Nature biotechnology最新文献_第3页

Bioprinting 生物打印

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02662-4

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The Biotech Explorers Pathway equips students for STEM futures 生物技术探索者路径为学生的STEM未来做好准备

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02666-0

Anne E. Robinson, Barrie Cascella, Marta M. Wegorzewska, Daniel A. Berkovich, Joseph M. Jez

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Biopharma dives into tumor-seeking radioactive drugs 生物制药研究寻找肿瘤的放射性药物

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02681-1

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Engineering non-viral protein nanoparticles to deliver nucleic acids and proteins to cells 设计非病毒蛋白质纳米颗粒，将核酸和蛋白质输送到细胞

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02665-1

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Reprogramming endogenous regulatory DNA to fine-tune gene expression 重新编程内源性调控DNA以微调基因表达

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02686-w

Iris Marchal

{"title":"Reprogramming endogenous regulatory DNA to fine-tune gene expression","authors":"Iris Marchal","doi":"10.1038/s41587-025-02686-w","DOIUrl":"https://doi.org/10.1038/s41587-025-02686-w","url":null,"abstract":"Regulatory DNA sequences orchestrate cell-type-specific gene expression by facilitating transcription factor binding, yet their precise effects and reprogrammability remain challenging to delineate. Now, in Cell, Martyn et al. develop a method called variant effects from flow-sorting experiments with CRISPR targeting screens (Variant-EFFECTS), which measures the quantitative effects of changes to regulatory DNA on gene expression in endogenous contexts without the need for genetic engineering of reporters.Variant-EFFECTS uses pooled prime editing to introduce hundreds of noncoding edits to regulatory sequences in cells. The cells are then labeled with RNA FlowFISH or a fluorescent antibody targeted to a gene of interest, and sorted on the basis of levels of fluorescence. To demonstrate the usefulness of the method, the authors performed tiling mutagenesis screens targeting the promotor and/or enhancer regions for two genes involved in immune responses (PPIF and IL2RA) in THP-1 cells and Jurkat T cells. This revealed several motif instances with strong effects on gene-expression levels, including some missed by massively parallel reporter assays. Variant-EFFECTS can also dissect the context-specific effects of transcription factor motifs by inserting them across cell types. Moreover, the authors used Variant-EFFECTS data to benchmark deep-learning models that predict gene regulatory signals directly from DNA sequence, which revealed limitations to these models for predicting effect sizes of variants.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"27 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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People 人

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02670-4

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Brazil’s low-cost CAR-Ts take on Global South 巴西的低成本car - t向全球发展

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02691-z

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A gut-derived peptide protects citrus trees from Huanglongbing 一种来自肠道的肽可以保护柑橘树免受黄龙病的侵害

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02687-9

Iris Marchal

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3D bioprinting innovation and the patentability hurdle 3D生物打印创新和可专利性障碍

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-16 DOI: 10.1038/s41587-025-02661-5

Pratap Devarapalli, Dianne Nicol, Jane Nielsen

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Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins 自组装蛋白质纳米颗粒的细胞质输送核酸和蛋白质

IF 46.9 1区生物学

Nature biotechnology Pub Date : 2025-05-15 DOI: 10.1038/s41587-025-02664-2

Feyisayo Eweje, Vanessa Ibrahim, Aram Shajii, Michelle L. Walsh, Kiran Ahmad, Assma Alrefai, Dominie Miyasato, Jessie R. Davis, Hyunok Ham, Kaicheng Li, Michael Roehrl, Carolyn A. Haller, David R. Liu, Jiaxuan Chen, Elliot L. Chaikof

{"title":"Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins","authors":"Feyisayo Eweje, Vanessa Ibrahim, Aram Shajii, Michelle L. Walsh, Kiran Ahmad, Assma Alrefai, Dominie Miyasato, Jessie R. Davis, Hyunok Ham, Kaicheng Li, Michael Roehrl, Carolyn A. Haller, David R. Liu, Jiaxuan Chen, Elliot L. Chaikof","doi":"10.1038/s41587-025-02664-2","DOIUrl":"https://doi.org/10.1038/s41587-025-02664-2","url":null,"abstract":"Intracellular delivery of biomacromolecules is hampered by low efficiency and cytotoxicity. Here we report the development of elastin-based nanoparticles for therapeutic delivery (ENTER), a recombinant elastin-like polypeptide (ELP)-based delivery system for effective cytosolic delivery of biomacromolecules in vitro and in vivo. Through iterative design, we developed fourth-generation ELPs fused to cationic endosomal escape peptides (EEPs) that self-assemble into pH-responsive micellar nanoparticles and enable cytosolic entry of cargo following endocytic uptake. In silico screening of α-helical peptide libraries led to the discovery of an EEP (EEP13) with 48% improved protein delivery efficiency versus a benchmark peptide. Our lead ELP–EEP13 showed similar or superior performance compared to lipid-based transfection reagents in the delivery of mRNA-encoded, DNA-encoded and protein-form Cre recombinase and CRISPR gene editors as well as short interfering RNAs to multiple cell lines and primary cell types. Intranasal administration of ELP–EEP13 combined with Cre protein achieved efficient editing of lung epithelial cells in reporter mice.","PeriodicalId":19084,"journal":{"name":"Nature biotechnology","volume":"125 1","pages":""},"PeriodicalIF":46.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143979368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0