Pakistan Journal of Biological Sciences最新文献

{"title":"Antibacterial Activity of <i>Curcuma mangga</i> Extracts Against Antibiotic-Resistant Bacteria: Natural Alternative for Combating Multidrug Resistance.","authors":"Nonthanan Phonphan, Waranan Phonphan, Kawinthida Punyavong, Krittaphat Pumipuntu, Ekkwit Chaichamnong, Surachai Rattanasuk","doi":"10.3923/pjbs.2025.403.409","DOIUrl":"10.3923/pjbs.2025.403.409","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; &lt;i&gt;Curcuma mangga&lt;/i&gt;, commonly known as mango ginger, is a medicinal plant renowned for its anti-inflammatory, antioxidant and digestive health benefits, attributed to its high content of curcuminoids and essential oils. This study aims to assess the antibacterial potential of &lt;i&gt;C. mangga&lt;/i&gt; extracts against five antibiotic-resistant bacterial strains and five standard pathogenic reference strains. &lt;b&gt;Materials and Methods:&lt;/b&gt; &lt;i&gt;C. mangga&lt;/i&gt; rhizomes were dried, ground into powder and individually extracted using ethanol, dichloromethane and hexane. The antibacterial activity was assessed using the microbroth dilution method to find the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Statistical analysis was performed using Duncan's Multiple Range Test (DMRT) to show significant differences among mean values at a 95% confidence level. &lt;b&gt;Results:&lt;/b&gt; The dichloromethane and hexane extracts exhibited the strongest inhibitory effect, showing the lowest MIC value of 0.049 mg/mL against multidrug-resistant &lt;i&gt;Klebsiella pneumoniae&lt;/i&gt; and &lt;i&gt;Stenotrophomonas maltophilia&lt;/i&gt;. The dichloromethane extract showed the strongest bactericidal effect against &lt;i&gt;S. maltophilia&lt;/i&gt;, with the lowest MBC value of 1.56 mg/mL. &lt;b&gt;Conclusion:&lt;/b&gt; This study is the first to prove that &lt;i&gt;C. mangga&lt;/i&gt; extracts exhibit significant antibacterial and bactericidal activity against several antibiotic-resistant pathogens, including multidrug-resistant &lt;i&gt;K. pneumoniae&lt;/i&gt; and &lt;i&gt;S. maltophilia&lt;/i&gt;. These findings highlight the potential of &lt;i&gt;C. mangga&lt;/i&gt; as a natural antimicrobial agent and lay the groundwork for the development of plant-based alternatives to address the growing threat of antibiotic resistance.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 6","pages":"403-409"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnopharmacology and Therapeutic Value of <i>Clematis brachiata </i>Thunb. (Family: Ranunculaceae): A Narrative Review.","authors":"Alfred Maroyi","doi":"10.3923/pjbs.2025.359.368","DOIUrl":"10.3923/pjbs.2025.359.368","url":null,"abstract":"<p><p>&lt;i&gt;Clematis brachiata&lt;/i&gt; Thunb. is a widely recognized medicinal plant commonly utilized across tropical Africa. This review consolidates information on the medicinal applications and pharmacological activities of &lt;i&gt;C. brachiata&lt;/i&gt;. Data regarding its traditional uses and ethnopharmacological significance were sourced from various platforms, including Google Scholar, Web of Science, ScienceDirect&lt;sup&gt;®&lt;/sup&gt;, Scopus&lt;sup&gt;®&lt;/sup&gt;, SpringerLink&lt;sup&gt;®&lt;/sup&gt;, PubMed&lt;sup&gt;®&lt;/sup&gt; and SciELO, as well as older printed literature available in the university library. The findings highlight the use of different parts of &lt;i&gt;C. brachiata&lt;/i&gt;, such as branches, flowers, leaf juice, leaves, root bark, roots, shoots, stem bark, stems and the entire plant, for various purposes. These include serving as a vermifuge, in charms and rituals and traditional remedies for conditions like back pain, eye disorders, fever, febrile illnesses, gastrointestinal issues, headaches, malaria, respiratory problems, skin infections, snakebites, sexually transmitted infections and sores. Chemical compounds identified from &lt;i&gt;C. brachiata&lt;/i&gt; include tannins, saponins, flavonoids, terpenoids, steroids and cardiac glycosides. The crude extracts of &lt;i&gt;C. brachiata&lt;/i&gt; flowers, leaves, roots, root bark, stems and whole plants exhibited acaricidal, analgesic, antihelmintic, antibacterial, antifungal, anti-inflammatory, antileishmanial, antioxidant, antiplasmodial and antipyretic activities. Future studies should focus on detailed ethnopharmacological evaluation of &lt;i&gt;C. brachiata&lt;/i&gt;, particularly its phytochemistry, pharmacological properties and toxicological evaluations, &lt;i&gt;in vivo&lt;/i&gt; and clinical research.