Open Medicine最新文献

{"title":"The effects of enhanced external counter-pulsation on post-acute sequelae of COVID-19: A narrative review.","authors":"Jiecheng Huang, Yuxuan Fan, Yongshun Wang, Jingjin Liu","doi":"10.1515/med-2024-1067","DOIUrl":"10.1515/med-2024-1067","url":null,"abstract":"<p><p>Some of the millions of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have developed new sequelae after recovering from the initial disease, termed post-acute sequelae of coronavirus disease 2019 (PASC). One symptom is anxiety, which is likely due to three etiologies: brain structural changes, neuroendocrine disruption, and neurotransmitter alterations. This review provides an overview of the current literature on the pathophysiological pathways linking coronavirus disease 2019 to anxiety, as well as the possible mechanisms of action in which an increasingly scrutinized treatment method, enhanced external counter-pulsation (EECP), is able to alleviate anxiety. SARS-CoV-2 triggers increased inflammatory cytokine production, as well as oxidative stress; these processes contribute to the aforementioned three etiologies. The potential treatment approach of EECP, involving sequenced inflation and deflation of specifically-placed airbags, has become of increasing interest, as it has been found to alleviate PASC-associated anxiety by improving patient cardiovascular function. These functional improvements were achieved by EECP stimulating anti-inflammatory and pro-angiogenic processes, as well as improving endothelial cell function and coronary blood flow, partially via counteracting against the negative effects of SARS-CoV-2 infection on the renin-angiotensin-aldosterone system. Therefore, EECP could promote both psychosomatic and cardiac rehabilitation. Further research, though, is still needed to fully determine its benefits and mechanism of action.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241067"},"PeriodicalIF":1.7,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11716443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network pharmacological analysis and <i>in vitro</i> testing of the rutin effects on triple-negative breast cancer.","authors":"Cheng Chang, Ruiying Jia, Bin Fang, Yaoyao Miao, Lili Zhang","doi":"10.1515/med-2024-1079","DOIUrl":"10.1515/med-2024-1079","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to assess the potential mechanism of rutin to treat triple-negative breast cancer (TNBC) based on network pharmacology followed by <i>in vitro</i> experiments.</p><p><strong>Methods: </strong>The potential rutin targets were predicted, and the DisGeNET database was used to obtain the disease targets. The intersection targets were identified with Venny 2.1 software, with the String database subsequently used as input to produce the \"drug-target-disease\" visual network employing Cytoscape 3.7.2. Gene ontology. Kyoto Encyclopaedia of Genes and Genomes analyses were performed for intersection targets, while AutoDock Vina was used for molecular docking and visualization. Cell viability was assessed using the Colorimetric CCK-8 test, and apoptosis was analyzed using PI/Annexin V. The predicted core targets were confirmed by qPCR and western blotting assays.</p><p><strong>Results: </strong>EGFR, IL6, TNF, and INS were found as the primary targets. The molecular docking analysis revealed the rutin interaction with the core targets. The <i>in vitro</i> results confirmed that rutin inhibited the growth of the MDA-MB-231 cell line. Rutin also induced cell death and decreased the expressions of IL6, TNF, INS, and EGFR.</p><p><strong>Conclusion: </strong>Rutin's multi-target effects and molecular mechanism for treating TNBC were confirmed through preliminary results. The results provide a theoretical base for rutin's possible function in breast cancer treatment.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"20 1","pages":"20241079"},"PeriodicalIF":1.7,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11716441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in mortality risk by levels of physical activity among persons with disabilities in South Korea.","authors":"SeungCheor Lee, Yeowool Lee, Saengryeol Park, So-Youn Park, In-Hwan Oh","doi":"10.1515/med-2024-1118","DOIUrl":"10.1515/med-2024-1118","url":null,"abstract":"<p><strong>Aim: </strong>The World Health Organization's recommendation of at least 150 min of physical activity per week is important for increasing the lifespan of persons with disabilities (PWDs).