Efficiency of RAPD and SSR markers in assessing genetic diversity in summer onion (Allium cepa L.) genotypes

IF 1.4 4区生物学 Q3 PLANT SCIENCES

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-14 DOI:10.15835/nbha51313369

Priyadarshani P. MOHAPATRA, Mahmoud F. SELEIMAN, Rupsanatan MANDAL, Kartik PRAMANIK, Tapan Kumar MAITY, Jayanta TARAFDAR, Subhashree P. DAS, Chandini A. RAJ, Chinmaya JENA, Mekhled M. ALENAZI, Sumit SOW, Shivani RANJAN, Awais AHMAD, Harun GITARI, Naeem KHAN

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引用次数: 0

Abstract

The genetic diversity assessment of agricultural crops is crucial for breeding programs aimed at enhancing crop yield, resistance to diseases, and adaptation to changing environmental conditions. In the present investigation, a comparative genetic relationship in sixteen onion genotypes was assessed utilizing dominant (RAPD) and co-dominant (SSR) marker systems. Ten RAPD and nine SSR markers showed genetic diversity remarkably and produced 503 and 107 amplicons respectively. Spearman rank correlation was used to compare the different efficiency parameters in two marker systems with respect to sixteen onion genotypes. The genetic relationship based on similarity matrix values between a pair of cultivars was higher for SSR markers than for the RAPD marker system. OPC-04 (RAPD primer) and ACM-004 (SSR primer) witnessed the highest poly-morphic bands along with other polymorphic markers that proved to be useful in grouping onion genotypes. Finally, dendrograms were constructed and compared following the mantel test to find out the genetic diversity among the germplasms. This study will be effective for a selection of efficient primers and suitable marker systems to distinguish the onion genotypes in the future.

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RAPD和SSR标记在夏葱基因型遗传多样性评价中的应用

农作物遗传多样性评估对于提高作物产量、抗病性和适应不断变化的环境条件的育种计划至关重要。本研究利用显性(RAPD)和共显性(SSR)标记系统对16个洋葱基因型的比较亲缘关系进行了评价。10个RAPD和9个SSR标记表现出显著的遗传多样性，分别产生503个和107个扩增子。采用Spearman秩相关法比较了16个洋葱基因型在两种标记体系中的不同效率参数。基于相似性矩阵值的亲缘关系在SSR标记体系中高于RAPD标记体系。OPC-04 (RAPD引物)和ACM-004 (SSR引物)以及其他多态性标记显示多态性条带最高，这些标记被证明对洋葱基因型分类有用。最后，通过树突图的构建，比较了各种质间的遗传多样性。本研究为今后选择有效的引物和合适的标记体系来区分洋葱基因型提供了依据。

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来源期刊

Notulae Botanicae Horti Agrobotanici Cluj-napoca PLANT SCIENCES-

CiteScore

2.70

自引率

0.00%

发文量

118

审稿时长

3 months

期刊介绍： Notulae Botanicae Horti Agrobotanici Cluj-Napoca is a peer-reviewed biannual journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (Notulae Botanicae Horti Agrobotanici Cluj-Napoca or JCR abbrev. title Not Bot Horti Agrobo) are mentioned.