Molecular omics最新文献

{"title":"Analysis of N-glycosylation protein of Kashin–Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS/MS†","authors":"Sen Wang, Peilin Meng, Linlin Yuan and Xiong Guo","doi":"10.1039/D3MO00018D","DOIUrl":"https://doi.org/10.1039/D3MO00018D","url":null,"abstract":"We aimed to compare N-glycosylation proteins in Kashin-Beck disease (KBD) chondrocytes and normal chondrocytes derived from induced pluripotent stem cells (iPSCs). KBD and normal iPSCs were reprogrammed from human KBD and normal dermal fibroblasts, respectively. Subsequently, chondrocytes were differentiated from KBD and normal iPSCs separately. Immunofluorescence was utilized to assay the protein markers of iPSCs and chondrocytes. Differential N-glycosylation proteins were screened using label-free strategies with LC-MS/MS. Bioinformatics analyses were utilized to interpret the functions of differential N-glycosylation proteins. Immunofluorescence staining revealed that both KBD-iPSCs and normal-iPSCs strongly expressed pluripotency markers OCT4 and NANOG. Meanwhile, chondrocyte markers collagen II and SOX9 are presented in KBD-iPSC-chondrocytes and normal-iPSC-chondrocytes. We obtained 87 differential N-glycosylation sites which corresponded to 68 differential proteins, which were constructed into 1 cluster. We obtained collagen type I trimer and 9 other biological processes; polysaccharide binding and 9 other molecular functions; regulation of transcription by RNA polymerase II and 9 other cellular components from GO; the Pl3K-Akt signaling pathway and 9 other KEGG pathways; peroxisome and 7 other subcellular locations; and integrin alpha chain, C-terminal cytoplasmic region, conserved site and 9 other classifications of domain annotations, and 2 networks. FGFR3 and LRP1 are expressed at higher levels in KBD-iPSC-chondrocytes, while the expressions of COL2A1, TIMP1, UNC5B, NOG, LEPR, and ITGA1 were down-regulated in KBD-iPSC-chondrocytes. The differential expressions of these N-glycosylation proteins may lead to the abnormal function of KBD chondrocytes.","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 6","pages":" 454-463"},"PeriodicalIF":2.9,"publicationDate":"2023-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49995590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographical origin traceability of rice using a FTIR-based metabolomics approach†","authors":"Weifeng Xue, Qi Wang, Xuemei Li, Mei Wang, Zhenlin Dong, Haitao Bian and Fang Li","doi":"10.1039/D2MO00317A","DOIUrl":"10.1039/D2MO00317A","url":null,"abstract":"<p >Infrared spectroscopy is a crucial tool to achieve the origin traceability of rice, but it is constrained by data mining. In this study, a novel infrared spectroscopy-based metabolomics analytical method was proposed to discriminate rice products from 14 Chinese cities by seeking ‘wave number markers’. Principal component analysis (PCA), cluster analysis and orthogonal partial least squares discriminant analysis (OPLS-DA) were employed to separate all rice groups. The S-plot, permutation test and variable importance in projection (VIP) are used to screen eligible ‘markers’, which were further verified by a pairwise <em>t</em>-test. There are 55–265 ‘markers’ picked out from 14 rice groups, with their characteristic wave number bands to be 2935.658–3238.482, 3851.846–4000.364, 3329.136–3518.160, 1062.778–1213.225, 1161.147–1386.819, 3348.425–3560.594, 3115.038–3624.245, 2567.254–2872.007, 3334.923–3560.594, 3282.845–3543.235, 3338.780–3518.160, 3197.977–3560.594, 3163.258–3267.414 and 3292.489–3477.655 cm<small>⁻¹</small>, respectively. All but No. 5 rice groups show significantly low absorbance on their ‘marker’ bands. A mixed rice containing congenial No. 5 and No. 6 rice (80 : 20, <em>m</em>/<em>m</em>) was employed to test the validity of the method, and found that the ‘marker’ band of the mixed rice is the range of 1170.791–1338.598 cm<small>⁻¹</small>, implying the existence of considerable discrepancy between the mixed rice and other rice. The results indicate that infrared spectroscopy coupled with metabolomics analysis is competent for origin traceability of rice; thus, it provides a novel and workable approach for the accurate and rapid discrimination of rice from different geographical origins, and a distinctive perspective of metabolomics to explore infrared spectroscopy and beyond, especially not confined in the field of origin traceability.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 6","pages":" 504-513"},"PeriodicalIF":2.9,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of potential biomarkers for diagnosis of syphilis from the cerebrospinal fluid based on untargeted metabolomic analysis†","authors":"Liguo Liu, Dongmei Xu, Fengxin Chen, Shengnan Cai, Jin Wei, Jiaheng Deng, Jianhua Zheng, Qi Jin and Wenhui Lun","doi":"10.1039/D3MO00026E","DOIUrl":"10.1039/D3MO00026E","url":null,"abstract":"<p >The infection rate of syphilis continues to rise globally, and the difficulty in diagnosis of neurosyphilis promptly needs to be resolved. More specific and sensitive diagnostic markers for latent syphilis and neurosyphilis should be found. Here the metabolic profiles of 88 cerebrospinal fluid samples from syphilis