Nucleic Acids Research最新文献

Teamwork of clustered low-affinity κB sites and accessory factors regulates transcriptional strength of NF-κB RelA dimers 聚集性低亲和力κB位点和辅助因子的协同作用调节NF-κB RelA二聚体的转录强度

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-09 DOI: 10.1093/nar/gkaf846

Shandy Shahabi, Tapan Biswas, Yuting Shen, Rose Sanahmadi, Yaya Zou, Gourisankar Ghosh

{"title":"Teamwork of clustered low-affinity κB sites and accessory factors regulates transcriptional strength of NF-κB RelA dimers","authors":"Shandy Shahabi, Tapan Biswas, Yuting Shen, Rose Sanahmadi, Yaya Zou, Gourisankar Ghosh","doi":"10.1093/nar/gkaf846","DOIUrl":"https://doi.org/10.1093/nar/gkaf846","url":null,"abstract":"Non-consensus binding sites of transcription factors (TFs) are often observed within the regulatory elements of genes; however, their effect on transcriptional strength is unclear. Within the promoters and enhancers of NF-κB-responsive genes, we identified clusters of non-consensus κB DNA sites, many exhibiting low affinity for NF-κB in vitro. Deletion of these sites demonstrated their collective critical role in transcription. We explored how these “weak” κB sites exert their influence, especially given the typically low nuclear concentrations of NF-κB. Using proteomics approaches, we identified additional nuclear factors, including other DNA-binding TFs, that could interact with κB site-bound NF-κB RelA. ChIP-seq and RNA-seq analyses suggest that these accessory TFs, referred to as the TF-cofactors of NF-κB, facilitate dynamic recruitment of NF-κB to the clustered weak κB sites. Overall, the occupancy of NF-κB at promoters and enhancers appears to be defined by a collective contribution from all κB sites, both weak and strong, in association with specific cofactors. This congregation of multiple factors within dynamic transcriptional complexes is likely a common feature of transcriptional programs.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"37 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canonical translation factors eIF1A and eIF5B modulate the initiation step of repeat-associated non-AUG translation 典型翻译因子eIF1A和eIF5B调节重复相关非aug翻译的起始步骤

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-09 DOI: 10.1093/nar/gkaf994

Hayato Ito, Kodai Machida, Yuzo Fujino, Mayuka Hasumi, Soyoka Sakamoto, Yoshitaka Nagai, Hiroaki Imataka, Hideki Taguchi

{"title":"Canonical translation factors eIF1A and eIF5B modulate the initiation step of repeat-associated non-AUG translation","authors":"Hayato Ito, Kodai Machida, Yuzo Fujino, Mayuka Hasumi, Soyoka Sakamoto, Yoshitaka Nagai, Hiroaki Imataka, Hideki Taguchi","doi":"10.1093/nar/gkaf994","DOIUrl":"https://doi.org/10.1093/nar/gkaf994","url":null,"abstract":"Nucleotide repeat expansions, such as the GGGGCC repeats in C9orf72, associated with C9-ALS, are linked to neurodegenerative diseases. These repeat sequences undergo a noncanonical translation known as repeat-associated non-AUG (RAN) translation. Unlike canonical translation, RAN translation initiates from non-AUG codons and occurs in all reading frames. To identify potential regulators of RAN translation, we employed a bottom-up approach using a human factor-based reconstituted cell-free translation system to recapitulate RAN translation. This approach revealed that omission of either eIF1A or eIF5B enhanced the translation in all reading frames of C9orf72-mediated RAN translation (C9-RAN), suggesting that eIF1A and eIF5B act as repressors of RAN translation. eIF1A and eIF5B are known to contribute to the fidelity of translation initiation. In HEK293T cells, double knockdown of eIF1A and eIF5B further promoted C9-RAN compared to single knockdowns, indicating that these factors regulate C9-RAN through distinct initiation steps. Furthermore, under eIF1A knockdown conditions, the enhancement of RAN translation via the integrated stress response (ISR) was not observed in HEK293T cells, indicating that eIF1A is involved in the ISR-mediated non-AUG translation.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"83 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR-Cas12a REC2–Nuc interactions drive target-strand cleavage and constrain trans cleavage CRISPR-Cas12a - REC2-Nuc相互作用驱动靶链切割并约束反式切割

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-09 DOI: 10.1093/nar/gkaf988

Anthony Newman, Aakash Saha, Lora Starrs, Pablo R Arantes, Giulia Palermo, Gaetan Burgio

