Pakistan Journal of Biological Sciences最新文献

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Potential Therapeutic Effect of Vitamin C on Methotrexate- Induced Damage in the Cerebral Cortex. 维生素C对甲氨蝶呤所致大脑皮质损伤的潜在治疗作用。
Pakistan Journal of Biological Sciences Pub Date : 2025-01-01 DOI: 10.3923/pjbs.2025.8.15
Ali Hassan Abdou Ali, Amany Mostafa Abo-Ouf, Heba Abdelnaser Aboelsoud, Mohammed Nawaf Alharbi, Aryaf Mohammed Almutairi, Abdullah Fahad Aljarboa, Nasser Ibrahim Alshumaymiri, Abdallah Saleh Alayyaf, Abdulaziz Muidh Alshamrani, Abdullah Taysir Alhaddad, Dhafer Mana Alamri, Abdullah Mohammed Alqahtani, Salem Abdulhadi Aldosari
{"title":"Potential Therapeutic Effect of Vitamin C on Methotrexate- Induced Damage in the Cerebral Cortex.","authors":"Ali Hassan Abdou Ali, Amany Mostafa Abo-Ouf, Heba Abdelnaser Aboelsoud, Mohammed Nawaf Alharbi, Aryaf Mohammed Almutairi, Abdullah Fahad Aljarboa, Nasser Ibrahim Alshumaymiri, Abdallah Saleh Alayyaf, Abdulaziz Muidh Alshamrani, Abdullah Taysir Alhaddad, Dhafer Mana Alamri, Abdullah Mohammed Alqahtani, Salem Abdulhadi Aldosari","doi":"10.3923/pjbs.2025.8.15","DOIUrl":"https://doi.org/10.3923/pjbs.2025.8.15","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Methotrexate is an anti-metabolic medication used to treat cancer. It causes oxidative stress in nerve tissue and has neurotoxic effects. A strong antioxidant and effective free radical scavenger is vitamin C. The current research aims to investigate the potential protective impact of vitamin C and the toxic consequences of methotrexate. &lt;strong&gt;Materials and Methods:&lt;/strong&gt; Thirty-six rats were used in this research and group one (Group 1) got no treatment at all. For 4 weeks, (Group 2) underwent a single intraperitoneal injection of methotrexate at a dose of 20 mg/kg once a week and (Group 3) got methotrexate at the same dosage as Group 2 and vitamin C (20 mg/kg) intragastrically every other day for four weeks. Rats were killed after the experiment and brain hemispheres were removed and prepared for light microscopic analysis. The cerebral hemispheres were ready for biochemical analysis to determine the brain tissue's concentrations of MDA, CAT, GSH and SOD. Data analysis was conducted using SPSS software version 20. &lt;b&gt;Results:&lt;/b&gt; In the methotrexate (2)-treated group, there were histological alterations manifested as a reduction in granular layer thickness. Purkinje cells exhibit a reduction in number, a shrinking of the cell bodies and a loss of monolaminar organization. Reduced cellularity was seen in the molecular layer. These cellular alterations are lessened and the thickness of the granular and molecular cell layers is restored following vitamin C treatment. When compared to the MTX+Vitamin C group, vitamin C greatly attenuates the biochemical and histological alterations caused by MTX. &lt;b&gt;Conclusion:&lt;/b&gt; Results concluded that although methotrexate is a toxic medication that damages the brain cortex, its toxicity is reduced when vitamin C is taken with it.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological Responses to Salinity Stress at the Germination Phase in Eight Rice (Oryza sativa L.) Cultivars Cultivated in North Sulawesi, Indonesia.