</p><p><strong>Methods: </strong>Conduct a survival analysis to examine the relationship between physical activity and mortality using cohort data from the National Health Insurance Service in South Korea from 2017 to 2021. The survival analysis included 259,146 PWDs, with a maximum follow-up of 57 months, and adjustments for covariates, including physical activity level, comorbidities, smoking, and alcohol consumption.</p><p><strong>Results: </strong>People who exercised >150 min weekly had a lower risk of death compared to those who exercised less (adjusted hazard ratio [AHR]: 0.727, 95% confidence interval [CI]: 0.674-0.784). The risk of death increased with increasing age (AHR: 1.08, 95% CI: 1.077-1.083), smokers had a higher risk of death than non-smokers (AHR: 1.487, 95% CI: 1.396-1.583), and the risk of death increased with increasing Charlson comorbidity index scores (AHR: 1.228, 95% CI: 1.22-1.237).</p><p><strong>Conclusion: </strong>Even after adjusting for socioeconomic and other risk factors, PWDs who are physically inactive have a higher risk of death. Customized physical activity policies for PWDs are needed to reduce health inequities.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241118"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent opportunistic infections in a HIV-negative patient with combined C6 and <i>NFKB1</i> mutations: A case report, pedigree analysis, and literature review.","authors":"Yamei Zheng, Liwen Guan, Jiao Li, Yihui Fu","doi":"10.1515/med-2024-1019","DOIUrl":"10.1515/med-2024-1019","url":null,"abstract":"<p><strong>Introduction: </strong>Recurrent opportunistic infections are particularly common in patients infected with human immunodeficiency virus (HIV). However, these opportunistic infections have also been reported in HIV-negative patients, especially those with primary immunodeficiency disorder (PID), a condition that involves a large heterogeneous group of disorders arising from defects in immune system development and/or function.</p><p><strong>Case: </strong>Here, we report a very rare case of recurrent opportunistic infections in a non-HIV-infected patient combined with mutations in complement component C6 and nuclear factor kB subunit 1 (<i>NFKB1</i>). The patient first developed <i>Pneumocystis jirovecii</i> pneumonia, followed by cytomegalovirus esophagitis. Reduced CD4+ T and B lymphocyte counts, hypogammaglobulinemia were observed. The patient was HIV negative, and congenital immunodeficiency-related genes indicated combined C6 and <i>NFKB1</i> mutations. Gene detection was undertaken with blood samples from the patient's parents and younger brother. None of the family members possessed both gene mutations, suggesting that the simultaneous mutations of C6 and <i>NFKB1</i> caused primary immunodeficiency in the patient and resulted in recurrent opportunistic infections. In addition, we performed a review of the relevant literature to assess the clinical manifestations of C6 and <i>NFKB1</i> mutations.</p><p><strong>Conclusion: </strong>A diagnosis of PID should be suspected in patients with recurrent opportunistic infections, decreased CD4+ T and B lymphocyte, and hypoimmunoglobulinemia when secondary immunodeficiency factors can be excluded. In addition, genetic testing of family members should be performed, which may lead to the discovery of novel familial gene mutations.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241019"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143009411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of signatures associated with microsatellite instability and immune characteristics to predict the prognostic risk of colon cancer.","authors":"Sihan Bo, Yong You, Yongwei Wang, Yan Zhang, Bing Bai, Tao Jiang, Yaxian Gao","doi":"10.1515/med-2024-1056","DOIUrl":"10.1515/med-2024-1056","url":null,"abstract":"<p><strong>Background: </strong>Microsatellite instability (MSI) significantly impacts treatment response and outcomes in colon cancer; however, its underlying molecular mechanisms remain unclear. This study aimed to identify prognostic biomarkers by comparing MSI and microsatellite stability (MSS).