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 6","pages":"359-368"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Chelated Copper, Selenium and Zinc on the Productive Performance of Growing V-Line Rabbits.","authors":"Asmaa Mahmoud Hamed Abd El-Hamed Ashour, Abdallah Ali Ghazalah, Mamdouh Omar Abd El-Samee, Adel Eid Mohamed Mahmoud","doi":"10.3923/pjbs.2025.392.402","DOIUrl":"10.3923/pjbs.2025.392.402","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Chelated minerals have been shown to enhance animal performance and increase feed consumption, improving feed utilization. So, the present study aimed to determine the effect of adding each zinc at 50 mg, copper at 3 mg and selenium at 0.2 mg, all in chelated form, to the diets on growth performance, digestibility, meat quality, immune response, carcass traits, blood biochemical characteristics and economic efficiency of rabbits. &lt;b&gt;Materials and Methods:&lt;/b&gt; In this experiment, 60 V-Line strain rabbits of mixed sex with an average body weight of 834 g were randomly and individually distributed into five experimental treatments (12 rabbits per treatment). The study included 5 groups, each comprising 12 rabbits. The first group (C) served as the control and received the basal diet without supplements. The second and third groups were given the basal diet supplemented with 50 mg zinc and 3 mg copper, respectively. The fourth group received the basal diet with 0.2 mg of selenium, while the fifth group was supplemented with a combination of zinc, copper and selenium. Data were analyzed using One-way ANOVA (GLM, SAS) and treatment means were compared by Duncan's test at p<0.05. &lt;b&gt;Results:&lt;/b&gt; Rabbits fed diets supplemented with the tested mineral additives showed significant improvements in feed conversion ratio, final body weight and weight gain compared to the control group. These groups also exhibited higher nutrient digestibility, as indicated by dry matter (DM), crude protein (CP), crude fiber (CF), ether extract (EE) and nitrogen-free extract (NFE). However, giblets yield (%) was significantly higher in the control group, whereas dressing percentage followed an opposite trend. Additionally, rabbits receiving mineral-supplemented diets demonstrated better economic efficiency than those on the control diet. &lt;b&gt;Conclusion:&lt;/b&gt; That supplementing growing rabbit diets with zinc (50 mg), copper (3 mg) and selenium (0.2 mg) significantly enhanced body weight gain, feed conversion ratio, immune response and economic efficiency over an 8-week growing period. These trace elements act as effective growth promoters, likely due to their digestive-enhancing, antimicrobial and performance-boosting properties. The findings confirm that zinc, copper and selenium positively influence both productivity and economic outcomes in growing rabbits.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 6","pages":"392-402"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Therapeutic Effect of Vitamin C on Methotrexate- Induced Damage in the Cerebral Cortex.","authors":"Ali Hassan Abdou Ali, Amany Mostafa Abo-Ouf, Heba Abdelnaser Aboelsoud, Mohammed Nawaf Alharbi, Aryaf Mohammed Almutairi, Abdullah Fahad Aljarboa, Nasser Ibrahim Alshumaymiri, Abdallah Saleh Alayyaf, Abdulaziz Muidh Alshamrani, Abdullah Taysir Alhaddad, Dhafer Mana Alamri, Abdullah Mohammed Alqahtani, Salem Abdulhadi Aldosari","doi":"10.3923/pjbs.2025.8.15","DOIUrl":"10.3923/pjbs.2025.8.15","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Methotrexate is an anti-metabolic medication used to treat cancer. It causes oxidative stress in nerve tissue and has neurotoxic effects. A strong antioxidant and effective free radical scavenger is vitamin C. The current research aims to investigate the potential protective impact of vitamin C and the toxic consequences of methotrexate. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Thirty-six rats were used in this research and group one (Group 1) got no treatment at all. For 4 weeks, (Group 2) underwent a single intraperitoneal injection of methotrexate at a dose of 20 mg/kg once a week and (Group 3) got methotrexate at the same dosage as Group 2 and vitamin C (20 mg/kg) intragastrically every other day for four weeks. Rats were killed after the experiment and brain hemispheres were removed and prepared for light microscopic analysis. The cerebral hemispheres were ready for biochemical analysis to determine the brain tissue's concentrations of MDA, CAT, GSH and SOD. Data analysis was conducted using SPSS software version 