DRAQ5 and Eosin as a Topical Fluorescent Analogue for H&E in Digital Pathology

Abstract

A novel, dual-stain fluorescent analogue to H&E, applied directly to both previously fixed and frozen tissue, is demonstrated. DRAQ5 and Eosin Y (D&E), when applied to human tissue, imaged using a fluorescent microscope, and pseudocolored to match H&E, visualizes morphological features that a pathologist found to directly correlate with gold standard bright field H&E.