Pakistan Journal of Biological Sciences最新文献

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Survey of Physicochemical Variables in Molepo Dam, South Africa, Using Multivariate Analysis. 利用多变量分析法调查南非莫莱波大坝的物理化学变量。
Pakistan Journal of Biological Sciences Pub Date : 2024-03-01 DOI: 10.3923/pjbs.2024.219.223
Gavin Geldenhuys
{"title":"Survey of Physicochemical Variables in Molepo Dam, South Africa, Using Multivariate Analysis.","authors":"Gavin Geldenhuys","doi":"10.3923/pjbs.2024.219.223","DOIUrl":"https://doi.org/10.3923/pjbs.2024.219.223","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Molepo Dam is a small dam with several aquatic animal species. An assessment of the water quality index of Molepo Dam is necessary because it is situated close to a largely rural community. In this study, the physicochemical variables of Molepo Dam were observed to better understand the water quality situation of this dam. &lt;b&gt;Materials and Methods:&lt;/b&gt; The study was carried out from October 2022 to March 2023; 126 samples of water were taken from the Molepo Dam. The physicochemical parameters of the water were determined through standard methods. &lt;b&gt;Results:&lt;/b&gt; The site within the dam exhibited a clear separation. Site 1, 2 and 4 were found to be more similar to each other, while Site 3 was separated from them. Site 5, 6 and 7 were more similar together as well. The results showed that the pollution level in Site 3 was the lowest. The pollution level in Site 1, 2 and 4 was more similar and the level of pollution in Site 5, 6 and 7 was the highest. &lt;b&gt;Conclusion:&lt;/b&gt; According to the study, pollution levels were found to be different in various parts of the Molepo Dam. This was because the wastewater generated by rural communities was predominantly discharged towards the northern part of the dam.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141173811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GC-MS and ATR-FTIR Spectroscopy Coupled with Chemometric Analysis for Detection and Quantification of White Turmeric (Curcuma zedoaria) Essential Oils Adulteration. GC-MS 和 ATR-FTIR 光谱法与化学计量学分析相结合用于检测和定量白姜黄(Curcuma zedoaria)精油掺假。
Pakistan Journal of Biological Sciences Pub Date : 2024-03-01 DOI: 10.3923/pjbs.2024.160.167
Suryati Syafri, Ghada Nabila Gari Lindo, Yohannes Alen, Syofyan Syofyan, Dachriyanus Hamidi
{"title":"GC-MS and ATR-FTIR Spectroscopy Coupled with Chemometric Analysis for Detection and Quantification of White Turmeric (<i>Curcuma zedoaria</i>) Essential Oils Adulteration.","authors":"Suryati Syafri, Ghada Nabila Gari Lindo, Yohannes Alen, Syofyan Syofyan, Dachriyanus Hamidi","doi":"10.3923/pjbs.2024.160.167","DOIUrl":"https://doi.org/10.3923/pjbs.2024.160.167","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; White turmeric essential oil (WTEO) is known to have high commercial value since it has been used to improve immunological function, increase blood circulation, ease toxin clearance and stimulate digestion. However, there is no standard to regulate the specific characteristics of white turmeric essential oil. Therefore, the objective of this research was to develop an analytical technique for WTEO authentication from vegetable oils, namely palm oil (PO), coconut oil (VCO) and soybean oil (SO), using FTIR spectroscopy and chemometrics, as well as GC-MS spectroscopy. &lt;b&gt;Materials and Methods:&lt;/b&gt; The WTEO was obtained by hydrodistillation method. Pure WTEO and vegetable oils were scanned in the MIR region (4000-650 cm&lt;sup&gt;1&lt;/sup&gt;) of FTIR spectroscopy and the spectra were further analyzed using chemometrics. &lt;b&gt;Results:&lt;/b&gt; The extraction yielded 0.103% v/w WTEO, a dark purple color with a specific pungent odor. Discriminant analysis separated pure WTEO and adulterated WTEO with 100% accuracy at wave numbers 4000-650 cm&lt;sup&gt;1&lt;/sup&gt;. The best PLS regressions to quantify SO, VCO, PO and concentration in WTEO were at wave numbers 4000-1100, 1400-1050 and 2100-650 cm&lt;sup&gt;1&lt;/sup&gt;, respectively. &lt;b&gt;Conclusion:&lt;/b&gt; The FTIR and chemometrics combination effectively authenticates white turmeric essential oil from any possible adulterants, such as vegetable oil.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140861134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effectiveness of Gel from Andaliman Fruit (Zanthoxylum acanthopodium DC.) Extract on Wound Inflammation. 安达利曼果(Zanthoxylum acanthopodium DC.)
