Nanomedicine : nanotechnology, biology, and medicine最新文献_第6页

Quercetin conjugated PSC-derived exosomes to inhibit intimal hyperplasia via modulating the ERK, Akt, and NF-κB signaling pathways in the rat carotid artery post balloon injury 槲皮素共轭 PSC 衍生外泌体通过调节球囊损伤后大鼠颈动脉中的 ERK、Akt 和 NF-κB 信号通路抑制内膜增生。

IF 4.2 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-17 DOI: 10.1016/j.nano.2024.102763

Xin Mao MD , Yaming Du MD , Rubo Sui MD , Xiaodong Yu MD , Yue Zhu MD , Meiyi Huang MD

{"title":"Quercetin conjugated PSC-derived exosomes to inhibit intimal hyperplasia via modulating the ERK, Akt, and NF-κB signaling pathways in the rat carotid artery post balloon injury","authors":"Xin Mao MD , Yaming Du MD , Rubo Sui MD , Xiaodong Yu MD , Yue Zhu MD , Meiyi Huang MD","doi":"10.1016/j.nano.2024.102763","DOIUrl":"10.1016/j.nano.2024.102763","url":null,"abstract":"<div><p>The primary challenge in percutaneous coronary interventions for vascular restenosis is the occurrence of restenosis, which is defined by the excessive proliferation of neointimal tissue. Herein, our research team suggests that exosomes obtained from PSC, when paired with quercetin (Q@PSC-E), successfully reduce neointimal hyperplasia in a Sprague-Dawley rat model. Furthermore, the physical properties of the synthesized Q@PSC-E were examined using UV–vis, DLS, and FT-IR characterization techniques. The rats were subjected to balloon injury (BI) utilizing a 2-Fr Fogarty arterial embolectomy balloon catheter. Intimal hyperplasia and the degree of VSMC proliferation were evaluated using histological analysis in the rat groups that received a dosage of Q@PSC-E at 30 mg/kg/d. Significantly, Q@PSC-E inhibited cell proliferation through a pathway that does not include lipoxygenase, as demonstrated by [<sup>3</sup>H] thymidine incorporation, MTT, and flow cytometry studies. Additionally, the data indicate that Q@PSC-E hinders cell proliferation by targeting particular events that promote cell growth, including the activation of Akt and NF-κB, disruption of cell-cycle progression and also obstructs the ERK signaling pathway.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"61 ","pages":"Article 102763"},"PeriodicalIF":4.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MoO3-X nanodots coated suture for combating surgical site infection via antibacterial and anti-inflammatory properties MoO3-X 纳米点涂层缝合线通过抗菌和消炎特性防止手术部位感染。

IF 4.2 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-16 DOI: 10.1016/j.nano.2024.102757

Jingyu Zhang MSc , Xuexiao Li MSc , Ming Cheng MD , Kaichen Wan MSc , Shangcheng Yan MSc , Wei Peng MSc , Guangxin Duan MD , Yongyou Wu MD , Ling Wen MD

{"title":"MoO3-X nanodots coated suture for combating surgical site infection via antibacterial and anti-inflammatory properties","authors":"Jingyu Zhang MSc , Xuexiao Li MSc , Ming Cheng MD , Kaichen Wan MSc , Shangcheng Yan MSc , Wei Peng MSc , Guangxin Duan MD , Yongyou Wu MD , Ling Wen MD","doi":"10.1016/j.nano.2024.102757","DOIUrl":"10.1016/j.nano.2024.102757","url":null,"abstract":"<div><p>Surgical site infection (SSI) significantly affects patient recovery time, health outcomes and quality of life which is closely associated with the use of implants or mesh. Sutures are the most frequently used implants that play a significant role in the development of SSI. Studies have demonstrated that the administration of effective bactericidal and anti-inflammatory treatments can significantly decrease the incidence of SSI. To address this concern, a versatile suture was engineered by coating MoO<sub>3-X</sub> nanodots in this study. The incorporation of MoO<sub>3-X</sub> nanodots endowed the suture with desirable antibacterial and anti-inflammatory properties that were evaluated in <em>in vitro</em> and <em>in vivo</em> experiments. The results showed its remarkable ability to facilitate wound healing and prevent SSI through its dual action of combating bacterial infection and reducing inflammation. These findings highlight the promising potential of this multifunctional surgical suture as a versatile tool to promote better outcomes in surgical procedures.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102757"},"PeriodicalIF":4.2,"publicationDate":"2024-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141420002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blocking the utilization of carbon sources via two pathways to induce tumor starvation for cancer treatment 通过两种途径阻断碳源利用，诱导肿瘤饥饿以治疗癌症