</p><p><strong>Methods: </strong>Data from the GSE39582 dataset downloaded from the Gene Expression Omnibus database were analyzed for differentially expressed genes (DEGs) and immune cell infiltration between MSI and MSS. Then, weighted gene co-expression network analysis (WGCNA) was utilized to identify the key modules, and the modules related to immune infiltration phenotypes were considered as the immune-related gene modules, followed by enrichment analysis of immune-related module genes. Prognostic signatures were derived using Cox regression, and their correlation with immune features and clinical features was assessed, followed by a nomogram construction.</p><p><strong>Results: </strong>A total of 857 DEGs and 14 differential immune cell infiltration between MSI and MSS were obtained. Then, WGCNA identified two immune-related modules comprising 356 genes, namely MEturquoise and MEbrown. Eight signature genes were identified, namely <i>PLK2</i>, <i>VSIG4</i>, <i>LY75</i>, <i>GZMB</i>, <i>GAS1</i>, <i>LIPG</i>, <i>ANG</i>, and <i>AMACR</i>, followed by prognostic model construction. Both training and validation cohorts revealed that these eight signature genes have prognostic value, and the prognostic model showed superior predictive performance for colon cancer prognosis and distinguished the clinical characteristics of colon cancer patients. Notably, <i>VSIG4</i> among the signature genes correlated significantly with immune infiltration, human leukocyte antigen expression, and immune pathway enrichment. Finally, the constructed nomogram model could significantly predict the prognosis of colorectal cancer.</p><p><strong>Conclusion: </strong>This study identifies eight prognostic signature genes associated with MSI and immune infiltration in colon cancer, suggesting their potential for predicting prognostic risk.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241056"},"PeriodicalIF":1.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-cell analysis identified key macrophage subpopulations associated with atherosclerosis.","authors":"Zhenzhen Zhao, Yuelong Qin, Rui Wu, Wenwu Li, Yujiang Dong","doi":"10.1515/med-2024-1088","DOIUrl":"10.1515/med-2024-1088","url":null,"abstract":"<p><strong>Background: </strong>Atherosclerosis is a lipid-driven inflammatory disease characterized by plaque formation in major arteries. These plaques contain lipid-rich macrophages that accumulate through monocyte recruitment, local macrophage differentiation, and proliferation.</p><p><strong>Objective: </strong>We identify the macrophage subsets that are closely related to atherosclerosis and reveal the key pathways in the progression of atherosclerotic disease.</p><p><strong>Materials and methods: </strong>In this study, we characterize the single-cell landscape of atherosclerosis, identifying macrophage subsets closely related to the disease and revealing key pathways in its progression. Using analytical methods like CytoTRACE, Monocle2, Slingshot, and CellChat, we study macrophage differentiation and infer cell trajectory.</p><p><strong>Results: </strong>The 8,417 macrophages were divided into six subtypes, macrophages: C0 C1QC+ macrophages, C1 SPP1+ macrophages, C2 FCN1+ macrophages, C3 IGKC+ macrophages, C4 FCER1A+ macrophages, C5CALD1+ macrophages. The results of gene set enrichment analysis, Monocle2, and Slingshot suggest that C2 FCN1+ macrophages may play an important role in the progression of atherosclerosis. C2 FCN1+ macrophages interact with endothelial cells via CCL, CXCL, APP, and other pathways to regulate the progression of atherosclerosis.</p><p><strong>Conclusion: </strong>We identify a key macrophage subgroup (C2 FCN1+ macrophages) associated with atherosclerosis, which interacts with endothelial cells via CCL, CXCL, APP, and other pathways to regulate disease progression.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241088"},"PeriodicalIF":1.