Katherine Elfer, Andrew B. Sholl, Mei Wang, J. Q. Brown
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引用次数: 0
摘要
一种新的,双染色荧光模拟H&E,直接应用于以前固定和冷冻组织,被证明。当DRAQ5和Eosin Y (D&E)应用于人体组织时,使用荧光显微镜成像,并假色以匹配H&E,病理学家发现与金标准亮场H&E直接相关的形态学特征可视化。
DRAQ5 and Eosin as a Topical Fluorescent Analogue for H&E in Digital Pathology
A novel, dual-stain fluorescent analogue to H&E, applied directly to both previously fixed and frozen tissue, is demonstrated. DRAQ5 and Eosin Y (D&E), when applied to human tissue, imaged using a fluorescent microscope, and pseudocolored to match H&E, visualizes morphological features that a pathologist found to directly correlate with gold standard bright field H&E.