Notulae Botanicae Horti Agrobotanici Cluj-napoca最新文献_第8页

Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells 梧桐根提取物对宫颈癌HeLa细胞的抗癌活性评价

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-04 DOI: 10.15835/nbha51312990

Benjaporn BURANRAT, Pornpimon WONGSUWAN, Wichuda PHIPHITPHIBUNSUK

{"title":"Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells","authors":"Benjaporn BURANRAT, Pornpimon WONGSUWAN, Wichuda PHIPHITPHIBUNSUK","doi":"10.15835/nbha51312990","DOIUrl":"https://doi.org/10.15835/nbha51312990","url":null,"abstract":"Plumbago indica L. has a various pharmacological activity; however, the anticancer activity in cervical cancer has less information. To examine the antiproliferative, apoptotic, and antimigratory activity of Plumbago indica root extract on cervical cancer cells. The extract of P. indica was explored for cell viability, apoptosis, and migration by sulforhodamine B, colony formation, cell cycle distribution, Wound healing, Annexin V-FITC staining apoptotic, mitochondrial function, and reactive oxygen species (ROS) formation. The data indicated that P. indica extract suppressed HeLa cervical cells viability by dose- and time-dependent manner along with the reduction of cancer cell number and colony forming ability. After 48 h of incubation period, the PR extract exhibited a significant reduction of migratory ability by dose-dependent manner. Furthermore, these extracts significantly showed a reduction of cell viability along with induction of late apoptosis. The mechanism of action was shown by detecting mitochondrial dysfunction and activating of ROS formation on HeLa cells-treated with P. indica extract. The extract of P. indica has a growth inhibition, migratory suppression, and apoptotic induction. The extract has an anticancer activity by reducing mitochondrial function and activating ROS formation.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into tomato spotted wilt orthotospovirus (TSWV) infections in Türkiye: Molecular detection, phylogenetic analysis, and in silico docking study 番茄斑点枯萎正畸病毒(TSWV)在<s:1>基ye中的感染新认识:分子检测、系统发育分析和计算机对接研究

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-04 DOI: 10.15835/nbha51313245

Mustafa USTA, Abdullah GÜLLER, Serap DEMİREL, Gülüstan KORKMAZ, Zeynelabidin KURT

{"title":"New insights into tomato spotted wilt orthotospovirus (TSWV) infections in Türkiye: Molecular detection, phylogenetic analysis, and in silico docking study","authors":"Mustafa USTA, Abdullah GÜLLER, Serap DEMİREL, Gülüstan KORKMAZ, Zeynelabidin KURT","doi":"10.15835/nbha51313245","DOIUrl":"https://doi.org/10.15835/nbha51313245","url":null,"abstract":"Seventy-eight tomato and pepper plants showing symptoms were tested for tomato spotted wilt orthotospovirus (TSWV) using specific primers targeting the full coat protein gene (CP) through RT-PCR. Plant samples were collected in Denizli region, Türkiye during September 2021. The PCR screening results revealed that 23 samples were infected with TSWV, indicating an infection rate of 29.48%. PCR products were subsequently sequenced bidirectionally, and the obtained sequences were deposited in GenBank under the accession numbers ON323583-84 for pepper, and OQ597214 for tomato. Phylogenetic analysis demonstrated a close relationship between the Turkish-Denizli TSWV isolates and previously reported isolates from tomato and pepper plants in Türkiye (KM407603), Hungary (KJ649612), and Serbia (GU369723). Moreover, in this study, three commercial chemicals and five selected phytochemicals were docked against the TSWV CP to assess their binding energies. The docking results indicate that the tested phytochemicals exhibit promising performance compared to other commercial chemicals. This study represents the first molecular and phylogenetic report on TSWV isolates in pepper and tomato plants in Denizli province, Türkiye. Furthermore, the interaction between TSWV and the selected compounds through in silico docking has been reported for the first time.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135453441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ethylene modulates root growth and mineral nutrients levels in trifoliate orange through the auxin-signaling pathway 乙烯通过生长素信号通路调节三叶橙根系生长和矿质营养水平