Pakistan Journal of Biological Sciences Pub Date : 2024-03-01 DOI: 10.3923/pjbs.2024.142.151
Elsa Izza Sabila, Annisa Ayu Ramadhani, Hanif Fadhilah, Balqis Nur Nasution, Fathiya, Putri Cahaya Situmorang
{"title":"Effectiveness of Gel from Andaliman Fruit (<i>Zanthoxylum acanthopodium</i> DC.) Extract on Wound Inflammation.","authors":"Elsa Izza Sabila, Annisa Ayu Ramadhani, Hanif Fadhilah, Balqis Nur Nasution, Fathiya, Putri Cahaya Situmorang","doi":"10.3923/pjbs.2024.142.151","DOIUrl":"https://doi.org/10.3923/pjbs.2024.142.151","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Pain is caused by damaged tissue on the outside or inside of an organ and it is regulated by chemicals. Synthetic drugs are used to ease pain because they are analgesics in the field of medicine. Traditional medicine is known to help people all over the world, in both rich and developing nations. The Andaliman fruit, or &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; DC., comes from a spice plant that grows naturally in Toba Regency, North Sumatra, Indonesia. This study aims to determine the formulation of the nanoherbal analgesic spray gel preparation of Andaliman fruit (&lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; DC.) in terms of its effectiveness as a pain reliever. &lt;b&gt;Materials and Methods:&lt;/b&gt; There were three amounts of spray gel made, namely 5, 10 and 15% and tests were done to see how well they worked. &lt;i&gt;Staphylococcus aureus&lt;/i&gt; bacteria were used in the bacterial blocking test. To test how well painkillers worked, five groups of mice were used. Using a hot iron, tests for anti-inflammatory activity and wound healing were done. The tissue was then watched for 14 days and analysed using Hematoxylin and Eosin (H&E) stains. &lt;b&gt;Results:&lt;/b&gt; The 15% concentration reduces pain and the time it takes for the body to respond to it. The clear zone size is the same as (K&lt;sup&gt;+&lt;/sup&gt;) and it can lower the number of inflammatory cells and help wounds heal by adding fibroblast and collagen cells. These findings are supported by significant data results (p<0.05, p = 0.018). &lt;b&gt;Conclusion:&lt;/b&gt; Finally, analgesic gel spray made from the Andaliman fruit at a 15% concentration can help with pain and also be antibacterial, reduce inflammation and help wounds heal.&lt;/p&gt.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140865076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant and Antibacterial Properties of 1,3-Dihydroxy-,2', 2'-Dimethylpyrano-(5,6)-Xanthone from Streptomyces sp. SU84 来自链霉菌 SU84 的 1,3-二羟基-,2',2'-二甲基吡喃-(5,6)-氧杂蒽酮的抗氧化和抗菌特性
Pakistan Journal of Biological Sciences Pub Date : 2024-02-15 DOI: 10.3923/pjbs.2024.132.141
Thongchai Taechowisa, T. Chuen-Im, Waya S. Phutdhawon
{"title":"Antioxidant and Antibacterial Properties of 1,3-Dihydroxy-,2', 2'-Dimethylpyrano-(5,6)-Xanthone from Streptomyces sp. SU84","authors":"Thongchai Taechowisa, T. Chuen-Im, Waya S. Phutdhawon","doi":"10.3923/pjbs.2024.132.141","DOIUrl":"https://doi.org/10.3923/pjbs.2024.132.141","url":null,"abstract":"","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140455190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Doxorubicin, doxorubicinol, cardiotoxicity, breast cancer, volumetric absorptive microsampling, LC-MS/MS 多柔比星、多柔比星醇、心脏毒性、乳腺癌、体积吸收微取样、LC-MS/MS
Pakistan Journal of Biological Sciences Pub Date : 2024-02-15 DOI: 10.3923/pjbs.2024.125.131
Dian Fitri Chairunnis, Y. Harahap, Maria Juanita, N. F. Syafhan, D. Purwanto
