IF 4.5 3区医学

Pathology Pub Date : 2024-09-07 DOI: 10.1016/j.pathol.2024.08.001

Jane Thompson, Geoffrey Thompson, Deborah White, David Yeung

引用次数: 0

Frequent detection of herpes simplex virus and varicella zoster virus in samples submitted for monkeypox virus testing in New South Wales, Australia during the mpox outbreak 2022–2023 2022-2023 年澳大利亚新南威尔士州猴痘病毒爆发期间，在提交猴痘病毒检测的样本中频繁检测到单纯疱疹病毒和水痘带状疱疹病毒

IF 4.5 3区医学

Pathology Pub Date : 2024-08-26 DOI: 10.1016/j.pathol.2024.06.011

Maurizio Stefani, Justin Ellem, Neisha Jeoffreys, Jimmy Ng, Dominic E. Dwyer, Sharon C-A. Chen, Jen Kok

引用次数: 0

Mutations of cysB in urinary isolates of cysteine-requiring Escherichia coli 尿液中分离出的需半胱氨酸大肠埃希菌中 cysB 的突变

IF 4.5 3区医学

Pathology Pub Date : 2024-08-24 DOI: 10.1016/j.pathol.2024.06.009

Ryanbi Pratama, Peter C. Taylor, Chinmoy Mukerjee, Christopher J. McIver

引用次数: 0

Ossifying fibromyxoid tumour with fibrosarcoma-like features and novel PHF1::HCFC1 gene fusion 具有纤维肉瘤样特征和新型 PHF1::HCFC1 基因融合的骨化纤维瘤

IF 4.5 3区医学

Pathology Pub Date : 2024-08-24 DOI: 10.1016/j.pathol.2024.06.010

Gideon Ze Lin Tan, Jian Yuan Goh, Clarence Jia Jun Yen, Mark Edward Puhaindran, Yingting Mok

引用次数: 0

Comparison of PD-L1 assays in head and neck carcinoma 头颈癌中 PD-L1 检测方法的比较

IF 4.5 3区医学

Pathology Pub Date : 2024-08-22 DOI: 10.1016/j.pathol.2024.06.006

Ji-Seon Jeong, Uiree Jo, Gyuheon Choi, Halim Song, Kyung-Ja Cho, Joon Seon Song

{"title":"Comparison of PD-L1 assays in head and neck carcinoma","authors":"Ji-Seon Jeong, Uiree Jo, Gyuheon Choi, Halim Song, Kyung-Ja Cho, Joon Seon Song","doi":"10.1016/j.pathol.2024.06.006","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.06.006","url":null,"abstract":"Programmed cell death-ligand 1 (PD-L1) expression is a predictive biomarker for immune checkpoint inhibitor in head and neck squamous cell carcinoma. Given the range of antibodies and platforms for PD-L1 testing, it is essential to understand the performance of different staining and scoring methods. PD-L1 expression in 156 head and neck mucosal squamous cell carcinoma (HNmSCC) cases at Asan Medical Center was assessed using 106 TMA cores and 50 whole slides. Three standardised PD-L1 assays (22C3 pharmDx, SP263, and 28-8 pharmDx) and one laboratory-developed test (22C3 LDT) were evaluated: the combined positive score (CPS) with ≥1, ≥20, and ≥50 cut-offs, and the tumour positive score (TPS) with ≥1%, ≥20%, ≥50% cut-offs. Concordance on a continuous scale among the assays was good to excellent for CPS [intraclass correlation coefficient (ICC) range 0.73–0.94] and TPS (ICC range 0.70–0.94) and in both TMA and whole slides cohorts. Stratification by variable cut-offs demonstrated moderate to good agreement among most assays, as analysed by Gwet's AC1. PD-L1 expression was significantly correlated with tumour location using the 22C3 pharmDx assay (CPS, =0.014; TPS, =0.033). Notable concordance was found among PD-L1 assays, suggesting their potential interchangeability in HNmSCC.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":null,"pages":null},"PeriodicalIF":4.5,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142205227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update on methods used for mycological testing: wide diversity and opportunities for improvement persist. 真菌学检测所用方法的最新情况：种类繁多，仍有改进机会。

