Pancreas最新文献

{"title":"Serum Calcium and Hospital Stay in Acute Pancreatitis: A Nonlinear Association With an Inflection Point.","authors":"Mengdi Liu, Lingyan Qiu","doi":"10.1097/MPA.0000000000002576","DOIUrl":"10.1097/MPA.0000000000002576","url":null,"abstract":"<p><strong>Background: </strong>Acute pancreatitis (AP) is a critical inflammatory condition associated with significant morbidity and mortality. Calcium ions (Ca2+) have been implicated in the pathophysiology of AP, with potential predictive value for disease severity and patient outcomes, including hospital length of stay (LOS).</p><p><strong>Objective: </strong>The study aimed to investigate the relationship between serum calcium levels and LOS in patients with AP, considering the nonlinear trends and potential threshold effects of Ca2+.</p><p><strong>Methods: </strong>We conducted a retrospective cohort study including 717 patients with AP admitted to the gastroenterology departments of Ningbo Second Hospital from July 2020 to June 2023. Data on demographic characteristics, medical history, and laboratory parameters were collected. The relationship between Ca2+ levels and LOS was analyzed using univariate and multivariate regression models, with adjustments for potential confounders. Nonlinear trends were explored using a Generalized Additive Model (GAM) and piecewise linear regression.</p><p><strong>Results: </strong>The study revealed a significant nonlinear relationship between Ca2+ levels and LOS, with distinct inflection points at Ca2+ concentrations of 1.7 and 2.5 mmol/L. Below 1.7 mmol/L, Ca2+ showed no significant association with LOS, whereas between 1.7 and 2.5 mmol/L, a significant negative effect was observed. Above 2.5 mmol/L, a significant positive effect emerged. The study also identified significant effect size variations across different subgroups, including sex, age, BMI, and comorbidities.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e440-e446"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145714922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence, Predictors, Management, and Outcome of Paralytic Ileus in Acute Pancreatitis.","authors":"Suprabhat Giri, Chandramauli Mishra, Sukhraj Pal Singh, Saroj Kanta Sahu, Dibya Lochan Praharaj, Bipadabhanjan Mallick, Preetam Nath, Swati Das, Vedavyas Mohapatra, Sarat Chandra Panigrahi, Anil Chandra Anand, Manoj Kumar Sahu, Enrique de-Madaria","doi":"10.1097/MPA.0000000000002594","DOIUrl":"10.1097/MPA.0000000000002594","url":null,"abstract":"<p><strong>Background: </strong>Paralytic ileus is a frequent complication of acute pancreatitis (AP), contributing to increased morbidity, nutritional compromise, and risk of infection. Despite its clinical relevance, data on its incidence, predictors, management, and outcomes in AP remain limited.</p><p><strong>Methods: </strong>We conducted a retrospective study of 426 adult patients admitted with AP over a 3-year period at a tertiary-care center. The primary outcome was paralytic ileus; secondary outcomes included infected necrosis, intervention, and mortality. Multivariate logistic regression identified independent predictors expressed as adjusted odds ratio (aOR).</p><p><strong>Results: </strong>Paralytic ileus occurred in 167/426 (39.2%) patients. Patients with ileus were more frequently male, obese, and had higher rates of SIRS compared with those without ileus. They also exhibited significantly higher leukocyte counts, neutrophil-lymphocyte ratio (NLR), serum creatinine, and lower serum albumin and sodium levels. In multivariate analysis, obesity (aOR: 2.329), NLR (aOR: 1.131), serum albumin (aOR: 0.342), and serum sodium (aOR: 0.802) were identified as independent predictors. An NLR of 9.5 had a sensitivity of 82.6% and a specificity of 76.1% for predicting paralytic ileus. Conservative management resolved ileus in 85% of cases, 6% responded to neostigmine, while 9% with unresolved ileus succumbed to multiorgan failure. Paralytic ileus was independently associated with increased risk of infected necrosis (aOR: 3.62), intervention (aOR: 4.43), and mortality (aOR: 4.71).</p><p><strong>Conclusion: </strong>Paralytic ileus affects a significant proportion of patients with moderate to severe AP and is a marker of local and systemic complications. Paralytic ileus was an independent predictor of infected necrosis, intervention, and mortality, underscoring its prognostic relevance.