Peptide Science最新文献_第7页

Thermally‐controlled spherical peptide gel architectures prepared using the pH switch method 用pH开关法制备热控球形肽凝胶结构

IF 2.4 4区生物学

Peptide Science Pub Date : 2023-02-22 DOI: 10.1002/pep2.24304

Sawsan Almohammed, M. Kanoun, S. Goumri‐Said, M. Alam, Agata Fularz, Abdullah Alnaim, J. Rice, B. Rodriguez

{"title":"Thermally‐controlled spherical peptide gel architectures prepared using the pH switch method","authors":"Sawsan Almohammed, M. Kanoun, S. Goumri‐Said, M. Alam, Agata Fularz, Abdullah Alnaim, J. Rice, B. Rodriguez","doi":"10.1002/pep2.24304","DOIUrl":"https://doi.org/10.1002/pep2.24304","url":null,"abstract":"Self‐assembling nanostructured peptide gels are promising materials for sensing, drug delivery, and energy harvesting. Of particular interest are short diphenylalanine (FF) peptides modified with 9‐fluorenylmethyloxycarbonyl (Fmoc), which promotes the association of the peptide building blocks. Fmoc‐FF gels generally form fibrous networks and while other structures have been demonstrated, further control of the gelation and resulting ordered three‐dimensional structures potentially offers new possibilities in tissue engineering, sensing, and drug release applications. Herein, we report that the structure tunability of Fmoc‐FF gels can be achieved by controlling the water content and the temperature. We further explore the incorporation of metal nanoparticles in the formation of the gel to enable optical sensing applications based on hybrid Fmoc‐FF‐nanoparticle microspheres. Finally, fluorescence lifetime imaging microscopy reveals a correlation between lifetime and reduced bandgap, in support of a semiconductor‐induced charge transfer mechanism that might also increase the stability of an excited state of a probe molecule. The observations potentially further widen the use of these peptide materials in bioimaging and sensing applications.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48688180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Current Peptide Vaccine and Immunotherapy Approaches Against Alzheimer's Disease. 目前针对阿尔茨海默病的多肽疫苗和免疫疗法。

IF 1.9 4区生物学

Peptide Science Pub Date : 2023-01-01 Epub Date: 2022-06-24 DOI: 10.1002/pep2.24289

Chelsea Marie T Parrocha, James S Nowick

引用次数: 0

Temperature‐induced structural change of integrin αvβ3 receptor and its interaction with the RGD peptide ligand 温度诱导的整合素αvβ3受体结构变化及其与RGD肽配体的相互作用

IF 2.4 4区生物学

Peptide Science Pub Date : 2022-12-28 DOI: 10.1002/pep2.24302

Anqi Wang, Kai Yue, Yiang Wei, Weishen Zhong, Genpei Zhang

{"title":"Temperature‐induced structural change of integrin αvβ3 receptor and its interaction with the RGD peptide ligand","authors":"Anqi Wang, Kai Yue, Yiang Wei, Weishen Zhong, Genpei Zhang","doi":"10.1002/pep2.24302","DOIUrl":"https://doi.org/10.1002/pep2.24302","url":null,"abstract":"The structural change of receptor protein at high temperatures is one of the factors affecting the targeting ability of ligand‐installed nanocarriers for combined therapy of hyperthermia and drug delivery. In this study, the binding behaviors and mechanisms of integrin αvβ3 receptor and the arginine‐glycine‐aspartic acid (RGD) peptide ligand at high temperatures were investigated both theoretically and experimentally. The structural parameters of integrin αvβ3 at different temperatures and the interaction forces between the RGD peptide and integrin αvβ3 at different binding sites were calculated by molecular dynamics simulation. Fourier transform infrared spectroscopy, energy dispersive spectroscopy, ultraviolet–visible absorption spectroscopy, and atomic force microscopy were used to analyze the structural changes of integrin αvβ3 and to measure the ligand‐receptor interaction. Results show that the number of hydrogen bonds decreased and the secondary structure of integrin αvβ3 changed with the increase in temperature, indicating the denaturation of integrin αvβ3. The structural stability of the integrin αv subunit was better than that of the integrin β3 subunit at high temperatures. The interaction between the RGD peptide and integrin αvβ3 weakened as the temperature increased because the structure of the integrin αvβ3 binding site became more flexible and the corresponding calcium ions were shed from the binding site. The strongest interaction force was exhibited at the binding site of the integrin β3 subunit at 310 K while it was found at the binding site of the integrin αv subunit at higher temperatures, owing to the smaller structure deformation of the integrin αv subunit.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48178199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective cell penetration of negatively‐charged proline‐rich SAP(E) peptides with cysteine mutation 半胱氨酸突变的带负电荷富含脯氨酸的SAP（E）肽的有效细胞穿透