{"title":"CRISPR-Cas12a REC2–Nuc interactions drive target-strand cleavage and constrain trans cleavage","authors":"Anthony Newman, Aakash Saha, Lora Starrs, Pablo R Arantes, Giulia Palermo, Gaetan Burgio","doi":"10.1093/nar/gkaf988","DOIUrl":"https://doi.org/10.1093/nar/gkaf988","url":null,"abstract":"CRISPR-Cas12a mediates RNA-guided cleavage of double-stranded DNA in cis, after which it remains catalytically active and non-specifically cleaves single-stranded DNA in trans. Native host defence by Cas12a employs cis cleavage, which can be repurposed for the genome editing of other organisms, and trans cleavage can be used for in vitro DNA detection. Cas12a orthologues have high structural similarity and a conserved mechanism of DNA cleavage, yet highly different efficacies when applied for genome editing or DNA detection. By comparing three well-characterized Cas12a orthologues (FnCas12a, LbCas12a, and AsCas12a), we sought to determine what drives their different cis and trans cleavage and how this relates to their applied function. We integrated in vitro DNA cleavage kinetics with molecular dynamics simulations, plasmid interference in Escherichia coli, and genome editing in human cell lines. We report large differences in cis cleavage kinetics between orthologues, which may be driven by dynamic REC2-Nuc interactions. We generated and tested REC2 and Nuc mutants, including a hitherto unstudied ‘Nuc-loop’, integrity of which is critical for the function of Cas12. In total, our in vitro, in vivo, and in silico survey of Cas12a orthologues highlights key properties that drive their function in biotechnology applications.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"60 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KAT5-mediated acetylation of PC4 facilitates DNA repair by promoting chromatin reorganization kat5介导的PC4乙酰化通过促进染色质重组促进DNA修复

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-09 DOI: 10.1093/nar/gkaf974

Aayushi Agrawal, Sweta Sikder, Siddharth Singh, Rakesh Kumar Sharma, Nikhil Pallaprolu, Kalyan Mitra, Sourav Mondal, Rupa Mukhopadhyay, Jayanta Sarkar, Ramalingam Peraman, V Ravichandiran, Tapas K Kundu

{"title":"KAT5-mediated acetylation of PC4 facilitates DNA repair by promoting chromatin reorganization","authors":"Aayushi Agrawal, Sweta Sikder, Siddharth Singh, Rakesh Kumar Sharma, Nikhil Pallaprolu, Kalyan Mitra, Sourav Mondal, Rupa Mukhopadhyay, Jayanta Sarkar, Ramalingam Peraman, V Ravichandiran, Tapas K Kundu","doi":"10.1093/nar/gkaf974","DOIUrl":"https://doi.org/10.1093/nar/gkaf974","url":null,"abstract":"Human positive coactivator 4 (PC4) is a highly abundant non-histone chromatin protein involved in diverse cellular processes, including transcription regulation, genome organization, autophagy, B-cell differentiation, neurogenesis, DNA repair, etc. Most PC4 is phosphorylated in cells, which interacts with core histones and the linker histone H1 to confer the compact heterochromatin state of the genome. Knocking down PC4 at both cellular and organismal levels leads to significant chromatin decondensation, altered epigenetic landscape, enhanced autophagy, and increased DNA damage susceptibility. Here, we report that besides p300, PC4 also gets acetylated by DNA repair, facilitating lysine acetyltransferase KAT5 (Tip60) at a specific lysine residue (PC4K80) when the cells are subjected to DNA damage. The vulnerability of DNA in PC4 devoid cells was substantially reduced by reintroducing wild-type PC4 to the cells but not the mutant PC4 (PC4K80R), defective in KAT5-mediated acetylation. High-resolution microscopy techniques, including transmission electron microscopy and atomic force microscopy, are employed to visualize chromatin structural changes in response to DNA damage and repair in a Tip60-mediated PC4 acetylation-dependent manner. Presumably, KAT5-mediated acetylation of PC4 at K80 residue facilitates access to the damaged DNA by altering chromatin structures at damage sites, thus promoting DNA repair. This process could be highly significant both in cancer and in aging.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"158 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-specific BLISTER modulates miRNA biogenesis by regulating MIR transcription, HYL1 phosphorylation, and nuclear transport in Arabidopsis 拟南芥植物特异性BLISTER通过调节MIR转录、HYL1磷酸化和核转运来调节miRNA的生物发生

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-09 DOI: 10.1093/nar/gkaf1012