IF 3.6 3区医学

Pathology Pub Date : 2024-08-18 DOI: 10.1016/j.pathol.2024.06.007

Arthur J Morris, Sarah E Kidd, Catriona L Halliday, Sharon C-A Chen, Wendy McKinney, Katherine Ryan, Juliet Elvy

{"title":"Update on methods used for mycological testing: wide diversity and opportunities for improvement persist.","authors":"Arthur J Morris, Sarah E Kidd, Catriona L Halliday, Sharon C-A Chen, Wendy McKinney, Katherine Ryan, Juliet Elvy","doi":"10.1016/j.pathol.2024.06.007","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.06.007","url":null,"abstract":"Past analysis of laboratory methods used for mycology specimens revealed significant variation in practices, many of which fell short of recommended procedures. In 2016 these findings led to a set of recommendations for laboratories to consider modification of their methods where appropriate, to analyse current laboratory methods used by participants in the Royal College of Pathologists of Australasia Quality Assurance Programs (RCPAQAP) Mycology module, and to compare these to the 2016 recommendations. Seven test items, with 105-107 participants each, were analysed. Several laboratories (7-12%) did not handle specimens as recommended in an appropriate biological safety cabinet. Direct microscopy was not performed on tissue specimens 23-25% of the time. The most used staining method was potassium hydroxide with an optical brightener for fluorescent microscopy (49%) followed by Gram stain (33%). While 17-25% of laboratories used three or more media, use of four or more was uncommon (<3%). Between 9-13% of participants used only a single non-inhibitory medium for cultures. Urine specimens were incubated longer than recommended with 57% of laboratories incubating for >7days and 24% >21 days. Duration of incubation was shorter than recommended for several specimen types with 36% of skin specimens and 37-48% of tissue specimens being kept ≤21 days. For cultures kept >7 days, 13% were inspected daily but for those incubating >14 days only 3%. The methods of several laboratories remain outside recommended practice. An updated set of recommendations are made.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142110739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of lentiginous junctional melanocytic naevus, toker cell hyperplasia and pagetoid dyskeratosis of the nipple: a potential diagnostic pitfall 乳头皮样交界性黑素细胞痣、角化细胞增生症和页状角化异常的合并症：潜在的诊断陷阱

IF 4.5 3区医学

Pathology Pub Date : 2024-08-18 DOI: 10.1016/j.pathol.2024.06.008

Mark James Wilsher, James D. Bedford

引用次数: 0

The effect of Proteinase K treatment on GeoMx digital spatial profiling data quality from formalin-fixed, paraffin-embedded tissue. 蛋白酶 K 处理对来自福尔马林固定、石蜡包埋组织的 GeoMx 数字空间剖析数据质量的影响。

