Nucleic Acids Research最新文献

APD6: the antimicrobial peptide database is expanded to promote research and development by deploying an unprecedented information pipeline. APD6：扩展抗菌肽数据库，通过部署前所未有的信息管道来促进研究和开发。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-10 DOI: 10.1093/nar/gkaf860

Guangshun Wang,Cindy Schmidt,Xia Li,Zhe Wang

{"title":"APD6: the antimicrobial peptide database is expanded to promote research and development by deploying an unprecedented information pipeline.","authors":"Guangshun Wang,Cindy Schmidt,Xia Li,Zhe Wang","doi":"10.1093/nar/gkaf860","DOIUrl":"https://doi.org/10.1093/nar/gkaf860","url":null,"abstract":"The global antibiotic resistance issue constitutes a driving force for developing host defense antimicrobial peptides (AMPs) into a new generation of antibiotics. To facilitate this development, we report the antimicrobial peptide database version 6 (APD6) with (i) the consolidated database platform, (ii) the most comprehensive AMP information pipeline (AMPIP), and (iii) the expanded wheel of function. As of 18 March 2025, the APD6 platform housed records for 5188 peptides, including 3306 natural, 1380 synthetic, and 239 predicted AMPs with systematic classification schemes for each group. Based on the refined dataset, we present an updated view and findings on natural AMPs. Natural AMPs provide a fundamental dataset for peptide design and predicting potential AMPs. While current artificial intelligence prediction of AMPs is limited to activity and hemolysis, the APD6 provides new positive and negative datasets (e.g. pH, salt, serum effects, and resistance) for building advanced AI prediction models to identify more robust antibiotics. The AMPIP covers information ranging from peptide discovery, in vitro/in vivo activity and toxicity data, to clinical trials. In addition, the APD6 (available at https://aps.unmc.edu) contains an expanded wheel of peptide functions (e.g. anticancer and antidiabetic), allowing for developing peptide therapeutics outside the antibiotic arena.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"24 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TCRdb 2.0: an updated T-cell receptor sequence database. TCRdb 2.0：更新的t细胞受体序列数据库。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-09 DOI: 10.1093/nar/gkaf876

Tao Yue,Si-Yi Chen,Wen-Kang Shen,Yu Liao,Qian Lei,An-Yuan Guo

{"title":"TCRdb 2.0: an updated T-cell receptor sequence database.","authors":"Tao Yue,Si-Yi Chen,Wen-Kang Shen,Yu Liao,Qian Lei,An-Yuan Guo","doi":"10.1093/nar/gkaf876","DOIUrl":"https://doi.org/10.1093/nar/gkaf876","url":null,"abstract":"T-cell receptor (TCR) repertoire sequencing allows researchers to analyze millions of TCRs, providing unparalleled precision in understanding immune responses and enabling broad applications. However, existing TCR-related databases are based on a limited number of samples. Here, we present TCRdb2.0 (https://guolab.wchscu.cn/TCRdb2/#/), an updated and significantly expanded resource with enriched data and enhanced functionalities. TCRdb2.0 incorporates ∼700 million TCR sequences derived from 19 701 TCR-Seq samples across 46 tissues and 147 clinical conditions, making it the most comprehensive TCR sequence database to date. The homepage of TCRdb2.0 has powerful browsing, searching and downloading functions. It displays multiple features of TCR in sample and project levels. Compared to the previous release, TCRdb2.0 has the following major improvements: (i) a substantial increase of TCR sequences, from 277 million to ∼700 million; (ii) inclusion of TCR sequences from Gamma delta (γδ) T cells; (iii) integration of therapy-related TCR-Seq datasets, such as programmed cell death 1 (PD-1) blockade immunotherapy; (iv) construction of the largest TCR sequence reference from healthy samples; and (v) redesign of a new search and download function, enabling flexible queries and downloads. With its extensive data and user-friendly web interface, TCRdb2.0 will serve as an invaluable resource for functional studies of TCRs in both health and disease.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"17 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12a DTR system: a topology-guided Cas12a assay for specific dual detection of RNA and DNA targets. CRISPR/Cas12a DTR系统：一种拓扑引导的Cas12a检测，用于特异性双重检测RNA和DNA靶标。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf893

Qingyuan Jiang,Shuqi Jin,Zhichao Qin,Junqi Zhang,Ruyi He,Zhuo Chen,Bin Qiao,Jie Qiao,Yi Liu

