Notulae Botanicae Horti Agrobotanici Cluj-napoca最新文献

{"title":"Efficiency of RAPD and SSR markers in assessing genetic diversity in summer onion (Allium cepa L.) genotypes","authors":"Priyadarshani P. MOHAPATRA, Mahmoud F. SELEIMAN, Rupsanatan MANDAL, Kartik PRAMANIK, Tapan Kumar MAITY, Jayanta TARAFDAR, Subhashree P. DAS, Chandini A. RAJ, Chinmaya JENA, Mekhled M. ALENAZI, Sumit SOW, Shivani RANJAN, Awais AHMAD, Harun GITARI, Naeem KHAN","doi":"10.15835/nbha51313369","DOIUrl":"https://doi.org/10.15835/nbha51313369","url":null,"abstract":"The genetic diversity assessment of agricultural crops is crucial for breeding programs aimed at enhancing crop yield, resistance to diseases, and adaptation to changing environmental conditions. In the present investigation, a comparative genetic relationship in sixteen onion genotypes was assessed utilizing dominant (RAPD) and co-dominant (SSR) marker systems. Ten RAPD and nine SSR markers showed genetic diversity remarkably and produced 503 and 107 amplicons respectively. Spearman rank correlation was used to compare the different efficiency parameters in two marker systems with respect to sixteen onion genotypes. The genetic relationship based on similarity matrix values between a pair of cultivars was higher for SSR markers than for the RAPD marker system. OPC-04 (RAPD primer) and ACM-004 (SSR primer) witnessed the highest poly-morphic bands along with other polymorphic markers that proved to be useful in grouping onion genotypes. Finally, dendrograms were constructed and compared following the mantel test to find out the genetic diversity among the germplasms. This study will be effective for a selection of efficient primers and suitable marker systems to distinguish the onion genotypes in the future.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134971707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly preparation of thyme essential oil nano emulsion: Characterization, antifungal activity and resistance of Fusarium wilt disease of Foeniculum vulgare","authors":"Mohamed S. ATTIA, Amer M. ABDELAZIZ, Mahmoud M.H. HASSANIN, Abdulaziz A. AL-ASKAR, Samy A. MAREY, Hamada ABDELGAWAD, Amr H. HASHEM","doi":"10.15835/nbha51313312","DOIUrl":"https://doi.org/10.15835/nbha51313312","url":null,"abstract":"Essential oil nanoemulsions have received much attention in the last period for controlling of fungal plant pathogens. In this study, thyme oil nanoemulsion (TONE) was successfully prepared from thyme oil which extracted from Thymus vulgaris (T. vulgaris). The prepared TONE was characterized using DLS, Zeta potential, and TEM analyses. Results revealed that, TONE has spherical shape with size 32.7 nm. Moreover, results illustrated that TONE exhibited promising antifungal activity against Fusarium oxysporum (F. oxysporum) with minimum fungicidal concentration (MFC) 5 mg/ml. Additionally, TONE concentrations 1, 2, 3 and 4 mg/ml reduced the growth of F. oxysporum with percentages 7.78, 31.1, 52.2 and 67.8 % respectively. Disease index (DI) of Fusarium wilt reached the maximum rate by (85 %) in the Foeniculum vulgare (F. vulgare) plant infected with F. oxysporum. Application of TONE treatment on infected plants led to a decrease in DI to (17.5%) and an increase in the percentage of protection to (79.4%). Furthermore, DI was decrease to 42.5% with protection percentage 50% in the case of infected plant with TOE. Moreover, TOE, TONE played an important role in improving plant immunity by increasing phenol, proline, and antioxidant enzymes (POD&PPO) activities, as well as reducing oxidative stress by reducing (MDA & H2O2). Results revealed that TONE led to significant increase in free proline in compared to TOE. We can conclude that TOE, TONE are considered eco-friendly safe strong inducers of F. vulgare plant immunity alternatives to difenoconazole against fusarial wilt disease to preserve plant, soil, and human health.