related Caspase 3/8/9 enzymes (p<0.001). This study reveals the effects of fentanyl on male reproductive health. This is the first study to demonstrate an increase in sperm concentration associated with high fentanyl doses.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1694-1699"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the efficacy of vitamin D combined with aspirin and immunoglobulin in treating children with the acute Kawasaki disease. 维生素D联合阿司匹林和免疫球蛋白治疗儿童急性川崎病的疗效评价。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.13960.1

Yuan Cheng, QingFeng Fang, BiQuan Chen

{"title":"Evaluation of the efficacy of vitamin D combined with aspirin and immunoglobulin in treating children with the acute Kawasaki disease.","authors":"Yuan Cheng, QingFeng Fang, BiQuan Chen","doi":"10.36721/PJPS.2025.38.5.REG.13960.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.13960.1","url":null,"abstract":"The acute Kawasaki Disease (KD) is a pediatric condition that can cause significant cardiovascular damage, particularly affecting the coronary arteries. Recent research suggests that vitamin D regulates the immune responses and inflammation, potentially improving outcomes in KD. A randomized control trial involving 120 children aged 1-5 years assigned participants to either a treatment group (receiving intravenous immunoglobulin [IVIG], aspirin, and vitamin D; n=60) or a control group (receiving IVIG and aspirin only; n=60). Clinical symptoms, blood routine indices, and serum inflammatory markers (IL-1β, IL-6, and TNF-α) were assessed before and after treatment. Compared to the control group, the treatment group exhibited significantly faster fever resolution (antipyretic time: 27.2±1.3 hours vs. 50.4±2.4 hours in the control group, p < 0.001), lower incidence of IVIG adverse reactions (19 cases vs. 8 cases in the control group, p = 0.031), and reduced levels of inflammatory markers (WBC, CRP, ESR and platelet count). Additionally, the treatment group had lower post-treatment levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). These findings suggest that vitamin D supplementation may modulate the immune response and improve clinical outcomes in children with KD.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1589-1592"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining Silybum marianum as a natural countermeasure to 1,4-dioxane induced hemato-, hepato- and nephrotoxicity in male Sprague-Dawley rats. 水飞蓟对1,4-二恶烷诱导的雄性sd大鼠血液、肝脏和肾脏毒性的天然对抗作用。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.14421.1

Abdul Rauf, Farhat Jabeen

{"title":"Examining Silybum marianum as a natural countermeasure to 1,4-dioxane induced hemato-, hepato- and nephrotoxicity in male Sprague-Dawley rats.","authors":"Abdul Rauf, Farhat Jabeen","doi":"10.36721/PJPS.2025.38.5.REG.14421.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.14421.1","url":null,"abstract":"Silybum marianum (S. marianum) is famous for its nutritional value and medicinal benefits; while 1,4-dioxane is extensively used at the industrial level and in daily routine, but with all its uses, it is also becoming a major water contaminant with hazardous impacts on human health. The current study assessed the therapeutic potential of the ethanolic extract of S. marianum leaves against 1,4-dioxane induced hemato- hepato- nephrotoxicity in male Sprague-Dawley (SD) rats by involving 40 male SD rats, distributed into eight groups viz., control group (C), S. marianum extract groups (S1, S2 and S3 at 85, 165 and 247 mg/kg, respectively), positive control group (G1): treated with 1,4-dioxane at 3000 ppm, and three co-treated groups (G2, G3, G4: 1,4-dioxane at 3000 ppm + S. marianum at 85, 165 and 247 mg/kg, respectively). After the completion of the trial (60 days), significant (P<0.05) improvements in body weight, hepatic-, renal- and lipid profile as well as histo-architecture of liver and kidney were observed in co-treated groups in a dose-dependent manner. While 1,4-dioxane at 3000 ppm severely altered the selected parameters in SD rats. Conclusively, S. marianum, due to its therapeutic potential at 247 mg/kg, countermeasured the 1,4-dioxane induced hemato-, hepato- and nephrotoxicity in male SD rats.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1765-1774"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progesterone suppresses estrogen receptor-mediated inflammatory pathways following intracerebral hemorrhage. 黄体酮抑制脑出血后雌激素受体介导的炎症途径。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.13201.1

