Pathology最新文献_第2页

Artificial intelligence automation of urine culture reading with BD Kiestra Urine Culture Application: measurement of performance and potential efficiency gains. 人工智能自动化尿液培养阅读与BD Kiestra尿液培养应用：测量性能和潜在的效率收益。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-19 DOI: 10.1016/j.pathol.2025.02.011

Maryza Graham, Leanne Tilson, Tony M Korman, Denise Liow, Harshini Wickremasinghe, Richard Streitberg, John Hamblin

{"title":"Artificial intelligence automation of urine culture reading with BD Kiestra Urine Culture Application: measurement of performance and potential efficiency gains.","authors":"Maryza Graham, Leanne Tilson, Tony M Korman, Denise Liow, Harshini Wickremasinghe, Richard Streitberg, John Hamblin","doi":"10.1016/j.pathol.2025.02.011","DOIUrl":"https://doi.org/10.1016/j.pathol.2025.02.011","url":null,"abstract":"Automation of microbiology sample inoculation and incubation has recently been shown to improve microbiological and clinical endpoints (such as turnaround time) as well as improve efficiency. However, instruments for automation of microbiology sample culture reading and interpretation have only recently become available for clinical evaluation. We evaluated the BD Kiestra Urine Culture Application (UCA) for culture interpretation of clinical urine samples and compared the results to the reading of Kiestra image by scientific staff. Of the 1021 urine samples processed by both UCA and scientist culture reading, 1003 (98%) yielded concordant results at 18-h incubation. The number of samples with ≥104 colony-forming units (CFU)/mL (≥107 CFU/L) which were correctly recognised by the UCA using the early growth detection algorithm was 424/424 (100%). We found the UCA to be an accurate artificial intelligence solution, and we describe the potential for large workload efficiency gains in addition to more rapid report turnaround times.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in coeliac disease serology practices may impact biopsy-free diagnosis in Australasia. 乳糜泻血清学实践的差异可能会影响澳大利亚的无活检诊断。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-18 DOI: 10.1016/j.pathol.2025.02.012

Christie Glennan, Kristie Chapman, Louise Wienholt, Jason A Tye-Din

引用次数: 0

Dual-reporting aldosterone by mass spectrometry and immunoassay in the seated saline suppression test may enhance subtype classification of patients with primary aldosteronism. 质谱法和免疫测定法在生理盐水抑制试验中双重报告醛固酮可能增强原发性醛固酮增多症患者的亚型分类。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-18 DOI: 10.1016/j.pathol.2025.02.008

Nicola Sawyer, Paul Glendenning, Markus P Schlaich, Amanda J Hooper, Damon A Bell

{"title":"Dual-reporting aldosterone by mass spectrometry and immunoassay in the seated saline suppression test may enhance subtype classification of patients with primary aldosteronism.","authors":"Nicola Sawyer, Paul Glendenning, Markus P Schlaich, Amanda J Hooper, Damon A Bell","doi":"10.1016/j.pathol.2025.02.008","DOIUrl":"https://doi.org/10.1016/j.pathol.2025.02.008","url":null,"abstract":"Immunoassay aldosterone measurements are frequently higher than liquid chromatography-tandem mass spectrometry (LC-MS/MS) results, potentially increasing the overdiagnosis of primary aldosteronism (PA) during the seated saline suppression test (SSST). This study determined the rate of concordance between LC-MS/MS and immunoassay aldosterone measurements following SSST and compared the adrenal vein sampling (AVS) lateralisation rates in patients with concordant and discordant SSST results. This retrospective analysis comprised 151 patients who underwent SSST in Western Australia and had dual-reported SSST aldosterone LC-MS/MS and immunoassay results, with 55 patients who proceeded to AVS studies. PA was excluded in 22.5% (34/151) of patients by a post-SSST immunoassay aldosterone result ≤170 pmol/L plus an LC-MS/MS result <120 pmol/L, 32.5% (49/151) had PA confirmed by an immunoassay aldosterone result >170 pmol/L and an LC-MS/MS result >162 pmol/L, and 45.0% (68/151) had an immunoassay result >170 pmol/L, which was discordant with the LC-MS/MS result ≤162 pmol/L. Comparable rates of AVS lateralisation of 82.1% (32/39) and 81.3% (13/16) were present in patients with concordant and discordant SSST results, respectively. In patients with lateralised AVS results, non-classical adrenal histopathology was found in 50.0% (8/16) of adrenalectomised patients with concordant SSST results compared with 88.9% (8/9) with discordant SSST results (p=0.088). Dual-reporting of post-SSST LC-MS/MS and immunoassay aldosterone results may offer additional diagnostic classification in patients being selected for AVS and may identify higher rates of micronodular variants of PA, although further investigation is required.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-HMGCR antibodies can be accurately detected by indirect immunofluorescence on HEp-2000 substrate. 间接免疫荧光法可在HEp-2000底物上准确检测抗hmgcr抗体。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-18 DOI: 10.1016/j.pathol.2025.02.010