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313269

Ceng-Hong HU, Shi-Dong YUAN, Cui-Ling TONG, De-Jian ZHANG, Ren-Hua HUANG

{"title":"Ethylene modulates root growth and mineral nutrients levels in trifoliate orange through the auxin-signaling pathway","authors":"Ceng-Hong HU, Shi-Dong YUAN, Cui-Ling TONG, De-Jian ZHANG, Ren-Hua HUANG","doi":"10.15835/nbha51313269","DOIUrl":"https://doi.org/10.15835/nbha51313269","url":null,"abstract":"Roots are fundamental to plant growth, with their development being profoundly influenced by water, mineral nutrients, and notably, plant hormones. Citrus, a leading horticultural crop cultivated globally, holds immense economic value. This study tried to investigate the roles and mechanisms effect of foliar spray 1.0 μmol/L ethephon (ETH) of ethylene and 5.0 μmol/L AgNO3 (an ethylene inhibitor) its inhibitor with auxin on root growth and mineral nutrients content levels in trifoliate orange under sand culture conditions for 45 days. Our findings indicate that ethephon treatment significantly boosted the number of lateral roots, root fresh weight, and enhanced the mineral nutrient content (N, P, K, B, Ca, Mg, Fe, Mn, and Zn, with the exception of Cu) in both root and shoot tissues. In contrast, the inhibitors notably reduced the length of lateral roots and decreased the content of P, and Fe in both root and shoot, whilst elevating Zn contents. Furthermore, ETH incited the up-regulation of auxin biosynthesis and transportation genes, leading to an increased endogenous auxin content in the lateral root-hair region. whereas, the inhibitors produced an opposite effect. We thus conclude that ethylene modulates auxin transportation and biosynthesis. This activity stimulates the auxin signaling pathway locally-specifically, in the root hair zone in the lateral root, which in turn, regulates root growth and mineral nutrient content.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between habitat factors and the nutrient contents of wild Allium victorialis L. in the Changbai Mountains 长白山野生葱生境因子与营养成分的关系

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313194

Bo FENG, Guanghua XU, Xiufeng WANG, Xuejiao SU, Jiayi LIU, Chenglong LI, Jianjun ZOU, Kaipeng ZHANG, Jun REN, Shuyao SONG

{"title":"The relationship between habitat factors and the nutrient contents of wild Allium victorialis L. in the Changbai Mountains","authors":"Bo FENG, Guanghua XU, Xiufeng WANG, Xuejiao SU, Jiayi LIU, Chenglong LI, Jianjun ZOU, Kaipeng ZHANG, Jun REN, Shuyao SONG","doi":"10.15835/nbha51313194","DOIUrl":"https://doi.org/10.15835/nbha51313194","url":null,"abstract":"Allium victorialis L. (Family: Liliaceae) is an herb with nutritional and medicinal properties. In Jilin Province, China, A. victorialis is mainly distributed in the Changbai Mountains and grows in various habitat conditions. However, the relationship between habitat factors and the nutritional quality of A. victorialis in the Changbai Mountains has not yet been examined. We assessed the nutritional quality of five A. victorialis populations growing in five different habitats in the Changbai Mountains and analyzed the relationship between nutritional quality and habitat factors. Allium victorialis populations in this region were primarily found in the undergrowth at elevations above 500 m and within specific ranges of air temperature, air relative humidity, soil temperature, and soil water content. Among the habitat factors investigated, canopy density significantly affected several nutritional components of A. victorialis; however, elevation had a significant effect only on the total flavonoid content, and the vitamin C content was not strongly associated with the main habitat factors in this study. During germplasm selection and artificial cultivation, it is important to simulate the growth conditions of the original habitat. Our results provide useful information for site selection and environmental condition optimization for the artificial cultivation of A. victorialis.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo polyamine applications to globe artichoke: Effects on plant growth-development and some bioactive components 多胺在洋蓟中的体内应用:对植物生长发育的影响及一些生物活性成分