{"title":"Doxorubicin, doxorubicinol, cardiotoxicity, breast cancer, volumetric absorptive microsampling, LC-MS/MS","authors":"Dian Fitri Chairunnis, Y. Harahap, Maria Juanita, N. F. Syafhan, D. Purwanto","doi":"10.3923/pjbs.2024.125.131","DOIUrl":"https://doi.org/10.3923/pjbs.2024.125.131","url":null,"abstract":"","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140456052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antioxidant Activities of Stem, Leaves and Fruits Extracts of Pepino (Solanum muricatum Aiton). 佩皮诺(Solanum muricatum Aiton)茎、叶和果实提取物的抗氧化活性。
Pakistan Journal of Biological Sciences Pub Date : 2024-01-01 DOI: 10.3923/pjbs.2024.69.79
Rika Hartati, Nethania Alysia Febiana, Hegar Pramastya, Irda Fidrianny
{"title":"Antioxidant Activities of Stem, Leaves and Fruits Extracts of Pepino (<i>Solanum muricatum</i> Aiton).","authors":"Rika Hartati, Nethania Alysia Febiana, Hegar Pramastya, Irda Fidrianny","doi":"10.3923/pjbs.2024.69.79","DOIUrl":"10.3923/pjbs.2024.69.79","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Pepino (&lt;i&gt;Solanum muricatum&lt;/i&gt; Aiton), rich with vitamin C and flavonoids, constitutes an abundant source of potent antioxidants. This research was conducted to determine antioxidant activity from three different parts of pepino based on equivalence with ascorbic acid, to analyze the relationship between total phenolic content (TPC) and total flavonoid content (TFC) on antioxidant activities and to determine flavonoid compounds. &lt;b&gt;Materials and Methods:&lt;/b&gt; Antioxidant activities were determined using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and Cupric Ion Reducing Antioxidant Capacity (CUPRAC) methods. The TPC and TFC were determined by UV-visible spectrophotometry. The correlation between TPC, TFC and antioxidant activity was analyzed using Pearson's method. Flavonoid compound content was performed by HPLC. &lt;b&gt;Results:&lt;/b&gt; The ethyl acetate pepino fruit extract expressed the highest antioxidant activity by DPPH and CUPRAC assays. The highest TPC was obtained from the ethyl acetate extract of pepino stem (18.493 g GAE/(100 g)), while the highest TFC was obtained from the hexane extract of pepino leaves (9.541 g QE/(100 g)). &lt;b&gt;Conclusion:&lt;/b&gt; The DPPH and CUPRAC assays demonstrated that pepino exhibits potential as a source of natural antioxidants, especially in its fruit part.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological Diversity and Genetic Parameter of Okra (Abelmoschus esculentus L. Moench) Genotypes. 秋葵(Abelmoschus esculentus L. Moench)基因型的形态多样性和遗传参数。
Pakistan Journal of Biological Sciences Pub Date : 2024-01-01 DOI: 10.3923/pjbs.2024.35.45
Helvi Ardana Reswari, Muhamad Syukur, Arya Widura Ritonga, Waras Nurcholis
{"title":"Morphological Diversity and Genetic Parameter of Okra (<i>Abelmoschus esculentus</i> L. Moench) Genotypes.","authors":"Helvi Ardana Reswari, Muhamad Syukur, Arya Widura Ritonga, Waras Nurcholis","doi":"10.3923/pjbs.2024.35.45","DOIUrl":"10.3923/pjbs.2024.35.45","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Considering that the potential for okra as an anti-diabetic is very high, while okra productivity in Indonesia is still low, a plant breeding program through variety development is needed. One of the initial activities that needs to be carried out is the characterization of various genotypes, both quantitative and qualitative characters. This research aimed to obtain information on the diversity of morpho-agronomic characters in okra genotypes. &lt;b&gt;Materials and Methods:&lt;/b&gt; The experiment was conducted as a randomized block design, one factor is genotype with three replications. The materials used in this research were 20 okra genotypes. The experimental units used in this research were 60 units. Each experimental unit consists of 10 sample plants. Analysis of quantitative character variations used PKBT-STAT 3.1. Cluster analysis was carried out with PBSTAT-CL 2.1.2 with the Gower