2016 32nd Southern Biomedical Engineering Conference (SBEC)最新文献_第2页

Strategies for Creating Cardiovascular Models with Digital Light Projection Stereolithography 用数字光投影立体光刻技术创建心血管模型的策略

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.53

J. T. Frank, W. Frank, Charles E. Taylor

{"title":"Strategies for Creating Cardiovascular Models with Digital Light Projection Stereolithography","authors":"J. T. Frank, W. Frank, Charles E. Taylor","doi":"10.1109/SBEC.2016.53","DOIUrl":"https://doi.org/10.1109/SBEC.2016.53","url":null,"abstract":"Stereolithography (SLA) represents an additive manufacturing method that uses light to selectively cure a photoresin for the purpose of creating a 3 dimensional structure. With successively cured layers, large geometry (4\" x 6\" x 15\") can be achieved with incredibly high resolution (~100 micrometer). Using a digital light projection (DLP) chip, whole layers of the model can be cured in a single step as opposed to laser based methods that require the laser to line scan the model. This approach rapidly accelerates the printing process, while still maintaining a high level of accuracy in the part. The goal of this printer is to produce models of the cardiovascular system for benchtop flow studies. With many of the intended models being complex in nature, fused filament printing would not produce the needed resolution or finish required. The challenge with many models is how segment the model for printing, curing settings, and how to position the geometry on the print bed. Different photoresin chemistry's were used achieve specific model objectives, reusable molds, dissolvable molds, and device prototypes. The underlying chemistry, printing strategies, and discussion of the tested solutions to problems with this technology will be covered. With the growing presence of DLP SLA, many of the challenges with this method are limiting the success of these designs in the marketplace. This presentation will cover the advances made with the current design and the benefits this technology has for this application.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133262517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scn2a-Null Heterozygosity Improves Survival and Modifies Neurocardiac Interaction in the Kcna1-Null Mouse Model of SUDEP 在Kcna1-Null小鼠模型中，Scn2a-Null杂合性改善生存并改变神经心脏相互作用

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.47

V. Mishra, Nicky Gautier, Bharat Karumuri, Rui Liu, I. Vlachos, L. Iasemidis, E. Glasscock

{"title":"Scn2a-Null Heterozygosity Improves Survival and Modifies Neurocardiac Interaction in the Kcna1-Null Mouse Model of SUDEP","authors":"V. Mishra, Nicky Gautier, Bharat Karumuri, Rui Liu, I. Vlachos, L. Iasemidis, E. Glasscock","doi":"10.1109/SBEC.2016.47","DOIUrl":"https://doi.org/10.1109/SBEC.2016.47","url":null,"abstract":"Sudden unexpected death in epilepsy (SUDEP) is the leading cause of epilepsy-related mortality, but its genetic etiology is largely unknown and likely complex involving multiple genes. The Kcna1 gene encodes Kv1.1 potassium channels that act to dampen neuronal excitability whereas the Scn2a gene encodes Nav1.2 sodium channels important for action potential conduction. We tested the hypothesis that subclinical Scn2a heterozygosity reduces SUDEP incidence in Kcna1-null mice of SUDEP, by suppressing neurocardiac dysfunction associated with the absence of Kv1.1 channels. SUDEP-prone Kcna1-null mice exhibited about a 50% increase in lifespan when heterozygous for the Scn2a-null allele, that is Scn2a+/-, Kcna1-/-. In simultaneous video electroencephalography (EEG)- electrocardiography (ECG) recordings, Scn2a heterozygosity did not eliminate seizures in Kcna1-/- mice, but seizure burden was partially reduced due to a significant reduction in seizure durations. Analysis of beat-to-beat heart rate variability (HRV) revealed that Kcna1-/- and Scn2a+/-, Kcna1-/- mice exhibit similarly high RMSSD (Root Mean Square of the Successive Differences), suggesting the functional interactions between the two mutations do not involve significant alteration of parasympathetic tone. Moreover, analysis of EEG-ECG interaction dynamics revealed a significantly higher degree of association between brain and cardiac activity in double mutant mice compared to Kcna1-/- animals for several different measures, suggesting that Scn2a-null heterozygosity reduces SUDEP risk by altering neural control of the heart. These findings expand our understanding of the complex genetic interactions underlying SUDEP and identify EEG-ECG association as a potential new biomarker of SUDEP susceptibility.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124158261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DRAQ5 and Eosin as a Topical Fluorescent Analogue for H&E in Digital Pathology DRAQ5和伊红作为数字病理中H&E的局部荧光类似物