20. &lt;b&gt;Results:&lt;/b&gt; In the methotrexate (2)-treated group, there were histological alterations manifested as a reduction in granular layer thickness. Purkinje cells exhibit a reduction in number, a shrinking of the cell bodies and a loss of monolaminar organization. Reduced cellularity was seen in the molecular layer. These cellular alterations are lessened and the thickness of the granular and molecular cell layers is restored following vitamin C treatment. When compared to the MTX+Vitamin C group, vitamin C greatly attenuates the biochemical and histological alterations caused by MTX. &lt;b&gt;Conclusion:&lt;/b&gt; Results concluded that although methotrexate is a toxic medication that damages the brain cortex, its toxicity is reduced when vitamin C is taken with it.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological Responses to Salinity Stress at the Germination Phase in Eight Rice (<i>Oryza sativa</i> L.) Cultivars Cultivated in North Sulawesi, Indonesia.","authors":"Song Ai Nio, Daniel Peter Mantilen Ludong, Ratna Siahaan","doi":"10.3923/pjbs.2025.70.77","DOIUrl":"10.3923/pjbs.2025.70.77","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; The biodiversity of rice cultivars, including local rice from North Sulawesi, represents a potential source of germplasm for fulfilling national food needs. A few publications related to the characteristics of salinity stress resistance in rice cultivars, including local rice from North Sulawesi. This study aimed to examine the morphological response to salinity stress at the germination phase in eight rice cultivars cultivated in North Sulawesi, Indonesia. &lt;b&gt;Materials and Methods:&lt;/b&gt; The randomized block design experiment was conducted in the laboratory using eight rice cultivars (Superwin, Ombong, Temo, Burungan, Ciherang, Inpari 31, Inpago 10 and TB) using the Roll Paper Test method during the germination phase. The treatments in this experiment were salinity stress of 0, 10, 20 and 30% seawater for 14 days. The morphological characteristics evaluated as a response to salinity stress consisted of germination percentage, normal germination percentage, root length, shoot length and root-to-shoot ratio. To determine the significant differences and interactions between treatments (where, p<0.05), the data were analyzed using Analysis of Variance (ANOVA) followed by Least Significant Difference (LSD) 5%. &lt;b&gt;Results:&lt;/b&gt; Salinity stress reduced germination rates, including overall and normal germination and shorter shoot and root lengths. However, this stress caused an increase in the root-to-shoot ratio. The Roll Paper Test with salinity treatment at the germination phase could be used as a simple selection method to obtain salinity-tolerant-rice cultivars. &lt;b&gt;Conclusion:&lt;/b&gt; Shoot length and root-to-shoot ratio were potential morphological indicators for salinity tolerance in rice.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 2","pages":"70-77"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methamphetamine Neurotoxicity: Neurotoxic Effects, Mechanism of Toxicity, Molecular Mechanisms and Treatment Strategies.","authors":"Yahya Saleh Al-Awthan, Mohamed Khalid Al-Homidi, Abdulrahman Rashid Albalawi, Khalil Ibrahim Almousi","doi":"10.3923/pjbs.2024.613.625","DOIUrl":"https://doi.org/10.3923/pjbs.2024.613.625","url":null,"abstract":"<p><p>Methamphetamine (METH) is a highly addictive and dangerous drug that mainly affects neurotransmitters in the brain and leads to feelings of alertness and euphoria. The METH use can lead to addiction, which has become a worldwide problem, resulting in a slew of public health and safety issues. Recent studies showed that chronic METH use can lead to neurotoxicity, neuro-inflammation and oxidative stress which can lead to neuronal injury. This review discussed the history of METH use, the link between METH use and neurotoxicity, the molecular mechanism and the different treatment strategies. This study attempted to discuss some of the drug's principal impacts and gave proof in favor of a few of the cellular and molecular causes of METH neurotoxicity. In addition, it demonstrates the most recent treatment strategies involving mitigating METH-induced neurotoxicity. However, future studies are needed to better understand the mechanism by which METH use induced neurotoxicity.