npj Aging最新文献_第3页

Quantification of localized NAD⁺ changes reveals unique specificity of NAD⁺ regulation in the hypothalamus. 对局部 NAD+ 变化的定量分析揭示了下丘脑中 NAD+ 调节的独特特异性。

npj Aging Pub Date : 2023-01-25 DOI: 10.1038/s41514-023-00098-1

Sean Johnson, Kiyoshi Yoshioka, Cynthia S Brace, Shin-Ichiro Imai

{"title":"Quantification of localized NAD+ changes reveals unique specificity of NAD+ regulation in the hypothalamus.","authors":"Sean Johnson, Kiyoshi Yoshioka, Cynthia S Brace, Shin-Ichiro Imai","doi":"10.1038/s41514-023-00098-1","DOIUrl":"10.1038/s41514-023-00098-1","url":null,"abstract":"Recently, it has become a consensus that systemic decreases in NAD+ are a critical trigger for age-associated functional decline in multiple tissues and organs. The hypothalamus, which contains several functionally distinct subregions called nuclei, functions as a high-order control center of aging in mammals. However, due to a technical difficulty, how NAD+ levels change locally in each hypothalamic nucleus during aging remains uninvestigated. We were able to establish a new combinatorial methodology, using laser-captured microdissection (LCM) and high-performance liquid chromatography (HPLC), to accurately measure NAD+ levels in small tissue samples. We applied this methodology to examine local NAD+ changes in hypothalamic nuclei and found that NAD+ levels were decreased significantly in the arcuate nucleus (ARC), ventromedial hypothalamus (VMH), and lateral hypothalamus (LH), but not in the dorsomedial hypothalamus (DMH) of 22-month-old mice, compared to those of 3-month-old mice. The administration of nicotinamide mononucleotide (NMN) significantly increased NAD+ levels in all these hypothalamic nuclei. Interestingly, the administration of extracellular nicotinamide phosphoribosyltransferase-containing extracellular vesicles (eNampt-EVs) purified from young mice increased NAD+ levels in the ARC and DMH. These results reveal the unique specificity of NAD+ regulation in the hypothalamus during aging.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9135260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple pathways promote microtubule stabilization in senescent intestinal epithelial cells. 多种途径促进衰老肠上皮细胞的微管稳定。

npj Aging Pub Date : 2022-12-16 DOI: 10.1038/s41514-022-00097-8

Siwei Chu, Ossama Moujaber, Serge Lemay, Ursula Stochaj

{"title":"Multiple pathways promote microtubule stabilization in senescent intestinal epithelial cells.","authors":"Siwei Chu, Ossama Moujaber, Serge Lemay, Ursula Stochaj","doi":"10.1038/s41514-022-00097-8","DOIUrl":"https://doi.org/10.1038/s41514-022-00097-8","url":null,"abstract":"Intestinal epithelial cells are critical for gastrointestinal homeostasis. However, their function declines during aging. The aging-related loss of organ performance is largely driven by the increase in senescent cells. To date, the hallmarks and molecular mechanisms related to cellular senescence are not fully understood. Microtubules control epithelial functions, and we identified microtubule stabilization as a phenotypic marker of senescent intestinal epithelial cells. The senescence inducer determined the pathway to microtubule stabilization. Specifically, enhanced microtubule stability was associated with α-tubulin hyperacetylation or increased abundance of the microtubule-binding protein tau. We show further that overexpression of MAPT, which encodes tau, augmented microtubule stability in intestinal epithelial cells. Notably, pharmacological microtubule stabilization was sufficient to induce cellular senescence. Taken together, this study provides new insights into the molecular mechanisms that control epithelial cell homeostasis. Our results support the concept that microtubule stability serves as a critical cue to trigger intestinal epithelial cell senescence.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10399664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of potential blood biomarkers for early diagnosis of Alzheimer's disease through immune landscape analysis. 通过免疫景观分析鉴定早期诊断阿尔茨海默病的潜在血液生物标志物。