Hamish Anderson, Mark Van Voorthuizen, John O'Donnell, Sarah Beck

引用次数: 0

Distinct genetic alterations in CD10-negative MUM1-positive follicular lymphoma. cd10阴性mum1阳性滤泡性淋巴瘤的明显遗传改变。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-17 DOI: 10.1016/j.pathol.2025.01.012

Yan Yang, Wei Wang, Liang Zhang, Xu Lu, Huihui Li, Yixiong Liu, Zhouyi Xu, Danhui Zhao, Jia Chai, Longfei Shao, Linni Fan, Mingyang Li, Yanru Yang, Chuanshu Gao, Haibo Wu, Zhe Wang

{"title":"Distinct genetic alterations in CD10-negative MUM1-positive follicular lymphoma.","authors":"Yan Yang, Wei Wang, Liang Zhang, Xu Lu, Huihui Li, Yixiong Liu, Zhouyi Xu, Danhui Zhao, Jia Chai, Longfei Shao, Linni Fan, Mingyang Li, Yanru Yang, Chuanshu Gao, Haibo Wu, Zhe Wang","doi":"10.1016/j.pathol.2025.01.012","DOIUrl":"https://doi.org/10.1016/j.pathol.2025.01.012","url":null,"abstract":"Conventional follicular lymphoma (FL) is positive for CD10 and negative for multiple myeloma oncogene 1 (MUM1). However, a subset of FL shows CD10-MUM1+ features, and the genetic alterations of this unique FL subtype are poorly understood. Thus, we conducted whole-exome sequencing in 20 cases of CD10-MUM1+ FL and analysed the genetic and clinicopathological features of these patients. The results suggested that CD10-MUM1+ FL patients were predominantly elderly women, lacked BCL2 rearrangement (17/17, 100%) and showed a higher grade (17/20, 85% grade 3) than patients with conventional FL. The most frequently mutated genes in these tumours were CD79B, GOLGA6L2, IGLL5, KMT2D, NFKBIE, ROBO1, BTG2, DTX1, LRP3 and PTPN6. In contrast to conventional FL, CD10-MUM1+ FL exhibited an elevated mutation frequency of CD79B and NFKBIE and decreased mutation frequencies of CREBBP, BCL2, KMT2D and TNFRSF14. No significant difference was found in the overall survival between CD10-MUM1+ FL and conventional FL patients. In summary, this study demonstrated that CD10-MUM1+ FL has unique clinical, pathological and genetic features.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the usefulness of prostate tissue in diagnosing transthyretin amyloidosis and predicting cardiac outcomes: an autopsy-based study. 探讨前列腺组织在甲状腺转蛋白淀粉样变诊断和预测心脏预后中的作用：一项基于尸检的研究。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-15 DOI: 10.1016/j.pathol.2025.02.007

Shojiro Ichimata, Yukiko Hata, Koji Yoshida, Kenichi Hirabayashi, Naoki Nishida

{"title":"Exploring the usefulness of prostate tissue in diagnosing transthyretin amyloidosis and predicting cardiac outcomes: an autopsy-based study.","authors":"Shojiro Ichimata, Yukiko Hata, Koji Yoshida, Kenichi Hirabayashi, Naoki Nishida","doi":"10.1016/j.pathol.2025.02.007","DOIUrl":"https://doi.org/10.1016/j.pathol.2025.02.007","url":null,"abstract":"This study aimed to evaluate the clinicopathological features of prostatic transthyretin-derived amyloid (ATTR) deposition in patients with sporadic cardiac ATTR amyloidosis (sATTR-CA), investigate the correlation between the severity of amyloid deposition in the prostate and heart, and identify prostatic deposition patterns that could predict severe cardiac involvement. We evaluated 35 male autopsy cases with sATTR-CA. The severity of prostatic and cardiac ATTR deposition was assessed semiquantitatively. Using digital pathology, we quantitatively evaluated the immunohistochemical deposition burden of ATTR in the prostatic interstitium and myocardium. Amyloid deposition was detected in 18 cases (51%) in haematoxylin and eosin (H&E)-stained specimens, and ATTR deposition was confirmed in 29 cases (83%) using Congo red staining and immunohistochemistry for transthyretin. The group with identifiable amyloid deposition in the prostate on H&E staining had significantly more severe ATTR deposition in the heart than the group without prostatic deposition. The severity of deposition at all prostatic sites correlated positively with semiquantitative and quantitative deposition severity scores in the heart. Notably, the severity of deposition in the prostatic intraglandular interstitium and extraglandular vessels showed a strong positive correlation with the severity of sATTR-CA. Prostatic ATTR deposition was prevalent and may be a useful organ for diagnosing ATTR deposition. Additionally, the severity of prostatic deposition correlated with the severity of cardiac deposition. As biopsy materials often lack extraglandular tissue, it is crucial not to overlook intraglandular interstitium deposits on H&E staining.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pilocytic astrocytoma with BARD1 mutation: oncogenic pathways via DNA damage repair mechanism and MAPK/BRAF. 伴有BARD1突变的毛细胞星形细胞瘤：通过DNA损伤修复机制和MAPK/BRAF的致癌途径