Shu Wang, Xin Xin, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang

{"title":"Plant-specific BLISTER modulates miRNA biogenesis by regulating MIR transcription, HYL1 phosphorylation, and nuclear transport in Arabidopsis","authors":"Shu Wang, Xin Xin, Jiedao Zhang, Xiang Li, Wei Yang, Shuxin Zhang","doi":"10.1093/nar/gkaf1012","DOIUrl":"https://doi.org/10.1093/nar/gkaf1012","url":null,"abstract":"MicroRNAs (miRNAs), processed from primary transcripts by the microprocessor complex (MC), serve as crucial post-transcriptional regulators in eukaryotes. The stability and nuclear localization of HYL1, a core MC component, are essential for maintaining complex activity. In this study, we demonstrate that the plant-specific protein BLISTER (BLI) plays a key role in miRNA biogenesis by regulating MIR transcription, HYL1 phosphorylation, and HYL1 transport in Arabidopsis. The bli mutant exhibits increased accumulation of specific miRNAs accompanied by enhanced HYL1-containing D-body formation. Biochemical evidence indicates that BLI negatively regulates MIR transcription. Moreover, BLI promotes HYL1 dephosphorylation, which facilitates its degradation. Furthermore, BLI interacts with KETCH1 to orchestrate HYL1 nuclear import. These findings establish a novel regulatory paradigm where a plant-specific protein integrates transcriptional control and post-translational modification to coordinate miRNA production, advancing our understanding of plant gene regulation mechanisms.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"128 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PhaSepDB 3.0: a comprehensive knowledgebase of phase separation-related proteins from AI-assisted curation. PhaSepDB 3.0：人工智能辅助培养的相分离相关蛋白质的综合知识库。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-08 DOI: 10.1093/nar/gkaf973

Kaiqiang You,Runyu Li,Ruixin Lian,Yuxuan Li,Hongzhining Yang,Yiran Zhou,Yangsheng Chen,Likun Wang,Zhaoqing Fan,Liwei Ma,Tingting Li

{"title":"PhaSepDB 3.0: a comprehensive knowledgebase of phase separation-related proteins from AI-assisted curation.","authors":"Kaiqiang You,Runyu Li,Ruixin Lian,Yuxuan Li,Hongzhining Yang,Yiran Zhou,Yangsheng Chen,Likun Wang,Zhaoqing Fan,Liwei Ma,Tingting Li","doi":"10.1093/nar/gkaf973","DOIUrl":"https://doi.org/10.1093/nar/gkaf973","url":null,"abstract":"Phase separation (PS) is a fundamental principle driving the formation of membraneless organelles (MLOs), which are critical for various cellular functions and pathological conditions. We present PhaSepDB 3.0 (https://db.phasep.pro/), a significantly updated knowledgebase of proteins related to PS. To address the challenges of curating a vast body of literature, we have implemented a novel human-AI collaborative workflow that integrates a large language model (LLM)-based agentic system with expert verification, enabling a major expansion and enrichment of the database. PhaSepDB 3.0 now contains 3,484 expert-curated entries for 1849 PS-related proteins, more than doubling the content of the previous version. The annotation framework has been restructured to capture deeper insights, including functional relevance, experimental evidence, and the intrinsic and extrinsic regulations of PS. A key new feature is the protein-wise summary page, which synthesizes data from multiple publications to provide a comprehensive overview of each protein's PS behaviour and functional relevance. With redesigned, user-friendly web interfaces, PhaSepDB 3.0 serves as a critical resource for the community, supporting researchers to explore the intricate basis of PS and its biological implications in greater detail.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"111 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SMART v10: three decades of the protein domain annotation resource. SMART v10：三十年的蛋白质结构域注释资源。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-08 DOI: 10.1093/nar/gkaf1023

Ivica Letunic,Peer Bork

引用次数: 0

MediaAssist: database to support the design and optimization of cell culture medium. MediaAssist：支持细胞培养基设计和优化的数据库。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-08 DOI: 10.1093/nar/gkaf982

Kalyani Thakar,Indrani Madhugiri,Chetan Gadgil,Mugdha Gadgil

{"title":"MediaAssist: database to support the design and optimization of cell culture medium.","authors":"Kalyani Thakar,Indrani Madhugiri,Chetan Gadgil,Mugdha Gadgil","doi":"10.1093/nar/gkaf982","DOIUrl":"https://doi.org/10.1093/nar/gkaf982","url":null,"abstract":"Animal cell culture is widely used for research in fundamental biology, drug discovery, and the manufacture of biopharmaceutical products such as recombinant proteins, vaccines, and cell therapies. The nutritional medium used to culture animal cells is a complex mixture of >50 components. Historically, it has been supplemented with sera such as fetal bovine serum, but commercial applications now widely use serum-free media. Developing or optimizing a medium formulation requires knowledge of chemical parameters such as solubility and stability and benefits from knowledge of biological parameters such as specific nutrient uptake rates and transporters of the different components. The complexity of medium design and optimization is increased manifold by the possibility of co-dependencies between components, where the effect of one component depends on the concentration of another. To our knowledge, there is no common repository of these chemical and biological parameters, including co-dependencies. The MediaAssist database collates this information to aid in designing and optimizing cell culture medium. Much of this information, such as co-dependencies, is dynamic, and we intend to keep updating the database as new information becomes available. MediaAssist is available at https://mediaassist.ncl.res.in.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"26 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCsRNAdb: a comprehensive resource of small noncoding RNAs across cancers. PCsRNAdb：癌症小非编码rna的综合资源。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-08 DOI: 10.1093/nar/gkaf992

