Transactions of Papanin Institute for Biology of Inland Waters RAS最新文献

{"title":"NEW FINDINGS OF CULTURED MICROMYCETES IN THE DEEP-SEA SEDIMENTS OF THE BLACK SEA","authors":"N. Kopytina, E. Bocharova, L. Gulina","doi":"10.47021/0320-3557-2024-45-53","DOIUrl":"https://doi.org/10.47021/0320-3557-2024-45-53","url":null,"abstract":"On August 24, 2011, during the cruise of R/V Professor Vodyanitsky in the Black Sea, bottom sediments were sampled from the hydrogen sulfide zone at a depth of 756 m at the station with coordinates 44°40ʹ70\"N, 31o51ʹ70\"E. The sample was taken using an automatic “Shelf” box corer (United States). A sediment core of 40 cm high was cut out of the box corer. The sediment core is further cut horizontally in 5 cm (8 samples). The sediment is homogeneous dark gray silt with thin white stripes and the smell of hydrogen sulfide. Eighteen species of fungi were found in sediments, 3 taxa were identified to genus level, the taxa were assigned to 11 genera, 10 families, 10 orders, 7 classes from the phylums Ascomycota, Basidiomycota and Mucoromycota. Representatives of the classes Eurotiomycetes (8) and Dothideomycetes (4) dominated in the taxonomic composition of mycocomplexes of sediments in the Black Sea. The abundance of micromycetes varied from 40 (horizon 15–20 cm) to 3300 CFU g-1 dry sediment (horizon 5–10 cm); the maximum number of taxa (5) was recorded \u0000in the horizon of 25–30 cm. The representatives of the phylum Ascomycota dominated in the samples in terms \u0000of the number of taxa – 91.23%.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"14 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140969306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYCOBIOTA IN THE BOTTOM GROUNDS OF THE GORYACHINSKY THERMAL SPRING","authors":"A. Kurakov, A. Tsarelunga","doi":"10.47021/0320-3557-2023-72-91","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-72-91","url":null,"abstract":"The number of colony-forming units (CFU), composition and taxonomic structure of fungal biota in the bottom grounds of the Goryachinsk geothermal spring (Buryatia, Russian Federation) were determined by the cultural approach and the method of high-performance sequencing. The number of fungi in the grounds (0–3 cm) at the outlet of the spring and the watercourse at a short distance (3–100 m) varied in the range from several units to hundreds of CFU in 1 g. 70 isolates of fungi of 34 morphotypes were isolated by platings on nutrient media, of which 15 were identified to the species and 2 to the genus level. Its were thermophilic and thermotolerant species Aspergillus fumigatus, A. niger, A. nishimurae, A. terreus, Melanocarpus albomyces, Mycothermus thermophilus, Naganishia brisbanensis, Penicillium sp., Pseudothielavia terricola, Rhodotorula sp., Scedosporium apiospermum, Talaromyces flavus, Thermomyces dupontii, Thermothielavioides terrestris and Vishniacozyma carnescens. The method of high-performance sequencing of the ITS2 rDNA site in the source soils revealed an order of magnitude greater diversity of fungi, 149 species of 132 genera, and a total of 160 species were detected by both approaches. The number of identified operational-taxonomic units (OTE) to the species was 64.4%. In addition to ascomycetes and basidiomycetes, which were detected by platings on nutrient media, DNA barcoding showed the presence of representatives of the Phylums Mucoromycota, Mortierellomycota, Zoopagomycota, Chytridiomycota, Rozellomycota in the grounds. Moreover, among the fungi established by high-performance sequencing were not only thermotolerants, but also species with different resistance to high temperatures and trophic orientation. The application of both approaches provided more detailed information about the diversity of fungal organisms in the hot spring. However, in order to identify the inhabitant species of such ecotopes, a thorough analysis of their physiological and biochemical properties (which for many taxa is absent in due to volume) and the use of other approaches are necessary.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"13 44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FUNGAL COMPLEXES ON CELLULOSE-CONTAINING SUBSTRATES IN COASTAL AND DEEP-WATER AREAS OF THE BLACK SEA","authors":"N. Kopytina, E. Bocharova","doi":"10.47021/0320-3557-2023-28-39","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-28-39","url":null,"abstract":"","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DISTRIBUTION AND ROLE OF FUNGI AND FUNGUS-LIKE ORGANISMS IN ZOOPLANKTON OF FRESHWATER ECOSYSTEMS (REVIEW)","authors":"L. Voronin, S. Zhdanova","doi":"10.47021/0320-3557-2023-7-16","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-7-16","url":null,"abstract":"Information on the species composition and distribution of zooplankton mycoparasites is summarized: 8 species of Chytridiomycota, 2 – Ascomycota, 7 – microsporidia, 19 – fungal-like organisms Oomycota. Known life cycles, dependence of infection spread on water temperature, density of host and parasite populations, host are given. The presence of saprotrophic species in plankton is indicated. They feed on dead inhabitants of plankton and introduced invertebrate corpses from terrestrial conditions: 30 species of Chytridiales, 4 – Mucorales and 35 species of fungus-like Saprolegniales. Information on the role of fungi in the nutrition of zooplankton is given, mainly zoospores and mitospore ascomycetes.