Pakistan Journal of Biological Sciences Pub Date : 2025-01-01 DOI: 10.3923/pjbs.2025.70.77
Song Ai Nio, Daniel Peter Mantilen Ludong, Ratna Siahaan
{"title":"Morphological Responses to Salinity Stress at the Germination Phase in Eight Rice (<i>Oryza sativa</i> L.) Cultivars Cultivated in North Sulawesi, Indonesia.","authors":"Song Ai Nio, Daniel Peter Mantilen Ludong, Ratna Siahaan","doi":"10.3923/pjbs.2025.70.77","DOIUrl":"https://doi.org/10.3923/pjbs.2025.70.77","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; The biodiversity of rice cultivars, including local rice from North Sulawesi, represents a potential source of germplasm for fulfilling national food needs. A few publications related to the characteristics of salinity stress resistance in rice cultivars, including local rice from North Sulawesi. This study aimed to examine the morphological response to salinity stress at the germination phase in eight rice cultivars cultivated in North Sulawesi, Indonesia. &lt;b&gt;Materials and Methods:&lt;/b&gt; The randomized block design experiment was conducted in the laboratory using eight rice cultivars (Superwin, Ombong, Temo, Burungan, Ciherang, Inpari 31, Inpago 10 and TB) using the Roll Paper Test method during the germination phase. The treatments in this experiment were salinity stress of 0, 10, 20 and 30% seawater for 14 days. The morphological characteristics evaluated as a response to salinity stress consisted of germination percentage, normal germination percentage, root length, shoot length and root-to-shoot ratio. To determine the significant differences and interactions between treatments (where, p<0.05), the data were analyzed using Analysis of Variance (ANOVA) followed by Least Significant Difference (LSD) 5%. &lt;b&gt;Results:&lt;/b&gt; Salinity stress reduced germination rates, including overall and normal germination and shorter shoot and root lengths. However, this stress caused an increase in the root-to-shoot ratio. The Roll Paper Test with salinity treatment at the germination phase could be used as a simple selection method to obtain salinity-tolerant-rice cultivars. &lt;b&gt;Conclusion:&lt;/b&gt; Shoot length and root-to-shoot ratio were potential morphological indicators for salinity tolerance in rice.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"28 2","pages":"70-77"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methamphetamine Neurotoxicity: Neurotoxic Effects, Mechanism of Toxicity, Molecular Mechanisms and Treatment Strategies. 甲基苯丙胺神经毒性:神经毒性作用、毒性机制、分子机制和治疗策略。
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.613.625
Yahya Saleh Al-Awthan, Mohamed Khalid Al-Homidi, Abdulrahman Rashid Albalawi, Khalil Ibrahim Almousi
{"title":"Methamphetamine Neurotoxicity: Neurotoxic Effects, Mechanism of Toxicity, Molecular Mechanisms and Treatment Strategies.","authors":"Yahya Saleh Al-Awthan, Mohamed Khalid Al-Homidi, Abdulrahman Rashid Albalawi, Khalil Ibrahim Almousi","doi":"10.3923/pjbs.2024.613.625","DOIUrl":"https://doi.org/10.3923/pjbs.2024.613.625","url":null,"abstract":"<p><p>Methamphetamine (METH) is a highly addictive and dangerous drug that mainly affects neurotransmitters in the brain and leads to feelings of alertness and euphoria. The METH use can lead to addiction, which has become a worldwide problem, resulting in a slew of public health and safety issues. Recent studies showed that chronic METH use can lead to neurotoxicity, neuro-inflammation and oxidative stress which can lead to neuronal injury. This review discussed the history of METH use, the link between METH use and neurotoxicity, the molecular mechanism and the different treatment strategies. This study attempted to discuss some of the drug's principal impacts and gave proof in favor of a few of the cellular and molecular causes of METH neurotoxicity. In addition, it demonstrates the most recent treatment strategies involving mitigating METH-induced neurotoxicity. However, future studies are needed to better understand the mechanism by which METH use induced neurotoxicity.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"613-625"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taxonomy, Traditional Uses and Pharmacological Properties of Ormocarpum trichocarpum (Taub.) Engl. (Fabaceae Family): A Narrative Review. 毛卡木的分类、传统用途和药理性质心血管病。(豆科):一个叙述性的回顾。