npj Aging Pub Date : 2022-11-04 DOI: 10.1038/s41514-022-00096-9

Daichi Shigemizu, Shintaro Akiyama, Risa Mitsumori, Shumpei Niida, Kouichi Ozaki

{"title":"Identification of potential blood biomarkers for early diagnosis of Alzheimer's disease through immune landscape analysis.","authors":"Daichi Shigemizu, Shintaro Akiyama, Risa Mitsumori, Shumpei Niida, Kouichi Ozaki","doi":"10.1038/s41514-022-00096-9","DOIUrl":"https://doi.org/10.1038/s41514-022-00096-9","url":null,"abstract":"Mild cognitive impairment (MCI) is a clinical precursor of Alzheimer's disease (AD). Recent genetic studies have reported on associations between AD risk genes and immunity. Here, we obtained samples and data from 317 AD, 432 MCI, and 107 cognitively normal (CN) subjects and investigated immune-cell type composition and immune clonal diversity of T-cell receptor (TRA, TRB, TRG, and TRD) and B-cell receptor (IGH, IGK, and IGL) repertoires through bulk RNA sequencing. We found the proportions of plasma cells, γδ T cells, neutrophils, and B cells were significantly different and the diversities of IGH, IGK, and TRA were significantly small with AD progression. We then identified a differentially expressed gene, WDR37, in terms of risk of MCI-to-AD conversion. Our prognosis prediction model using the potential blood-based biomarkers for early AD diagnosis, which combined two immune repertoires (IGK and TRA), WDR37, and clinical information, successfully classified MCI patients into two groups, low and high, in terms of risk of MCI-to-AD conversion (log-rank test P = 2.57e-3). It achieved a concordance index of 0.694 in a discovery cohort and of 0.643 in an independent validation cohort. We believe that further investigation, using larger sample sizes, will lead to practical clinical use in the near future.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636153/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40447646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Author Correction: Pyrroloquinoline quinone (PQQ) protects mitochondrial function of HEI-OC1 cells under premature senescence. 作者更正:吡咯喹啉醌(PQQ)保护HEI-OC1细胞在过早衰老下的线粒体功能。

npj Aging Pub Date : 2022-10-06 DOI: 10.1038/s41514-022-00095-w

Ying Gao, Teru Kamogashira, Chisato Fujimoto, Shinichi Iwasaki, Tatsuya Yamasoba

引用次数: 0

Why do older adults stand-up differently to young adults?: investigation of compensatory movement strategies in sit-to-walk. 为什么老年人和年轻人的单口喜剧表演方式不同?:坐姿-步行代偿运动策略的研究。

npj Aging Pub Date : 2022-09-05 DOI: 10.1038/s41514-022-00094-x

Eline van der Kruk, Paul Strutton, Louis J Koizia, Michael Fertleman, Peter Reilly, Anthony M J Bull

{"title":"Why do older adults stand-up differently to young adults?: investigation of compensatory movement strategies in sit-to-walk.","authors":"Eline van der Kruk, Paul Strutton, Louis J Koizia, Michael Fertleman, Peter Reilly, Anthony M J Bull","doi":"10.1038/s41514-022-00094-x","DOIUrl":"https://doi.org/10.1038/s41514-022-00094-x","url":null,"abstract":"Functional motor redundancy enables humans to move with distinct muscle activation patterns while achieving a similar outcome. Since humans select similar strategies, there seems to be an optimal control. However, older adults move differently to young adults. The question is whether this is this due to an altered reinforcement scheme, altered sensory inputs, or due to alterations in the neuromusculoskeletal systems, so that it is no longer optimal or possible to execute the same movement strategies. The aim of this study was to analyse natural compensation strategies in the vital daily-life-task, sit-to-walk, in relation to neuromuscular capacity and movement objectives in younger (27.2 ± 4.6 years, N = 27, 14♀) and elderly (75.9 ± 6.3 years, N = 23, 12♀) adults. Aspects of the neuromuscular system that are prone to age-related decline and feasible to quantify were assessed (i.e. strength, nerve conductivity, fear of falling). Kinematics and muscle activity were recorded and joint kinetics were estimated using biomechanical models. Elderly men consistently used their arms when standing up. This strategy was not associated with a lack of or a reduction in strength, but with a reduction, but no lack of, ankle joint range of motion, and with increased fear of falling. The results show that humans preferentially maintain a minimum threshold of neuromuscular reserve to cope with uncertainties which results in compensation prior to coming up against physical limitations. Smaller base of support while standing up, a compensatory strategy with possibly greater risk of falls, was associated with muscular weakness, and longer nerve conduction latencies.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9445096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40351818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Integrated cognitive and physical fitness training enhances attention abilities in older adults. 综合认知和体能训练提高老年人的注意力能力。