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e347-e351"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145445027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementation of an Electronic Clinical Decision Support Order Set for Acute Pancreatitis in an Integrated Health Care System: A Mixed Methods Analysis.","authors":"Sarvanand Patel, Bechien U Wu, Tiffany Luong, Qiaoling Chen, Wansu Chen, Eva Lustigova","doi":"10.1097/MPA.0000000000002560","DOIUrl":"10.1097/MPA.0000000000002560","url":null,"abstract":"<p><strong>Background: </strong>Standardized admission order entry systems (OES) and clinical decision support (CDS) tools can facilitate implementation of evidence-based practice. We conducted a mixed-methods analysis incorporating quantitative analysis of OES/CDS usage as well as qualitative analysis to identify barriers and facilitators related to usage of acute pancreatitis (AP)-specific OES-CDS.</p><p><strong>Materials and methods: </strong>Quantitative analysis: A retrospective cohort study of hospitalized encounters for AP (defined by diagnosis codes and lipase or amylase >3x normal) was conducted across 12 hospitals within an integrated health system from September 2014 through October 2022 to evaluate adoption of the AP OES/CDS, relationship to length-of-stay (LOS≥7 days), and in-hospital mortality adjusted for patient clinical and demographic characteristics. Qualitative analysis: Semistructured interviews of focus groups identified themes related to perceived benefits, barriers, and improvements to the OES/CDS.</p><p><strong>Results: </strong>Among 16,874 AP hospitalizations during the study, annual use of the AP OES/CDS peaked in 2021 at 7.4%. Use of AP OES/CDS was associated with reduction in prolonged LOS (OR: 0.81, 95% CI: 0.69-0.94). This finding was consistent after adjusting age, sex, race/ethnicity, and comorbidity indices. No significant difference in mortality was associated with OES usage (OR: 0.89, 95% CI: 0.61-1.32). Barriers to OES adoption included lack of awareness, competition with other tools, and complexity of patient case-mix.</p><p><strong>Conclusion: </strong>Although adoption was limited, usage of an AP OES/CDS tool was associated with a decrease in prolonged length-of-stay. Future efforts to expand the reach of such instruments through provider awareness and refinement of the instrument may facilitate broader impact for management of patients with acute pancreatitis.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e417-e422"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145844115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoscopic Management of Chronic Pancreatitis in Children: A Single-center Experience Over a Decade.","authors":"Malla Va Gangadhar Rao, Ujjal Poddar, S Rakesh Kumar, Arghya Samanta, Pritam Das, Anshu Srivastava, Moinak Sen Sarma, Prabhakar Mishra, Vivek Saraswat, Surender K Yachha, Samir Mohindra","doi":"10.1097/MPA.0000000000002585","DOIUrl":"10.1097/MPA.0000000000002585","url":null,"abstract":"<p><strong>Background: </strong>Chronic pancreatitis (CP) complications in adults are successfully managed with endoscopic retrograde cholangiopancreatography (ERCP). Data are limited on the success of ERCP in children with CP. We reviewed our experience to ascertain the success, safety, and effectiveness of ERCP for relieving pain and its effect on exocrine and endocrine insufficiency.</p><p><strong>Methods: </strong>Retrospective analysis of a prospectively kept database of children (≤18 y) with CP who underwent therapeutic ERCP between 2010 and 2020 was reviewed. Clinical details, laboratory and radiologic investigations, procedural details, and follow-up data were retrieved from manual and electronic records. Categorical and numerical data were expressed as proportion and mean ± SD.</p><p><strong>Results: </strong>Sixty-five children (60% males), age 12.8 ± 2.9years, were included. The most common etiology was idiopathic (n = 45, 69%). Technical, endoscopic, and clinical success rates were 95.3%, 92.3%, and 85.6%, respectively. Only 4 patients (6.1%) had major complications. Over a median follow-up of 24 months, therapeutic ERCP resulted in significant pain relief, with nonprogression of exocrine and endocrine insufficiency. Recurrence of symptoms was noted in 10%, which were managed successfully with repeat ERCP.</p><p><strong>Conclusion: </strong>Therapeutic ERCP is a safe and effective therapy for the management of CP in children, with significant improvement in pain, and could influence stabilization of exocrine and endocrine functions.