Pathology最新文献

{"title":"Encapsulating the diagnostics of cryptococcal infections: what to do when there is minimal capsule.","authors":"Ravin Hettiarachchi, Michael Han, Mark Krockenberger, Louella Davey, Pamela Konecny, Robert Stevens, Chris Weatherall","doi":"10.1016/j.pathol.2024.12.646","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.646","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microvascular density using the Chalkley method: a potential alternative to the World Health Organization nuclear grading in pleural mesothelioma prognostication.","authors":"Revania Pillay, Ashleigh J Hocking, Sarita Prabhakaran, Sonja Klebe","doi":"10.1016/j.pathol.2024.12.643","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.643","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel mutation in acaeruloplasminaemia: a rare case of neurodegeneration mimicking normal pressure hydrocephalus.","authors":"Man-Kwan Yip, Hoi Kevin Chin, Man Au Yeung, Wing-Tat Poon","doi":"10.1016/j.pathol.2024.12.644","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.644","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modified cut-off index for the Elecsys Anti-SARS-CoV-2 nucleocapsid electrochemiluminescence immunoassay.","authors":"Caitlin Hooker, Brittany Lavender, Chris Frampton, Sian Horan, James Ussher, Maia Brewerton","doi":"10.1016/j.pathol.2024.12.642","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.642","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep nabothian cysts: an underappreciated phenomenon and mimic of cervical gastric-type adenocarcinoma.","authors":"Stephanie N J Chapple, Rachel Maywald, Niveditha Rajadevan, Karen L Talia, W Glenn McCluggage","doi":"10.1016/j.pathol.2024.12.645","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.645","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary disseminated varicella infection: a mimicry of cutaneous cytotoxic T-cell lymphoma.","authors":"An Jian Leung, Siok-Bian Ng, Meiqi May Liau, Kong Bing Tan","doi":"10.1016/j.pathol.2024.12.640","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.640","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A complex case of mixed-phenotype acute leukaemia B/T/myeloid.","authors":"Nourhan Ibrahim, Zubaidah Al-Jumaili, Phuoc T Christie-Nguyen, Sibel Ak, Brenda Mai","doi":"10.1016/j.pathol.2024.12.641","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.641","url":null,"abstract":"","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recycled formalin: a new tool to mitigate the environmental impact of surgical pathology in routine practice.","authors":"Anne Rullier, Samuel Amintas, Maxime Marques, Brigitte Le Bail, Geneviève Belleannée, Pierre Dubus, Rémi Vergara, Noelle Bernard","doi":"10.1016/j.pathol.2024.12.639","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.639","url":null,"abstract":"<p><p>Formalin is universally acknowledged as the gold standard for tissue fixation. However, it is a dangerous toxic chemical without any realistic substitute. Therefore, decreasing its use seems to be the only way to reduce its environmental impact. We assessed the feasibility of an innovative formalin recycling circuit based on the reuse of formalin following biopsy removal and paper filtration. We also assessed the efficiency of a policy of less formalin use in routine practice without compromising work quality and security. The recycled formalin was equivalent to new formalin in terms of its chemical and molecular properties, as well as the fixation, analysis, and storage of tissue samples. Moreover, its use for fixation did not affect molecular analyses. We calculate that our process would have decreased the total consumption of formalin in 2022 by 26% compared to 2021. This corresponds to financial savings of €4620, a 2434 kg CO₂ equivalent reduction in greenhouse gases, and substantial decreases in toxicity to humans and freshwater. Our recycled formalin circuit is a simple, easy-to-implement, efficient way to mitigate the environmental impact of formalin in surgical pathology without compromising the quality of analysis and the safety of the pathology staff.