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.626.634
Alfred Maroyi
{"title":"Taxonomy, Traditional Uses and Pharmacological Properties of <i>Ormocarpum trichocarpum</i> (Taub.) Engl. (Fabaceae Family): A Narrative Review.","authors":"Alfred Maroyi","doi":"10.3923/pjbs.2024.626.634","DOIUrl":"https://doi.org/10.3923/pjbs.2024.626.634","url":null,"abstract":"<p><p>&lt;i&gt;Ormocarpum trichocarpum&lt;/i&gt; (Taub.) Engl. is a shrub or small tree harvested from the wild as a source of food, traditional medicines and wood. The present review compiles existing information on the medicinal uses and chemical and pharmacological properties of &lt;i&gt;O. trichocarpum&lt;/i&gt;. Multiple searches on existing literature on the traditional, medicinal, phytochemistry and pharmacological properties of &lt;i&gt;O. trichocarpum&lt;/i&gt; were conducted in online Databases such as Web of Science, Google Scholar, Scopus&lt;sup&gt;®&lt;/sup&gt;, SpringerLink&lt;sup&gt;®&lt;/sup&gt;, ScienceDirect&lt;sup&gt;®&lt;/sup&gt;, SciELO and PubMed&lt;sup&gt;®&lt;/sup&gt;, as well as using pre-electronic literature sources obtained from the university library. This study showed that the bark, leaves, roots, stem bark, stems and whole plant parts of &lt;i&gt;O. trichocarpum&lt;/i&gt; are used against 32 human and animal diseases and ailments.&lt;i&gt; &lt;/i&gt;The main ailments and diseases treated by &lt;i&gt;O. trichocarpum&lt;/i&gt; crude extracts include its use as an emetic, poisoning antidote, protective charm and traditional medicine for erectile dysfunction, gastrointestinal problems, prolonged labour, sexually transmitted infections and skin infections. The phytochemical evaluation of the plant revealed that it contains alkaloids, alkanes, flavonoids, phenolics, saponins, tannins, diterpenes, sesquiterpenes and bioflavonoids. Pharmacological assessments showed that the crude extracts and phytochemical compounds isolated from the species have antibacterial, antifungal, antioxidant, antiplasmodial and tyrosinase-inhibiting activities. Detailed studies focusing on toxicological evaluations, &lt;i&gt;in vivo&lt;/i&gt; and clinical research aimed at corroborating the traditional medical applications of &lt;i&gt;O. trichocarpum&lt;/i&gt; are recommended.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"626-634"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimizing Biosaka Elicitor Concentration for Enhanced Growth and Yield of Cherry Tomato (Solanum lycopersicum L. var. cerasiforme). 优化生物大阪激发子浓度促进樱桃番茄生长和产量。
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.577.586
Elimasni Elimasni, Sayyidah Afinah Salsabila Nasution
{"title":"Optimizing Biosaka Elicitor Concentration for Enhanced Growth and Yield of Cherry Tomato (<i>Solanum lycopersicum</i> L. var. <i>cerasiforme</i>).","authors":"Elimasni Elimasni, Sayyidah Afinah Salsabila Nasution","doi":"10.3923/pjbs.2024.577.586","DOIUrl":"https://doi.org/10.3923/pjbs.2024.577.586","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Prolonged utilization of chemical fertilizers can harm the soil and disturb the equilibrium of nutrients, resulting in a decline in cherry tomato yield. To enhance the growth of cherry tomato plants, it is necessary to add organic chemicals. The research aimed to determine the best elicitor biosaka concentration to apply to evoke the plant growth of cherry tomatoes (&lt;i&gt;Solanum lycopersicum&lt;/i&gt; L. var. &lt;i&gt;cerasiforme&lt;/i&gt;). &lt;b&gt;Materials and Methods:&lt;/b&gt; This