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"613-625"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomy, Traditional Uses and Pharmacological Properties of <i>Ormocarpum trichocarpum</i> (Taub.) Engl. (Fabaceae Family): A Narrative Review.","authors":"Alfred Maroyi","doi":"10.3923/pjbs.2024.626.634","DOIUrl":"https://doi.org/10.3923/pjbs.2024.626.634","url":null,"abstract":"<p><p>&lt;i&gt;Ormocarpum trichocarpum&lt;/i&gt; (Taub.) Engl. is a shrub or small tree harvested from the wild as a source of food, traditional medicines and wood. The present review compiles existing information on the medicinal uses and chemical and pharmacological properties of &lt;i&gt;O. trichocarpum&lt;/i&gt;. Multiple searches on existing literature on the traditional, medicinal, phytochemistry and pharmacological properties of &lt;i&gt;O. trichocarpum&lt;/i&gt; were conducted in online Databases such as Web of Science, Google Scholar, Scopus&lt;sup&gt;®&lt;/sup&gt;, SpringerLink&lt;sup&gt;®&lt;/sup&gt;, ScienceDirect&lt;sup&gt;®&lt;/sup&gt;, SciELO and PubMed&lt;sup&gt;®&lt;/sup&gt;, as well as using pre-electronic literature sources obtained from the university library. This study showed that the bark, leaves, roots, stem bark, stems and whole plant parts of &lt;i&gt;O. trichocarpum&lt;/i&gt; are used against 32 human and animal diseases and ailments.&lt;i&gt; &lt;/i&gt;The main ailments and diseases treated by &lt;i&gt;O. trichocarpum&lt;/i&gt; crude extracts include its use as an emetic, poisoning antidote, protective charm and traditional medicine for erectile dysfunction, gastrointestinal problems, prolonged labour, sexually transmitted infections and skin infections. The phytochemical evaluation of the plant revealed that it contains alkaloids, alkanes, flavonoids, phenolics, saponins, tannins, diterpenes, sesquiterpenes and bioflavonoids. Pharmacological assessments showed that the crude extracts and phytochemical compounds isolated from the species have antibacterial, antifungal, antioxidant, antiplasmodial and tyrosinase-inhibiting activities. Detailed studies focusing on toxicological evaluations, &lt;i&gt;in vivo&lt;/i&gt; and clinical research aimed at corroborating the traditional medical applications of &lt;i&gt;O. trichocarpum&lt;/i&gt; are recommended.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"626-634"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Biosaka Elicitor Concentration for Enhanced Growth and Yield of Cherry Tomato (<i>Solanum lycopersicum</i> L. var. <i>cerasiforme</i>).","authors":"Elimasni Elimasni, Sayyidah Afinah Salsabila Nasution","doi":"10.3923/pjbs.2024.577.586","DOIUrl":"https://doi.org/10.3923/pjbs.2024.577.586","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Prolonged utilization of chemical fertilizers can harm the soil and disturb the equilibrium of nutrients, resulting in a decline in cherry tomato yield. To enhance the growth of cherry tomato plants, it is necessary to add organic chemicals. The research aimed to determine the best elicitor biosaka concentration to apply to evoke the plant growth of cherry tomatoes (&lt;i&gt;Solanum lycopersicum&lt;/i&gt; L. var. &lt;i&gt;cerasiforme&lt;/i&gt;). &lt;b&gt;Materials and Methods:&lt;/b&gt; This study employed a complete random design with four treatments of biosaka formula: B0 (control, water), B1 (1.5 mL/L), B2 (2.5 mL/L), B3 (3.5 mL/L) and B4 (4.5 mL/L). Twenty-day-old tomato seedlings were transplanted into polybags filled with a compost and husk charcoal mixture. Biosaka application was done every four days by spraying at a 0.5 m distance, starting from the first day after transplanting. Lycopene levels were measured using a UV-vis spectrophotometer at 417 nm after a series of solvent extractions. Data were analyzed using ANOVA for significant effects. &lt;b&gt;Results:&lt;/b&gt; The biosaka concentration of 4.5 mL/L gives the highest results for the variables of treatments such as the plant height is up to 167.8 cm, the number of petioles is 62.8 petioles, the number of fruit is 19.6 and the age at flowering is 44.4 days. The biosaka concentration of 4.5 mL/L gave the highest yield for the variable plant height of 167.8 cm, the number of petioles 62.8, the number of fruits per plant 19.6, the initial flowering age of 44.4 days, the wet weight of the plant was 740 g and the dry weight of the plant was 125.1 g. &lt;b&gt;Conclusion:&lt;/b&gt; The optimal concentration of biosaka (4.5 mL/L) significantly improved cherry tomato growth and yield, resulting in increased plant height, number of petioles and fruit count. It also accelerated the initial flowering age and enhanced both wet and dry plant weights. This indicates that 4.5 mL/L of biosaka effectively promotes the growth and productivity of cherry tomato plants.