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.82

Katherine Elfer, Andrew B. Sholl, Mei Wang, J. Q. Brown

引用次数: 0

Early Detection of Focal Seizures in Thalamus Using a Novel Seizure Detection Paradigm: Toward a Closed-Loop Thalamic Stimulation 使用一种新的癫痫检测范式早期检测丘脑局灶性癫痫:朝着闭环丘脑刺激

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.78

D. Pizarro, K. Majumdar, S. Pati

{"title":"Early Detection of Focal Seizures in Thalamus Using a Novel Seizure Detection Paradigm: Toward a Closed-Loop Thalamic Stimulation","authors":"D. Pizarro, K. Majumdar, S. Pati","doi":"10.1109/SBEC.2016.78","DOIUrl":"https://doi.org/10.1109/SBEC.2016.78","url":null,"abstract":"Despite therapeutic advances, many persons with epilepsy may not be candidates for resective surgery or RNS device if the seizure is non-localizable. One potential approach to treating epilepsy in this cohort is to provide stimulation to thalamus. One of the pre-requisite for developing an effective closed loop therapy is early detection of seizure. Here we report our experience in mapping the temporal dynamics of thalamic activity during transition from inter-ictal to seizures and compare a novel seizure detection method called \"P-operator\" to Curve length. Data was recorded from five adults with history of difficult-to-localize epilepsies using stereo-depth electrodes implanted in thalamus. Matlab was used to analyze power spectrum, power spectral density, comparison between P operator against curve length for substantiating functional connectivity between the seizure onset zone(s) and thalamus. A total of 22 seizures were recorded and selected for analysis. Matlab analysis with P-operator and curve length confirmed involvement of thalamus in 4 out of 5 patients. Curve length detected seizure earlier that P operator, however P-operator showed more efficacy for detection and count of interictals. Spectral analysis confirmed cortico-thalamic involvement between 100-400 Hz and the seizures were detected in thalamus within -10 to +5 seconds from cortical onset (T=0 sec). Thalamic signatures at seizure onset differed from offset. Two seizure detection methods (curve length and P-operator) gave similar results. Curve length had an average percentage of error of 5% compared to 9.6% for P operator. Thalamus was involved early in patients with intractable focal epilepsies.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116819493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Classification of Ocular Disorders Based on Fractal and Invariant Moment Analysis of Retinal Fundus Images 基于视网膜眼底图像分形和不变矩分析的眼部疾病分类

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.21

Noah Hutson, Anik Karan, J. Adkinson, P. Sidiropoulos, I. Vlachos, L. Iasemidis

引用次数: 4

Effects of Strap Options on Scoliosis Bracing Mechanics: An Application of the Scoliosis Analog Model 绑带选择对脊柱侧凸支撑力学的影响:脊柱侧凸模拟模型的应用

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.37

C. Chung, D. Kelly, Jack R Steele, D. DiAngelo

{"title":"Effects of Strap Options on Scoliosis Bracing Mechanics: An Application of the Scoliosis Analog Model","authors":"C. Chung, D. Kelly, Jack R Steele, D. DiAngelo","doi":"10.1109/SBEC.2016.37","DOIUrl":"https://doi.org/10.1109/SBEC.2016.37","url":null,"abstract":"Summary form only given. Thoracolumbar braces are commonly used to treat Adolescent Idiopathic Scoliosis (AIS). Braces serve to reduce and prevent progression of the spinal curve by applying corrective forces, which are affected by alterations made during the brace fitting process. The Scoliosis Analog Model (SAM), a mechanical equivalent of the AIS condition capable of simulating up to 40 degrees of spinal correction, enables analysis of corrective forces applied by the brace to the spine and structural properties of the brace. This model was used to investigate the effects of strap-related brace alterations. A Boston brace was tested using the SAM and biorobotic testing platform. Various configurations of Velcro and buckle straps used to fasten the brace were tested and compared to unconstrained and rigidly-constrained configurations. Measurements of the force components applied to the model and SAM angular displacement were used to calculate brace structural stiffness properties. Addition of either strap type significantly increased the stiffness values relative to the unconstrained brace configuration. An optimal brace radial stiffness was achieved with three Velcro straps, i.e., there was no significant stiffness gained by adding a fourth strap. For the case of the buckle straps, no significant stiffness gain occurred when more buckle straps were added. Structural properties provide a means to compare bracing technology and better understand design alterations. A measurable difference exists in brace force response and structural properties between each configuration. Interpretation of the measured force components revealed that the brace applied inward and upward forces to the spine.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121449761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Bioengineering, Science Fiction, and Medical Ethics: How Goes the Flow? 生物工程，科幻小说和医学伦理:如何流动?