IF 3.6 3区医学

Pathology Pub Date : 2025-04-09 DOI: 10.1016/j.pathol.2025.02.005

Maher Kurdi, Fadi Toonsi, Alaa Alkhotani, Yazid Maghrabi, Fahad Okal, Maryam Enani, Huda Alghefari, Saleh Baeesa

引用次数: 0

V217F MYD88 mutation in low-grade B-cell lymphomas with plasmacytic differentiation: a diagnostic challenge. 低级别b细胞淋巴瘤伴浆细胞分化的V217F MYD88突变：一个诊断挑战

IF 3.6 3区医学

Pathology Pub Date : 2025-04-07 DOI: 10.1016/j.pathol.2025.02.006

Jesús de Beltrán Machuca Aguado, Rebeca Manso Alonso, Rocío Nieves Salgado, Tamara Castaño Bonilla, Marta León Del Campo, Socorro María Rodríguez Pinilla, Francisco Javier Díaz de la Pinta

引用次数: 0

Are deeper sections and immunohistochemistry useful in detecting micrometastases and isolated tumour cells in colorectal cancer? 深层切片和免疫组织化学对检测结直肠癌的微转移和分离肿瘤细胞有用吗？

IF 3.6 3区医学

Pathology Pub Date : 2025-04-04 DOI: 10.1016/j.pathol.2025.01.011

David W Dodington, Keegan Guidolin, Fayez Quereshy, Runjan Chetty, Stefano Serra, Klaudia M Nowak

{"title":"Are deeper sections and immunohistochemistry useful in detecting micrometastases and isolated tumour cells in colorectal cancer?","authors":"David W Dodington, Keegan Guidolin, Fayez Quereshy, Runjan Chetty, Stefano Serra, Klaudia M Nowak","doi":"10.1016/j.pathol.2025.01.011","DOIUrl":"https://doi.org/10.1016/j.pathol.2025.01.011","url":null,"abstract":"Identification of lymph node metastases is critical for staging of colorectal cancer. Lymph node metastases are classified based on size as macrometastases, micrometastases, or isolated tumour cells (ITCs). Micrometastases are associated with worse prognosis; however, optimal detection methods have yet to be established. The first objective was to determine if deeper levels and immunohistochemistry would detect micrometastases in patients with metastatic disease but negative lymph nodes. Five patients with pT3N0 colorectal adenocarcinoma who developed metastatic disease were identified. Three deeper haematoxylin and eosin (H&E) levels followed by pan-cytokeratin immunohistochemistry was performed on all lymph nodes. No micrometastases were detected; however, ITCs were seen by immunohistochemistry in three of five patients. Driven by these findings, the second objective was to determine if a single level stained for pan-cytokeratin would identify ITCs and if their presence was associated with an increased risk of disease recurrence. A cohort of eight patients with stage IIA (pT3N0M0) colorectal adenocarcinoma who developed distant metastasis was matched to eight control patients who remained disease-free over a 5-year period, and a single pan-cytokeratin stain was performed on all lymph nodes. ITCs were identified in six of eight patients that developed metastasis and in five of eight control patients (odds ratio=1.80; 95% confidence interval=0.21-15.41). In conclusion, three deeper levels and immunohistochemistry did not increase the yield of micrometastases in pT3N0 colorectal adenocarcinoma. While ITCs were readily identified by immunohistochemistry, their presence was not a significant predictor of distant recurrence. These findings do not support the routine use of deeper levels and immunohistochemistry for lymph node staging.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-nucleotide polymorphism (SNP) microarray as an ancillary tool in the classification of bone tumours. 单核苷酸多态性（SNP）微阵列作为骨肿瘤分类的辅助工具。

IF 3.6 3区医学

Pathology Pub Date : 2025-04-03 DOI: 10.1016/j.pathol.2024.11.017

Narelle Barton, Christopher Joy, Alison L Cheah, Fiona Bonar, Fiona Maclean, James Harraway, A Cristina Vargas

引用次数: 0