npj Aging Pub Date : 2022-08-30 DOI: 10.1038/s41514-022-00093-y

Joaquin A Anguera, Joshua J Volponi, Alexander J Simon, Courtney L Gallen, Camarin E Rolle, Roger Anguera-Singla, Erica A Pitsch, Christian J Thompson, Adam Gazzaley

{"title":"Integrated cognitive and physical fitness training enhances attention abilities in older adults.","authors":"Joaquin A Anguera, Joshua J Volponi, Alexander J Simon, Courtney L Gallen, Camarin E Rolle, Roger Anguera-Singla, Erica A Pitsch, Christian J Thompson, Adam Gazzaley","doi":"10.1038/s41514-022-00093-y","DOIUrl":"https://doi.org/10.1038/s41514-022-00093-y","url":null,"abstract":"Preserving attention abilities is of great concern to older adults who are motivated to maintain their quality of life. Both cognitive and physical fitness interventions have been utilized in intervention studies to assess maintenance and enhancement of attention abilities in seniors, and a coupling of these approaches is a compelling strategy to buttress both cognitive and physical health in a time- and resource-effective manner. With this perspective, we created a closed-loop, motion-capture video game (Body-Brain Trainer: BBT) that adapts a player's cognitive and physical demands in an integrated approach, thus creating a personalized and cohesive experience across both domains. Older adults who engaged in two months of BBT improved on both physical fitness (measures of blood pressure and balance) and attention (behavioral and neural metrics of attention on a continuous performance task) outcome measures beyond that of an expectancy matched, active, placebo control group, with maintenance of improved attention performance evidenced 1 year later. Following training, the BBT group's improvement on the attention outcome measure exceeded performance levels attained by an untrained group of 20-year olds, and showed age-equilibration of a neural signature of attention shown to decline with age: midline frontal theta power. These findings highlight the potential benefits of an integrated, cognitive-physical, closed-loop training platform as a powerful tool for both cognitive and physical enhancement in older adults.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9427998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40329930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Age-dependent effects of blue light exposure on lifespan, neurodegeneration, and mitochondria physiology in Drosophila melanogaster. 蓝光照射对黑腹果蝇寿命、神经变性和线粒体生理的年龄依赖性影响。

npj Aging Pub Date : 2022-07-27 DOI: 10.1038/s41514-022-00092-z

Yujuan Song, Jun Yang, Alexander D Law, David A Hendrix, Doris Kretzschmar, Matthew Robinson, Jadwiga M Giebultowicz

{"title":"Age-dependent effects of blue light exposure on lifespan, neurodegeneration, and mitochondria physiology in Drosophila melanogaster.","authors":"Yujuan Song, Jun Yang, Alexander D Law, David A Hendrix, Doris Kretzschmar, Matthew Robinson, Jadwiga M Giebultowicz","doi":"10.1038/s41514-022-00092-z","DOIUrl":"https://doi.org/10.1038/s41514-022-00092-z","url":null,"abstract":"Blue light is a predominant component of light emitting devices (LEDs), which are increasingly present in our environment. There is already accumulating evidence that blue light exposure causes damage to retinal cells in vitro and in vivo; however, much less is known about potential effects of blue light on non-retinal cells. That blue light may be detrimental at the organismal level independent from retinal effect was recently shown by findings that it reduces lifespan in worms and also in flies with genetically ablated retinas. Here, we investigated the effects of blue light exposure across the fly lifespan and found that susceptibility to blue light stress is strongly age-dependent. The blue light of the same intensity and duration reduced survival and increased neurodegeneration more significantly in old flies than in young flies. These differences appear to be caused, at least in part, by impairments of mitochondrial respiratory function. We report that blue light significantly reduces the activity of Complex II in the electron transport system and decrease the biochemical activity of succinate dehydrogenase in both young and old flies. In addition, complex I and complex IV activities are reduced by age, as are ATP levels. We therefore propose that older flies are more sensitive to blue light because the light-induced mitochondrial damage potentiates the age-related impairments in energy metabolism that occurs even in darkness. Taken together, our results show that damaging effects of blue light at the organismal level are strongly age dependent and are associated with reduced activity of specific components of energy producing pathways in mitochondria.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40694313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Paracrine-mediated rejuvenation of aged mesenchymal stem cells is associated with downregulation of the autophagy-lysosomal pathway. 旁分泌激素介导的衰老间充质干细胞返老还童与自噬-溶酶体通路的下调有关。