Hengyang Ouyang, Xiaobing Zhou, Zhiming Zhang, Lingfeng Lai

{"title":"Progesterone suppresses estrogen receptor-mediated inflammatory pathways following intracerebral hemorrhage.","authors":"Hengyang Ouyang, Xiaobing Zhou, Zhiming Zhang, Lingfeng Lai","doi":"10.36721/PJPS.2025.38.5.REG.13201.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.13201.1","url":null,"abstract":"Intracerebral hemorrhage (ICH) is a highly fatal neurological disease with few successful treatments. The aim of the current study was to investigate the neuroprotection by progesterone and the related molecular mechanisms following ICH. Mice were treated with progesterone (8 mg/kg), estrogen receptor (ER) agonist-erteberel (10 nmol/2 μL), or ER-β-specific siRNA (si-ER-β, 6 nmol/2 μL). Neurological function, edema in the brain and inflammatory cytokine levels were tested. Progesterone significantly increased neurological function on day 1 to day 7 post-ICH and reduced cerebral water content compared to the control group on day 7. Progesterone also suppressed estrogen receptor beta (ER-β) and decreased inflammatory mediator levels such as prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) in ICH-evoked brain tissues and in LPS-stimulated BV-2 microglial cells. These anti-inflammatory effects were inhibited by erteberel, indicating direct interaction with ER-β signaling. Furthermore, progesterone treatment inhibited the expression of Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) p65 via inhibition of ER-β. In summary, our findings show that progesterone is neuroprotective after ICH by modulating the ER-β/TLR4/NF-κB pathway and suggest its therapeutic value for managing post-ICH inflammation.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1722-1730"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oleuropein attenuated docetaxel-induced liver and kidney toxicity in rats by modulating oxidative stress, gene expressions and histopathological damage. 橄榄苦苷通过调节氧化应激、基因表达和组织病理学损伤，减轻多西紫杉醇诱导的大鼠肝肾毒性。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.12955.1

Metin Deniz Karakoc, Ozlem Ozmen, Munevver Nazlıcan Zengin, Osman Ciftci

{"title":"Oleuropein attenuated docetaxel-induced liver and kidney toxicity in rats by modulating oxidative stress, gene expressions and histopathological damage.","authors":"Metin Deniz Karakoc, Ozlem Ozmen, Munevver Nazlıcan Zengin, Osman Ciftci","doi":"10.36721/PJPS.2025.38.5.REG.12955.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.12955.1","url":null,"abstract":"Docetaxel (Dtx) is a frequently used antineoplastic agent despite its dose-limiting toxic effects. Our objective was to assess the effects of oleuropein (Ole), a natural polyphenol, on Dtx-induced toxicity. Thirty-two male rats were randomly assigned to four groups for a four-week treatment: Control (sham), Dtx (5 mg/kg weekly, i.p.), Ole (30 mg/kg daily, p.o.) and Dtx+Ole. Biochemical and gene expression analyses were performed on liver, kidney and blood samples. Additionally, histological and immunohistochemical evaluations were conducted on the liver and kidneys. Ole reduced the Dtx-induced oxidative stress index in tissues. In contrast to Dtx, it decreased caspase-3 and Bax gene expressions while increasing Bcl-2 expression. Furthermore, Ole improved the ALT, AST, urea and creatinine levels, which were impaired by Dtx administration. It also reduced serum IL-6, IL-1β and TNF-α levels, which had been elevated due to Dtx. Histopathological and immunohistochemical examinations revealed that Ole administration mitigated Dtx-related damage in both tissues. These findings suggest that Ole might offer protection against Dtx-induced liver and kidney toxicity in rats.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1993-2005"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145293133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cantharidic acid inhibits the malignant progression of colorectal cancer by inhibiting aerobic glycolysis and regulating the PI3K/Akt/P53 pathway. 斑蝥素酸通过抑制有氧糖酵解和调节PI3K/Akt/P53通路抑制结直肠癌的恶性进展。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.14346.1

Yan Wei, Shulin Dai, Dongyun Zhang, Ting Zhang, Xiaoyu Wang, Bolin Liu, Wei Huang, Yin Li, Mingliao Niu

{"title":"Cantharidic acid inhibits the malignant progression of colorectal cancer by inhibiting aerobic glycolysis and regulating the PI3K/Akt/P53 pathway.","authors":"Yan Wei, Shulin Dai, Dongyun Zhang, Ting Zhang, Xiaoyu Wang, Bolin Liu, Wei Huang, Yin Li, Mingliao Niu","doi":"10.36721/PJPS.2025.38.5.REG.14346.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.14346.1","url":null,"abstract":"Cantharidic acid (CA) has shown effective anticancer activity against many solid tumor cells, but it has not been reported in colorectal cancer (CRC). The PFKFB3 overexpression vector was transfected into SW480 and HT29 cells and the cells were treated with CA and PI3K activator 740 Y-P for 24 h. The malignant progression of the cells was evaluated through CCK-8, EdU, Transwell, flow cytometry, LDH release assay and Hoechst 33258 fluorescence. The expressions of aerobic glycolysis (AEG) and PI3K/Akt/P53 pathway were detected using the kit, extracellular acidification rate (ECAR) assay and Western blot. The subcutaneous tumor model was established by subcutaneous injection of SW480 cells. CA could significantly reduce the proliferation, migration and invasion of SW480 and HT29 cells and promote apoptosis and trigger cell cycle arrest. CA could reduce glucose uptake, lactic acid production and glycolytic capacity, reduce p-PI3K and p-Akt protein levels, raise P53 protein level. PFKFB3 overexpressed promoted CRC malignant progression. 740 Y-P could increase the AEG of CRC cells. Finally, CA reduced the volume and weight of CRC xenografts in mice and inhibited AEG and malignant biological behavior. In conclusion, CA inhibited AEG and malignant progression of CRC cells by regulating the PI3K/Akt/P53 pathway.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1632-1646"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the anti-dyslipidemic activity of Pandanus amaryllifollus leaf extract in diabetic rat model: A combined in-vivo and molecular docking approach. 揭示香兜草叶提取物对糖尿病大鼠模型的抗血脂异常作用：体内与分子相结合的对接方法。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.13879.1