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FUNGI ASSOCIATED WITH NUPHAR LUTEA (L.) SMITH IN THE KOTOROSL RIVER (YAROSLAVL, RUSSIA)","authors":"L. Voronin","doi":"10.47021/0320-3557-2023-23-27","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-23-27","url":null,"abstract":"Studies of mycobiota of Nuphar lutea leaves in the estuary part of the Kotorosl River, located in the center of a large city, showed that phylloplane fungal complexes in 2006, 2007 and 2021 years form and consist of a small number of more or less constant species and a large number are temporary. Fungi and fungal-like organisms in all cases become participants in the primary stages of destructions of dead leaves. These are some inhabitants of phylloplane complexes, spotable to switch to feeding on leaf tissues, and new colonizers, the life cycle of which completely occurs in water in 2006 and 2007 years. In 2021 we found only poorly developed mycelium. Antropogenic impact on the river affects the species composition, and most importantly – the intensity of grows and the passage of the full life cycle of micodestructors.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHANGES IN MACROPHYTE MYCODESTRUCTOR COMPLEXES IN URBAN WATER BODY (YAROSLAVL, RUSSIA)","authors":"L. Voronin, P. Chernyavskaya","doi":"10.47021/0320-3557-2023-17-22","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-17-22","url":null,"abstract":"The results of mycodestructors on dead fragments of common reeds and river horsetail study in a small lake-type reservoir in 1998–1999 and 2021–2022 are presented. The method of accumulative culture in 1998–1999 recorded 18 species on reeds (8 Ascomycota teleomorphs, 6 mitospore, 4 Oomycota), 12 species on horsetails (1 Blastocladiomycota, 1 Ascomycota teleomorphs, 3 mitospore, 7 Oomycota). True aquatic (Hymenoscyphus albidus, Massarina fluviatilis, M. phragmiticola, Mollisia hydrophila) and capable of developing teleomorphs and anamorphs of ascomycetes in water have been identified. Mycobiota corresponded to the mesotrophic type of reservoir. In 2021–2022, 4 species were recorded on the reed, 5 – on the horsetail. Fungi-like oomycetes dominated, from genera Pythium and Saprolegnia, they are able to develop in reservoirs with a high concentration of dissolved organic matter and with stand a contaminated environment. Over two decades, according to mycological indicators, the lake changed the trophic status of mesotrophic to eutrophic, which corresponds to eutrophic type of overgrown reservoir with antropogenic pollution.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"REMEDIATION POTENTIAL OF AQUATIC FUNGI","authors":"E. Fedoseeva, V. Terekhova","doi":"10.47021/0320-3557-2023-50-71","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-50-71","url":null,"abstract":"The review is devoted to the analysis of information on aquatic fungi as potential agents of bioremediation of aquatic environments under chemical pollution. Approaches to the classification of groups of aquatic mycobiota based on the duration of existence in aquatic environments and morphophysiological features of species are considered. The known mechanisms of interaction between fungi and pollutants, which result in biodegradation or biosorption of pollutants, and ultimately a decrease in the concentration of chemicals available to other inhabitants of aquatic environments, are outlined. Specific examples illustrating the role of fungal enzymes in these processes are considered. Data are given on the use of fungal strains isolated from marine and fresh waters for the purpose of mycoremediation, and the effectiveness of fungi of different origin in bioremediation is characterized. It is concluded that a more promising direction of microremediation seems to be the orientation towards the choice of biodestructor fungi species that have specific enzymes that can lead to the degradation of pollutants.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MICROPLANKTON STRUCTURE IN THE VOLGA RIVER WATER ON THE SITE OF THE VILLAGE OF PRILUKI – THE VILLAGE OF KRASNY PROFINTERN (YAROSLAVL REGION)","authors":"N. Kopytina, I. Rybakova","doi":"10.47021/0320-3557-2023-40-49","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-40-49","url":null,"abstract":"In August 2021, in the channel part of the Volga River, within the river stretch between Priluki village and Krasnyi Profintern townlet, studies of bacterio- and mycoplankton have been carried out. The study was conducted at 25 stations.The quantitative structure of communities of heterotrophic microorganisms is presented by individual stations and by groups of stations identified by the type of anthropogenic load: “across from a settlement”, “lower border of a settlement”, “5 km downstream from a settlement”, “10 km downstream from a settlement”, “water intake”. The water quality was assessed. Saprophytic bacteria were isolated by the pour-plate method, the total number of bacteria and fungal propagules was determined by direct counting using an epifluorescence microscope. The total number of bacteria at the stations ranged 98.65–99.97% (on average 98.63%) and exceeded the fungi number by 73.0–3824.4 times (on average 1177.8 times). The bacterial biomass amounted to 90.0–99.86% (on average 98.63%) and exceeded the fungal one by 9.0–663.8 times (on average 168 times). In the structure of mycobiota, the mycelium biomass ranged 46.56–96.82% (on average 62.06%), which indicates the functional activity of fungi. Cluster analysis of the quantitative structure of microbial communities, for individual stations, revealed their high similarity: the values of the Bray-Curtis coefficients for number ranged 74.49–99.21%; for biomass – 71.03–98.34%. Cluster analysis of microbial communities by station groups also revealed their significant similarity: in number – 86.6–98.3%; in biomass – 88.20–98.87%. For groups of stations, a high correlation was found between the biomass of bacteria and fungi – –0.85, which indicates different environmental conditions favorable for the development of these microorganisms. The water quality in the station groups was appraised as transitional from polluted to dirty waters, from β-mesosaprobic to polysaprobic, and in trophicity, from mesotrophic to eutrophic.