IF 2.4 4区生物学

Peptide Science Pub Date : 2022-12-09 DOI: 10.1002/pep2.24301

Sewon Lim, Jinhyuk Park, Seung‐Eun Chong, Sungwhan Kim, Yoonhwa Choi, Sohee Nam, Yan Lee

{"title":"Effective cell penetration of negatively‐charged proline‐rich SAP(E) peptides with cysteine mutation","authors":"Sewon Lim, Jinhyuk Park, Seung‐Eun Chong, Sungwhan Kim, Yoonhwa Choi, Sohee Nam, Yan Lee","doi":"10.1002/pep2.24301","DOIUrl":"https://doi.org/10.1002/pep2.24301","url":null,"abstract":"Most of cell penetrating peptides (CPPs) are rich in positively‐charged amino acids but the cationic property may provoke possible problems in practical applications. In this study, we carefully substituted the hydrophobic amino acids in the SAP(E) sequence, a rare example of negatively‐charged proline‐rich CPP, with cysteine for enhancement of cell penetrating activity as well as reversible conjugation of cargo molecules. Most substituents showed almost negligible cell penetrating activity, but a cysteine substituent on the 7th valine (SAP(E)‐7C) showed more improved cell penetrating activity than SAP(E). When treated to cells, the negatively‐charged SAP(E)‐7C exhibited much lower degree of co‐localization with acidic endosomes or lysosomes compared to positively‐charged TAT. SAP(E)‐7C could significantly enhance the PTX efficacy on MDA‐MB‐231 cells by non‐covalent complexation with PTX. As a proof‐of‐concept for covalent conjugation of cargo drugs, mercaptoethanol, a model drug, was conjugated to the cysteine residue of SAP(E)‐7C via a disulfide bond, and the glutathione‐dependent release from the conjugate was confirmed. The negatively‐charged SAP(E)‐7C with a cysteine handle can be a useful molecular module for the development of CPP‐based drug delivery carrier.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49375714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information 问题信息

IF 2.4 4区生物学

Peptide Science Pub Date : 2022-11-01 DOI: 10.1002/pep2.24300

引用次数: 0

Mutagenesis of cyclotide Cter 27 exemplifies a robust folding strategy for bracelet cyclotides 环核苷酸Cter 27的突变是环核苷酸链折叠策略的一个例子

IF 2.4 4区生物学

Peptide Science Pub Date : 2022-11-01 DOI: 10.1002/pep2.24284

Tien T. Dang, P. Harvey, L. Y. Chan, Yen‐Hua Huang, Q. Kaas, D. Craik

引用次数: 2

PL‐101‐WK, a novel tryptophan‐ and lysine‐rich peptide with antimicrobial activity against Staphylococcus aureus PL‐101‐WK，一种新型富含色氨酸和赖氨酸的肽，对金黄色葡萄球菌具有抗菌活性