dissimilarity and average linkage clustering methods. Furthermore, analysis was carried out using SAS OnDemand for Academics to see the distinguishing characteristics between clusters. &lt;b&gt;Results:&lt;/b&gt; There were differences in okra genotypes based on qualitative and quantitative characteristics. The most diverse quantitative character is the yield component, which is the fruit character. Variance in genetic and heritability showed broad and high criteria, respectively. Based on cluster analysis results, okra genotypes were grouped into 3 clusters with a cophenetic distance value of 0.40. Cluster 1 consists of 9 genotypes. Cluster 2 consists of 10 genotypes. Cluster 3 consists of 1 genotype the Red Hill Country genotype. The grouping in cluster analysis was carried out based on leaf width, number of fruits, fruit weight, fruit diameter and carpel thickness character. &lt;b&gt;Conclusion:&lt;/b&gt; This diversity of okra germplasm can facilitate plant breeding activities in the future by selecting genotypes to serve as parents according to the objectives carried out.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oviposition Deterrent Activity of Some Wild Plants for Adult Females of Chrysomya albiceps with Medical and Veterinary Importance. 具有医疗和兽医意义的一些野生植物对白僵菌雌成虫的产卵阻遏作用
Pakistan Journal of Biological Sciences Pub Date : 2024-01-01 DOI: 10.3923/pjbs.2024.8.17
Usama Mohammed Abu El-Ghiet, Abdullah Mohammed Salman Alhuraysi, Tarek Mohamed Yousry Elsheikh, Mohamed Abdel-Monem El-Sakhawy
{"title":"Oviposition Deterrent Activity of Some Wild Plants for Adult Females of <i>Chrysomya albiceps</i> with Medical and Veterinary Importance.","authors":"Usama Mohammed Abu El-Ghiet, Abdullah Mohammed Salman Alhuraysi, Tarek Mohamed Yousry Elsheikh, Mohamed Abdel-Monem El-Sakhawy","doi":"10.3923/pjbs.2024.8.17","DOIUrl":"10.3923/pjbs.2024.8.17","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; &lt;i&gt;Chrysomya albiceps&lt;/i&gt; is widely spread worldwide, causing myiasis in both humans and animals and playing a mechanical role in the spreading of helminths, viruses and bacteria. Searching for new and safe alternative control methods is very important to eliminate the transmission of pathogens. This study aims to determine the oviposition-deterrent activity of &lt;i&gt;Juniperus procera&lt;/i&gt;, &lt;i&gt;Artemisia absinthium&lt;/i&gt;, &lt;i&gt;Rosmarinus officinalis&lt;/i&gt; and &lt;i&gt;Hypoestes forskaolii&lt;/i&gt; wild plants against adult &lt;i&gt;Chrysomya albiceps&lt;/i&gt;. &lt;b&gt;Materials and Methods:&lt;/b&gt; The effect of plant extracts from &lt;i&gt;Juniperus procera&lt;/i&gt;, &lt;i&gt;Artemisia absinthium&lt;/i&gt;, &lt;i&gt;Rosmarinus officinalis&lt;/i&gt; and &lt;i&gt;Hypoestes forskaolii&lt;/i&gt; plants were tested against adult females of &lt;i&gt;Chrysomya albiceps&lt;/i&gt; for oviposition deterrent or repellency. These extracts resulted in oviposition deterrent efficacy for adult females of &lt;i&gt;C. albiceps&lt;/i&gt; based on the plant type, plant part (leaves or stems), extract type (methanol, acetone and petroleum ether) and tested dose. &lt;b&gt;Results:&lt;/b&gt; The highest anti-oviposition activity against &lt;i&gt;C. albiceps&lt;/i&gt; females presented from &lt;i&gt;A. absinthium&lt;/i&gt; stems acetone extract at a dose of 1 mg cm&lt;sup&gt;2&lt;/sup&gt; by 100 %, while at 0.5 mg cm&lt;sup&gt;2&lt;/sup&gt; recorded remarkable repellency by 86.7% as compared with the control treatment. According to the dose-response relationship, &lt;i&gt;A. absinthium&lt;/i&gt; methanol and acetone extracts were ED&lt;sub&gt;50&lt;/sub&gt; values of 0.85, 0.319 mg cm&lt;sup&gt;2&lt;/sup&gt; (leaves) and 1.88, 0.576 mg cm&lt;sup&gt;2&lt;/sup&gt; (stems), followed by &lt;i&gt;J. procera&lt;/i&gt; methanol extract by 0.983 mg cm&lt;sup&gt;2&lt;/sup&gt; (leaves) and 0.98 mg cm&lt;sup&gt;2&lt;/sup&gt; (stems), respectively achieved highest oviposition deterrent efficiency as compared with other extracts. &lt;b&gt;Conclusion:&lt;/b&gt; The high repellency activities of these extracts can be utilized to stop &lt;i&gt;C. albiceps&lt;/i&gt; flies from laying eggs on wounds and transmitting myiasis diseases to humans and animals and could potentially replace pesticides used in the future control programs of flies.