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139338537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INTRA-ANNUAL VARIABILITY OF PLANKTON COMMUNITIES IN THE ESTUARY REGION OF THE SEVERNAYA DVINA RIVER","authors":"I. Makedonskaya, E. Medvedeva, N. Otchenash, I. Studenov, A. Tortsev, Yu. Konina","doi":"10.47021/0320-3557-2023-65-80","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-65-80","url":null,"abstract":"The paper summarizes the results of monthly studies of phyto- and zooplankton communities of the estuarine region of the Northern Dvina, conducted during environmental monitoring from November 2020 to January 2022 at the water intakes of JSC “Arkhangelsk Pulp and Paper Mill” (Novodvinsk) and LLC “RVC-Arkhangelsk” (Arkhangelsk and Silikatchikov settlement). Based on the results of plankton surveys, the qualitative composition was studied and the intra-annual variability of quantitative indicators and structural features of plankton communities was described. In phytoplankton, 273 intraspecific taxa of microalgae were found, among which diatoms predominated (159 taxa). The indicators of abundance and biomass changed synchronously during the entire observation period. Primary production and P/B coefficients were at the level of digital values for the Arctic zone. The Shannon Index identified the phytoplankton community as medium-complex in a stable state. 45 species belonging to 4 systematic groups were found in zooplankton. Seasonal dynamics of zooplankton was characterized by 2 peaks of abundance and biomass – in November 2020 and July 2021. By species composition, the zooplankton community in the water intake areas in February–June 2021 it can be characterized as a kladocern, in July – as a rotifer-kladocern-copepod, in August as a rotifer-kladocern, and December can be considered the end of the growing season. \u0000The degree of contamination was assessed according to the index of saprobity of phytoplankton and zooplankton. It was found that the state of the waters of the Severnaya Dvina River in the study area corresponded to the oligo-β-mesosaprobic zone or class II water quality (moderately polluted).","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126839139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASSOCIATIONS OF FUNGI AND NEMATODES IN THE BLACK SEA","authors":"N. Kopytina, N. Sergeeva","doi":"10.47021/0320-3557-2023-36-46","DOIUrl":"https://doi.org/10.47021/0320-3557-2023-36-46","url":null,"abstract":"For the first time, associations of microscopic fungi and nematodes were found in bottom sediments, periphyton and on driftwood in the Black Sea. Antagonistic relationships between microscopic fungi and nematodes are considered: fungi and fungal-feeding nematodes; nematophagous fungi and nematodes. In laboratory conditions, fungal-feeding nematodes remain viable for 1.5 to 9 months in the presence of 22 species of micromycetes from 20 genera, 11 families, 8 orders, 5 classes, and the phylum Ascomycota. The representatives of the family Halosphaeriaceae dominated in terms of the number of species in the species composition of fungi (9). Five species of fungi were found as part of the associations in bottom sediments and 21 species on driftwood. It is proved that the fruiting bodies of ascomycetes Corollospora maritima, C. trifurcata, Halosphaeriopsis mediosetigera with spores can be the only food source for nematodes Viscosia minor, Oncholaimus sp., Monhystera sp. Micromecetes Corollospora maritima, C. trifurcata, Halosphaeriopsis mediosetigera prevailed in the composition of associations on the driftwood, 16.67–20.83%. The paper presents 19 fungi which were for the first time identified in such associations. Epi- and endobiont fungi were detected during microscopic analysis of nematodes after their fixation and making constant microscopic preparations, but it was impossible to identify exactly their taxonomic affiliation. The nematode Anticoma pontica from the fouling in an underground canal inside Mount Tavros (Balaklava Bay, Sevastopol) was affected by a fungal ectoparasite similar to Drechmeria sp. (phyllum Ascomycota). The nematode Axonolaimus setosus from the sediments on the shelf of the western Crimea from a depth of 83.5 m was apparently infected with a fungal-like organism from the phylum Oomycota. Individuals of A. setosus with hyphae of fungi in the inner cavity and on the cuticle (Fungi sp.) were found in the area of the Bosphorus Strait at a depth of 250 m (hydrogen sulfide zone). The state of morphological and anatomical structures of worms indicates that they were affected by fungi during their lifetime.","PeriodicalId":198921,"journal":{"name":"Transactions of Papanin Institute for Biology of Inland Waters RAS","volume":"7 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120927278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}