Oral microbiology and immunology最新文献_第5页

Does the frequency of Prevotella intermedia increase during pregnancy? 妊娠期间中普雷沃氏菌的感染频率会增加吗?

Oral microbiology and immunology Pub Date : 2009-08-01 DOI: 10.1111/j.1399-302X.2009.00509.x

M Gürsoy, G Haraldsson, M Hyvönen, T Sorsa, R Pajukanta, E Könönen

{"title":"Does the frequency of Prevotella intermedia increase during pregnancy?","authors":"M Gürsoy, G Haraldsson, M Hyvönen, T Sorsa, R Pajukanta, E Könönen","doi":"10.1111/j.1399-302X.2009.00509.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2009.00509.x","url":null,"abstract":"Introduction: The former Bacteroides intermedius, currently including Prevotella intermedia and Prevotella nigrescens, has been associated with hormone-induced pregnancy gingivitis. The aim of the present longitudinal study was to determine whether only P. intermedia or P. nigrescens, or both species, are involved in the demonstrated microbial shift during pregnancy.Methods: Subgingival plaque and saliva samples, collected from 30 healthy pregnant women and 24 healthy non-pregnant women as their controls, were examined for the presence of pigmented gram-negative anaerobes. Altogether 2628 isolates were preliminarily identified as P. intermedia sensu lato, based on phenotypic testing. Their further identification was performed by using a 16S ribosomal DNA-based polymerase chain reaction (PCR).Results: A mean of 8.3 P. intermedia sensu lato isolates from each subject/sampling was examined. During the second trimester, the mean number of P. intermedia sensu lato in plaque increased along with increasing signs of pregnancy gingivitis, and then both decreased. After delivery, gingival inflammation still decreased while the number of P. intermedia sensu lato transiently increased both in plaque and saliva. In the present study, the vast majority of isolates (95.3%) proved to be P. nigrescens and 2.5% were P. intermedia. The remaining 2.2% of the isolates could not be identified with PCR as P. intermedia or P. nigrescens. The corresponding percentages in the control population were 94.2%, 5.5%, and 0.3%.Conclusion: In the oral cavity of relatively young women without periodontitis, P. nigrescens, unlike P. intermedia, is a frequent finding. Conceivably, pregnant women harbor increasing numbers of P. nigrescens associated with pregnancy gingivitis.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 4","pages":"299-303"},"PeriodicalIF":0.0,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2009.00509.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28282344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 66

Role of Aggregatibacter actinomycetemcomitans in glutathione catabolism. 聚集菌放线菌在谷胱甘肽分解代谢中的作用。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00501.x

L Chu, X Xu, J Su, L Song, Y Lai, Z Dong, D Cappelli

{"title":"Role of Aggregatibacter actinomycetemcomitans in glutathione catabolism.","authors":"L Chu, X Xu, J Su, L Song, Y Lai, Z Dong, D Cappelli","doi":"10.1111/j.1399-302X.2008.00501.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00501.x","url":null,"abstract":"Introduction: Our previous studies demonstrated that three enzymes, gamma-glutamyltransferase (GGT), cysteinylglycinase (CGase) and cystalysin, are required for the catabolism of glutathione to produce hydrogen sulfide (H(2)S) in Treponema denticola. In this study, we examined glutathione catabolism in Aggregatibacter actinomycetemcomitans.Methods: The GGT and CGase of A. actinomycetemcomitans were determined by biological methods and GGT was characterized using a molecular biological approach.Results: A. actinomycetemcomitans showed GGT and CGase activity, but could not produce H(2)S from glutathione. The addition of recombinant T. denticola cystalysin, an l-cysteine desulfhydrase, to whole cells of A. actinomycetemcomitans resulted in the production of H(2)S from glutathione. Subsequently, we cloned A. actinomycetemcomitans GGT gene (ggt) and overexpressed the 63 kDa GGT protein. The recombinant A. actinomycetemcomitans GGT was purified and identified. The K(cat)/K(m) of the recombinant GGT from N-gamma-l-glutamyl-4-nitroaniline as substrate was 31/microm/min. The activity of GGT was optimum at pH 6.9-7.1 and enhanced by thiol-containing compounds.Conclusion: The results demonstrated that A. actinomycetemcomitans had GGT and CGase activities and that the GGT was characterized. The possible role of A. actinomycetemcomitans in glutathione metabolism and H(2)S production from oral bacteria was discussed.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"236-42"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00501.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28225415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Chronic ingestion of Porphyromonas gingivalis induces systemic nitric oxide response in mice. 慢性摄取牙龈卟啉单胞菌诱导小鼠全身一氧化氮反应。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00496.x