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139983491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ameliorating Effect of Apium graveolens (Celery) Extracts on IL-6 Plasma Level and Expression of Caspase 3 on Liver in Animal Model of Lead Intoxication. 芹菜提取物对铅中毒动物模型血浆中IL-6水平和肝脏中Caspase 3表达的改善作用
Pakistan Journal of Biological Sciences Pub Date : 2024-01-01 DOI: 10.3923/pjbs.2024.52.58
Rizqa Razaqtania, Danis Pertiwi, Setyo Trisnadi, Hadi Sarosa, Titiek Kusumarawati, Chodijah, Agung Putra
{"title":"Ameliorating Effect of <i>Apium graveolens</i> (Celery) Extracts on IL-6 Plasma Level and Expression of Caspase 3 on Liver in Animal Model of Lead Intoxication.","authors":"Rizqa Razaqtania, Danis Pertiwi, Setyo Trisnadi, Hadi Sarosa, Titiek Kusumarawati, Chodijah, Agung Putra","doi":"10.3923/pjbs.2024.52.58","DOIUrl":"10.3923/pjbs.2024.52.58","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Lead poisoning (Pb) is a big problem because it is found in almost all objects in daily life such as vehicle fuel, water pipes, ceramics, cosmetics and others. Continuous lead exposure can increase ROS resulting in an increase in hepatic IL-6 and caspase 3 which replaces hepatic cell apoptosis. The objective of this study was to determine the effect of &lt;i&gt;Apium graveolens&lt;/i&gt; (celery) extract on plasma IL-6 and hepatic caspase 3 levels. &lt;b&gt;Materials and Methods:&lt;/b&gt; This study used a post-test control group design. The research subjects were 20 Wistar rats that met the inclusion criteria and were divided into 4 groups randomly, namely (a) Sham group that had no treatment, (b) Negative control group was induced with lead acetate 200 mg kg&lt;sup&gt;1&lt;/sup&gt; body weight/day without any treatment (c) Positive control group and (d) Treated group. On the 15th day, blood was taken to check IL-6 levels and tissue was taken for liver caspase 3 examination by immunohistochemical method. Data analysis used the one-way ANOVA test and continued with the &lt;i&gt;post hoc&lt;/i&gt; LSD test. &lt;b&gt;Results:&lt;/b&gt; The highest mean caspase 3 expression was in the control group 45.84±4.39 pg mL&lt;sup&gt;1&lt;/sup&gt;, while the mean of IL-6 plasma level was highest in the P1 641.33±39.72 pg mL&lt;sup&gt;1&lt;/sup&gt; group. The Mann-Whitney test showed a significant difference in IL-6 levels between the study groups (p = 0.000). The Mann-Whitney test showed a significant difference in caspase 3 levels between the study groups (p = 0.000). &lt;b&gt;Conclusion:&lt;/b&gt; Giving celery extract 300 mg kg&lt;sup&gt;1&lt;/sup&gt; body weight/day affects plasma IL-6 and hepatic caspase 3 levels in lead acetate-induced rats.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140185079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilization of Sugarcane Bagasse (Saccharum officinarum Linn.) as a Carbon Source in Biofloc System of Vaname Shrimp Litopenaeus vannamei. 利用甘蔗渣(Saccharum officinarum Linn.)作为凡纳滨对虾(Litopenaeus vannamei)生物絮团系统的碳源
Pakistan Journal of Biological Sciences Pub Date : 2024-01-01 DOI: 10.3923/pjbs.2024.90.99
Yunarty, Anton, Diana Putri Renitasari, Toto Hardianto, Ardana Kurniaji
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