study employed a complete random design with four treatments of biosaka formula: B0 (control, water), B1 (1.5 mL/L), B2 (2.5 mL/L), B3 (3.5 mL/L) and B4 (4.5 mL/L). Twenty-day-old tomato seedlings were transplanted into polybags filled with a compost and husk charcoal mixture. Biosaka application was done every four days by spraying at a 0.5 m distance, starting from the first day after transplanting. Lycopene levels were measured using a UV-vis spectrophotometer at 417 nm after a series of solvent extractions. Data were analyzed using ANOVA for significant effects. &lt;b&gt;Results:&lt;/b&gt; The biosaka concentration of 4.5 mL/L gives the highest results for the variables of treatments such as the plant height is up to 167.8 cm, the number of petioles is 62.8 petioles, the number of fruit is 19.6 and the age at flowering is 44.4 days. The biosaka concentration of 4.5 mL/L gave the highest yield for the variable plant height of 167.8 cm, the number of petioles 62.8, the number of fruits per plant 19.6, the initial flowering age of 44.4 days, the wet weight of the plant was 740 g and the dry weight of the plant was 125.1 g. &lt;b&gt;Conclusion:&lt;/b&gt; The optimal concentration of biosaka (4.5 mL/L) significantly improved cherry tomato growth and yield, resulting in increased plant height, number of petioles and fruit count. It also accelerated the initial flowering age and enhanced both wet and dry plant weights. This indicates that 4.5 mL/L of biosaka effectively promotes the growth and productivity of cherry tomato plants.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"577-586"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic Effects of Turmeric, Strawberry and Broccoli in Improving Lipid Profile in Adult Patients with Hypercholesterolemia. 姜黄、草莓和西兰花对改善成人高胆固醇血症患者血脂的协同作用。
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.587.593
Diana Krisanti Jasaputra, Theresia Monica Rahardjo, Julia Windi Gunadi, Shiela Stefani, Ivana Indriati Sutrisno
{"title":"Synergistic Effects of Turmeric, Strawberry and Broccoli in Improving Lipid Profile in Adult Patients with Hypercholesterolemia.","authors":"Diana Krisanti Jasaputra, Theresia Monica Rahardjo, Julia Windi Gunadi, Shiela Stefani, Ivana Indriati Sutrisno","doi":"10.3923/pjbs.2024.587.593","DOIUrl":"https://doi.org/10.3923/pjbs.2024.587.593","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Turmeric, strawberries and broccoli are popular in the community for their beneficial effects in improving lipid profile, but poor bioavailability and absorption of their phytochemical compounds might reduce their effects while given separately. Therefore, their combination might provide a synergistic enhancement of their property as hypolipidemic agents. This study aims to examine the effects of turmeric, strawberry and broccoli in improving lipid profile in adult patients with hypercholesterolemia. &lt;b&gt;Materials and Methods:&lt;/b&gt; Twenty hypercholesterolemic adult patients from Unggul Karsa Medika Hospital in Bandung, Indonesia were recruited for the study, they were given 600 mg/day of turmeric/curcuminoid, 100 g of fresh strawberry and 100 g of boiled broccoli for 30 days. The measurement of the patient's characteristics (body weight, height, BMI) and biochemical parameters in the blood (total cholesterol, triglyceride, LDL-C, HDL-C, SGOT, SGPT, urea and creatinine) were conducted before and after the treatment. &lt;b&gt;Results:&lt;/b&gt; The mean age of the patients was 49.15±1.9 years old, with mean height 1.56±0.014 m, weight 61.43±1.96 kg and mean body mass index 25.25±0.87 kg/m&lt;sup&gt;&lt;a href=\"86677_op\"&gt;2&lt;/a&gt;&lt;/sup&gt;. Lipid profile before the treatment: Total cholesterol 239.75±6.56 mg/dL, LDL-C 172.9±6.53 mg/dL, HDL-C 66.55±3.37 mg/dL and triglyceride 193.7±18.45 mg/dL; after the treatment: Total cholesterol 220.7±9.39 mg/dL, LDL-C 153.95±9.98 mg/dL, HDL-C 59.2±2.45 mg/dL and triglyceride 145±16.55 mg/dL. The result showed a significant reduction of total cholesterol (p = 0.014), LDL-C (p = 0.036), HDL-C (p = 0.001) and triglyceride (p = 0.015) after the treatment. Other biochemical parameters (SGOT, SGPT, urea and creatinine) showed normal levels before and after the treatment. &lt;b&gt;Conclusion:&lt;/b&gt; In summary, supplementation of turmeric, strawberry and broccoli improves lipid profile in adult patients with hypercholesterolemia.