{"title":"CRISPR/Cas12a DTR system: a topology-guided Cas12a assay for specific dual detection of RNA and DNA targets.","authors":"Qingyuan Jiang,Shuqi Jin,Zhichao Qin,Junqi Zhang,Ruyi He,Zhuo Chen,Bin Qiao,Jie Qiao,Yi Liu","doi":"10.1093/nar/gkaf893","DOIUrl":"https://doi.org/10.1093/nar/gkaf893","url":null,"abstract":"The CRISPR/Cas12a technology has revolutionized molecular diagnostics. However, existing Cas12a systems depend on continuous target DNA activation, which limits them to single-target detection. In this study, we developed a novel topology-guided Cas12a system, the double-target responsive (DTR) system, capable of being activated by noncontiguous dual RNA/DNA targets. The DTR system employs two split CRISPR RNA (crRNA) fragments and two Cas12a proteins that cooperatively reconstitute upon recognizing two nucleic acid activators. We demonstrated the DTR system's ability to specifically detect dual nucleic acid substrates in a single readout, achieving a detection limit of 78 fM for RNA and exceptional specificity for single-nucleotide variations. Additionally, we successfully applied the DTR system to clinical samples, enabling simultaneous detection of two oral squamous cell carcinoma-related microRNAs (miR-155 and miR-let-7a), thereby distinguishing healthy individuals from patients. This work establishes an efficient Cas12a-based platform for sensitive, simultaneous, and discriminative detection of RNA and DNA targets, enhancing the versatility of Cas12a in analytical detection and clinical diagnosis.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"38 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structures of distinct parallel and antiparallel DNA G-quadruplexes reveal structural polymorphism in C9orf72 G4C2 repeats. 不同的平行和反平行DNA g -四联体的晶体结构揭示了C9orf72 G4C2重复序列的结构多态性。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf879

Yanyan Geng,Changdong Liu,Haitao Miao,Monica Ching Suen,Yuanyuan Xie,Bingchang Zhang,Wanhong Han,Caiming Wu,Haixia Ren,Xueqin Chen,Hwan-Ching Tai,Zhanxiang Wang,Guang Zhu,Qixu Cai

{"title":"Crystal structures of distinct parallel and antiparallel DNA G-quadruplexes reveal structural polymorphism in C9orf72 G4C2 repeats.","authors":"Yanyan Geng,Changdong Liu,Haitao Miao,Monica Ching Suen,Yuanyuan Xie,Bingchang Zhang,Wanhong Han,Caiming Wu,Haixia Ren,Xueqin Chen,Hwan-Ching Tai,Zhanxiang Wang,Guang Zhu,Qixu Cai","doi":"10.1093/nar/gkaf879","DOIUrl":"https://doi.org/10.1093/nar/gkaf879","url":null,"abstract":"The abnormal expansion of GGGGCC (G4C2) repeats in the noncoding region of the C9orf72 gene is a major genetic cause of two devastating neurodegenerative disorders, amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). These G4C2 repeats are known to form G-quadruplex (G4) structures, which are hypothesized to contribute to disease pathogenesis. Here, we demonstrated that four DNA G4C2 repeats can fold into two structurally distinct G4 conformations: a parallel and an antiparallel topology. The high-resolution crystal structure of the parallel G4 reveals an eight-layered dimeric assembly, formed by two identical monomeric units. Each unit contains four stacked G-tetrads connected by three propeller CC loops and is stabilized through 5'-to-5' π-π interactions and coordination with a central K+ ion. Notably, the 3'-ending cytosines form a C·C+·C·C+ quadruple base pair stacking onto the adjacent G-tetrad layer. In contrast, the antiparallel G4 adopts a four-layered monomeric structure with three edgewise loops, where the C6 and C18 bases engage in stacking interaction with neighboring G-tetrad via a K+ ion. These structurally distinct G-quadruplexes provide mechanistic insights into C9orf72-associated neurodegeneration and offer potential targets for the development of structure-based therapeutic strategies for ALS and FTD.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"100 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The advantage of periodic over constant signalling in microRNA-mediated regulation. 在microrna介导的调控中，周期性信号优于恒定信号。