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"577-586"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effects of Turmeric, Strawberry and Broccoli in Improving Lipid Profile in Adult Patients with Hypercholesterolemia.","authors":"Diana Krisanti Jasaputra, Theresia Monica Rahardjo, Julia Windi Gunadi, Shiela Stefani, Ivana Indriati Sutrisno","doi":"10.3923/pjbs.2024.587.593","DOIUrl":"https://doi.org/10.3923/pjbs.2024.587.593","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Turmeric, strawberries and broccoli are popular in the community for their beneficial effects in improving lipid profile, but poor bioavailability and absorption of their phytochemical compounds might reduce their effects while given separately. Therefore, their combination might provide a synergistic enhancement of their property as hypolipidemic agents. This study aims to examine the effects of turmeric, strawberry and broccoli in improving lipid profile in adult patients with hypercholesterolemia. &lt;b&gt;Materials and Methods:&lt;/b&gt; Twenty hypercholesterolemic adult patients from Unggul Karsa Medika Hospital in Bandung, Indonesia were recruited for the study, they were given 600 mg/day of turmeric/curcuminoid, 100 g of fresh strawberry and 100 g of boiled broccoli for 30 days. The measurement of the patient's characteristics (body weight, height, BMI) and biochemical parameters in the blood (total cholesterol, triglyceride, LDL-C, HDL-C, SGOT, SGPT, urea and creatinine) were conducted before and after the treatment. &lt;b&gt;Results:&lt;/b&gt; The mean age of the patients was 49.15±1.9 years old, with mean height 1.56±0.014 m, weight 61.43±1.96 kg and mean body mass index 25.25±0.87 kg/m&lt;sup&gt;&lt;a href=\"86677_op\"&gt;2&lt;/a&gt;&lt;/sup&gt;. Lipid profile before the treatment: Total cholesterol 239.75±6.56 mg/dL, LDL-C 172.9±6.53 mg/dL, HDL-C 66.55±3.37 mg/dL and triglyceride 193.7±18.45 mg/dL; after the treatment: Total cholesterol 220.7±9.39 mg/dL, LDL-C 153.95±9.98 mg/dL, HDL-C 59.2±2.45 mg/dL and triglyceride 145±16.55 mg/dL. The result showed a significant reduction of total cholesterol (p = 0.014), LDL-C (p = 0.036), HDL-C (p = 0.001) and triglyceride (p = 0.015) after the treatment. Other biochemical parameters (SGOT, SGPT, urea and creatinine) showed normal levels before and after the treatment. &lt;b&gt;Conclusion:&lt;/b&gt; In summary, supplementation of turmeric, strawberry and broccoli improves lipid profile in adult patients with hypercholesterolemia.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"587-593"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Alterations of Cervical Cancer Following <i>Zanthoxylum acanthopodium</i> DC Therapy in Relation to E7, pRb, EGFR and p16 Expression.","authors":"Rostime Hermayerni Simanullang, Jekson Martiar Siahaan, Putri Cahaya Situmorang","doi":"10.3923/pjbs.2024.602.612","DOIUrl":"https://doi.org/10.3923/pjbs.2024.602.612","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Cervical cancer is the second most common cancer in Indonesia, where traditional herbal treatments like &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; (andaliman) are culturally used. Investigating protein biomarkers such as E7, pRb, EGFR and p16 can help assess the efficacy of these treatments. &lt;b&gt;Materials and Methods:&lt;/b&gt; There were 5 groups in this study: 2 control groups (C- and C+) and 3 treatment groups (each receiving one of three doses). Oral administration of andaliman was performed for 30 days in cancer model rats, after which the cervix was dissected, cervical tissue was taken and immunohistochemistry repair was performed. Statistical analysis was performed using the Kruskal-Wallis test with a p<0.05. &lt;b&gt;Results:&lt;/b&gt; As &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; DC dose rose, cervical tissue E7, EGFR and p16 expression decreased. However, greater doses of this plant increased cervical tissue pRb protein. Cervical cancer histology exhibited increased nuclear size, irregular cellular structure, atypical cell shape, higher nuclear-cytoplasmic ratio and various nuclear shape variants. This herb induced tissue to show well-organized non-hyperchromatic cells that resembled normal clusters. &lt;b&gt;Conclusion:&lt;/b&gt; &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; DC improved cervical tissue and balanced cervical cancer biomarker proteins such E7, EGFR, pRB and p16.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"602-612"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}