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"587-593"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histological Alterations of Cervical Cancer Following Zanthoxylum acanthopodium DC Therapy in Relation to E7, pRb, EGFR and p16 Expression. 棘花椒DC治疗后宫颈癌组织学改变与E7、pRb、EGFR和p16表达的关系
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.602.612
Rostime Hermayerni Simanullang, Jekson Martiar Siahaan, Putri Cahaya Situmorang
{"title":"Histological Alterations of Cervical Cancer Following <i>Zanthoxylum acanthopodium</i> DC Therapy in Relation to E7, pRb, EGFR and p16 Expression.","authors":"Rostime Hermayerni Simanullang, Jekson Martiar Siahaan, Putri Cahaya Situmorang","doi":"10.3923/pjbs.2024.602.612","DOIUrl":"https://doi.org/10.3923/pjbs.2024.602.612","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Cervical cancer is the second most common cancer in Indonesia, where traditional herbal treatments like &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; (andaliman) are culturally used. Investigating protein biomarkers such as E7, pRb, EGFR and p16 can help assess the efficacy of these treatments. &lt;b&gt;Materials and Methods:&lt;/b&gt; There were 5 groups in this study: 2 control groups (C- and C+) and 3 treatment groups (each receiving one of three doses). Oral administration of andaliman was performed for 30 days in cancer model rats, after which the cervix was dissected, cervical tissue was taken and immunohistochemistry repair was performed. Statistical analysis was performed using the Kruskal-Wallis test with a p<0.05. &lt;b&gt;Results:&lt;/b&gt; As &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; DC dose rose, cervical tissue E7, EGFR and p16 expression decreased. However, greater doses of this plant increased cervical tissue pRb protein. Cervical cancer histology exhibited increased nuclear size, irregular cellular structure, atypical cell shape, higher nuclear-cytoplasmic ratio and various nuclear shape variants. This herb induced tissue to show well-organized non-hyperchromatic cells that resembled normal clusters. &lt;b&gt;Conclusion:&lt;/b&gt; &lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt; DC improved cervical tissue and balanced cervical cancer biomarker proteins such E7, EGFR, pRB and p16.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"602-612"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Larval Ages and Honey Treatment on the Sex Reversal of Betta Fish (Betta splendens). 幼虫龄和蜂蜜处理对斗鱼性别逆转的影响。
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.594.601
Hartono, Adnan, Saparuddin, Yenni Yusuf
{"title":"Effect of Larval Ages and Honey Treatment on the Sex Reversal of Betta Fish (<i>Betta splendens</i>).","authors":"Hartono, Adnan, Saparuddin, Yenni Yusuf","doi":"10.3923/pjbs.2024.594.601","DOIUrl":"https://doi.org/10.3923/pjbs.2024.594.601","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Betta fish is one of the most popular ornamental fish because of their attractive body shape, especially for males. The fish development usually results in a similar number of males and females or an even higher number of females. This study aimed to determine the effect of various concentrations of honey and the age of larvae on the sex ratio of Betta fish. &lt;b&gt;Materials and Methods:&lt;/b&gt; This study was conducted using a completely randomized design with two variables; the concentrations of honey and the ages of Betta fish larvae in 3 