A Nemec, Z Pavlica, D A Crossley, M Sentjurc, A Jerin, D Erzen, M Vrecl, G Majdic, I Zdovc, M Petelin, U Skaleric

{"title":"Chronic ingestion of Porphyromonas gingivalis induces systemic nitric oxide response in mice.","authors":"A Nemec, Z Pavlica, D A Crossley, M Sentjurc, A Jerin, D Erzen, M Vrecl, G Majdic, I Zdovc, M Petelin, U Skaleric","doi":"10.1111/j.1399-302X.2008.00496.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00496.x","url":null,"abstract":"Introduction: Porphyromonas gingivalis induces nitric oxide (NO) production in various cells, systemic NO elevation being expected in chronic oral challenge.Methods: Groups of BALB/c mice were inoculated orally with either live P. gingivalis ATCC 33277 or sterile broth on days 0, 2 and 4, with or without later administration of the inducible nitric oxide synthase (iNOS) inhibitor 1400W. Plasma and tissues were harvested on day 42 for assays of tumor necrosis factor-alpha (TNF-alpha), nitrite and nitrate (NOx) and tissue NO, or histology and iNOS immunohistochemistry.Results: No signs of gingivitis were observed, but plasma NOx was significantly elevated (P = 0.028) as was TNF-alpha (P = 0.079) in P. gingivalis-inoculated animals compared with controls, NOx being reduced when 1400W was used. NO production in organs showed a similar trend, with significant elevation in liver (P = 0.017) and kidneys (P = 0.027), whereas concomitant treatment of inoculated animals with 1400W caused significant reductions in NO in aorta (P = 0.008) and kidneys (P = 0.046). Sham-inoculated 1400W-treated animals had significantly increased plasma NOx (P = 0.004) and liver NO (P = 0.04). NOx in plasma correlated significantly with NO production in lungs (0.35, P = 0.032) and kidneys (0.47, P = 0.003). Immunohistochemistry demonstrated iNOS activity in many tissues in all groups.Conclusion: Repeated oral administration of P. gingivalis induced systemic NO and NOx production in mice, probably by activating iNOS as suggested by the response to 1400W.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"204-10"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00496.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28225410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Genes responsible for dextran-dependent aggregation of Streptococcus sobrinus strain 6715. sobrinus链球菌6715菌株葡聚糖依赖性聚集的基因。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00499.x

Y Sato, K Okamoto-Shibayama, K Takada, T Igarashi, M Hirasawa

{"title":"Genes responsible for dextran-dependent aggregation of Streptococcus sobrinus strain 6715.","authors":"Y Sato, K Okamoto-Shibayama, K Takada, T Igarashi, M Hirasawa","doi":"10.1111/j.1399-302X.2008.00499.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00499.x","url":null,"abstract":"Introduction: Streptococcus sobrinus exhibits more significant dextran-dependent aggregation mediated by glucan-binding proteins than Streptococcus mutans. We have identified four glucan-binding protein C gene (gbpC) homologues designated as gbpC1, gbpC2, dblA and dblB in S. sobrinus in contrast to the single gene gbpC in S. mutans. We attempted to determine which gene is most responsible for the dextran-dependent aggregation of S. sobrinus.Methods: We introduced mutation with a chemical mutagen, 1-methyl-3-nitro-1-nitrosoguanidine, into S. sobrinus strain 6715 and analysed the four gbpC homologous gene sequences in the parental strain 6715 and an obtained aggregation-negative mutant NUM-Ssg99. We also examined the localization of proteins encoded by these genes in the mutant NUM-Ssg99.Results: The nucleotide sequences of the gbpC1, gbpC2 and dblA genes in NUM-Ssg99 were 100% identical to the homologous genes in parental strain 6715. In contrast, a truncated mutation was detected in the dblB gene and the mutant protein devoid of the LPXTG motif was confirmed by Western blot analysis to be released into the extracellular milieu.Conclusion: We conclude that the dblB gene among the four GbpC homologous protein genes is most responsible for aggregation in strain 6715.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"224-30"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00499.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28225413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Molecular and biological characterization of gtf regulation-associated genes in Streptococcus mutans. 变形链球菌gtf调控相关基因的分子生物学特性研究。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00497.x