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313285

Tugce OZSAN KILIC

{"title":"In vivo polyamine applications to globe artichoke: Effects on plant growth-development and some bioactive components","authors":"Tugce OZSAN KILIC","doi":"10.15835/nbha51313285","DOIUrl":"https://doi.org/10.15835/nbha51313285","url":null,"abstract":"The globe artichoke, Cynara cardunculus var. scolymus (L.), is an important member of the Asteraceae family, being used as a food and due to it has certain health-improving qualities in the treatment of various disorders since ancient times. Low molecular weight organic compounds with a role in several crucial cellular and physiological processes of plants are known as polyamines. They participate in processes such as cell division, differentiation, and growth, the sprouting of seeds, root formation, and flower and fruit development. They have been identified as particularly efficient against biotic and abiotic stress factors. The present research aimed that the evaluation of effects of various polyamine (PA) types and concentrations on three different artichoke cultivars by biometric measurements and biochemical analyses. For this purpose, seedlings of three artichoke cultivars (‘Sakız OP’, ‘Bayrampaşa OP’, and ‘Olympus F1’) were sprayed with various concentrations (0, 50, 100 and 250 mg L-1) of three distinct types of significant polyamines, including putrescine, spermidine, and spermine. Several growth parameters were examined in the current research. The effects on the total phenol and antioxidant capacities, as well as changes in the amounts of cynarin and 1,5-O-dicafeoylquinic acid, were also evaluated. The results revealed that there were statistically differences based on cultivars and treatment concentrations. Among the three forms of PA spermine was potent at increasing total phenolic content, while putrescine was effective at increasing total antioxidant content. On the other hand, putrescine, spermine and, spermidine had no positive effects on cynarin and 1,5-O-dicaffeoyquinic acid levels. Results clearly revealed that each biometric data and biochemical content could not be determined by a single polyamine type and concentration. It can be concluded that to improve the biometric values and biochemical content are depending on cultivar and dosage of various PAs.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Processing waste: bioactive components and antioxidant capacity of cold-pressed oils from some fruit seeds 加工废料:某些水果种子冷榨油的生物活性成分和抗氧化能力

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313241

Aleksandra ĆIRKOVIĆ, Mirjana DEMIN, Milica FOTIRIĆ AKŠIĆ, Biljana RABRENOVIĆ

{"title":"Processing waste: bioactive components and antioxidant capacity of cold-pressed oils from some fruit seeds","authors":"Aleksandra ĆIRKOVIĆ, Mirjana DEMIN, Milica FOTIRIĆ AKŠIĆ, Biljana RABRENOVIĆ","doi":"10.15835/nbha51313241","DOIUrl":"https://doi.org/10.15835/nbha51313241","url":null,"abstract":"In this study, the possible use of seeds after fruit processing to obtain unconventional cold-pressed edible oil was investigated. For this purpose, seeds of quince, sour cherries and plum were used. Fatty acid composition, antioxidant activity, peroxide and acid value, oxidative stability, and tocopherols and phytosterols content were determined in the studied oils. Plum seed oil was dominated by oleic acid, while quince and sour cherry seed oils contained abundant linoleic acid. The total polyphenol content in the studied oils ranged from 2.28 to 9.03 mg GAE 100 mL-1. Antioxidant properties (ABTS, DPPH, FRP) were associated with tocopherol content. All three studied oils were rich in β-sitosterol. The oxidative stability of the studied oil samples varied and ranged from 1.92 h-31.17 h. Quince seed oil had the highest content of α-tocopherol (44.30 mg 100 g-1) and plum seed oil had the lowest (3 mg 100 g-1), while sour cherry seed oil had the highest content of β+γ-tocopherol (17.19 mg 100 g-1). The results show that oil from quince, sour cherry, and plum seeds is suitable for the production of a high quality cold-pressed oil. The use of this type of waste from fruit processing contributes to waste reduction and promotes the circular economy.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NIRS and multivariate methods for discrimination of morning glory species at different growth stages 近红外光谱和多变量方法鉴别不同生长阶段的牵牛花

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313231

Andreísa F. BRAGA, Lívia C. De CARVALHO, Diogo P. CORREA da SILVA, Thamires G. ANTUNES, Luis CARLOS CUNHA JÚNIOR, Gustavo H. De ALMEIDA TEIXEIRA, Pedro L. da COSTA AGUIAR ALVES