patients and controls were analyzed by LC/MS-based untargeted metabolomics. In total, 272 metabolites based on 3937 features obtained in ESI− mode and 252 metabolites based on 3799 features in ESI+ mode were identified. The experimental process was evaluated by principal component analysis, partial least squares discriminant analysis, and hierarchical cluster analysis. A clear separation between latent syphilis and neurosyphilis was found. Levels of lipid and linoleic acid metabolites, such as 9-oxo-octadecadienoic acid and 9,10,13-trihydroxyoctadecenoic acid, were increased in syphilis patients. In patients with neurosyphilis, significant changes in levels of 5-hydroxy-<small>L</small>-tryptophan (5-HTP) and acetyl-<em>N</em>-formyl-5-methoxykynurenamine (AFMK) in the tryptophan–kynurenine pathway were also detected. Only one metabolite, theophylline, differed significantly between symptomatic and asymptomatic neurosyphilis patients. Additionally, KEGG analysis revealed significant enrichment of tryptophan metabolism pathways, indicating a high correlation between tryptophan metabolism and syphilis symptoms. Levels of linoleic acid metabolites, 5-HTP, AFMK and theophylline were significantly altered in different patients. The role of these differential metabolites in the development of syphilis is worthy of further exploration. Our results may promote the development of biomarkers for diagnosis of latent syphilis from neurosyphilis, and for that of asymptomatic neurosyphilis from symptomatic neurosyphilis in the future.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 6","pages":" 484-491"},"PeriodicalIF":2.9,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10151743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Metabolomics study reveals the alteration of fatty acid oxidation in the hearts of diabetic mice by empagliflozin","authors":"Yingwei Zhang, Zeyu Zhang, Chundi Li, Donge Tang and Yong Dai","doi":"10.1039/D3MO90012F","DOIUrl":"10.1039/D3MO90012F","url":null,"abstract":"<p >Correction for ‘Metabolomics study reveals the alteration of fatty acid oxidation in the hearts of diabetic mice by empagliflozin’ by Yingwei Zhang <em>et al.</em>, <em>Mol. Omics</em>, 2022, <strong>18</strong>, 643–651, https://doi.org/10.1039/D2MO00036A.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 4","pages":" 362-362"},"PeriodicalIF":2.9,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2023/mo/d3mo90012f?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9483598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What transcriptomics and proteomics can tell us about a high borate perturbed boron tolerant Bacilli strain†","authors":"Subhajit Sen, Sriradha Ganguli and Ranadhir Chakraborty","doi":"10.1039/D3MO00023K","DOIUrl":"10.1039/D3MO00023K","url":null,"abstract":"<p >A variety of genes work together to allow the bacterium <em>Lysinibacillus</em> sp. OL1 to survive and grow under B-stress circumstances. This bacterium was previously identified and described from agricultural soil treated with a boron fertilizer. The effects of B-stress on OL1 cells cultured in the presence of 200 mM boric acid were evaluated as changes in the log-phase cell transcriptome and proteome. OL1 has been found to upregulate all genes involved in producing critical macromolecules when exposed to B-stress. It was also observed that genes governing energy supply lines were in higher expression stages, indicating that they were more likely to support the increased production of macromolecules and stress-induced proteins, such as efflux proteins, to reduce boron damage and prevent boron accumulation inside the cell. It has been explained how the hub genes and bottleneck genes cooperate to survive boron stress and support bacterial growth. The proteome results have significantly confirmed the boron tolerance paradigm. Thus, the current study has improved our understanding of the bacterial B-stress response mechanism and opened new research directions.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 5","pages":" 370-382"},"PeriodicalIF":2.9,"publicationDate":"2023-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9666895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complete change in bile acids and steroids in systematic metabolomics applied to the intrahepatic cholestasis of pregnancy†","authors":"Hualin Xu, Yupin Xu, Guoqiang Zhao, Xukun Fu, Jian Zhao, Huaqian Wang, Yuliang Cai and Hongmei Lin","doi":"10.1039/D2MO00305H","DOIUrl":"10.1039/D2MO00305H","url":null,"abstract":"<p >Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific hepatobiliary disease, leading to an abnormal increase in total bile acid in the blood of pregnant women. To systematically explore the similarities and differences in metabolites and metabolic pathways among three types of biological samples from ICP women, a study of 18 ICP and 6 healthy (as a normal control) pregnant women was performed to investigate their clinical information and biochemical features. Based on validated LC–MS/MS methods 1–5 for hydrophilic and hydrophobic metabolites (molecular weight &lt;2000 Dalton), an untargeted-metabolomic strategy was applied to 24 pregnant women to determine the metabolites from 22 serum, 