Y Terao, R Isoda, J Murakami, S Hamada, S Kawabata

{"title":"Molecular and biological characterization of gtf regulation-associated genes in Streptococcus mutans.","authors":"Y Terao, R Isoda, J Murakami, S Hamada, S Kawabata","doi":"10.1111/j.1399-302X.2008.00497.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00497.x","url":null,"abstract":"Introduction: Surface protein antigen (PAc) and glucosyltransferases (GTF) are major adhesive molecules of Streptococcus mutans, though the mechanism of their regulation has not been fully elucidated.Methods: To investigate the regulation mechanism, we determined a nucleotide sequence in the upstream region of the pac locus in S. mutans and identified two open reading frames (ORF), designated as orf1 and orf2. Each ORF was inactivated and functional analyses were performed.Results: Western blot analyses revealed that the expression level of PAc was unaffected, while that of cell-associated GTF was diminished in both mutant strains. Furthermore, they showed higher hydrophobicity levels and an impaired sucrose-dependent adherence to smooth surfaces. RNA dot blot analysis demonstrated that transcriptions of the gtfB and the gtfC genes, which encode GTF-I and GTF-SI, respectively, were downregulated, while that of pac was comparable to the wild-type strain. In addition, the GTF activities of the mutant strains were significantly lower than those of the wild-type, though a greater amount of total glucan produced by the mutants was noted in culture supernatants.Conclusion: These findings suggest that orf1 and orf2 are associated with positive regulation of the gtfB and gtfC genes.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"211-7"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00497.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28225411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

In vitro evaluation of yoghurt starter lactobacilli and Lactobacillus rhamnosus GG adhesion to saliva-coated surfaces. 体外评价酸奶发酵剂乳酸菌和鼠李糖乳杆菌GG在唾液包被表面的粘附性。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00498.x

I Stamatova, K Kari, S Vladimirov, J H Meurman

{"title":"In vitro evaluation of yoghurt starter lactobacilli and Lactobacillus rhamnosus GG adhesion to saliva-coated surfaces.","authors":"I Stamatova, K Kari, S Vladimirov, J H Meurman","doi":"10.1111/j.1399-302X.2008.00498.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00498.x","url":null,"abstract":"Aim: The aim of the study was to evaluate the adhesion of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus rhamnosus strain GG to saliva-coated surfaces in vitro.Methods: Fifteen radiolabeled dairy L. delbrueckii subsp. bulgaricus strains and L. rhamnosus GG were tested for their ability to adhere to saliva-coated hydroxyapatite beads and polystyrene microtiter plates and the radioactivity was measured by liquid scintillation counter. The effects of lysozyme on the adhesion of lactobacilli and of pretreatment with lactobacilli on the adhesion of Streptococcus sanguinis were also assessed.Results: All strains tested adhered to saliva-coated surfaces but with significantly different binding frequencies. The adhesion of the L. delbrueckii subsp. bulgaricus strains remained lower in comparison to L. rhamnosus strain GG. One L. delbrueckii subsp. bulgaricus strain showed binding frequency comparable to S. sanguinis. Lysozyme pretreatment of the samples significantly increased lactobacillus adhesion to saliva-coated surfaces.Conclusion: The present results showed significant variations in the adhesion capacity of the Lactobacillus strains studied. Adhesion to oral surfaces is of primary importance for bacterial colonization in the mouth. Only one of the L. delbrueckii subsp. bulgaricus dairy starter culture strains investigated had a high adhesion percentage. This strain might then be considered for further investigations in the oral environment.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"218-23"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00498.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28225412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Comparative whole-genome analysis of Streptococcus mutans isolates within and among individuals of different caries status. 不同龋病个体内及个体间变形链球菌分离株的全基因组比较分析。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00495.x