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.94

Anne Hudson Jones

{"title":"Bioengineering, Science Fiction, and Medical Ethics: How Goes the Flow?","authors":"Anne Hudson Jones","doi":"10.1109/SBEC.2016.94","DOIUrl":"https://doi.org/10.1109/SBEC.2016.94","url":null,"abstract":"Because of their ability to foresee the future, artists and writers have famously been called \"the antennae of the race\" by 20th-century poet Ezra Pound. Whether writers, especially those described as science-fiction (SF) writers, inspire bioengineers with their depictions of imaginary futures or whether bioengineers with their inventions inspire SF writers may not always be clear. However goes the flow of ideas, the efforts of SF writers often point to new and challenging issues in medical ethics before others have identified them. In this short presentation, I will give examples of ways in which SF writers see the future concerns and ethical dilemmas that will be created by breakthrough cybernetic technologies such as cardiac pacemakers, artificial replacement organs, and implanted computer chips. The triumphs of bioengineering can lead from relatively straightforward ethical questions, such as whether bioengineered devices should be turned off or removed and, if so, in what circumstances, to profound philosophical questions about what it means to be human and how much human body can be mechanically altered without ushering in transhumanist and posthumanist futures. In concluding, I will consider how SF stories might best be used in the education of bioengineers to help alert them to the far-reaching philosophical and ethical dimensions of their work.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116258426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Mild Traumatic Brain Injury on Auditory Function in a Mouse Model 轻度创伤性脑损伤对小鼠听觉功能的影响

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.48

R. Amanipour, S. Cresoe, C. Borlongan, R. Frisina, J. Walton

{"title":"Effects of Mild Traumatic Brain Injury on Auditory Function in a Mouse Model","authors":"R. Amanipour, S. Cresoe, C. Borlongan, R. Frisina, J. Walton","doi":"10.1109/SBEC.2016.48","DOIUrl":"https://doi.org/10.1109/SBEC.2016.48","url":null,"abstract":"Traumatic brain injury negatively impacts auditory processing including difficulty in hearing in background noise. In this study we used behavioral and electrophysiological outcomes to examine the effects of mTBI on perceptual auditory disorders and change in behavioral responses overtime following injury. Subjects were 16 (equal gender), 6 month old CBA/CaJ mice. All experimental protocols were approved by the University of South Florida Institutional Animal Care and Use Committee (IACUC). Mice were exposed to either sham or to mTBI administered using a controlled cortical impactor. Auditory behavioral responses were measured using acoustic startle response (ASR). Auditory brainstem response (ABR) audiograms from 8 to 32 kHz were used to measure hearing function. A gap-in-noise paradigm (70 dB SPL markers with gap durations of 2 to 64 ms) was also used to assess temporal processing. All TBI animals showed symptoms immediately following TBI: motor lethargy, poor appetite and mild weight loss, with recovery seen within 3 days. Behavioral and electrophysiological assessments were completed at 3, 7, 14, 28, 35, 45, and 90 days post-TBI. Three days post-TBI, ASR functions were comparable between TBI and sham groups. For ABRs, there was a significant decrease in the amplitudes of P1 (22%) and P4 (30%), and an increase in P4 latency in the mTBI mice. These findings indicate that mTBI can result in significant long term deficits of auditory processing and the mouse model may prove to be a translational model for understanding the pathology and treatment of mTBI-induced auditory dysfunction.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131147133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Liquid Property Control System for LVAD ISO 5198 Testing and Mock Circulatory Loop Simulations LVAD液性控制系统ISO 5198测试及模拟循环回路仿真

