Ontogenez最新文献_第6页

[Early Stages of Skull Embryogenesis in the Grass Snake, Natrix natrix (Serpentes, Colubridae)]. [草蛇，Natrix Natrix(蛇形目，蛇科)颅骨胚胎发生的早期阶段]。

Ontogenez Pub Date : 2015-07-01

M F Kovtun, H V Sheverdyukova

引用次数: 0

[Morphometry in Development of Red Deer's Adrenal Glands]. [马鹿肾上腺发育的形态计量学]。

Ontogenez Pub Date : 2015-07-01

N D Ovcharenko, O G Gribanova, L A Bondyreva

引用次数: 0

[In vitro Regeneration from Bulbous Scales of Fritillaria sonnikovae, an Endemic Species]. [特有种贝母幼鳞片离体再生研究]。

Ontogenez Pub Date : 2015-07-01

D S Kulkhanova, A A Erst, T I Novikova

引用次数: 0

[Morphogenesis of Human Fetal Thymus during Weeks 22-27 of Development]. [22-27周人胎儿胸腺的形态发育]。

Ontogenez Pub Date : 2015-07-01

L V Kulida, L P Peretyatko, S B Nazarov

引用次数: 0

[Extrathymic Differentiation of αβT Lymphocytes]. [αβT淋巴细胞胸腺外分化]。

Ontogenez Pub Date : 2015-07-01

E M Kuklina, N S Glebezdina

引用次数: 0

[Analysis of the Localization of Fibrillarin and Sites of Pre-rRNA Synthesis in the Nucleolus-Like Bodies of Mouse GV Oocytes after Mild Treatment with Proteinase K]. [蛋白酶K温和处理小鼠GV卵母细胞核仁样小体纤维蛋白定位及Pre-rRNA合成位点分析]。

Ontogenez Pub Date : 2015-05-01

K V Shishova, Yu M Khodarovich, E A Lavrentyeva, O V Zatsepina

{"title":"[Analysis of the Localization of Fibrillarin and Sites of Pre-rRNA Synthesis in the Nucleolus-Like Bodies of Mouse GV Oocytes after Mild Treatment with Proteinase K].","authors":"K V Shishova, Yu M Khodarovich, E A Lavrentyeva, O V Zatsepina","doi":"","DOIUrl":"","url":null,"abstract":"Postnatal development of mammalian oocytes is accompanied by functional and structural remodeling of the nucleolar apparatus: the final stage of this process is the formation of large objects (up to 10 μm in diameter) termed nucleolus-like bodies (NLBs) in preovulatory GV oocytes. N LB material was shown to be essential for early embryonic development, but its composition is still uncharacterized. In the present study, the protein-binding dye fluorescein-5-isothiocyanate (FITC) was used to show that proteins characterized by a high local concentration are essential NLB components in mouse GV oocytes. One of these proteins was able to be identified for the first time using a mild treatment of oocytes with proteinase K; the protein identified was fibrillarin, a factor of early pre-rRNA processing. Fibrillarin is present in the inner NLB mass of all oocytes capable of synthesizing rRNA; however, it is not colocalized with BrUTP microinjected into oocytes in order to identify transcribed ribosomal genes, in contrast to the \"surface\" fibrillarin. These observations imply the accumulation of nucleolar proteins not involved in ribosome biogenesis inside the NLB. All NLBs present in an individual nucleus of an NSN-type GV oocyte contain fibrillarin and are associated with active ribosomal genes. The results obtained in the present work demonstrate that proteinase K treatment of GV mouse oocytes allows for: (1) identification of \"cryptic\" proteins inside the densely packed NLB material and (2) the enhancement of oocyte image quality during BrUTP-based identification of rRNA synthesis sites but (3) not for the detection of active ribosomal genes in the inner mass of the NLB. The fluorescent dye FITC can be recommended for assessment of intracellular protein localization in the oocytes of all mammalian species.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 3","pages":"162-73"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34032671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Mechanisms Determining Bristle Pattern in Drosophila melanogaster]. 黑腹果蝇刚毛模式的决定机制

Ontogenez Pub Date : 2015-05-01

T A Bukharina, D P Furman

引用次数: 0

[Development of the Human Olfactory Bulbs in the Prenatal Ontogenesis: an Immunochistochemical Study with Markers of Presynaptic Terminals (anti-SNAP-25, -Synapsin-I, -Synaptophysin)]. [产前个体发育中人类嗅球的发育:突触前终末标记物(抗snap -25， - synapsin - 1， -Synaptophysin)的免疫组化研究]。