IF 13.1 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf867

Elsi Ferro, Candela L Szischik, Alejandra C Ventura, Carla Bosia

{"title":"The advantage of periodic over constant signalling in microRNA-mediated regulation.","authors":"Elsi Ferro, Candela L Szischik, Alejandra C Ventura, Carla Bosia","doi":"10.1093/nar/gkaf867","DOIUrl":"10.1093/nar/gkaf867","url":null,"abstract":"Cells may exploit oscillatory gene expression to encode biological information. Temporal features of oscillations, such as pulse frequency and amplitude, are determinant for the outcome of signalling pathways. However, little effort has been devoted to unveiling the role of pulsatility in the context of post-transcriptional gene regulation, where microRNAs act by binding to RNAs and regulate their expression. Here, we study the effects of periodic against constant microRNA synthesis within minimal microRNA-target networks. We find that there is a repressive advantage of pulsatile over constant microRNA synthesis, and that the extent of repression depends on the frequency of pulses, thus uncovering frequency preference behaviours. We show that the preference for specific input frequencies is determined by relative microRNA and target kinetic rates and can lead to exclusive frequency-dependent repression on distinct RNA species, thereby highlighting a potential mechanism of selective dynamical target regulation. Moreover, we show that frequencies observed in periodically expressed microRNAs, such as those involved in circadian rhythms and development, can be selectively favored. Our findings might have implications for experimental studies aimed at understanding how periodic patterns drive biological responses through microRNA-mediated signalling and provide suggestions for validation in synthetic networks.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"53 17","pages":""},"PeriodicalIF":13.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12418391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA G-quadruplexes emerge from a compacted coil-like ensemble via multiple pathways. RNA g -四联体通过多种途径从紧凑的线圈状集合中出现。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf872

Pavlína Pokorná,Vojtěch Mlýnský,Jiří Šponer,Petr Stadlbauer

{"title":"RNA G-quadruplexes emerge from a compacted coil-like ensemble via multiple pathways.","authors":"Pavlína Pokorná,Vojtěch Mlýnský,Jiří Šponer,Petr Stadlbauer","doi":"10.1093/nar/gkaf872","DOIUrl":"https://doi.org/10.1093/nar/gkaf872","url":null,"abstract":"RNA G-quadruplexes (rG4s) are emerging as vital structural elements involved in processes like gene regulation, translation, and genome stability. Found in untranslated regions of messenger RNAs (mRNAs), they influence translation efficiency and mRNA localization. Additionally, rG4s of long noncoding RNAs and telomeric RNA play roles in RNA processing and cellular aging. Despite their significance, the atomic-level folding mechanisms of rG4s remain poorly understood due to their complexity. We studied the folding of the r(GGGA)3GGG and r(GGGUUA)3GGG (TERRA) sequences into parallel-stranded rG4 using all-atom enhanced-sampling molecular dynamics simulations, applying well-tempered metadynamics coupled with solute tempering. The obtained folding pathways suggest that RNA initially adopts a compacted coil-like ensemble characterized by dynamic guanine stacking and pairing. The three-quartet rG4 gradually forms from this compacted coil ensemble via diverse routes involving strand rearrangements and guanine incorporations. While the folding mechanism is multipathway, various two-quartet rG4 structures appear to be a common transitory ensemble along most routes. Thus, the process seems more complex than previously predicted, as G-hairpins or G-triplexes do not act as distinct intermediates, even though some are occasionally sampled. We also discuss the challenges of applying enhanced sampling methodologies to such a multidimensional free-energy surface and address the force-field limitations.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"32 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesizing supercoiled circular DNA molecules in vitro. 体外合成超螺旋环状DNA分子。

IF 13.1 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf889

Sepideh Rezaei, Monica Moncada-Restrepo, Sophia Leng, Jeremy W Chambers, Fenfei Leng

{"title":"Synthesizing supercoiled circular DNA molecules in vitro.","authors":"Sepideh Rezaei, Monica Moncada-Restrepo, Sophia Leng, Jeremy W Chambers, Fenfei Leng","doi":"10.1093/nar/gkaf889","DOIUrl":"10.1093/nar/gkaf889","url":null,"abstract":"Supercoiled (Sc) circular DNA, such as plasmids, are essential in molecular biology and hold strong therapeutic potential. However, they are typically produced in Escherichia coli, resulting in bacterial methylations, unnecessary sequences, and contaminants that hinder certain applications including clinical uses. These limitations could be avoided by synthesizing plasmids entirely in vitro, but synthesizing high-purity Sc circular DNA biochemically remains a significant technical challenge. To overcome this challenge, we have developed two novel biochemical methods for in vitro synthesis of Sc circular DNA. Linear DNA with two loxP sites in the same orientation is generated by polymerase chain reaction or rolling circle amplification. Cre recombinase efficiently converts the linear DNA into relaxed circular DNA. T5 exonuclease is then used to digest unwanted linear DNA, and topoisomerases are employed to generate Sc circular DNA. Using this approach, we synthesized EGFP-FL, a 2 kb mini-circular DNA encoding essential EGFP expression elements. EGFP-FL transfected HeLa and C2C12 cells with significantly higher efficiency than its E. coli-derived counterpart. These methods enable the efficient production of Sc circular DNA from 196 bp to several kb, and in quantities from micrograms to milligrams, providing a versatile, scalable, and bacteria-free platform for basic research and therapeutic applications.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"53 17","pages":""},"PeriodicalIF":13.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based analysis of the impact of 5' untranslated region on protein expression. 基于机器学习的5'非翻译区对蛋白表达影响分析。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf861