L Zhang, B Foxman, D R Drake, U Srinivasan, J Henderson, B Olson, C F Marrs, J J Warren, M L Marazita

{"title":"Comparative whole-genome analysis of Streptococcus mutans isolates within and among individuals of different caries status.","authors":"L Zhang, B Foxman, D R Drake, U Srinivasan, J Henderson, B Olson, C F Marrs, J J Warren, M L Marazita","doi":"10.1111/j.1399-302X.2008.00495.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00495.x","url":null,"abstract":"Introduction: Genotypic analyses of Streptococcus mutans using fingerprinting methods depend on a few genetic loci being different but do not reveal the underlying genome-wide differences between strains.Methods: We used comparative genomic hybridization (CGH) with 70-mer oligonucleotide microarrays containing open reading frames (ORFs) from S. mutans strain UA159 to examine the genetic diversity of 44 isolates from nine children selected from a local study population in Eastern Iowa.Results: Unique strains (clones) within each child initially identified by arbitrary-priming polymerase chain reaction were confirmed by CGH. There was a wide range of variation in the hybridization patterns of the 1948 ORFs among the test isolates examined. Between 87 and 237 ORFs failed to give a positive signal among individual isolates. A total of 323 of the UA159 ORFs were absent from one or more of the test strains. These 323 variable genes seemed to be distributed across the entire UA159 genome and across all the predicted functional categories.Conclusion: This set of very close geographically and temporally collected S. mutans isolates had a degree of gene content variation as high as a previously examined global set of strains. Comparing the frequency of these variable genes, the majority of which have unknown function, among strains of different origins (i.e. different caries status) could help to determine their relevance in S. mutans cariogenicity.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"197-203"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00495.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28152389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Efficiency of oral fluid collection devices in extracting antibodies. 口服液采集装置提取抗体的效率。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00500.x

C K Chang, M E Cohen, D R Bienek

{"title":"Efficiency of oral fluid collection devices in extracting antibodies.","authors":"C K Chang, M E Cohen, D R Bienek","doi":"10.1111/j.1399-302X.2008.00500.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00500.x","url":null,"abstract":"Introduction: To facilitate diagnoses, this study determined the efficacy of commercial oral fluid collection devices for their ability to recover three human immunoglobulin isotypes; immunoglobulin A (IgA), IgG, and IgM.Methods: The sandwich enzyme-linked immunosorbent assay was used to determine antibody recovery from the following devices: (i) OraSure oral specimen collection device, (ii) saliva*sampler, (iii) ORALscreen collector, (iv) Dri-Angle, (v) no. 2 cotton roll, (vi) all-gauze sponges device, and (vii) DentaSwabs. For each isotype tested, the recovered eluate was compared with the concentration applied to the device. The performance of each device was determined at various antibody concentrations.Results: Recovery of IgA from the saliva*sampler, ORALscreen collector, Dri-Angle and cotton roll was comparable to that seeded onto the device. When compared with the seeded IgG concentration, the mean concentration of antibody recovered by each product differed by approximately +/- 9 ng/ml. The average amount of IgM recovered by the cotton roll and all-gauze sponges device was approximately 29 and 39 ng/ml, respectively, less (P < 0.0001) than that seeded on the device. For all isotypes tested, the amount of antibody recovered from the device was dependent on the initial seeding concentration.Conclusion: Collectively, these data suggest that the product used for specimen collection can affect retrieval of antibodies and potentially confound patient diagnosis.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"231-5"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00500.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28225414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Prevalence, intensity and identity of bacteraemia following conservative dental procedures in children. 儿童保守牙科手术后菌血症的患病率、强度和特征。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00492.x

