Pathogens and disease最新文献_第2页

Awakening the sleeping giant: Epstein-Barr virus reactivation by biological agents. 唤醒沉睡的巨人生物制剂导致的爱泼斯坦-巴氏病毒再活化。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae002

Omkar Indari, Subhrojyoti Ghosh, Adhiraj Singh Bal, Ajay James, Mehek Garg, Amit Mishra, Krishanpal Karmodiya, Hem Chandra Jha

{"title":"Awakening the sleeping giant: Epstein-Barr virus reactivation by biological agents.","authors":"Omkar Indari, Subhrojyoti Ghosh, Adhiraj Singh Bal, Ajay James, Mehek Garg, Amit Mishra, Krishanpal Karmodiya, Hem Chandra Jha","doi":"10.1093/femspd/ftae002","DOIUrl":"10.1093/femspd/ftae002","url":null,"abstract":"Epstein-Barr virus (EBV) may cause harm in immunocompromised conditions or on stress stimuli. Various chemical agents have been utilized to induce the lytic cycle in EBV-infected cells. However, apart from chemical agents and external stress stimuli, certain infectious agents may reactivate the EBV. In addition, the acute infection of other pathogens may provide suitable conditions for EBV to thrive more and planting the roots for EBV-associated pathologies. Various bacteria such as periodontal pathogens like Aggregatibacter, Helicobacter pylori, etc. have shown to induce EBV reactivation either by triggering host cells directly or indirectly. Viruses such as Human simplex virus-1 (HSV) induce EBV reactivation by HSV US3 kinase while other viruses such as HIV, hepatitis virus, and even novel SARS-CoV-2 have also been reported to cause EBV reactivation. The eukaryotic pathogens such as Plasmodium falciparum and Aspergillus flavus can also reactivate EBV either by surface protein interaction or as an impact of aflatoxin, respectively. To highlight the underexplored niche of EBV reactivation by biological agents, we have comprehensively presented the related information in this review. This may help to shedding the light on the research gaps as well as to unveil yet unexplored mechanisms of EBV reactivation.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural products and derivatives as Japanese encephalitis virus antivirals. 作为日本脑炎病毒抗病毒药物的天然产品及其衍生物。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae022

Yunqi Mi, Yan Guo, Xuliang Luo, Yang Bai, Haonan Chen, Meihua Wang, Yang Wang, Jiao Guo

引用次数: 0

Optimal protein allocation controls the inhibition of GltA and AcnB in Neisseria gonorrhoeae. 最佳蛋白质分配控制着淋病奈瑟菌中 GltA 和 AcnB 的抑制作用。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae023

Nabia Shahreen, Niaz Bahar Chowdhury, Rajib Saha

{"title":"Optimal protein allocation controls the inhibition of GltA and AcnB in Neisseria gonorrhoeae.","authors":"Nabia Shahreen, Niaz Bahar Chowdhury, Rajib Saha","doi":"10.1093/femspd/ftae023","DOIUrl":"10.1093/femspd/ftae023","url":null,"abstract":"Neisseria gonorrhea (Ngo) is a major concern for global public health due to its severe implications for reproductive health. Understanding its metabolic phenotype is crucial for comprehending its pathogenicity. Despite Ngo's ability to encode tricarboxylic acid (TCA) cycle proteins, GltA and AcnB, their activities are notably restricted. To investigate this phenomenon, we used the iNgo_557 metabolic model and incorporated a constraint on total cellular protein content. Our results indicate that low cellular protein content severely limits GltA and AcnB activity, leading to a shift toward acetate overflow for Adenosine triphosphate (ATP) production, which is more efficient in terms of protein usage. Surprisingly, increasing cellular protein content alleviates this restriction on GltA and AcnB and delays the onset of acetate overflow, highlighting protein allocation as a critical determinant in understanding Ngo's metabolic phenotype. These findings underscore the significance of Ngo's metabolic adaptation in light of optimal protein allocation, providing a blueprint to understand Ngo's metabolic landscape.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142351525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multispecies bacterial invasion of human host cells. 多菌种细菌入侵人类宿主细胞。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae012

Charlotte Abell-King, Alaska Pokhrel, Scott A Rice, Iain G Duggin, Bill Söderström