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e362-e367"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Transcription Factor TFAP2A Induces M2 Macrophage Polarization by Upregulating EPS8L3 Expression, Thereby Accelerating the Malignant Progression of Pancreatic Ductal Adenocarcinoma.","authors":"Yilihamu Maimaiti, Zaiyinuer Abuduwaili, Dong Yan, Xiaogang Dong, Qing Tong, Huan Yang, Panpan Kong, Yimamumaimaitijiang Abula","doi":"10.1097/MPA.0000000000002577","DOIUrl":"10.1097/MPA.0000000000002577","url":null,"abstract":"<p><strong>Background: </strong>Pancreatic ductal adenocarcinoma (PDAC), the main pancreatic cancer type, was highly aggressive and lethal. Studies showed that the epidermal growth factor receptor pathway substrate 8-Like protein 3 (EPS8L3) was significantly upregulated in PDAC. This study aimed to explore how EPS8L3 promoted PDAC progression.</p><p><strong>Methods: </strong>First, bioinformatics analysis, qRT-PCR, and western blot (WB) techniques were utilized to ascertain the expression profile of EPS8L3 in clinical samples and cells of PDAC. Subsequently, EdU proliferation assays, cell apoptosis detection, glycolysis assay kits, in vivo xenograft tumor experiments, and immunohistochemical (IHC) staining were conducted to explore the impact of EPS8L3 silencing on PDAC cell proliferation, apoptosis, glycolytic pathway, and tumor growth in vivo. Meanwhile, flow cytometry was employed to analyze the expression of CD163, a marker of macrophage M2 polarization. Furthermore, with the aid of JASPAR and GEPIA websites, combined with chromatin immunoprecipitation (Ch-IP) experiments and dual luciferase reporter gene experiments, the interaction between transcription factor AP-2α (TFAP2A) and EPS8L3 was further confirmed. Finally, a rescue experiment was performed with EPS8L3 overexpression in TFAP2A-knockdown cells to validate the potential impact of EPS8L3 on TFAP2A function.</p><p><strong>Results: </strong>EPS8L3 was highly expressed in PDAC tumors and PDAC cells, and its silencing effectively inhibited the proliferation of PDAC cells and promoted their apoptosis. Furthermore, the glycolytic pathway in PDAC cells, tumor growth in vivo, and M2 polarization of macrophages were also blocked by EPS8L3 knockdown. TFAP2A interacted with EPS8L3 and positively regulated its expression. Overexpression of EPS8L3 restored the effects of TFAP2A knockdown on PDAC cell progression.</p><p><strong>Conclusions: </strong>TFAP2A positively regulated EPS8L3 to facilitate M2 polarization of macrophages and the malignant progression of PDAC cells.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":"55 4","pages":"e385-e396"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-stage Pancreatic Ductal Adenocarcinoma Diagnosis Is Inversely Associated With Travel Distance and Travel Time.","authors":"Bradford Chong, Rachel Baccile, Anjani Kapadia, Mandela Gadri, Rahul Sandella, Aaron Goffinet, Sebastian Dobrow, Jeremy Klein, Marynia Kolak, Sonia S Kupfer","doi":"10.1097/MPA.0000000000002601","DOIUrl":"10.1097/MPA.0000000000002601","url":null,"abstract":"<p><strong>Objective: </strong>Leveraging geospatial data and SDOH (social determinants of health) indices may enhance understanding of the interactions between geographic and social factors that facilitate early cancer diagnosis. We sought to characterize associations between travel distance, time, and early-stage pancreatic ductal adenocarcinoma (PDAC) diagnosis.</p><p><strong>Methods: </strong>This was a retrospective study of PDAC patients at an urban academic medical center between January 1, 2012, and February 31, 2022. We geocoded patients to census tracts to calculate car travel distance and time. For residents within city limits, we calculated public transit time. The primary outcome was stage I diagnosis. Logistic regression was used for travel measures, and multivariable analyses adjusted for age, diagnosis year, and insurance. Sensitivity analyses were done using the Area Deprivation Index and the SDOH Atlas.</p><p><strong>Results: </strong>We identified 694 PDAC patients, with 146 (23.2%) having a stage I diagnosis. Residing >25 miles and car travel time >45 minutes were associated with early diagnosis (OR: 2.35, 95% CI [1.41, 3.98], OR 2.49, 95% CI [1.53, 4.14] respectively). On sensitivity analyses, these associations persisted in low to moderate deprivation neighborhoods. Among city residents, there was no association between travel measures and early diagnosis.</p><p><strong>Conclusions: </strong>Patients in less socioeconomically deprived neighborhoods are diagnosed at an earlier stage despite traveling longer distances and times.