IF 4.2 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-15 DOI: 10.1016/j.nano.2024.102764

Zhihui Zhu MSc , Pan Qiao MSc , Mengyu Liu MSc , Fangfang Sun MSc , Meilin Geng MSc , Hanchun Yao PhD

{"title":"Blocking the utilization of carbon sources via two pathways to induce tumor starvation for cancer treatment","authors":"Zhihui Zhu MSc , Pan Qiao MSc , Mengyu Liu MSc , Fangfang Sun MSc , Meilin Geng MSc , Hanchun Yao PhD","doi":"10.1016/j.nano.2024.102764","DOIUrl":"10.1016/j.nano.2024.102764","url":null,"abstract":"<div><p>Glucose oxidase (GOx) is often used to starvation therapy. However, only consuming glucose cannot completely block the energy metabolism of tumor cells. Lactate can support tumor cell survival in the absence of glucose. Here, we constructed a nanoplatform (Met@HMnO<sub>2</sub>-GOx/HA) that can deplete glucose while inhibiting the compensatory use of lactate by cells to enhance the effect of tumor starvation therapy. GOx can catalyze glucose into gluconic acid and H<sub>2</sub>O<sub>2</sub>, and then HMnO<sub>2</sub> catalyzes H<sub>2</sub>O<sub>2</sub> into O<sub>2</sub> to compensate for the oxygen consumed by GOx, allowing the reaction to proceed sustainably. Furthermore, metformin (Met) can inhibit the conversion of lactate to pyruvate in a redox-dependent manner and reduce the utilization of lactate by tumor cells. Met@HMnO<sub>2</sub>-GOx/HA nanoparticles maximize the efficacy of tumor starvation therapy by simultaneously inhibiting cellular utilization of two carbon sources. Therefore, this platform is expected to provide new strategies for tumor treatment.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"61 ","pages":"Article 102764"},"PeriodicalIF":4.2,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141398167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted-delivery of nanomedicine-enabled methylprednisolone to injured spinal cord promotes neuroprotection and functional recovery after acute spinal cord injury in rats 在大鼠急性脊髓损伤后，向损伤脊髓靶向投放纳米药物甲基强的松龙可促进神经保护和功能恢复。

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-11 DOI: 10.1016/j.nano.2024.102761

Wei Zhao PhD , Zhenshan Jia PhD , William A. Bauman MD , Yiwen Qin BA , Yuanzhen Peng BA , Zihao Chen MS , Christopher P. Cardozo MD , Dong Wang PhD , Weiping Qin MD, Ph.D

{"title":"Targeted-delivery of nanomedicine-enabled methylprednisolone to injured spinal cord promotes neuroprotection and functional recovery after acute spinal cord injury in rats","authors":"Wei Zhao PhD , Zhenshan Jia PhD , William A. Bauman MD , Yiwen Qin BA , Yuanzhen Peng BA , Zihao Chen MS , Christopher P. Cardozo MD , Dong Wang PhD , Weiping Qin MD, Ph.D","doi":"10.1016/j.nano.2024.102761","DOIUrl":"10.1016/j.nano.2024.102761","url":null,"abstract":"<div><p>To date, no therapy has been proven to be efficacious in fully restoring neurological functions after spinal cord injury (SCI). Systemic high-dose methylprednisolone (MP) improves neurological recovery after acute SCI in both animal and human. MP therapy remains controversial due to its modest effect on functional recovery and significant adverse effects. To overcome the limitation of MP therapy, we have developed a <em>N</em>-(2-hydroxypropyl) methacrylamide copolymer-based MP prodrug nanomedicine (Nano-MP) that can selectively deliver MP to the SCI lesion when administered systemically in a rat model of acute SCI. Our in vivo data reveal that Nano-MP is significantly more effective than free MP in attenuating secondary injuries and neuronal apoptosis. Nano-MP is superior to free MP in improving functional recovery after acute SCI in rats. These data support Nano-MP as a promising neurotherapeutic candidate, which may provide potent neuroprotection and accelerate functional recovery with improved safety for patients with acute SCI.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102761"},"PeriodicalIF":5.4,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141317769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunostimulatory nucleic acid nanoparticles (NANPs) augment protective osteoblast and osteoclast type I interferon responses to Staphylococcus aureus 免疫刺激核酸纳米颗粒（NANPs）可增强保护性成骨细胞和破骨细胞对金黄色葡萄球菌的 I 型干扰素反应。