npj Aging Pub Date : 2022-07-18 DOI: 10.1038/s41514-022-00091-0

George Hung, Tamara Ashvetiya, Aleksandra Leszczynska, Wanjun Yang, Chao-Wei Hwang, Gary Gerstenblith, Andreas S Barth, Peter V Johnston

{"title":"Paracrine-mediated rejuvenation of aged mesenchymal stem cells is associated with downregulation of the autophagy-lysosomal pathway.","authors":"George Hung, Tamara Ashvetiya, Aleksandra Leszczynska, Wanjun Yang, Chao-Wei Hwang, Gary Gerstenblith, Andreas S Barth, Peter V Johnston","doi":"10.1038/s41514-022-00091-0","DOIUrl":"https://doi.org/10.1038/s41514-022-00091-0","url":null,"abstract":"Age-related differences in stem-cell potency contribute to variable outcomes in clinical stem cell trials. To help understand the effect of age on stem cell potency, bone marrow-derived mesenchymal stem cells (MSCs) were isolated from young (6 weeks) and old (18-24 months) mice. HUVEC tubule formation (TF) induced by the old and young MSCs and ELISA of conditioned media were compared to one another, and to old MSCs after 7 d in indirect co-culture with young MSCs. Old MSCs induced less TF than did young (1.56 ± 0.11 vs 2.38 ± 0.17, p = 0.0003) and released lower amounts of VEGF (p = 0.009) and IGF1 (p = 0.037). After 7 d in co-culture with young MSCs, TF by the old MSCs significantly improved (to 2.09 ± 0.18 from 1.56 ± 0.11; p = 0.013), and was no longer different compared to TF from young MSCs (2.09 ± 0.18 vs 2.38 ± 0.17; p = 0.27). RNA seq of old MSCs, young MSCs, and old MSCs following co-culture with young MSCs revealed that the age-related differences were broadly modified by co-culture, with the most significant changes associated with lysosomal pathways. These results indicate that the age-associated decreased paracrine-mediated effects of old MSCs are improved following indirect co-culture with young MSC. The observed effect is associated with broad transcriptional modification, suggesting potential targets to both assess and improve the therapeutic potency of stem cells from older patients.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9293998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40582176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent cognitive impairment, hydrocephalus and leukocyte infiltration in transgenic mice with endothelial expression of human EPHX2. 人 EPHX2 内皮表达转基因小鼠随年龄增长而出现的认知障碍、脑积水和白细胞浸润。

npj Aging Pub Date : 2022-07-05 DOI: 10.1038/s41514-022-00090-1

Catherine M Davis, Wenri H Zhang, Thierno M Bah, Natalie E Roese, Elyse M Allen, Philberta Leung, Sydney J Boutros, Tessa Marzulla, Esha Patel, Xiao Nie, Farah N Alkayed, Justin H Huang, Michael A Jensen, Jacob Raber, Martin M Pike, Nabil J Alkayed

