Ontogenez最新文献_第7页

[Thymus Development in Early Ontogeny: A Comparative Aspect]. [早期个体发育中胸腺发育的比较研究]。

Ontogenez Pub Date : 2015-05-01

K A Vasil'ev, A V Polevshchikov

引用次数: 0

[Hormonal Induction of In Vitro Maturation and Ovulation of Loach Oocytes and Obtaining Egg Cells Capable of Fertilization and Development]. [激素诱导泥鳅卵母细胞体外成熟和排卵及获得能够受精发育的卵细胞]。

Ontogenez Pub Date : 2015-05-01

M N Skoblina, A A Minin

引用次数: 0

[Epigenetic mutagenesis as program of age-related protein dysfunction and aging]. [表观遗传突变作为与年龄相关的蛋白质功能障碍和衰老的程序]。

Ontogenez Pub Date : 2015-03-01

G A Romanov, V S Sukhoverov, B F Vanyushin

{"title":"[Epigenetic mutagenesis as program of age-related protein dysfunction and aging].","authors":"G A Romanov, V S Sukhoverov, B F Vanyushin","doi":"","DOIUrl":"","url":null,"abstract":"DNA methylation plays an important polyfunctional role in ontogenesis of human and mammals. A steep rise in probability of mutational substitution of CpG dinucleotide on TpG dinucleotide in the genome is one of the consequences of DNA methylation. All spectrum (17) of possible DNA and protein mutations caused by CpG-dinucleotide methylation in DNA were characterized, and the three most dangerous mutations (able to result in protein inactivation) were isolated. The computer program that allows one to predict all most probable mutations in the analyzed gene and encoded protein was created. On the example of genes from humans and various mammals, it was demonstrated that the amount of potentially dangerous sites of epigenetic mutagenesis in exons was drastically decreased as a result of genome evolution. But, at the same time, unforced preservation of such sites and their persistence were established, indicating the occurrence of age-related protein dysfunction built into the genome epigenetic program, resulting in apoptosis and aging; this program is based on the set and position of methylated codons in exonic gene regions. It is assumed that the program of epigenetic mutagenesis limits the lifetime of an individual, accelerating the deliverance of the population from long-lived individuals that completed the reproductive period.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 2","pages":"102-13"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33216359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Some molecular and genetic properties of progenitor cells in sarcomas induced with foreign body]. [异物诱导肉瘤中祖细胞的一些分子和遗传特性]。

Ontogenez Pub Date : 2015-03-01

O V Morozova, A F Karamysheva, T G Moizhess

引用次数: 0

[Caspar Friedrich Wolff: the emergence of epigenesis]. [卡斯帕·弗里德里希·沃尔夫:后生的出现]。

Ontogenez Pub Date : 2015-03-01

E B Muzrukova, M A Pomelova

引用次数: 0

[Embryos and gametes cryopreservation for genetic resources conservation of laboratory animals]. [实验动物胚胎配子低温保存的遗传资源保护]。

Ontogenez Pub Date : 2015-03-01

S Ya Amstislavsky, E Yu Brusentsev, K A Okotrub, I N Rozhkova

引用次数: 0

[The role of ethylene in activation division cell quiescent center in the cut maize roots]. [乙烯在玉米切根分裂细胞静止中心激活中的作用]。

Ontogenez Pub Date : 2015-03-01

E I Bystrova, N V Zhukovskaya, V J Rakitin, V B Ivanov

引用次数: 0

[Developmental abnormalities in salmonids (Salmonidae) under the conditions of large-scale volcanic pollution of their spawning ground (using dolly varden Salvelinus malma as an example)]. [产卵地大规模火山污染条件下鲑科(鲑科)的发育异常(以多利varden Salvelinus malma为例)]。