{"title":"NIRS and multivariate methods for discrimination of morning glory species at different growth stages","authors":"Andreísa F. BRAGA, Lívia C. De CARVALHO, Diogo P. CORREA da SILVA, Thamires G. ANTUNES, Luis CARLOS CUNHA JÚNIOR, Gustavo H. De ALMEIDA TEIXEIRA, Pedro L. da COSTA AGUIAR ALVES","doi":"10.15835/nbha51313231","DOIUrl":"https://doi.org/10.15835/nbha51313231","url":null,"abstract":"Morning glory species are weeds very common in tropical crops, where they cause direct and indirect damage. The management of these species primarily relies on the application of herbicides, disregarding the growth stage and spatial distribution. Studies addressing new techniques for identifying these species may contribute to the development of proximal sensors for carrying out specific and rational management. Thus, the objective of this work was to use near infrared spectroscopy (NIRS) and multivariate analysis to discriminate two species of morning glory in three growth stages. NIRS spectra were collected from Ipomoea hederifolia and Merremia aegyptia were collected at three different stages in the spectral range of 4.000 to 10.000 cm-1. PCA and PC-LDA were used to analyze the entire spectrum and specific bands. NIRS associated with PCA and PC-LDA were sufficient to discriminate I. hederifolia and M. aegyptia species and their growth stages. PCA allowed a proper segregation of stages and species when applied individually PC-LDA correctly classified between 90.93 to 100% of species and stages. The best discrimination results were observed in the NIR spectra ranges from 4.500 to 6.000 cm-1 and 4.500 to 6.000 + 6.500 to 7.750 cm-1. This study represents an advance in the research and implementation of NIRS technology to discriminate weed species for the future development of equipment to assist in the adoption and/or performance of a specific management of weeds, capable of contributing to the reduction in the use of herbicides in crops.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perennial forage legumes as an element of sustainable systems 多年生牧草豆科植物作为可持续系统的一个要素

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313240

Dalibor D. TOMIĆ, Vladeta I. STEVOVIĆ, Dragan ĐUROVIĆ, Miloš MARJANOVIĆ, Milomirka R. MADIĆ, Nenad PAVLOVIĆ, Đorđe LAZAREVIĆ, Mirjana PETROVIĆ, Mirjana RADOVANOVIĆ

{"title":"Perennial forage legumes as an element of sustainable systems","authors":"Dalibor D. TOMIĆ, Vladeta I. STEVOVIĆ, Dragan ĐUROVIĆ, Miloš MARJANOVIĆ, Milomirka R. MADIĆ, Nenad PAVLOVIĆ, Đorđe LAZAREVIĆ, Mirjana PETROVIĆ, Mirjana RADOVANOVIĆ","doi":"10.15835/nbha51313240","DOIUrl":"https://doi.org/10.15835/nbha51313240","url":null,"abstract":"In the current intensive systems of agricultural production, many important features, i.e., functions of the agroecosystem have been degraded and disrupted. The intensification of agricultural production inevitably leads to land degradation in terms of its physical, chemical, and biological properties. The increasing presence of monocultures, reduced crop rotation, and excessive use of mineral nutrients, lead to several negative phenomena in such agroecosystems. Along with efforts to reduce energy consumption, and environmental pollution, intensify sustainable agriculture systems, and maintain biodiversity, the possibility of increasing the area under perennial forage legumes should be considered. As nitrogen fixers, these plants are minimally fertilized with nitrogen fertilizers whose residues in the soil are lost by leaching, causing pollution of groundwater as well as surface watercourses. The introduction of perennial legumes in the crop rotation can provide numerous benefits, such as increased and more stable yields of protein-rich biomass, conservation, and repair of land resources, increased yield stability, better utilization of nutrients, water, and light, as well as weed, disease, and pest control. The introduction of legumes in production systems would limit the increasingly pronounced land degradation. In order to develop sustainable agriculture, market policy should recognize the value of products obtained from leguminous plants through certain agricultural policy measures.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of harvest date on plant growth parameters and fruit phytochemical contents in different cultivars of chokeberry 采收期对不同品种蔓越莓植株生长参数及果实化学成分的影响