{"title":"Age-dependent cognitive impairment, hydrocephalus and leukocyte infiltration in transgenic mice with endothelial expression of human EPHX2.","authors":"Catherine M Davis, Wenri H Zhang, Thierno M Bah, Natalie E Roese, Elyse M Allen, Philberta Leung, Sydney J Boutros, Tessa Marzulla, Esha Patel, Xiao Nie, Farah N Alkayed, Justin H Huang, Michael A Jensen, Jacob Raber, Martin M Pike, Nabil J Alkayed","doi":"10.1038/s41514-022-00090-1","DOIUrl":"10.1038/s41514-022-00090-1","url":null,"abstract":"Soluble epoxide hydrolase (sEH) is upregulated in microvascular endothelium of human brain with vascular cognitive impairment (VCI). Transgenic endothelial expression of human sEH in mice (Tie2hsEH) induces endothelial dysfunction (ED), a pathogenetic mechanism of VCI. We sought to determine if endothelial upregulation of sEH is sufficient to cause cognitive impairment, and if cognitive impairment due to chronic hypoperfusion induced by unilateral common carotid artery occlusion (CCAO) is exacerbated in Tie2hsEH mice. Behavioral performance was assessed by the open field, rotarod, novel object, Morris water maze and fear conditioning tests. Cerebral blood flow and brain morphology were evaluated by MRI, and inflammatory changes investigated using immunohistochemistry and flow cytometry. We demonstrate that transgenic endothelial expression of sEH is sufficient to induce cognitive impairment, associated with leukocyte infiltration, brain atrophy and accelerated, age-dependent ventriculomegaly, identifying ED and sEH upregulation as potential underlying mechanisms and therapeutic targets for VCI.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9768200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice. 两种运动训练方案对雄性小鼠骨骼肌和肝脏基因组和代谢重塑的影响与年龄有关。

npj Aging Pub Date : 2022-06-27 DOI: 10.1038/s41514-022-00089-8

Michel Bernier, Ignacio Navas Enamorado, Mari Carmen Gómez-Cabrera, Miguel Calvo-Rubio, Jose Antonio González-Reyes, Nathan L Price, Ana Belén Cortés-Rodríguez, Juan Carlos Rodríguez-Aguilera, Sandra Rodríguez-López, Sarah J Mitchell, Kelsey N Murt, Krystle Kalafut, Katrina M Williams, Christopher W Ward, Joseph P Stains, Gloria Brea-Calvo, Jose M Villalba, Sonia Cortassa, Miguel A Aon, Rafael de Cabo

{"title":"Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice.","authors":"Michel Bernier, Ignacio Navas Enamorado, Mari Carmen Gómez-Cabrera, Miguel Calvo-Rubio, Jose Antonio González-Reyes, Nathan L Price, Ana Belén Cortés-Rodríguez, Juan Carlos Rodríguez-Aguilera, Sandra Rodríguez-López, Sarah J Mitchell, Kelsey N Murt, Krystle Kalafut, Katrina M Williams, Christopher W Ward, Joseph P Stains, Gloria Brea-Calvo, Jose M Villalba, Sonia Cortassa, Miguel A Aon, Rafael de Cabo","doi":"10.1038/s41514-022-00089-8","DOIUrl":"10.1038/s41514-022-00089-8","url":null,"abstract":"Skeletal muscle adapts to different exercise training modalities with age; however, the impact of both variables at the systemic and tissue levels is not fully understood. Here, adult and old C57BL/6 male mice were assigned to one of three groups: sedentary, daily high-intensity intermittent training (HIIT), or moderate intensity continuous training (MICT) for 4 weeks, compatible with the older group's exercise capacity. Improvements in body composition, fasting blood glucose, and muscle strength were mostly observed in the MICT old group, while effects of HIIT training in adult and old animals was less clear. Skeletal muscle exhibited structural and functional adaptations to exercise training, as revealed by electron microscopy, OXPHOS assays, respirometry, and muscle protein biomarkers. Transcriptomics analysis of gastrocnemius muscle combined with liver and serum metabolomics unveiled an age-dependent metabolic remodeling in response to exercise training. These results support a tailored exercise prescription approach aimed at improving health and ameliorating age-associated loss of muscle strength and function in the elderly.","PeriodicalId":19348,"journal":{"name":"npj Aging","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40670297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0