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135487471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective evaluation of Marsilea quadrifolia L. and Salvinia molesta D.S Mitchel in aluminium chloride induced Alzheimer disease","authors":"Sadia MUSHTAQ, Uzma HANIF, Muhammad Ajmal SHAH, Nazish MAZHAR, Zunera CHAUHDARY, Muhammad QASIM, Muhamamd MUNEEB, Maham CHAUDHARY, Marium DAR","doi":"10.15835/nbha51313228","DOIUrl":"https://doi.org/10.15835/nbha51313228","url":null,"abstract":"Phenolic compounds are very effective for the cure of various neurological and pathological diseases. Current study was conducted to estimate the Phenolic compounds through HPLC and to perform the anti-Alzheimer activity from two aquatic Leptosporangiate ferns; Marsilea quadrifolia L. and Salvinia molesta D. Crushed leaves were subjected to Microwave Assisted Extraction to prepare plant leave ethanolic extract of plants under study. Aluminum chloride induced Alzheimer’s disease model in rats by estimating behavioural and biochemical parameters were measured for ethanolic extract of both plants. Different behavioural tests were performed for neuromuscular coordination estimation and hole board test for exploratory behaviour were performed. The levels of AChE inhibitory activity and antioxidant enzymes were estimated on brain homogenates and Histopathological analysis were also performed. Phenolic compounds of leaf extracts were noted for HPLC chromatograms of standard compounds with Gallic acid (33.76 ± 0.08) higher in M. quadrifolia L. and Salicylic acid (12.45 ± 0.07) higher in S. molesta D. S Mitchel. Histopathological analysis showed that M. quadrifolia and S. molesta reversed the neurofibrillary tangles, degenerative changes, neuronal loss and neuroinflammation in the brain architecture. Both ferns at a dose of 500 mg/kg and 1000mg/kg significantly improved the Aluminium chloride induced neurotoxicity by modulating cognitive disabilities and motor dysfunctions, and normalized the biochemical alteration of acetylcholinesterase and antioxidant enzymes. In conclusion, both plants M. quadrifolia and S. molesta improved behavioural and biochemical parameters and therefore will be effective in treatment of neurodegenerative disorders via countless underlying mechanistic pathways.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136026009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical, bioactive compounds and antioxidant activities of sweet red corn under different drying methods","authors":"Onanong PHUSEERIT, Theeraphan CHUMROENPHAT","doi":"10.15835/nbha51313301","DOIUrl":"https://doi.org/10.15835/nbha51313301","url":null,"abstract":"This study investigated the physicochemical properties, bioactive compounds, and antioxidant activities of sweet red corn under different drying methods. The color characteristics of the corn varied significantly based on the drying method employed. Freeze-drying resulted in the lightest color, while sun drying and hot air drying at different temperatures showed intermediate and varying color parameters. The total phenolic and flavonoid content decreased after drying, with heat drying methods exhibiting a greater decrease compared to freeze-drying. Microstructural analysis revealed changes in cell structure and starch granules, with freeze-dried samples displaying distinct characteristics. The sugar content decreased in all drying methods compared to the fresh sample. The amino acid content also decreased after drying, with heat drying methods affecting certain amino acids more than others. Different phenolic acids and flavonoids were detected, with their concentrations varying depending on the drying method. Fresh corn exhibited the highest antioxidant activity, while freeze-drying retained a significant amount. Sun drying and high-temperature drying methods resulted in decreased antioxidant activity. These findings underscore the importance of selecting appropriate drying techniques to preserve desired attributes and beneficial properties of corn.