H Sonbol, D Spratt, G J Roberts, V S Lucas

{"title":"Prevalence, intensity and identity of bacteraemia following conservative dental procedures in children.","authors":"H Sonbol, D Spratt, G J Roberts, V S Lucas","doi":"10.1111/j.1399-302X.2008.00492.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00492.x","url":null,"abstract":"Introduction: The aim of this study was to investigate the prevalence, intensity and microbial identity of bacteraemia following conservative dental procedures. The procedures were placement of rubber dam, use of the fast drill, use of the slow drill and placement of a matrix band and wedge.Method: Two hundred and five children and adolescents undergoing general anaesthesia for dental treatment at the Eastman Dental Hospital were recruited. Each subject was randomly allocated to one of the procedure groups. A baseline blood sample was taken before any dental treatment was carried out. A second blood sample was taken 30 s after a single conservative procedure. The blood samples were processed using lysis filtration. All bacterial isolates were identified using comparative 16 S ribosomal RNA gene sequencing. Oral Streptococcus spp. and coagulase-negative Staphylococcus spp. were further identified by comparative sodA gene sequencing.Results: The prevalence of bacteraemia was significantly greater following placement of rubber dam (P = 0.01) and placement of matrix band and wedge, compared with baseline. The intensity of bacteraemia was significantly greater following placement of rubber dam (P = 0.001) and placement of matrix band and wedge (P = 0.0001). The most frequently isolated bacteria were Streptococcus spp. (56%), Actinomyces spp. (15%) and coagulase-negative Staphylococcus spp. (15%).Conclusion: Conservative dental procedures are a significant cause of bacteraemia.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"177-82"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00492.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28152386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

The microbiota on different oral surfaces in healthy children. 健康儿童不同口腔表面的微生物群。

Oral microbiology and immunology Pub Date : 2009-06-01 DOI: 10.1111/j.1399-302X.2008.00493.x

W Papaioannou, S Gizani, A D Haffajee, M Quirynen, E Mamai-Homata, L Papagiannoulis

{"title":"The microbiota on different oral surfaces in healthy children.","authors":"W Papaioannou, S Gizani, A D Haffajee, M Quirynen, E Mamai-Homata, L Papagiannoulis","doi":"10.1111/j.1399-302X.2008.00493.x","DOIUrl":"https://doi.org/10.1111/j.1399-302X.2008.00493.x","url":null,"abstract":"Introduction: Knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes.Methods: Microbial samples were collected from five different oral habitats from a total of 93 children (age 3-12 years), attending the Dental School of the University of Athens, who were split into three age groups. A total of 38 microbial species were sought out by the checkerboard DNA-DNA hybridization technique.Results: All of the test species, except Parvimonas micra and Porphyromonas gingivalis, differed significantly among sample locations providing quite distinct microbial profiles for the different oral surfaces. Supragingival and subgingival plaque had similar profiles and exhibited higher proportions of Actinomyces species and Green complex while soft tissue samples were dominated by streptococci of the Yellow complex. The profiles of the tongue dorsum and saliva were also similar. Many of the species were in similar proportions in all three age groups for a given location. Periodontal pathogens showed increases in proportions with increasing age. Specifically, the Red complex species (Tannerella forsythia, P. gingivalis, Treponema denticola) showed a significant increase in proportion with age (P < 0.05) in all sample locations.Conclusions: The results showed a pattern of colonization in children similar to that previously found in adults. Differences in the profile between age groups suggest a gradual maturation of the oral microbiota, with it being made up of an increasing number of Orange and Red complex species.","PeriodicalId":19630,"journal":{"name":"Oral microbiology and immunology","volume":"24 3","pages":"183-9"},"PeriodicalIF":0.0,"publicationDate":"2009-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1399-302X.2008.00493.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28152387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 133