</p>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cost-benefit analysis of two quality control approaches for infectious disease testing.","authors":"Wayne Dimech, Patricia Mitchell, Giuseppe Vincini","doi":"10.1016/j.pathol.2025.01.002","DOIUrl":"https://doi.org/10.1016/j.pathol.2025.01.002","url":null,"abstract":"&lt;p&gt;&lt;p&gt;The traditional methods for monitoring quality control (QC) results of using the mean and standard deviation of 20 external QC results to establish acceptance criteria have been widely accepted in medical pathology. The use of Westgard rules to monitor assay performance has been used by clinical chemists for decades and is now applied to infectious disease testing. However, previous reports have indicated this approach creates frequent 'false rejections' leading to a waste of time and resources. This study evaluated the true cost of false rejections in a single laboratory by mapping the QC activities over a 5-month period. From January 2023 to May 2023 inclusive, a laboratory logged all QC results that failed Westgard rules using Bio-Rad Unity software. All activities arising from those QC failures were logged and the cost calculated in Australian dollars. The costs included the cost of reagents, external quality control (EQC), kit control and calibrators, and the staff time. As each laboratory has different pricing structures, certain assumptions were made. The laboratory used the traditional methods for patient result release. Over the same period, the EQC results were submitted to EDCNet software and subjected to National Serology Reference Laborator-developed QConnect Limits. At the end of the period, the outcomes of the traditional approach to EQC monitoring were compared with QConnect Limits. The senior scientist identified which QC rejections were 'true rejections'.Over the 5-month period, there were a total of 70 flags raised across 15 of the 23 test kits used on two different test platforms. All but one of the 70 episodes resulted in repeat testing of the EQC sample. On 17 occasions, the QC sample was tested more than three times. Recalibration of the assay occurred 12 times, and the results were referred to the senior scientist six times. The acceptance criteria for the EQC were re-set seven times. After ensuing investigations, only one of the 70 flags was deemed to be a 'real error' by the senior scientist. This was also detected by QConnect Limits. The cost of EQC flag investigations over the 5-month period was calculated to be just under $12,000 for 5 months. Extrapolating this to a 12-month period, the additional cost to the laboratory for EQC follow-up was estimated to be approximately $28,700 or about $2,400 per month. Delays in the release of patient results occurred in 42 of 70 episodes. In total, over the 5-month period, the accumulated delay in patients' reports was 68 h ​or 816 min per month. The use of traditional methods for the monitoring EQC is not fit for purpose of infectious disease testing. This is because frequent, normal reagent lot-to-lot variation is expected in serology tests. These changes cause frequent 'false rejections' if the acceptance criteria are too tight, as is the case when using 20 data points to establish limits. The consequences of false rejections are the waste of resources and staff time, ad","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of fibroblast activation protein (FAP) in the stroma of proliferative inflammatory atrophy (PIA) and primary adenocarcinoma of the prostate.","authors":"Fernanda Caramella-Pereira, Qizhi Zheng, Jessica L Hicks, Sujayita Roy, Tracy Jones, Martin Pomper, Lizamma Antony, Alan K Meeker, Srinivasan Yegnasubramanian, Angelo M De Marzo, W Nathaniel Brennen","doi":"10.1016/j.pathol.2024.12.637","DOIUrl":"https://doi.org/10.1016/j.pathol.2024.12.637","url":null,"abstract":"<p><p>Fibroblast activation protein (FAP) is a serine protease upregulated at sites of tissue remodelling and cancer that represents a promising therapeutic and molecular imaging target. In prostate cancer, studies of FAP expression using tissue microarrays are conflicting, such that its clinical potential is unclear. Furthermore, little is known regarding FAP expression in benign prostatic tissues. Here we demonstrated, using a novel iterative multiplex immunohistochemistry assay in standard tissue sections, that FAP was nearly absent in normal regions ​but was increased consistently in regions of proliferative inflammatory atrophy (PIA). In carcinoma, FAP was expressed in all cases ​but was highly heterogeneous. High FAP levels were associated with increased pathological stage and cribriform morphology. We verified that FAP levels in cancer correlated with CD163+ M2 macrophage density. In this first report to quantify FAP protein in benign prostate and primary tumours, using standard large tissue sections, we clarify that FAP is present in all primary prostatic carcinomas, supporting its potential clinical relevance. The finding of high levels of FAP within PIA supports the injury/regeneration model for its pathogenesis and suggests that it harbours a protumourigenic stroma, yet ​high levels of FAP in benign regions could lead to false-positive FAP-based molecular imaging results in clinically localised prostate cancer.</p>","PeriodicalId":19915,"journal":{"name":"Pathology","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}