15 placental and 22 urine samples. Then 1137 metabolites from serum, 876 metabolites from placental tissue and 311 metabolites from urine with a coefficient of variation &lt;30% in the pooled quality control samples were found. Furthermore, orthogonal partial least squares–discriminate analysis (OPLS–DA), correlation analysis, chemical enrichment analysis and metabolic pathway analysis were carried out by a bioinformatics process. On the OPLS–DA model analysis, the metabolites in urine were better than those in serum or placental tissue to reflect the metabolic changes of ICP disease. Some metabolites were significantly changed in serum (<em>n</em> = 71), placental tissue (<em>n</em> = 46) and urine (<em>n</em> = 36), such as bile acids, triacylglycerols, lysoPCs, and steroids. Primary bile acid biosynthesis was the main metabolic pathway in ICP disease, and taurine and hypotaurine metabolism and sphingolipid metabolism were also found. More specifically, bile acids increased and steroids decreased in the serum, placental and urine samples. For complex metabolic diseases such as ICP disease, untargeted-metabolomic analysis of multiple biological samples could provide a systematic understanding of the changes in metabolic types and pathways.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 5","pages":" 418-428"},"PeriodicalIF":2.9,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2023/mo/d2mo00305h?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9720002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma metabolomic signatures from patients following high-dose total body irradiation†","authors":"Xiedong Hong, Lang Tian, Qiong Wu, Liming Gu, Wenli Wang, Hanxu Wu, Mingxiao Zhao, Xiaojin Wu and Chang Wang","doi":"10.1039/D2MO00274D","DOIUrl":"10.1039/D2MO00274D","url":null,"abstract":"<p >Despite some advances in the study of radiation injuries, effective methods of prevention and treatment of severe acute radiation syndrome or illness (ARS) are still lacking. Therefore, an in-depth understanding of the biological characteristics associated with high dose radiation is essential to reveal the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In the present study, plasma metabolic characteristics were investigated using hematopoietic stem cell transplantation patients (<em>n</em> = 36) undergoing total body ionizing irradiation (TBI) utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Plasma was collected pre-irradiation, 3 days after completion of fractionated radiation therapy with a total dose of 12 Gy delivered at a dose rate of 8 cGy min<small>⁻¹</small>. These metabolic disorders involve the dysregulation of the gut microflora, a shift in energy supply from aerobic respiration toward ketogenesis, protein synthesis and metabolism in response to TBI. Furthermore, the panel of four metabolic markers with most potential consisting of PC (O-38:5), urate, ornithine, and GCDCS for radiation injury was chosen by combining multiple methods of data processing that included univariate analysis, partial least squares discriminant analysis (PLS-DA), and multivariable stepwise linear regression analysis. While similar patterns of metabolic alterations were observed in patients of different genders, disease types and ages, specific changes were also found in specific patients following high doses of exposure. These findings provide valuable information for selecting metabolic biomarker panels for radiation injury, clues for radiation pathology and therapeutic interventions involved in high-dose radiation exposure.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 6","pages":" 492-503"},"PeriodicalIF":2.9,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10150112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-analyses of host metagenomes from colorectal cancer patients reveal strong relationship between colorectal cancer-associated species†","authors":"Manuel Adrian Riveros Escalona, Joice de Faria Poloni, Mathias J. Krause and Márcio Dorn","doi":"10.1039/D3MO00021D","DOIUrl":"10.1039/D3MO00021D","url":null,"abstract":"<p >Colorectal cancer (CRC) is one of the most common types of cancer, with many studies associating its development with changes in the gut microbiota. Recent developments in sequencing technologies and subsequent meta-analyses of gut metagenome provided a better understanding of species related to CRC tumorigenesis. Still, the importance of high-importance taxonomic singletons (<em>i.e.</em> species highly associated with a given condition but observed only in the minority of datasets) and the species interactions and co-occurrence across cohorts need further exploration. It has been shown that the gut metagenome presents a high functional redundancy, meaning that species interactions could mitigate the absence of any given species. In a CRC framework, this implies that species co-occurrence could play a role in tumorigenesis, even if CRC-associated species show low abundance. We propose to evaluate the prevalence of microbial species in tumor by initially analyzing each dataset individually and subsequently intersecting the results for differentially abundant species between CRC and healthy samples. We then identify metabolic pathways from these species based on KEGG orthologs, highlighting metabolic pathways associated with CRC. Our results indicate seven species with high prevalence across all projects and with high association to CRC, including the genus <em>Bacteroides</em>, <em>Enterocloster</em> and <em>Prevotella</em>. Finally, we show that CRC is also characterized by the co-occurrence of species that do not present significant differential abundance, but have been described in the literature as potential CRC biomarkers. These results indicate that between-species interactions could also play a role in CRC tumorigenesis.