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e359-e361"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide Association Analysis for Organ Failure in Acute Pancreatitis.","authors":"Apostolos Gaitanidis, Mathias A Christensen, Katherine Albutt, Casey M Luckhurst, Carlos Fernandez-Del Castillo, Yasmin Hernandez-Barco, George C Velmahos, Peter J Fagenholz","doi":"10.1097/MPA.0000000000002581","DOIUrl":"10.1097/MPA.0000000000002581","url":null,"abstract":"<p><strong>Background: </strong>Patients with acute pancreatitis (AP) develop widely variable severity of organ failure. We hypothesized that genetic factors may influence the development of organ failure in AP. In this study, we identify candidate loci associated with organ failure in AP using genome-wide association analyses (GWAS) and transcriptomic analyses.</p><p><strong>Methods: </strong>Subjects enrolled in the Mass General Brigham Biobank were retrospectively queried to determine those with a history of AP. Patients with AP were categorized according to the presence of respiratory or renal failure during the first week of AP. Patients were further divided into 2 groups based on the etiology of AP (ie, biliary AP and AP of all other etiologies) and analyzed separately. Meta-analysis of the 2 groups identified candidate loci with P <1e-05. Genes with eQTLs (expression quantitative trait loci) in candidate loci in immune cells were selected. The differential expression of these genes in AP was assessed using transcriptomic data from the Gene Expression Omnibus (GEO) database.</p><p><strong>Results: </strong>Overall, 665 patients were identified, of which 211 had biliary AP (199 mild, 12 severe) and 454 had AP of other etiologies (428 mild, 26 severe). Nine candidate loci were identified, with rs12185172 on chromosome 16 having the highest association among all SNPs (beta=1.46, P =1.13e-06). Five genes had eQTLs in the candidate loci. CHD9 (adj. P =5.64e-04) , FYB (adj. P =5.09e-03), and MMADHC (adj. P =2.44e-03) were differentially expressed in AP.</p><p><strong>Conclusions: </strong>We performed a GWAS examining organ failure in AP and identified 9 candidate loci, which did not pass the genome-wide significance threshold. Three candidate genes, CHD9 , FYB , and MMADHC , were identified through eQTL and differential gene expression analyses. These may represent novel therapeutic targets for organ failure in AP.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e411-e416"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145286704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Coexistence of Diabetes Mellitus and Low Fecal Elastase Is Associated With Increased Pancreatic Cancer Risk: A Retrospective, Real-world Cohort Study.","authors":"Chukwuemeka Ogbu, Yichen Wang, Oyedotun Babajide, Chukwunonso Ezeani, Lekhya Kollu, Osayande Osagiede, Philip N Okafor","doi":"10.1097/MPA.0000000000002592","DOIUrl":"10.1097/MPA.0000000000002592","url":null,"abstract":"<p><strong>Objectives: </strong>Diabetes mellitus and chronic pancreatitis are established risk factors for pancreatic ductal adenocarcinoma (PDAC). The co-occurrence of exocrine and endocrine pancreatic dysfunction may increase the risk of progression to PDAC and could play a role in early detection. We sought to explore this hypothesis by comparing PDAC risk among patients with diabetes mellitus (DM) and low fecal elastase (FE) in a real-world cohort of patients.</p><p><strong>Materials and methods: </strong>A retrospective cohort study was conducted using the TriNetX research network including adults with continuous follow-up from 2016 to 2023. The cohort was stratified into 4 groups based on the presence or absence of DM, and normal FE (>200 µg/g) or low FE (<200 µg/g). The 4 groups included: (1) low FE and DM, (2) low FE without DM, (3) normal FE and DM, (4) normal FE without DM. Propensity score matching was performed to balance covariates including age, sex, race, comorbidities to allow for a 6-way comparison of PDAC risk between groups. Time-to-event analyses were conducted with Kaplan-Meier curves and Cox proportional hazards models to estimate hazard ratios (HR) and 95% CIs for the primary outcome of PDAC incidence.</p><p><strong>Results: </strong>Of the study cohort, 29,207 had fecal elastase levels. Low FE (<200 µg/g) was observed in 33% (n = 9585) of these patients. After 6-way comparisons, we observed a higher PDAC risk among individuals with both DM and low FE compared with other groups. The highest PDAC risk was observed in patients with both a low FE (<200 µg/g) and DM compared with those with a normal FE and without a history of DM (HR: 3.68; 95% CI, 2.07-6.53; P < 0.001). Sensitivity analysis using a stricter fecal elastase cutoff of <100 µg/g showed a similar finding, with the highest hazard ratio for PDAC among patients with both DM and very low FE levels (HR: 3.07, 95% CI, 1.37-6.91; P = 0.004).