{"title":"Multispecies bacterial invasion of human host cells.","authors":"Charlotte Abell-King, Alaska Pokhrel, Scott A Rice, Iain G Duggin, Bill Söderström","doi":"10.1093/femspd/ftae012","DOIUrl":"10.1093/femspd/ftae012","url":null,"abstract":"Urinary tract infection (UTI), one of the most common bacterial infections worldwide, is a typical example of an infection that is often polymicrobial in nature. While the overall infection course is known on a macroscale, bacterial behavior is not fully understood at the cellular level and bacterial pathophysiology during multispecies infection is not well characterized. Here, using clinically relevant bacteria, human epithelial bladder cells and human urine, we establish co-infection models combined with high resolution imaging to compare single- and multi-species bladder cell invasion events in three common uropathogens: uropathogenic Escherichia coli (UPEC), Klebsiella pneumoniae and Enterococcus faecalis. While all three species invaded the bladder cells, under flow conditions the Gram-positive E. faecalis was significantly less invasive compared to the Gram-negative UPEC and K. pneumoniae. When introduced simultaneously during an infection experiment, all three bacterial species sometimes invaded the same bladder cell, at differing frequencies suggesting complex interactions between bacterial species and bladder cells. Inside host cells, we observed encasement of E. faecalis colonies specifically by UPEC. During subsequent dispersal from the host cells, only the Gram-negative bacteria underwent infection-related filamentation (IRF). Taken together, our data suggest that bacterial multispecies invasions of single bladder cells are frequent and support earlier studies showing intraspecies cooperation on a biochemical level during UTI.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11180983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141093318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sphingosine kills intracellular Pseudomonas aeruginosa and Staphylococcus aureus. 鞘氨醇能杀死细胞内的绿脓杆菌和金黄色葡萄球菌。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae016

Helene May, Yongjie Liu, Stephanie Kadow, Michael J Edwards, Simone Keitsch, Barbara Wilker, Markus Kamler, Heike Grassmé, Yuqing Wu, Erich Gulbins

引用次数: 0

Uropathogenic Escherichia coli causes significant urothelial damage in an ex vivo porcine bladder model, with no protective effect observed from cranberry or d-mannose. 在体外猪膀胱模型中，致病性大肠杆菌会造成严重的尿道损伤，而蔓越莓或 D-甘露糖均无保护作用。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae026

Jenane Konesan, Kate H Moore, Kylie J Mansfield, Lu Liu

{"title":"Uropathogenic Escherichia coli causes significant urothelial damage in an ex vivo porcine bladder model, with no protective effect observed from cranberry or d-mannose.","authors":"Jenane Konesan, Kate H Moore, Kylie J Mansfield, Lu Liu","doi":"10.1093/femspd/ftae026","DOIUrl":"10.1093/femspd/ftae026","url":null,"abstract":"Urinary tract infections (UTIs), primarily caused by uropathogenic Escherichia coli (UPEC), have an unclear impact on bladder mucosal physiology. This study investigates UPEC's effects on the urothelium and lamina propria using an ex vivo porcine bladder model. Bladder mucosal strips were analysed for contractile responses to acetylcholine, serotonin, and neurokinin A. Given rising antibiotic resistance, non-antibiotic agents such as cranberry and d-mannose were also evaluated for their potential to prevent UPEC-induced damage. The findings of the current study revealed that UPEC significantly compromised urothelial integrity, barrier function, and permeability, with loss of urothelial cells, uroplakins, and tight junction protein ZO-1 expression. Additionally, infected bladders exhibited a markedly enhanced contractile response to serotonin compared to uninfected controls. Notably, neither cranberry nor d-mannose offered protection against UPEC-mediated damage or mitigated the heightened serotonin-induced contractility. This study provides novel insights into how UPEC disrupts bladder cell biology and highlights the possible involvement of serotonin in the pathophysiology of UTIs.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on Zika vaccine development. 寨卡疫苗研发综述。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftad036

Zhe-Yu Peng, Song Yang, Hong-Zheng Lu, Lin-Min Wang, Ni Li, Hai-Ting Zhang, Si-Yu Xing, Yi-Nan Du, Sheng-Qun Deng

引用次数: 0

Advances in vaccine development for Chlamydia trachomatis. 沙眼衣原体疫苗开发进展。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae017

Taylor B Poston

{"title":"Advances in vaccine development for Chlamydia trachomatis.","authors":"Taylor B Poston","doi":"10.1093/femspd/ftae017","DOIUrl":"10.1093/femspd/ftae017","url":null,"abstract":"Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection globally. Antibiotic treatment is highly effective, but infection is often asymptomatic resulting in most individuals going undetected and untreated. This untreated infection can ascend to the upper female genital tract to cause pelvic inflammatory disease, tubal factor infertility, and ectopic pregnancy. Chlamydia screening and treatment programs have failed to control this epidemic and demonstrate the need for an efficacious vaccine to prevent transmission and disease. Animal models and human epidemiological data reveal that natural immunity can provide partial or short-lived sterilizing immunity. These data further demonstrate the importance of eliciting interferon gamma (IFNγ)-producing cluster of differentiation 4 (CD4) T cells (Th1 and Th1/17 cells) that can likely synergize with antibody-mediated opsonophagocytosis to provide optimal protection. These studies have guided preclinical rational vaccine design for decades and the first Phase 1 clinical trials have recently been completed. Recent advances have led to improvements in vaccine platforms and clinically safe adjuvants that help provide a path forward. This review describes vaccine models, correlates of immunity, antigen and adjuvant selection, and future clinical testing for Chlamydia vaccine development.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11338180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico design and analysis of a multiepitope vaccine against Chlamydia. 针对衣原体的多表位疫苗的硅学设计和分析。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae015