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 5","pages":" 429-444"},"PeriodicalIF":2.9,"publicationDate":"2023-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9720464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated proteomic and metabolomic profiling of urine of renal anemia patients uncovers the molecular mechanisms of roxadustat†","authors":"Xiaoe You, Baochun Guo, Zhen Wang, Hualin Ma, Lixia Liu, Ru Zhou, Yaxuan Zheng and Xinzhou Zhang","doi":"10.1039/D3MO00015J","DOIUrl":"10.1039/D3MO00015J","url":null,"abstract":"<p >Roxadustat (FG-4592) is a hypoxia-inducible factor prolyl hydroxylase inhibitor (HIF-PHI) prescribed to patients with low hemoglobin associated with chronic kidney disease. Due to the various HIF-mediated adaptive responses, FG-4592 has attracted significant interest for therapeutic use against various diseases. However, the clinical application of Roxadustat remains limited due to a lack of understanding of its underlying mechanisms. Herein, we performed label-free quantitative liquid chromatography with tandem mass spectrometry (LC-MS–MS) proteomics and un-targeted metabolomics to study the protein and metabolite alterations in the urine of renal anemia patients before and after Roxadustat therapy. The results were validated by parallel reaction monitoring (PRM). A total of 46 proteins (including 15 upregulated and 31 downregulated proteins) and 207 metabolites were significantly altered after Roxadustat treatment in urine samples obtained from renal anemia patients. Then, the altered proteins were further validated by PRM. Finally, proteomics combined with metabolomics analysis revealed that the Ras signalling pathway, cysteine and methionine metabolism, arginine and proline metabolism, and cholesterol metabolism were the main pathways altered by Roxadustat treatment. The multi-omics analysis revealed that Roxadustat could alter the protein expression and reverse the potential metabolic changes to exert hypotensive, lipid metabolic regulation, and renoprotective effects in clinical practice.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 6","pages":" 473-483"},"PeriodicalIF":2.9,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2023/mo/d3mo00015j?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9781007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique†","authors":"April Rees, Zoe Edwards-I-Coll, Oliver Richards, Molly E Raikes, Roberto Angelini and Catherine A Thornton","doi":"10.1039/D2MO00294A","DOIUrl":"10.1039/D2MO00294A","url":null,"abstract":"<p >The lipid environment changes throughout pregnancy both physiologically with emergent insulin resistance and pathologically <em>e.g.</em>, gestational diabetes mellitus (GDM). Novel mass spectrometry (MS) techniques applied to minimally processed blood might lend themselves to monitoring changing lipid profiles to inform care decisions across pregnancy. In this study we use an intact-sandwich, MALDI-ToF MS method to identify phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) species and calculate their ratio as an indicator of inflammation. Plasma and sera were prepared from venous blood of non-pregnant women (aged 18–40) and pregnant women at 16 weeks, 28 weeks (including GDM-positive women), and 37+ weeks (term) of gestation alongside umbilical cord blood (UCB). Women with a normal menstrual cycle and age-matched men provided finger-prick derived capillary sera at 6 time-points over a month. Serum rather than plasma was preferable for PC/LPC measurement. As pregnancy progresses, an anti-inflammatory phenotype dominates the maternal circulation, evidenced by increasing PC/LPC ratio. In contrast, the PC/LPC ratio of UCB was aligned to that of non-pregnant donors. BMI had no significant effect on the PC/LPC ratio, but GDM-complicated pregnancies had significantly lower PC/LPC at 16 weeks of gestation. To further translate the use of the PC/LPC ratio clinically, the utility of finger-prick blood was evaluated; no significant difference between capillary <em>versus</em> venous serum was found and we revealed the PC/LPC ratio oscillates with the menstrual cycle. Overall, we show that the PC/LPC ratio can be measured simply in human serum and has the potential to be used as a time-efficient and less invasive biomarker of (mal)adaptative inflammation.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 4","pages":" 340-350"},"PeriodicalIF":2.9,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2023/mo/d2mo00294a?page=search","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9440542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}