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136026008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and anti-inflammatory effect of Phellodendron amurense Rupr. stem bark extract","authors":"Sabina-Emanuela ERHAN, Alina E. PÂRVU, Alexandra CIORÎȚĂ, Ayu A. PUTRI, Alexis J. VILLAGRANA MOLINA, Marcel PÂRVU, Augustin C. MOȚ","doi":"10.15835/nbha51313306","DOIUrl":"https://doi.org/10.15835/nbha51313306","url":null,"abstract":"In many diseases inflammation and oxidative stress coexist and are therapeutic targets. Phellodendron amurense Rupr. has anti-inflammatory and antioxidant effects, but the mechanisms are not completely elucidated. Therefore, first P. amurense stem bark extract was analysed regarding chemical compounds such as total polyphenol content (TPC), polyphenols (gallic acid, 4-hydroxybenzoic acid, caffeic acid and ferulic acid) and alkaloids (berbamine, jatrorrhizine, palmatine, and berberine). Quantitative determination of alkaloids revealed that berberine had the highest concentration of 2.44±0.22 mg/g. In vitro antioxidant activity was significant. Then, thein vivo anti-inflammatory and antioxidative effects of P. amurense extract were studied before and after acute experimental rat inflammation induction in order to find some mechanisms of these effects. The P. amurense stem bark extract had a good anti-inflammatory activity by reducing nitric oxide, 3-nitrotyrosine and NF-kB, and an antioxidant activity by lowering oxidants and increasing antioxidants. This study provides scientific knowledge and could contribute to the development of novel drugs based on P. amurense stem bark extract for the treatment of inflammatory diseases and prevention of oxidative stress.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136026015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulating the antioxidant defense systems and nutrients content by proline for higher yielding of wheat under water deficit","authors":"Maha L. HADID, Khaled M.A. RAMADAN, Hossam S. EL-BELTAGI, Amany A. RAMADAN, Ibrahim M. EL-METWALLY, Tarek A. SHALABY, Eslam S.A. BENDARY, Khairiah MUBARAK ALWUTAYD, Hani S. SAUDY","doi":"10.15835/nbha51313291","DOIUrl":"https://doi.org/10.15835/nbha51313291","url":null,"abstract":"Numerous plant metabolites, especially amino acids, are accumulated as a result of stress. These amino acids are crucial for plant metabolism and development and have historically been viewed as the building blocks of proteins. Several studies suggested that there is a link between proline buildup and exposure plants to stress. Proline performs important functions under stress in addition to be a great osmolyte, antioxidant enzyme, acts as an antioxidant defense and signaling molecules. Two field trials were during two successive winter seasons (2020/2021 and 2021/2022). The effects of proline foliar application (0, 100, 200, 300 and 400 mg L−1) and irrigation water levels (irrigation by 100, 80 and 60% of crop water requirements, CWR, CWR100, CWR80 and CWR60, respectively) on plant pigments, antioxidants activity, yield traits and nutrient contents of wheat were assessed. The experiment was designed in a split-plot involving 4 replicates. Drought stress (CWR80 and CWR60) led to reductions in photosynthetic pigments, and yield components. Under severe water stress (CWR60), proline 200 and 300 mg L−1 recorded the highest values of chlorophyll a, chlorophyll b, and total pigments. Application of proline 300 mg L−1 was the potent practice for enhancing the antioxidant activity% (DPPH radical scavenging) and phenols content under CWR100 and CWR80. Indole acetic acid (IAA) possessed the maximum values with proline 200 mg L−1 under all irrigation patterns. Under severe deficit water (CWR60), without proline spraying (for super oxide dismutase), 200, 300 or 400 mg L−1 proline (for peroxidase) and 200 or 400 mg L−1 proline (for polyphenol oxidase) recorded the highest values. Spraying proline 300 mg L−1 achieved the highest values of grain number spike−1, weight of 1000 grains and grain yield ha−1 under different irrigation regimes. In conclusion, proline is considered a good mitigator for drought stress, due to it increased wheat plant tolerance to water deficiency throughout improving plant physiology and consequently yields quantity and quality. The most efficient concentrations of proline for coping the adverse impact of drought were 200 and 300 mg L−1.