Tayhlor Tanner, F N U Medhavi, Shakyra Richardson, Yusuf O Omosun, Francis O Eko

{"title":"In silico design and analysis of a multiepitope vaccine against Chlamydia.","authors":"Tayhlor Tanner, F N U Medhavi, Shakyra Richardson, Yusuf O Omosun, Francis O Eko","doi":"10.1093/femspd/ftae015","DOIUrl":"10.1093/femspd/ftae015","url":null,"abstract":"Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterial infection worldwide, potentially leading to severe pathologies including pelvic inflammatory disease, ectopic pregnancy, and tubal infertility if left untreated. Current strategies, including screening and antibiotics, have limited effectiveness due to high rates of asymptomatic cases and logistical challenges. A multiepitope prophylactic vaccine could afford long-term protection against infection. Immunoinformatic analyses were employed to design a multiepitope Chlamydia vaccine antigen. B- and T-cell epitopes from five highly conserved and immunogenic Ct antigens were predicted and selected for the vaccine design. The final construct, adjuvanted with cholera toxin A1 subunit (CTA1), was further screened for immunogenicity. CTA1-MECA (multiepitope Chlamydia trachomatis antigen) was identified as antigenic and nonallergenic. A tertiary structure was predicted, refined, and validated as a good quality model. Molecular docking exhibited strong interactions between the vaccine and toll-like receptor 4 (TLR4). Additionally, immune responses consistent with protection including IFN-γ, IgG + IgM antibodies, and T- and B-cell responses were predicted following vaccination in an immune simulation. Expression of the construct in an Escherichia coli expression vector proved efficient. To further validate the vaccine efficacy, we assessed its immunogenicity in mice. Immunization with CTA1-MECA elicited high levels of Chlamydia-specific antibodies in mucosal and systemic compartments.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disrupting quorum sensing as a strategy to inhibit bacterial virulence in human, animal, and plant pathogens. 将破坏法定人数感应作为抑制人类、动物和植物病原体中细菌毒力的一种策略。

IF 2.7 4区医学

Pathogens and disease Pub Date : 2024-02-07 DOI: 10.1093/femspd/ftae009

Mélanie Gonzales, Baptiste Kergaravat, Pauline Jacquet, Raphaël Billot, Damien Grizard, Éric Chabrière, Laure Plener, David Daudé

{"title":"Disrupting quorum sensing as a strategy to inhibit bacterial virulence in human, animal, and plant pathogens.","authors":"Mélanie Gonzales, Baptiste Kergaravat, Pauline Jacquet, Raphaël Billot, Damien Grizard, Éric Chabrière, Laure Plener, David Daudé","doi":"10.1093/femspd/ftae009","DOIUrl":"10.1093/femspd/ftae009","url":null,"abstract":"The development of sustainable alternatives to conventional antimicrobials is needed to address bacterial virulence while avoiding selecting resistant strains in a variety of fields, including human, animal, and plant health. Quorum sensing (QS), a bacterial communication system involved in noxious bacterial phenotypes such as virulence, motility, and biofilm formation, is of utmost interest. In this study, we harnessed the potential of the lactonase SsoPox to disrupt QS of human, fish, and plant pathogens. Lactonase treatment significantly alters phenotypes including biofilm formation, motility, and infection capacity. In plant pathogens, SsoPox decreased the production of plant cell wall degrading enzymes in Pectobacterium carotovorum and reduced the maceration of onions infected by Burkholderia glumae. In human pathogens, lactonase treatment significantly reduced biofilm formation in Acinetobacter baumannii, Burkholderia cepacia, and Pseudomonas aeruginosa, with the cytotoxicity of the latter being reduced by SsoPox treatment. In fish pathogens, lactonase treatment inhibited biofilm formation and bioluminescence in Vibrio harveyi and affected QS regulation in Aeromonas salmonicida. QS inhibition can thus be used to largely impact the virulence of bacterial pathogens and would constitute a global and sustainable approach for public, crop, and livestock health in line with the expectations of the One Health initiative.","PeriodicalId":19795,"journal":{"name":"Pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140898372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0