IF 2.4 4区生物学

Peptide Science Pub Date : 2022-10-05 DOI: 10.1002/pep2.24296

Parvin Zadeh Shahraki, P. Farrokh

{"title":"PL‐101‐WK, a novel tryptophan‐ and lysine‐rich peptide with antimicrobial activity against Staphylococcus aureus","authors":"Parvin Zadeh Shahraki, P. Farrokh","doi":"10.1002/pep2.24296","DOIUrl":"https://doi.org/10.1002/pep2.24296","url":null,"abstract":"Designing short antimicrobial peptides (AMPs), which are active against drug‐resistant bacteria, is a promising way to find new therapeutic agents. In this research, a novel short AMP, PL‐101‐WK, was designed based on PL‐101 (a derivative of plicatamide). Here, the substitution of Phe and His with Trp and Lys was considered. The antimicrobial activity and physicochemical properties of PL‐101‐WK were compared with PL‐101 by in silico analysis. The antimicrobial activity in the presence or absence of NaCl concentration, thermal stability, hemolytic activity, and selectivity of the peptides were determined. By substitution of Lys and Trp residues, positive charge, in vitro stability, and hydrophilicity of PL‐101‐WK were raised compared to the template. PL‐101‐WK had the best minimum inhibitory concentration (MIC) value of 64 μg/ml against Staphylococcus aureus strains, which showed at least 16‐fold reduction when compared to the values of PL‐101. The MICs of PL‐101‐WK were retained toward S. aureus strains at physiological salt concentration. While PL‐101‐WK did not display acceptable thermal stability, it had desirable selectivity against bacteria. The maximum hemolytic activity of PL‐101‐WK was 1.65% at 512 μg/ml. Taken together, increasing positive charge and the presence of Trp residues were enhanced the potential of antibacterial activity of PL‐101.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2022-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46542752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Shelf Stable Fmoc Hydrazine Resin for the Synthesis of Peptide Hydrazides. 用于合成肽肼的货架稳定的 Fmoc 肼树脂。

IF 1.5 4区生物学

Peptide Science Pub Date : 2022-09-01 Epub Date: 2022-04-16 DOI: 10.1002/pep2.24268

Michael J Bird, Philip E Dawson

引用次数: 0

Linearized teixobactin is inactive and after sequence enhancement, kills methicillin-resistant Staphylococcus aureus via a different mechanism. 线性化的 teixobactin 没有活性，经过序列增强后，可通过不同的机制杀死耐甲氧西林金黄色葡萄球菌。

IF 1.5 4区生物学

Peptide Science Pub Date : 2022-09-01 Epub Date: 2022-04-25 DOI: 10.1002/pep2.24269

Qianhui Wu, Biswajit Mishra, Guangshun Wang

{"title":"Linearized teixobactin is inactive and after sequence enhancement, kills methicillin-resistant Staphylococcus aureus via a different mechanism.","authors":"Qianhui Wu, Biswajit Mishra, Guangshun Wang","doi":"10.1002/pep2.24269","DOIUrl":"10.1002/pep2.24269","url":null,"abstract":"Staphylococcus aureus is a highly adaptable pathogen that can rapidly develop resistance to conventional antibiotics such as penicillin. Recently, teixobactin was discovered from uncultivated soil bacteria by using the i-chip technology. This depsipeptide forms an ester bond between the backbone C-terminal isoleucine carboxylic acid and the hydroxyl group of threonine at position 8. Also, it contains multiple nonstandard amino acids, making it costly to synthesize. This study reports new peptides designed by linearizing teixobactin. After linearization and conversion to normal amino acids, teixobactin lost its antibacterial activity. Using this inactive template, a series of peptides were designed via hydrophobic patching and residue replacements. Three out of the five peptides were active. YZ105, only active against Gram-positive bacteria, however, showed the highest cell selectivity index. Different from teixobactin, which inhibits cell wall synthesis, YZ105 targeted the membranes of methicillin-resistant S. aureus (MRSA) based on kinetic killing, membrane permeation, depolarization, and scanning electron microscopy studies. Moreover, YZ105 could kill nafcillin-resistant MRSA, Staphylococcal clinical strains, and disrupted preformed biofilms. Taken together, YZ105, with a simpler sequence, is a promising lead for developing novel anti-MRSA agents.","PeriodicalId":19825,"journal":{"name":"Peptide Science","volume":"114 5","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/64/fd/PEP2-114-e24269.PMC9564113.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10452235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structured cyclic peptide mimics by chemical ligation 化学连接的结构环肽模拟物

IF 2.4 4区生物学

Peptide Science Pub Date : 2022-09-01 DOI: 10.1002/pep2.24266

B. C. Atkinson, A. Thomson

引用次数: 0