Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells

IF 1.4 4区生物学 Q3 PLANT SCIENCES

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-04 DOI:10.15835/nbha51312990

Benjaporn BURANRAT, Pornpimon WONGSUWAN, Wichuda PHIPHITPHIBUNSUK

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引用次数: 0

Abstract

Plumbago indica L. has a various pharmacological activity; however, the anticancer activity in cervical cancer has less information. To examine the antiproliferative, apoptotic, and antimigratory activity of Plumbago indica root extract on cervical cancer cells. The extract of P. indica was explored for cell viability, apoptosis, and migration by sulforhodamine B, colony formation, cell cycle distribution, Wound healing, Annexin V-FITC staining apoptotic, mitochondrial function, and reactive oxygen species (ROS) formation. The data indicated that P. indica extract suppressed HeLa cervical cells viability by dose- and time-dependent manner along with the reduction of cancer cell number and colony forming ability. After 48 h of incubation period, the PR extract exhibited a significant reduction of migratory ability by dose-dependent manner. Furthermore, these extracts significantly showed a reduction of cell viability along with induction of late apoptosis. The mechanism of action was shown by detecting mitochondrial dysfunction and activating of ROS formation on HeLa cells-treated with P. indica extract. The extract of P. indica has a growth inhibition, migratory suppression, and apoptotic induction. The extract has an anticancer activity by reducing mitochondrial function and activating ROS formation.

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梧桐根提取物对宫颈癌HeLa细胞的抗癌活性评价

印度梧桐具有多种药理活性;然而，对子宫颈癌的抗癌作用却知之甚少。探讨梧桐根提取物对宫颈癌细胞的抗增殖、抗凋亡和抗迁移活性。通过硫代丹B对P. indica提取物的细胞活力、凋亡和迁移、菌落形成、细胞周期分布、伤口愈合、Annexin V-FITC染色凋亡、线粒体功能和活性氧(ROS)形成进行了研究。结果表明，籼稻提取物具有剂量依赖性和时间依赖性，可抑制HeLa宫颈癌细胞活力，降低肿瘤细胞数量和集落形成能力。孵育48 h后，PR提取物的迁移能力呈剂量依赖性显著降低。此外，这些提取物显著降低细胞活力并诱导晚期凋亡。通过检测线粒体功能障碍和激活活性氧的形成，揭示了籼稻提取物对HeLa细胞的作用机制。其提取物具有抑制细胞生长、抑制细胞迁移和诱导细胞凋亡的作用。该提取物通过降低线粒体功能和激活ROS形成而具有抗癌活性。

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来源期刊

Notulae Botanicae Horti Agrobotanici Cluj-napoca PLANT SCIENCES-

CiteScore

2.70

自引率

0.00%

发文量

118

审稿时长

3 months

期刊介绍： Notulae Botanicae Horti Agrobotanici Cluj-Napoca is a peer-reviewed biannual journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (Notulae Botanicae Horti Agrobotanici Cluj-Napoca or JCR abbrev. title Not Bot Horti Agrobo) are mentioned.