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136364070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical properties, antioxidant potential and fatty acids profiling of three dragon fruit species grown under sub-tropical climate","authors":"Jaswinder S. BRAR, Sanjula SHARMA, Harjeevan KAUR, Harpreet SINGH, E.K. NAIK, Trina ADHIKARY","doi":"10.15835/nbha51312993","DOIUrl":"https://doi.org/10.15835/nbha51312993","url":null,"abstract":"The physical, biochemical and antioxidant properties of one white pulped (Hylocereus undatus; DG-I) and two red pulped dragon fruit species (H. polyrhizus, DG-II; H. costaricencis, DG-III) grown under sub-tropical conditions of north-west India were determined. Fruit size, fruit weight, pulp weight and pulp: peel ratio was significantly higher in DG-III, though the fruit numbers and yield per pillar was significantly less than other species. The pH, TSS, acidity, total sugar, reducing sugars and moisture content in all the species varied between 4.78-5.72, 8.63-9.31 oBrix, 0.30-0.56%, 6.64-6.91%, 4.60-4.76% and 83.44-85.82%, respectively. Total phenols and flavanols content in DG-I was 24.04 mg GAE 100 g−1 and 14.54 mg RE 100 g−1, whereas in red pulped it was significantly higher; varying between 49.12-56.40 mg GAE 100 g−1 and 30.41-31.10 mg RE 100 g−1 fruit pulp, respectively. β-carotene values in red pulped species DG-II and DG-III were 47.48 and 43.82 µg 100 g−1, respectively compared to corresponding values of 1.96 µg 100 g−1 in DG-I, a white pulped dragon fruit. Similarly, DPPH-RSC, FRAP, CUPRAC and ABTS values for red pulped dragon fruit ranged between 238.98-262.04 µmol 100 g−1, 358.8-386.40 µmol TE 100 g−1, 830.40-917.0 µmol TE 100 g−1 and 571.4-589.60 µmol 100 g−1, respectively in DG-II and DG-III in comparison to respective values of 108.75 µmol 100 g−1, 192.6 µmol TE 100 g−1, 525.6 µmol TE 100 g−1 and 400.2 µmol 100 g−1 in DG-I. The β-lain, responsible for imparting red colour in DG-II and DG-III was absent in white pulped DG-I. Seed oil content in both groups of dragon fruit varied between 31.90-33.5% with highest proportion of an essential fatty acid, linoleic acid (46.32-47.96%). In conclusion, red pulped dragon fruit has a considerably higher antioxidative potential than white one and these species may play a vital role in ensuring nutritional security for millions of people in developing nations.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135205743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A different factor in the use of plants in landscape architecture: Sound (type, intensity and duration) in the example of Hyacinthus orientalis L.","authors":"Arzu ÇIĞ, Arzu KOÇAK MUTLU, Nazire MİKAİL","doi":"10.15835/nbha51313271","DOIUrl":"https://doi.org/10.15835/nbha51313271","url":null,"abstract":"The effect of music on people has been known for years and is still being researched from different aspects. The effects of music and sound waves on ornamental plants, whose effects on some vegetables, fruits and grains are examined, are also inquired. Especially the positive change in the development and showiness of the flowers of ornamental plant species with commercial importance will increase the market value of the plant. Again, with the effect of this sound wave, in order for the plants and their flowers to show the expected development, they should benefit from the planting environment and growing conditions at the maximum level. In the measurements taken from hyacinths (Hyacinthus orientalis L.) at the end of the duration that the plants were exposed to different types of sounds in different intensities, it was observed that these factors positively affected these parameters successively; 1 hour of bird sound in 50 dB, the number of leaves; 1 hour of bird sound in 90 dB, leaf width and floret length; 3 hours of bird sound in 70 dB, floret number; 3 hours of bird sound in 90 dB, the plant and flower height; 1 hour of bee sound in 50 dB, the stem thickness; 3 hours of vehicle sound in 50 dB, flower and floret width; 3 hours of vehicle sound in 70 dB, leaf length. At the end of the study, whereas it was determined that the bee sound had the least effect on the growth and flowering of the hyacinth, it was observed that the bird and vehicle sounds, that the plants were expose to in different intensities and durations, had a positive effect.