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313243

Nafiye ÜNAL, Mehmet A. SARIDAŞ, Erdal AĞÇAM, Asiye AKYILDİZ, Sevgi PAYDAŞ KARGI

{"title":"Effects of harvest date on plant growth parameters and fruit phytochemical contents in different cultivars of chokeberry","authors":"Nafiye ÜNAL, Mehmet A. SARIDAŞ, Erdal AĞÇAM, Asiye AKYILDİZ, Sevgi PAYDAŞ KARGI","doi":"10.15835/nbha51313243","DOIUrl":"https://doi.org/10.15835/nbha51313243","url":null,"abstract":"Chokeberry, a type of berry fruit, has garnered attention in recent years due to its remarkable antioxidant properties. The rich bioactive components found in its fruits have been found to exert positive effects on human health. In this research, the growth, yield, and quality of two chokeberry cultivars (‘Nero’ and ‘Viking’) were evaluated, along with detailed biochemical parameters of the fruits, across different harvest dates ranging from mid-August to mid-September. During the experiment, it was observed that the ‘Viking’ cultivar exhibited more upright and taller plant growth than ‘Nero’. There were no significant differences observed between the cultivars in terms of yield, total soluble solids (TSS), total phenolic content (TPC), total monomeric anthocyanins (TMA), and antioxidant activity. However, ‘Nero’ demonstrated superiority in phenolic acids (excluding ferulic acid), glucose, citric acid, quinic acid, and ascorbic acid content. As the harvest progressed from August 31 to September 15, there was an increase in the accumulation of fruit TSS, TPC, TMA, sucrose, glucose, fructose, sorbitol, epicatechin, neo-chlorogenic acid, caffeic acid, chlorogenic acid, and ferulic acid. Harvest dates did not impact antioxidant activity and anthocyanin compound values. Among the taste-related compounds, sorbitol was determined to be the most abundant, while sucrose exhibited a positive correlation with all individual sugars. Additionally, malic acid and quinic acid showed a positive correlation. The combination of the ‘Nero’ cultivar and the September 15th harvest date stood out as the most prominent in superior fruit bioactive compounds.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carboxylesterase from edible legume seeds and their sensitivity to organophosphate and carbamate pesticides 食用豆科植物种子羧酸酯酶及其对有机磷和氨基甲酸酯类农药的敏感性

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-08-31 DOI: 10.15835/nbha51313234

Chiradet NASUK, Monrodee CHAOWARAT, Sumonwan CHUMCHUERE

{"title":"Carboxylesterase from edible legume seeds and their sensitivity to organophosphate and carbamate pesticides","authors":"Chiradet NASUK, Monrodee CHAOWARAT, Sumonwan CHUMCHUERE","doi":"10.15835/nbha51313234","DOIUrl":"https://doi.org/10.15835/nbha51313234","url":null,"abstract":"Carboxylesterase from edible legume seeds, including black gram bean (Vigna mungo (L.) Hepper), mung bean (Vigna radiata (L.) Wilczek) and red bean (Vigna umbellata (Thunb.) Ohwi and Ohashi) has been partially purified using the ammonium sulphate salting-out procedure. The specific activity of the partially purified carboxylesterase extracted from black gram bean, mung bean and red bean are 3.96±0.26, 1.51±0.10 and 1.29±0.08 U/mg, respectively (p<0.05). The carboxylesterase from black gram bean showed very high sensitivity to 24 types of organophosphate pesticides (OPs) and 7 types of carbamate pesticides (CAs) at the concentrations of 0.001-3.0 µg/ml. The limit of detection (LOD) obtained for OPs and CAs pesticide residues were 0.002 and 0.01 mg/kg, respectively. For performance assessment of methods for pesticide determination in vegetables, all performance parameter values, including sensitivity, accuracy, and specificity of the carboxylesterase enzyme inhibition method, are 100% compared with commercial pesticide test kits and are 70.5%, 90%, and 100%, respectively, compared with chromatographic method analysis.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136035556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0