Ontogenez Pub Date : 2015-05-01

A S Kharlamova, V M Barabanov, S V Saveliev

{"title":"[Development of the Human Olfactory Bulbs in the Prenatal Ontogenesis: an Immunochistochemical Study with Markers of Presynaptic Terminals (anti-SNAP-25, -Synapsin-I, -Synaptophysin)].","authors":"A S Kharlamova, V M Barabanov, S V Saveliev","doi":"","DOIUrl":"","url":null,"abstract":"We provide the data of the olfactory bulbs (OB) development in the human fetuses on the stages from 8 week to birth. Immunochistochemical markers of presynaptic terminals (anti-SNAP-25, -synapsin-I, -synaptophysin) were used to evaluate the maturation of the OB. Differentiation of the OB layers begins from periphery, which implicitly evidences that growth of the olfactory nerves fibers induses not only anatomical differentiation of the OB, but also differentiation of its functional layers. The sites of the developing glomerulus are revealed using the immunochistochemical prosedure on the stage before distinct glomerulus can be identified with common histological procedure. OB conductive system demonstrates immunoreactivity with the antibodies to the presynaptic proteins on the all stages from 10-11 weeks of fetus development. Four stages of the OB development are described. All functional layers of the OB are mature at the 22-weeks stage. Further differentiation of the OB neuroblasts, including lamina formation of the internal granular leyer, glomerular layer development, OB growth continue after 20-22 weeks stage until 38-40 weeks of the fetus develoment. Patterns of the immunoreactivity with antibodies to SNAP-25, synapsin-I and synaptophysin are completely appropriate to those of adult's OB on the 38-40 weeks of the prenatal development. Complete maturity of the human OB is achived at 38-40 weeks of the prenatal development.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 3","pages":"174-85"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34032672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Articulata and Ecdysozoa]. [Articulata and Ecdysozoa].

Ontogenez Pub Date : 2015-05-01

O M Ivanova-Kazas

{"title":"[Articulata and Ecdysozoa].","authors":"O M Ivanova-Kazas","doi":"","DOIUrl":"","url":null,"abstract":"Science has accumulated to date such amounts of valuable and diverse information that no scientists can be encyclopedists (like those of the 17th and 18th centuries). Now every scientist is usually well informed only in one particular area and often needs consultations of other specialists. The current situation in biology is similar. In addition to evolutionary morphology, which represents fundamentals of zoology, there is a new, clearly cutting-edge and progressive area of studies, molecular genetics, which has already revealed many important general biological patterns. But the conclusions that follow from examining natural phenomena from this new point of view using new methods sometimes prove to be at odds with conventional notions. Considerable controversies have emerged on the phylogenetic position of the type Arthropoda. The peculiar features of the general body plan and the type of development of these animals seem to give evidence that they evolved from Annelida, with which they are often combined under the name Articulata. But attempts have been made to replace this concept by the idea that the clade Ecdysozoa, which includes Arthropods as well as such animals with low levels of organization as Nematoda and Priapulida, emerged early in the evolution of Bilateria. The main reason for combining the said animals in this clade is the fact that they have molts regulated with ecdysone; this point of view is supported by molecular genetic arguments. Although in this review this controversial problem is considered from the morphological point of view, the main purpose of the review is to emphasize the need to establish mutual understanding.between morphologists and molecular biologists and carefully find out the causes of the existing disagreements. Rather than ideological opponents, the two areas of science should be allies helping each other to solve complicated problems.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 3","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34032670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of Cancer-Testis Antigens of Magea and Mageb Families in Mouse Embryonic Fibroblasts Cultured in vitro]. [Magea和Mageb家族癌睾丸抗原在体外培养小鼠胚胎成纤维细胞中的表达]。

Ontogenez Pub Date : 2015-05-01

O F Gordeeva

{"title":"[Expression of Cancer-Testis Antigens of Magea and Mageb Families in Mouse Embryonic Fibroblasts Cultured in vitro].","authors":"O F Gordeeva","doi":"","DOIUrl":"","url":null,"abstract":"Cancer-testis antigens are expressed in the spermatogenic and cancer cells as well as in human and mouse pluripotent stem cells. However, the role of cancer-testis antigens of Mage families in the regulation of cellular processes in embryonic cells is largely unknown. In the present study comparative quantitative analysis of the gene expression of Magea and Mageb families was performed in mouse embryonic somatic cells (mouse embryonic fibroblasts, MEFs), long-term cultured in vitro and exposed to factors that inhibit and stimulate proliferation. The analysis revealed low expression of cancer-testis antigens of Mage families and showed that a lower proliferative activity of MEF at late passages was accompanied by slight up-regulation of the Magea gene expression and down-regutation of Mageb gene expression. However, modulation of the activity of MEK/ERK-signaling pathway and DNA demethylation by 5-azacytidine had no significant effects on the expression of Magea and Mageb genes in M EFs. The most essential changes in the expression levels of Mageb and Magea genes were found only when MEFs were exposed to mitomycin C. In all experimental variants, predominantly cytoplasmic localization of Mage antigens was found in MEFs at the phase of DNA synthesis, as well as at other cell cycle phases. Presumably, in actively proliferating mouse embryonic somatic cells the antigens of Magea and Mageb families can act as co-activators in the regulation of cell proliferation and other cellular functions.","PeriodicalId":19673,"journal":{"name":"Ontogenez","volume":"46 3","pages":"186-97"},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34032673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0