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-10 DOI: 10.1016/j.nano.2024.102762

Erin L. Mills PhD , Yelixza I. Avila PhD , Damian Beasock PhD , Yasmine Radwan PhD , Samantha R. Suptela PhD , Ian Marriott PhD , Kirill A. Afonin PhD , M. Brittany Johnson PhD

{"title":"Immunostimulatory nucleic acid nanoparticles (NANPs) augment protective osteoblast and osteoclast type I interferon responses to Staphylococcus aureus","authors":"Erin L. Mills PhD , Yelixza I. Avila PhD , Damian Beasock PhD , Yasmine Radwan PhD , Samantha R. Suptela PhD , Ian Marriott PhD , Kirill A. Afonin PhD , M. Brittany Johnson PhD","doi":"10.1016/j.nano.2024.102762","DOIUrl":"10.1016/j.nano.2024.102762","url":null,"abstract":"<div><p>Recalcitrant staphylococcal osteomyelitis may be due, in part, to the ability of <em>Staphylococcus aureus</em> to invade bone cells. However, osteoclasts and osteoblasts are now recognized to shape host responses to bacterial infection and we have recently described their ability to produce IFN-β following <em>S. aureus</em> infection and limit intracellular bacterial survival/propagation. Here, we have investigated the ability of novel, rationally designed, nucleic acid nanoparticles (NANPs) to induce the production of immune mediators, including IFN-β, following introduction into bone cells. We demonstrate the successful delivery of representative NANPs into osteoblasts and osteoclasts via endosomal trafficking when complexed with lipid-based carriers. Their delivery was found to differentially induce immune responses according to their composition and architecture via discrete cytosolic pattern recognition receptors. Finally, the utility of this nanoparticle technology was supported by the demonstration that immunostimulatory NANPs augment IFN-β production by <em>S. aureus</em> infected bone cells and reduce intracellular bacterial burden.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102762"},"PeriodicalIF":5.4,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141311164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of TNF-α with specific isoelectric point released from SPIONs basing on variable charge to enhance pH-sensitive controlled-release 根据可变电荷重构从 SPION 释放的具有特定等电点的 TNF-α，以提高 pH 值敏感性控制释放。

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-07 DOI: 10.1016/j.nano.2024.102758

Lin Yan Ph.D , Yadi Chen M.Sc , Shihao Zhang M.Sc , Chunjie Zhu Ph.D , Shangying Xiao M.Sc , Haishan Xia M.Sc , Xiaohua Chen M.Sc , Dan Guo Ph.D , Xiaohua Lv M.Sc , Lei Rao Ph.D , Manjiao Zhuang Ph.D

{"title":"Reconstruction of TNF-α with specific isoelectric point released from SPIONs basing on variable charge to enhance pH-sensitive controlled-release","authors":"Lin Yan Ph.D , Yadi Chen M.Sc , Shihao Zhang M.Sc , Chunjie Zhu Ph.D , Shangying Xiao M.Sc , Haishan Xia M.Sc , Xiaohua Chen M.Sc , Dan Guo Ph.D , Xiaohua Lv M.Sc , Lei Rao Ph.D , Manjiao Zhuang Ph.D","doi":"10.1016/j.nano.2024.102758","DOIUrl":"10.1016/j.nano.2024.102758","url":null,"abstract":"<div><p>The clinical application of tumor necrosis factor-α (TNF-α) is limited by its short half-life, subeffective concentration in the targeted area and severe systemic toxicity. In this study, the recombinant polypeptide S4-TNF-α was constructed and coupled with chitosan-modified superparamagnetic iron oxide nanoparticles (S4-TNF-α-SPIONs) to achieve pH-sensitive controlled release and active tumor targeting activity. The isoelectric point (pI) of S4-TNF-α was reconstructed to approach the pH of the tumor microenvironment. The negative-charge S4-TNF-α was adsorbed to chitosan-modified superparamagnetic iron oxide nanoparticles (CS-SPIONs) with a positive charge through electrostatic adsorption at physiological pH. The acidic tumor microenvironment endowed S4-TNF-α with a zero charge, which accelerated S4-TNF-α release from CS-SPIONs. Our studies showed that S4-TNF-α-SPIONs displayed an ideal pH-sensitive controlled release capacity and improved antitumor effects. Our study presents a novel approach to enhance the pH-sensitive controlled-release of genetically engineered drugs by adjusting their pI to match the pH of the tumor microenvironment.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102758"},"PeriodicalIF":5.4,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of red blood cell-derived extracellular particles as a biocompatible nanocarrier of microRNA-204 (REP-204) to harness anti-neuroblastoma effect 开发源自红细胞的细胞外颗粒，作为 microRNA-204 (REP-204) 的生物相容性纳米载体，以发挥抗神经母细胞瘤的作用。