IF 3.6 3区医学

Pathology Pub Date : 2024-08-14 DOI: 10.1016/j.pathol.2024.06.004

Kyle M Hatton-Jones, Nicholas P West, Jean Barcelon, Amanda J Cox

{"title":"The effect of Proteinase K treatment on GeoMx digital spatial profiling data quality from formalin-fixed, paraffin-embedded tissue.","authors":"Kyle M Hatton-Jones, Nicholas P West, Jean Barcelon, Amanda J Cox","doi":"10.1016/j.pathol.2024.06.004","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.06.004","url":null,"abstract":"The emergence of spatial profiling technologies in recent years has accelerated opportunities to profile in detail the molecular attributes of a wide range of tissue pathologies using archival specimens. However, tissue treatment for fixation and storage does not always support generation of high-quality genomic data. The purpose of this study was to investigate the impacts of Proteinase K (ProtK) treatment, as a way to increase target transcript exposure, on downstream sequencing data quality metrics for spatial transcriptomic data using formalin-fixed, paraffin-embedded samples. In a series of four independent assessments using different tissue types (nasal mucosa, tonsil, pancreas), varying concentrations of ProtK (ranging from 0.1 to 1 μg/mL) were used as part of the sample processing workflow to generate transcriptomic data using the Nanostring GeoMx DSP and Illumina NextSeq 2000 platforms. Use of higher concentrations of ProtK was generally found to increase total reads (2-4-fold). However, negative probe counts also tended to be increased (2-12-fold), resulting in reductions in the signal-to-noise ratio (10-70% lower) and the number of genes detected above background (50-80% lower). These effects were not seen in all tissues and impacts of tissue handling and processing, beyond ProtK treatment, on data quality metrics, also require consideration. Regardless, these observations highlight the need for careful consideration of a range of sample processing factors and benefits that may be achieved through the optimisation of sample processing workflows for specific tissues as a way to maximise the generation of quality data using spatial transcriptomic approaches.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142126376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lupoid cutaneous leishmaniasis in Pakistan: a case series in school children. 巴基斯坦的鳞状皮肤利什曼病：学龄儿童病例系列。

IF 3.6 3区医学

Pathology Pub Date : 2024-08-14 DOI: 10.1016/j.pathol.2024.06.005

Asma Ashraf, Saima Qadeer, Ume Amara Bukhari, Umme Salma

引用次数: 0

ALK-positive large B-cell lymphoma: a clinicopathological and molecular characteristics analysis of seven cases. ALK阳性大B细胞淋巴瘤：七例病例的临床病理学和分子特征分析。

IF 3.6 3区医学

Pathology Pub Date : 2024-08-13 DOI: 10.1016/j.pathol.2024.05.014

Xuan Wang, Hongmei Yi, Qingxiao Liu, Tuanjie Guo, Anqi Li, Binshen Ouyang, Yimin Li, Yuxiu Zhang, Haimin Xu, Lei Dong, Xu Wang, Chaofu Wang

{"title":"ALK-positive large B-cell lymphoma: a clinicopathological and molecular characteristics analysis of seven cases.","authors":"Xuan Wang, Hongmei Yi, Qingxiao Liu, Tuanjie Guo, Anqi Li, Binshen Ouyang, Yimin Li, Yuxiu Zhang, Haimin Xu, Lei Dong, Xu Wang, Chaofu Wang","doi":"10.1016/j.pathol.2024.05.014","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.05.014","url":null,"abstract":"Anaplastic lymphoma kinase-positive large B-cell lymphoma (ALK+ LBCL) is a rare and highly aggressive lymphoma with characteristic ALK rearrangements. Various fusion genes involving ALK have been demonstrated, but the influence of the ALK fusion partners on ALK protein expression and the genetic characteristics of ALK+ LBCL remain relatively unknown. In this study, we conducted an extensive clinicopathological and molecular analysis on seven cases of ALK+ LBCL to explore the correlation between ALK fusion genes and ALK protein expression, thereby enriching the genetic characteristics of this tumour. We integrated the findings from clinical, histopathological/immunophenotypic, and molecular studies, including three samples subjected to next-generation sequencing, and six cases underwent RNA-based ALK fusion gene detection. We identified five distinct types of ALK fusion genes, including CLTC, NPM1, PABPC1, SEC31A, and TFG. Notably, only the NPM1::ALK fusion showed nuclear and cytoplasmic ALK staining, and the remaining four fusion genes resulted in cytoplasmic ALK staining. Our analysis revealed that the CLTC::ALK fusion resulted in a unique cytoplasmic perinuclear Golgi zone focal granular heterogeneous staining pattern of ALK. Additionally, we identified six potentially clinically significant gene mutations, including TET2, CHD2, DTX1, KMT2D, LRP1B, and XPO1. Furthermore, in all cases, the absence of 5-hydroxymethylcytosine (5hmC) was observed. We present seven cases of ALK+ LBCL, discussing the correlation between fusion genes and ALK protein expression, and enhancing our understanding of the genetic attributes of this tumour. This study also shows the loss of 5hmC in nearly all seven ALK+ LBCL cases, independently of TET2 mutations.","PeriodicalId":19915,"journal":{"name":"Pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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