</p><p><strong>Conclusions: </strong>The coexistence of low fecal elastase and DM amplifies the risk of pancreatic ductal adenocarcinoma in our cohort of patients. These results suggest that fecal elastase values may be used to enrich the diabetic population for future PDAC risk stratification, though more studies are needed to validate these findings.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e352-e358"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145489962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association Between Serum Uric Acid to Creatinine Ratio and the Severity of Acute Pancreatitis.","authors":"Yang Xu, Yingluo Wang, Qian Yu, Xin Huang, Jingyuan Li, Xiaoqing Zhang, Xirong Chen, Ying Chen","doi":"10.1097/MPA.0000000000002567","DOIUrl":"10.1097/MPA.0000000000002567","url":null,"abstract":"<p><strong>Objective: </strong>To examine the relationship between the serum uric acid to creatinine ratio (SUA/SCr) and the severity of acute pancreatitis.</p><p><strong>Methods: </strong>A total of 1612 patients diagnosed with acute pancreatitis at the Affiliated Hospital of Qingdao University were enrolled in this study. Patients were categorized into 2 groups: mild acute pancreatitis (MAP) and severe acute pancreatitis (SAP). Demographic data, including sex and age, along with biochemical indicators such as white blood cell count, arterial blood pH, glucose, triglycerides, and serum calcium, were collected and analyzed.</p><p><strong>Results: </strong>Among the 1612 patients, those with lower SUA/SCr levels were at an increased risk of developing severe acute pancreatitis ( P =0.026, OR=0.81, 95% CI: 0.72-0.98). Restricted cubic spline (RCS) analysis revealed a nonlinear, U-shaped relationship between SUA/SCr and the risk of severe acute pancreatitis. A clinical prediction model was developed, achieving an area under the curve (AUC) of 0.81.</p><p><strong>Conclusion: </strong>Lower SUA/SCr levels are associated with an elevated risk of severe acute pancreatitis. These findings suggest that SUA/SCr may serve as a valuable biomarker for predicting the severity of acute pancreatitis in clinical practice.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":" ","pages":"e447-e452"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12975011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of Lymphoid Enhancer-binding Factor 1 Inhibits Pancreatic Adenocarcinoma Growth and Neoangiogenesis by Curbing Notch1 and Nuclear Factor Kappa B Signaling Pathways.","authors":"Guanglun Ping, Guoyong Cai, Ying Wei, Xiangli Mo, Li Liu, Wencui Zhu, Yi Zhang","doi":"10.1097/MPA.0000000000002562","DOIUrl":"10.1097/MPA.0000000000002562","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the impaction of LEF1 for malignant development of pancreatic adenocarcinoma (PAAD) and its specific mechanisms.</p><p><strong>Materials and methods: </strong>The expression of LEF1 in PAAD and normal tissues was inspected by bioinformatics, qRT-PCR, and western blot (WB). AsPC-1 and BxPC-3 were selected for further knockdown study. The post-knockdown cellular malignant progression was evaluated by CCK-8, EdU, clonogenic assay, wound healing, Transwell, Calcein AM/PI staining, and LDH release assays. Conditioned medium (CM) of PAAD after LEF1 knockdown was collected to culture HUVEC for evaluating the effect on angiogenesis. Immunofluorescence assay and WB were employed to detect the changes of Notch1 and NF-κB pathway. Finally, a nude mouse tumor xenograft model was established to verify the in vivo suppressive effect of knocking down LEF1 on PAAD.</p><p><strong>Results: </strong>LEF1 is highly expressed in PAAD. The proliferation, migration, and invasion of AsPC-1/sh-LEF1 and BxPC-3/sh-LEF1 were inhibited, and the apoptosis was significantly increased. The CM of AsPC-1/sh-LEF1 and BxPC-3/sh-LEF1 significantly inhibited HUVEC migration and angiogenesis. The intranuclear expression of Notch1, NICD, Hes1, and P65 proteins were reduced, indicating that LEF1 downregulation inhibited the activation of the Notch1 and NF-κB. Finally, the inhibitory effect of LEF1 downregulation on PAAD growth was further verified in vivo, confirming the important role of LEF1 in PAAD development.</p><p><strong>Conclusions: </strong>Knockdown of LEF1 can inhibit the growth and neoangiogenesis of PAAD by inhibiting Notch1 and NF-κB, thus inhibiting the malignant progression of PAAD.</p>","PeriodicalId":19733,"journal":{"name":"Pancreas","volume":"55 4","pages":"e397-e410"},"PeriodicalIF":2.2,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12975020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}