Sonia Khan, Mahwish Akhtar, Noor Jahan, Syed Mohsin Turab, Sana Shamim, Muhammad Imran, Syeda Afroz

{"title":"Unraveling the anti-dyslipidemic activity of Pandanus amaryllifollus leaf extract in diabetic rat model: A combined in-vivo and molecular docking approach.","authors":"Sonia Khan, Mahwish Akhtar, Noor Jahan, Syed Mohsin Turab, Sana Shamim, Muhammad Imran, Syeda Afroz","doi":"10.36721/PJPS.2025.38.5.REG.13879.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.13879.1","url":null,"abstract":"The plant Pandanus amaryllifolius Roxb (pandan), has been shown to have antidyslipidemic activity. This study showed the activity of various alkaloids and flavonoids from pandanus leaf that worked as antidyslipidemic. The in-vivo testing was done by taking the blood samples of normal and diabetic male albino rat and assessed the lipid profile. The molecular docking testing was done by the interactions of the alkaloids and flavonoids with the enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, Peroxisome Proliferator Activator Receptor (PPAR) alpha and the Niemann Pick C1 Like 1 receptor (NPC1L1) at receptor 1HW9, 6LX4 and 7DFZ respectively. Analyses were carried out by studying the binding affinity of the different alkaloids (pandanamine, pandamarilactonine A and pandamarilactonine B) and flavonoids (catechin, epicatechin, epigallocatechin, kaemferol, myricetin, quercetin) present in pandan leaves. Cholestrol HDL ratio, triglycerides, VLDL decreased and HDL increased. The binding energy of alkaloids pandamarilactonine B at 6LX4 was -9.3 kcal/mol, at 7DFZ were -8.9 and at 1HW9 receptor binding energy of pandamarilactonine A was more negative as compared to standard drug simvastatin showed higher level of interaction between the above mentioned alkaloid, flavonoids and receptors. The studied concluded that pandanus alkaloids and flavonoids have marked good antidyslipidemic activity.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1940-1951"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145293139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis, characterization and antidiabetic effects of zinc oxide nanoparticles synthesized using aqueous extract of Cydonia graveolens. 枸杞水提物合成氧化锌纳米颗粒的绿色合成、表征及降糖作用。

IF 0.7 4区医学

Pakistan journal of pharmaceutical sciences Pub Date : 2025-09-01 DOI: 10.36721/PJPS.2025.38.5.REG.13884.1

Muhammad Sajid Khan, Sabira Sultana, Muhammad Akram

{"title":"Green synthesis, characterization and antidiabetic effects of zinc oxide nanoparticles synthesized using aqueous extract of Cydonia graveolens.","authors":"Muhammad Sajid Khan, Sabira Sultana, Muhammad Akram","doi":"10.36721/PJPS.2025.38.5.REG.13884.1","DOIUrl":"https://doi.org/10.36721/PJPS.2025.38.5.REG.13884.1","url":null,"abstract":"This study focused on the green synthesis of zinc oxide nanoparticles (ZnO-NPs) using the aqueous extract of C. graveolens and evaluated their antidiabetic activity. The extract served as both a reducing and capping agent. Synthesized ZnO-NPs were characterized by FTIR, XRD, SEM, and Zetasizer to determine their structural, morphological, and optical properties. Characterization confirmed the successful formation of spherical, crystalline ZnO-NPs with sizes ranging from 20-50 nm. FTIR spectra indicated the role of hydroxyl and carbonyl groups in nanoparticle stabilization. The antidiabetic activity of the ZnO-NPs was assessed through in vitro alpha-glucosidase and alpha-amylase inhibition assays. A concentration-dependent increase in alpha-glucosidase inhibition was observed, with inhibition rates of 67.8% at 50 µg/mL and 86.9% at 100 µg/mL. Similarly, alpha-amylase inhibition reached 81.7% at 100 µg/mL. These findings suggest that the enhanced activity may be due to the synergistic effects of zinc ions and phytochemicals from the plant extract. Overall, green-synthesized ZnO-NPs from C. graveolens demonstrate significant in vitro antidiabetic potential via dual enzyme inhibition. Further in vivo and clinical studies are recommended to confirm their therapeutic efficacy and safety, positioning them as a natural and cost-effective approach for managing type 2 diabetes.","PeriodicalId":19971,"journal":{"name":"Pakistan journal of pharmaceutical sciences","volume":"38 5","pages":"1647-1655"},"PeriodicalIF":0.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0