Ontogenez Pub Date : 2015-03-01

E V Esin

{"title":"[Developmental abnormalities in salmonids (Salmonidae) under the conditions of large-scale volcanic pollution of their spawning ground (using dolly varden Salvelinus malma as an example)].","authors":"E V Esin","doi":"","DOIUrl":"","url":null,"abstract":"Rivers originating from the areas of active volcanism in Kamchatka serve a spawning ground for anadromous and resident populations of dolly varden (Salvelinus malma). In some cases, watercourses with a long-term continuous spawning of S. malma are subjected to chronic pollution with dissolved toxicants and suspended mineral particles. The revealed development conditions range from background (\"clean\" rivers) to critical (most \"polluted\" rivers). Medium pollution leads to the development of hatchlings with abnormalities in the ethmoidal head segment, lower jaw, operculum, lobes of the paired fins, and axial skeleton (up to 40% of all specimens). Delayed ossification of skeletal elements takes place. Abnormalities in the development of spinous processes occur more often (up to 49-55% compared to 25-30% in the background areas). The average number of asymmetries per specimen.(in four bilateral structures) increases from 1.1-1.4 to 1.7- 2.5. Similar developmental abnormalities have been registered in underyearlings, both anadromous and resident, influenced by various pollutant combinations. While fish continue to grow, some of them die because of abnormalities; thus, their frequency in 3-year-old specimens nears the background one. Upon extreme pollution, deviant specimens are sampled at earlier developmental stages and characterized by a lower frequency of morphological abnormalities.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 2","pages":"114-25"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33216358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Sex identification of the rainbow trout Oncorhynchus mykiss by polymerase chain reaction]. 用聚合酶链反应鉴定虹鳟性别[j]。

Ontogenez Pub Date : 2015-03-01

Yu P Rud, M I Maistrenko, L P Buchatskii

{"title":"[Sex identification of the rainbow trout Oncorhynchus mykiss by polymerase chain reaction].","authors":"Yu P Rud, M I Maistrenko, L P Buchatskii","doi":"","DOIUrl":"","url":null,"abstract":"A polymerase chain reaction-based test for rapid sex identification of the rainbow trout Oncorhynchus mykiss has been elaborated. Using the data deposited with the NCBI genome bank, nucleotide sequences of the specific sex-linked locus of salmonid species have been analyzed and the oligonucleotide primers have been selected. The length of the amplification products is 800 nucleotide pairs. Amplification specificity has been tested by nucleotide analysis of the amplicon sequences. All PCR products match the Y chromosome region housing the corresponding specific locus. A comparative analysis of the nucleotide sequences of the fragments carrying the rainbow trout (and other salmonid species) sex-linked marker demonstrates a high degree of identity, amounting to 95-99%. The maximal identity between the sequences (99%) is observed when analyzing representatives of the same genus, for example, Oncorhynchus. Thus, the sex is detectable by a simple PCR test, thereby allowing the male revertants emerging via hormonal sex reversion to be identified. This test is a rapid diagnostic method, since it requires only 1 day for testing and informing the fish farm on its results. Results of abdomen opening of rainbow trout revertants and examination of their hormonal status are also described.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 2","pages":"87-93"},"PeriodicalIF":0.0,"publicationDate":"2015-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33343175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions]. [阻断蛋白酶体活性扰乱了细胞与细胞直接相互作用的蛋白质标记物的合成节奏]。

Ontogenez Pub Date : 2015-01-01

V Ia Brodskiĭ, N P Sharova, L A Mal'chenko, D S Konchenko, T K Dubovaia, N D Zvezdina

{"title":"[Blockade of proteasome activity disturbs the rhythm of synthesis of the protein marker of direct cell-cell interactions].","authors":"V Ia Brodskiĭ, N P Sharova, L A Mal'chenko, D S Konchenko, T K Dubovaia, N D Zvezdina","doi":"","DOIUrl":"","url":null,"abstract":"The effect of inhibition of proteasome activity on direct cell-cell interactions in primary hepatocyte cultures was studied. The circahoralian rhythm of protein synthesis was a marker of cell-cell communication. The addition of the proteasome inhibitor MG132 at doses of 10 or 20 μM to the medium with hepatocyte cultures for 19 h resulted in a significant reduction in the total pool of 3H-leucine in cells. The incorporation of leucine into proteins changed slightly or negligibly, whereas the content of free labeled leucine in hepatocytes decreased. The rhythm of protein synthesis was distorted compared to the control. The rhythm was restored by external organizers, such as gangliosides and melatonin, as well as by enhancing the activity of protein kinases--the key factor in the organization of the rhythm of protein synthesis. A short-term (3-h) exposure to MG132 did not change the pool of leucine, but the rhythm of protein synthesis was also disturbed. Thus, protein catabolism affects cell-cell interactions organizing the rhythm of protein synthesis. Another factor of the downregulation of the rhythm of protein synthesis, the secretion of proteins from the hepatocytes in vivo, which was shown in vivo in many studies, was also revealed in our study when measuring the content of proteins stained with Coomassie Brilliant Blue G250 in the medium with hepatocyte cultures.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 1","pages":"44-52"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33119695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0