replications. There are 45 groups in total with 20 larvae in each group. The levels of honey concentration were 0 (control), 10, 20, 30 and 40 mL/L and the levels of larval ages were 3, 4 and 5 days old. All groups were immersed for 10 hrs in the honey mix and then maintained for 4 months. Sex ratio calculation was performed after 3 months. &lt;b&gt;Results:&lt;/b&gt; The both factors have a significant effect on male sex formation. The highest number of males was produced in the honey concentration of 40 mL/L. A significant difference in the number of males was shown in the larval group of ages 3 and 4 days old but not in the groups of 5 days old. &lt;b&gt;Conclusion:&lt;/b&gt; Honey treatment and the larval age affect the formation of male Betta fish.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"594-601"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancement of Caffeine Concentration in Todolo Coffee Callus Cultures with L-Methionine and UV-Vis Spectrophotometry. 用l -蛋氨酸和紫外可见分光光度法提高咖啡愈伤组织中咖啡因的浓度。
Pakistan Journal of Biological Sciences Pub Date : 2024-11-01 DOI: 10.3923/pjbs.2024.567.576
Andi Ilham Latunra, Mustika Tuwo, Ardiansa, Dewi Sartika Amboupe
{"title":"Enhancement of Caffeine Concentration in Todolo Coffee Callus Cultures with L-Methionine and UV-Vis Spectrophotometry.","authors":"Andi Ilham Latunra, Mustika Tuwo, Ardiansa, Dewi Sartika Amboupe","doi":"10.3923/pjbs.2024.567.576","DOIUrl":"https://doi.org/10.3923/pjbs.2024.567.576","url":null,"abstract":"<p><p>&lt;b&gt;Background and Objective:&lt;/b&gt; Todolo coffee (&lt;i&gt;Coffea arabica&lt;/i&gt; L. var. typica) is the oldest commercially grown coffee in the Toraja region of South Sulawesi and is currently at risk of extinction. This study aims to induce callus from leaf explants of Todolo arabica coffee and improve the levels of caffeine compounds in Todolo coffee with the additional precursor L-methionine as elicitor. &lt;b&gt;Materials and Methods:&lt;/b&gt; This research was conducted at the Tissue Culture Laboratory, Faculty of Mathematics and Natural Sciences, Hasanuddin University. This experimental study used a Complete Randomized Design (CRD) approach. The five stages involved callus induction, elicitation with L-methionine precursor, caffeine extraction, qualitative testing using the Parry Method and quantitative analysis via UV-Vis spectrophotometry. Tools and materials were sterilized using autoclaving and media preparation followed standard procedures with varying L-methionine concentrations. Callus induction from sterilized leaf explants was done under aseptic conditions, followed by L-methionine treatment and caffeine content extraction. Caffeine was analyzed using UV-Vis spectrophotometry at 272 nm and statistical analysis was conducted using ANOVA and DMRT at a 5% significance level. &lt;b&gt;Results:&lt;/b&gt; The friable callus structure has a shiny and wavy appearance and is more easily detached, which is highly correlated with the speed of callus growth. The callus on the treatment medium is slightly yellowish-white to somewhat brownish, indicating the production of secondary metabolites. The best concentration of L-methionine treatment was 25 mg/L with a percentage of caffeine content of 0.0078%. &lt;b&gt;Conclusion:&lt;/b&gt; Results concluded that the addition of L-methionine has a significant effect on the percentage of caffeine production.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":"27 12","pages":"567-576"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association Between Opioid Receptor Kappa 1 Gene Polymorphism, Kappa Opioid Receptor Level and Dynorphin Level with Non-Suicidal Self-Injury. 阿片受体Kappa 1基因多态性、阿片受体Kappa水平和Dynorphin水平与非自杀性自伤之间的关系
Pakistan Journal of Biological Sciences Pub Date : 2024-10-01 DOI: 10.3923/pjbs.2024.518.525
Rini Gusya Liza, Yaslinda Yaunin, Yanwirasti, Rauza Sukma Rita
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