Linfeng Wang,Sujia Liu,Jia-Xin Huang,Haifeng Zhu,Shuyu Li,Yannan Li,Sen Chen,Jianying Han,Yin Zhu,Jiahao Wu,Wentao Liao,Hongmei Zhang,Haiyan Zeng,Shaoting Li,Shuping Zhao,Bingwei Wang,Jiaqi Lin,Ji Zeng

{"title":"Machine learning-based analysis of the impact of 5' untranslated region on protein expression.","authors":"Linfeng Wang,Sujia Liu,Jia-Xin Huang,Haifeng Zhu,Shuyu Li,Yannan Li,Sen Chen,Jianying Han,Yin Zhu,Jiahao Wu,Wentao Liao,Hongmei Zhang,Haiyan Zeng,Shaoting Li,Shuping Zhao,Bingwei Wang,Jiaqi Lin,Ji Zeng","doi":"10.1093/nar/gkaf861","DOIUrl":"https://doi.org/10.1093/nar/gkaf861","url":null,"abstract":"The 5' untranslated region (5'UTR) plays a crucial regulatory role in messenger RNA (mRNA), with modified 5'UTRs extensively utilized in vaccine production, gene therapy, etc. Nevertheless, manually optimizing 5'UTRs may encounter difficulties in balancing the effects of various cis-elements. Consequently, multiple 5'UTR libraries have been created, and machine learning models have been employed to analyze and predict translation efficiency (TE) and protein expression, providing insights into critical regulatory features. On the one hand, these screening libraries, based on TE and mean ribosome load, struggle to accurately quantify protein expression; on the other hand, a precise method for quantifying 5'UTRs necessitates a significantly costlier library. To resolve this dilemma, we constructed a library utilizing firefly luciferase as the reporter to measure accurate protein expression. In addition, we optimized the library construction method by clustering mRNA sequences to reduce redundant data and minimize the size of the dataset. This dual strategy by increasing accuracy and reducing dataset size was found to be effective in predicting the 5'UTRs from the PC3 cell line.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"307 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing. DDX6与DDX3X相互作用抑制microrna介导的沉默中的翻译。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf868

Yanyan Lu,Meng Tao,Hong Su,Yiren Tu,Ji-Ping Wang,Masahiko Kuroda,Xiaozhong Wang

引用次数: 0

Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA. 缺乏典型DNA结合域的苔藓BRCA2促进同源重组并与DNA结合。

IF 14.9 2区生物学

Nucleic Acids Research Pub Date : 2025-09-05 DOI: 10.1093/nar/gkaf856

Alice Chanteau,Suliane Quilleré,Arthur Crouset,Sreejith Allipra,Ulysse Tuquoi,Pierre-François Perroud,Simona Miron,Pauline Dupaigne,Sophie Zinn-Justin,Fabien Nogué,Rajeev Kumar

{"title":"Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA.","authors":"Alice Chanteau,Suliane Quilleré,Arthur Crouset,Sreejith Allipra,Ulysse Tuquoi,Pierre-François Perroud,Simona Miron,Pauline Dupaigne,Sophie Zinn-Justin,Fabien Nogué,Rajeev Kumar","doi":"10.1093/nar/gkaf856","DOIUrl":"https://doi.org/10.1093/nar/gkaf856","url":null,"abstract":"BRCA2 is crucial for mediating homology-directed DNA repair (HDR) through its binding to single-stranded DNA (ssDNA) and the recombinases RAD51 and DMC1. Most BRCA2 orthologs have a canonical DNA-binding domain (DBD) with the exception of Drosophila melanogaster. It remains unclear whether such a noncanonical BRCA2 variant without DBD possesses a DNA-binding activity. Here, we identify a new noncanonical BRCA2 in the model plant Physcomitrium patens (PpBRCA2). We establish that PpBRCA2 is essential for genome integrity maintenance, somatic DNA double-strand break (DSB) repair, HDR-mediated gene targeting, and RAD51 foci recruitment at DNA break sites. PpBRCA2 is also critical for DSB repair during meiosis. Interestingly, PpBRCA2 interacts strongly with RAD51 but weakly with DMC1, suggesting a distinct meiotic function compared to other BRCA2 homologs. Despite lacking the canonical DBD, PpBRCA2 binds ssDNA through its disordered N-terminal region and efficiently promotes HDR. Our work highlights that the ssDNA binding capacity of BRCA2 homologs is conserved regardless of the presence of a canonical DBD and provides a deeper understanding of BRCA2's functional diversity across species.","PeriodicalId":19471,"journal":{"name":"Nucleic Acids Research","volume":"130 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0