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhein promotes skin wound healing by activating the PI3K/AKT signaling pathway.","authors":"Dong Yang, Wei Li, Ping Xiang, Tingrui Ge, Huazhuan Li, Yonggang Zhang","doi":"10.1515/med-2024-1116","DOIUrl":"10.1515/med-2024-1116","url":null,"abstract":"<p><p>Rhein is a natural anthraquinone substance extracted from <i>Rheum palmatum</i> L. This study aimed to evaluate Rhein's protective effects against skin wound by <i>in vivo</i> and <i>in vitro</i> models and investigate whether its protective mechanism regulated the PI3K/AKT signaling pathway. The skin wound mice model was established and then treated with Rhein for 10 days. Hematoxylin and eosin staining and Masson's trichrome staining were applied to assess histological changes and collagen maturity in the mice skin wound tissues. Human skin fibroblasts (HSFs) viability, migration, and invasion were detected by Cell counting kit-8 (CCK-8), scratch wound, and transwell assays respectively. Moreover, the protein expression of p-PI3K, PI3K, p-AKT, and AKT were determined by western blot assay. We found that local treatment with Rhein promoted skin wound healing and accelerated collagen maturation, compared with the Model group. In addition, Rhein promoted skin wound healing through accelerated HSF proliferation, migration, and invasion. Furthermore, Rhein remarkably enhanced p-PI3K and p-AKT expression, as well as p-PI3K/PI3K and p-AKT/AKT ratio in skin wound mice and HSF cells, suggesting that Rhein promoted skin wound healing by activating PI3K/AKT signaling pathway. In conclusion, Rhein is a promising agent for promoting wound healing of skin tissues.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241116"},"PeriodicalIF":1.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Frankincense: A neuronutrient to approach Parkinson's disease treatment\".","authors":"Vittorio Calabrese, Naomi Osakabe, Foziya Khan, Uwe Wenzel, Sergio Modafferi, Lidia Nicolosi, Tilman Fritsch, Ursula M Jacob, Ali S Abdelhameed, Luay Rashan","doi":"10.1515/med-2024-2001","DOIUrl":"https://doi.org/10.1515/med-2024-2001","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1515/med-2024-0988.].</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20242001"},"PeriodicalIF":1.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Investigating hormesis, aging, and neurodegeneration: From bench to clinics\".","authors":"Vittorio Calabrese, Uwe Wenzel, Tommaso Piccoli, Ursula M Jacob, Lidia Nicolosi, Giovanni Fazzolari, Gabriella Failla, Tilman Fritsch, Naomi Osakabe, Edward J Calabrese","doi":"10.1515/med-2024-2000","DOIUrl":"https://doi.org/10.1515/med-2024-2000","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1515/med-2024-0986.].</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20242000"},"PeriodicalIF":1.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of serum FOXM1 and IGF2 in patients with ARDS and their correlation with disease and prognosis.","authors":"Chao Liu, Shengrui Zhang, Weiwei Zhang, Jinfeng Wang","doi":"10.1515/med-2024-1093","DOIUrl":"10.1515/med-2024-1093","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the relationship between the expression levels of serum forkhead box protein M1(FOXM1) and insulin-like growth factor 2 (IGF2) mRNA in patients with acute respiratory distress syndrome (ARDS) condition and prognosis.</p><p><strong>Methods: </strong>Ninety patients with ARDS admitted to our hospital were regarded as the ARDS group, according to the prognosis, they were grouped into death group (<i>n</i> = 64) and survival group (<i>n</i> = 126); the control group consisted of 190 healthy individuals.</p><p><strong>Results: </strong>Compared with the control group, the level of serum FOXM1 mRNA in ARDS group was obviously lower, and the level of IGF2 mRNA was higher. The serum IGF2 mRNA, serum creatinine, inhaled oxygen concentration (FiO₂), and mechanical ventilation time in the death group were higher than those in the control group, and the arterial oxygen partial pressure (PaO₂), FOXM1 mRNA, and oxygenation index (PaO₂/FiO₂) were lower than those in control group. Logistic regression analysis indicated that FOXM1, IGF2, and PaO₂/FiO₂ were significant factors influencing the prognosis and mortality in ARDS patients. Correlation analysis showed that there was a negative correlation between serum FOXM1 and IGF2 mRNA levels in patients with ARDS.</p><p><strong>Conclusion: </strong>Serum FOXM1 and IGF2 mRNA in patients with ARDS are correlated with the severity and prognosis of ARDS.</p>","PeriodicalId":19715,"journal":{"name":"Open Medicine","volume":"19 1","pages":"20241093"},"PeriodicalIF":1.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}