Rende Huang,Jiang Li,Qi Cao,Lixia Wang,Zhixiong Shao,Haochun Yang,Xinlei Zhang,Chuanlai Yang,Xiangya Kong,Qiuyue Gu,Jianmin Wu,Tsan-Yu Chiu,Penghu Lian,Kui Wu,Feng Gao,Zhongxu Zhu

{"title":"PCsRNAdb: a comprehensive resource of small noncoding RNAs across cancers.","authors":"Rende Huang,Jiang Li,Qi Cao,Lixia Wang,Zhixiong Shao,Haochun Yang,Xinlei Zhang,Chuanlai Yang,Xiangya Kong,Qiuyue Gu,Jianmin Wu,Tsan-Yu Chiu,Penghu Lian,Kui Wu,Feng Gao,Zhongxu Zhu","doi":"10.1093/nar/gkaf992","DOIUrl":"https://doi.org/10.1093/nar/gkaf992","url":null,"abstract":"Small noncoding RNAs (sncRNAs) constitute a diverse class of endogenous transcripts, including miRNAs, piRNAs, tRNA-derived fragments (tDRs), rRNA-derived fragments (rRFs), and other small RNAs. They have been identified as pivotal regulatory elements in a wide array of biological processes and diseases. Among these, miRNA is the most extensively studied and confirmed as a key regulatory and diagnostic biomarker in tumorigenesis and development. However, the current understanding of sncRNAs other than miRNA remains limited, particularly in the field of oncology. To this end, we collected 190 datasets from GEO/SRA database, encompassing 11 114 samples across 19 cancer types, and built a user-friendly database, the Pan-Cancer Small Non-Coding RNA Database (PCsRNAdb, http://pcsrnadb.cloudna.cn/#/Home), which is developed to provide abundant resources of sncRNAs specifically designed to investigate the association between sncRNAs and cancers. PCsRNAdb comprehensively provides basic information such as the sequence, length, abundance, and target genes of sncRNAs, and further offers four advanced functionalities, including differential expression analysis, survival analysis, expression profiling analysis, and network regulatory analysis. We anticipate that such platform offers invaluable resources and effective analytical tools for researchers in related fields and is expected to facilitate cancer-related research in this domain.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"61 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

So3D: a comprehensive three-dimensional spatial omics resource for decoding tissue architecture in physiology and disease. So3D：一个全面的三维空间组学资源，用于解码生理和疾病中的组织结构。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-10-08 DOI: 10.1093/nar/gkaf998

Hongying Zhao,Xiangzhe Yin,Siyao Wang,Zhichao Geng,Wentong Yu,Hongzheng Yu,Shangwei Ning,Li Wang

{"title":"So3D: a comprehensive three-dimensional spatial omics resource for decoding tissue architecture in physiology and disease.","authors":"Hongying Zhao,Xiangzhe Yin,Siyao Wang,Zhichao Geng,Wentong Yu,Hongzheng Yu,Shangwei Ning,Li Wang","doi":"10.1093/nar/gkaf998","DOIUrl":"https://doi.org/10.1093/nar/gkaf998","url":null,"abstract":"Cells function within intricate three-dimensional (3D) architectures to form tissues and organs. Constructing 3D tissue structure is critical for understanding cellular states, biological processes, and intercellular interactions. Herein, we describe a comprehensive 3D spatial omics resource, So3D (http://bio-bigdata.hrbmu.edu.cn/So3D or https://so3d.bio-database.com/), which aims to construct 3D tissue architecture and dissect biological processes occurring in 3D space from multiple perspectives. We systematically collected 72 sets of 3D spatial transcriptome datasets, involving 1 132 902 spots across 882 slices from four species, and matched reference single-cell RNA-sequencing data, covering 763 893 cells from 28 datasets. So3D also provides multiple flexible analysis modules for retrieving and analyzing 3D tissue, such as inference of 3D spatial domains and gene expression patterns across 3D tissue regions, 2D spatial slices, and single-cell datasets; mapping of cell type distribution and cell-cell communication networks to explore intercellular crosstalk in 3D space; and functional annotation of biological pathways and signaling networks within 3D tissue contexts. Collectively, So3D provides comprehensive insights for investigating biologically meaningful 3D spatial domains, mapping local gene expression landscapes, functional state, and communication networks in tissue, and may also serve as an efficient and reliable tool for understanding the tissue microenvironment and discovering biomarkers.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"108 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0