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethnobotany of Hmong ethnic groups in Bolikhamxay province, central Laos PDR","authors":"Kajonesuk PHENGMALA, Surapon SAENSOUK, Piyaporn SAENSOUK, Phetlasy SOULADETH","doi":"10.15835/nbha51313284","DOIUrl":"https://doi.org/10.15835/nbha51313284","url":null,"abstract":"Results from an ethnobotany study of Hmong ethnic groups in Bolikhamxay Province, Laos PDR between 2021 and 2023 identifies plant uses for 133 species, 104 genera, and 50 families. The research focusses on specific plant uses. Quantitative analyses are applied with Use Value (UV), Fidelity Level (FL), and Informant Agreement Ratio (IAR). Cluster analysis based on the Jaccard’s Similarity Index (JI) are calculated for the similarity of edible plant used in three areas as follows: cultivated, from the forest, and in the market. The most important plant species are Imperata cylindrica (L.) Raeusch. with a UV of 3.45 followed by Calamus viminalis Willd. (2.85) and Curcuma zedoaria (Christm.) Roscoe (2.83). The highest FL value for 23 plant species with 100% FL. The highest consensus within ailment categories is for urinary system symptoms, neurological symptoms and oral syndrome (IAR=1.00). The JI varies between 0.1013 and 0.2981; the highest JI is for pairs of cultivated and in the market. UPGMA cluster analysis indicates that the plants are collected from isolated forests with low similarity to other areas. Considering the numerous useful plants that have been documented, along with the identification of culturally and historically significant species, as well as their distribution and conservation status, it becomes evident that forest conservation is crucial for preserving the livelihoods and associated ethnobotanical knowledge of the local and indigenous people in Bolikhamxay Province.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of herbaceous vegetation classification using orthophotos produced from the image acquired with unmanned aerial systems","authors":"Sudeera WICKRAMARATHNA, John GOETZ III, Jon SOUDER, Benjamin PROTZMAN, Brian SHEPARD, Sorin HERBAN, Francisco MAURO, Hailemariam TEMESGEN, Bogdan M. STRIMBU","doi":"10.15835/nbha51313227","DOIUrl":"https://doi.org/10.15835/nbha51313227","url":null,"abstract":"Arguably the most popular remote-sensing products are classified images. However, there are no definitive procedures to assess classification accuracy that simultaneously consider resources available and field efforts. The explosive usage of unmanned aerial systems (UAS) in land surveys adds new challenges to classification assessment, as orthorectified images usually contain significant artifacts. This study aims to identify the optimal ratio between training and validation sample size within a supervised classification approach applied to UAS orthophotos. As a case study, we used a wetland area west of Portland, OR, USA, treated with various glyphosate formulations to control Phalaris arundinacea, commonly known as reed canary grass. A completely randomized design with five replications and six glyphosate formulations was used to assess P. arundinacea vigor following repeated herbicide applications. The change in P. arundinacea vitality was monitored with high-resolution four-band imagery acquired with a SlantRange 3PX camera installed on a DJI Matrice 210. The orthophotos created from images were produced with Pix4D, which was subsequently preprocessed with ERDAS Imagine 2020 to reduce the noise, shadows, and artifacts. All images were classified with the maximum likelihood classification algorithm. Simple random and stratified random sampling methods were applied to collect training and validation samples, evaluating eight ratios of training to validation samples to assess their classification accuracy. We found that increasing the training-to-validation sample size ratio enhances accuracy, with the 3:1 ratio being the most reliable in classifying P. arundinacea vigor. Our study provides evidence that image preprocessing and enhancement are essential for UAS-based imagery.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135364434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}