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.62

J. Friedberg, M. L. Chavez, Joseph Young, J. Daigle, Charles E. Taylor

{"title":"Liquid Property Control System for LVAD ISO 5198 Testing and Mock Circulatory Loop Simulations","authors":"J. Friedberg, M. L. Chavez, Joseph Young, J. Daigle, Charles E. Taylor","doi":"10.1109/SBEC.2016.62","DOIUrl":"https://doi.org/10.1109/SBEC.2016.62","url":null,"abstract":"Determination of performance for fluid power systems through a range of operating conditions is a crucial aspect of safety assurance. Experimental confirmation supports the computational design and verification steps taken to ensure the mechanism will perform adequately. Pumps, valves and other devices destined for hydraulic can have their performance be subject to differences in the fluid properties of the liquid. This necessitates that a range of fluid properties be examined to ensure that the operating envelope is properly characterized. Past approaches to these experiments have involved modifying the system fluid manually and only investigating a sparse set of conditions. Proposed is a liquid property unit that is able to make changes to a system fluid of a bench testing apparatus used to evaluate hydraulic devices. The chosen fluid system for the initial release of this design is a water:glycerin composition. The intent is to have a fluid chemistry that has a broad range of densities and viscosities. This unit is controlled via a user interface and is able to execute set point changes to the system fluid and maintain the total system volume desired. The design of the system, validation of its operation and use of the fluid in exemplary trials will be covered.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131768858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wireless Optogenetic Manipulation of Direct-Pathway Neurons of Basal Ganglia in Free Moving Mice 自由运动小鼠基底神经节直接通路神经元的无线光遗传操作

2016 32nd Southern Biomedical Engineering Conference (SBEC) Pub Date : 2016-03-11 DOI: 10.1109/SBEC.2016.52

Xinli Tian, Adam D. Richard, Isabella Van Savage, Xiao-Hong Lu

{"title":"Wireless Optogenetic Manipulation of Direct-Pathway Neurons of Basal Ganglia in Free Moving Mice","authors":"Xinli Tian, Adam D. Richard, Isabella Van Savage, Xiao-Hong Lu","doi":"10.1109/SBEC.2016.52","DOIUrl":"https://doi.org/10.1109/SBEC.2016.52","url":null,"abstract":"Striatum is one of the major components of basal ganglia. More than 90% of principle neurons in striatum are medium spiny neuron (MSN). The MSNs are further subdivided into the direct-pathway (striatonigral or D1) MSNs or the indirect-pathway (striatopallidal or D2) MSNs. The striatonigral MSNs project to GPi and substantia nigra (SN). The striatopallidal MSNs project to globus pallidus externa (GPe), which can indirectly influence the SN via the subthalamic nuclei (STN). Disruption of two pathway have been implicated in the pathogenesis of neuropsychiatric disorders such as Parkinson's disease, Huntington's disease, Drug addiction and Schizophrenia. The development of optogenetics has introduced an elegant method to stimulate MSNs and basal ganglia circuits, both in vitro and in vivo. However, current optogenetics setup employs cumbersome tethers and commutators that prohibits the studying the free, natural behavior of the animal. Using a light weight (1-2 g) wireless infrared receiver coupled to optic fiber implants, we could deliver mW order light in free mouse. Injection of Cre-dependent Channelrhodopsin or/and halorhodops in an inverted open-reading-frame in Bacterial Artificial Chromosome (BAC) transgenic Drd1a-Cre mice enables us to precisely stimulate or/and inhibit genetically defined direct-pathway neurons. We have successfully employed such optogenetic device to examine more complex behavior in plus maze (for anxiety), three chamber interaction test (for social interaction) and rotarod tests. Our results revealed that direct-pathway stimulation elicits robust grooming behavior in free moving mice in their home cage, recapitulating the D1 receptor agonist-induced behavioral phenotypes in mice. These experiments demonstrate that the wireless optogenetic device can be readily used in complex behavioral experiments. Such technology will accelerate progress in both basic neuroscience and translational technologies and have strong potential for broader use in biology and medicine.","PeriodicalId":196856,"journal":{"name":"2016 32nd Southern Biomedical Engineering Conference (SBEC)","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133131793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0