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-07 DOI: 10.1016/j.nano.2024.102760

Wararat Chiangjong Ph.D. , Jirawan Panachan M.S. , Sujitra Keadsanti Ph.D. , David S. Newburg Ph.D. , Ardythe L. Morrow Ph.D. , Suradej Hongeng M.D. , Somchai Chutipongtanate M.D., Ph.D.

{"title":"Development of red blood cell-derived extracellular particles as a biocompatible nanocarrier of microRNA-204 (REP-204) to harness anti-neuroblastoma effect","authors":"Wararat Chiangjong Ph.D. , Jirawan Panachan M.S. , Sujitra Keadsanti Ph.D. , David S. Newburg Ph.D. , Ardythe L. Morrow Ph.D. , Suradej Hongeng M.D. , Somchai Chutipongtanate M.D., Ph.D.","doi":"10.1016/j.nano.2024.102760","DOIUrl":"10.1016/j.nano.2024.102760","url":null,"abstract":"<div><p>Neuroblastoma (NB) is the most common extracranial solid tumor in the pediatric population with a high degree of heterogeneity in clinical outcomes. Upregulation of the tumor suppressor miR-204 in neuroblastoma is associated with good prognosis. Although miR-204 has been recognized as a potential therapeutic candidate, its delivery is unavailable. We hypothesized that REP-204, the red blood cell-derived extracellular particles (REP) with miR-204 loading, can suppress neuroblastoma cells in vitro. After miR-204 loading by electroporation, REP-204, but not REP carriers, inhibited the viability, migration, and 3D spheroid growth of neuroblastoma cells regardless of MYCN amplification status. SWATH-proteomics revealed that REP-204 treatment may trigger a negative regulation of mRNA splicing by the spliceosome, suppression of amino acid metabolism and protein production, and prevent SLIT/ROBO signaling-mediated cell migration, to halt neuroblastoma tumor growth and metastasis. The therapeutic efficacy of REP-204 should be further investigated in preclinical models and clinical studies.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102760"},"PeriodicalIF":5.4,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1549963424000297/pdfft?md5=ef23c07d03b9cd57361c33044a34dce6&pid=1-s2.0-S1549963424000297-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of capsaicin and calcium phosphate-loaded MOF system for tumor therapy involving calcium overload 将辣椒素和磷酸钙负载的 MOF 系统应用于涉及钙超载的肿瘤治疗。

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-06 DOI: 10.1016/j.nano.2024.102759

Yuan Gao MSc , Jun Wang PhD , Weiwei Zhang PhD , Fei Ge PhD , Wanzhen Li PhD , Feiyang Xu MSc , Ting Cui MSc , Xing Li MSc , Kai Yang PhD , Yugui Tao MSc

{"title":"Application of capsaicin and calcium phosphate-loaded MOF system for tumor therapy involving calcium overload","authors":"Yuan Gao MSc , Jun Wang PhD , Weiwei Zhang PhD , Fei Ge PhD , Wanzhen Li PhD , Feiyang Xu MSc , Ting Cui MSc , Xing Li MSc , Kai Yang PhD , Yugui Tao MSc","doi":"10.1016/j.nano.2024.102759","DOIUrl":"10.1016/j.nano.2024.102759","url":null,"abstract":"<div><p>Calcium overload therapy refers to the condition of intracellular Ca<sup>2+</sup> overload, which causes mitochondrial damage and leads to the uncontrolled release of apoptotic factors into the cytoplasm through the open mitochondrial permeability pore. Based on this, it is playing an increasingly important role in the field of oncology due to its good efficacy and small side effects. However, the regulation of calcium homeostasis by cancer cells themselves, insufficient calcium ions (Ca<sup>2+</sup>) in tumor sites and low efficiency of calcium entering tumor have limited its efficacy, resulting in unsatisfactory therapeutic effect. Therefore, a novel CAP/BSA@TCP-ZIF-8 nanoparticle drug carrier system was constructed that can provide Ca<sup>2+</sup> from exogenous sources for pH-controlled degradation and drug release at the same time. Both <em>in vivo</em> and <em>in vitro</em> experiments have proved that the nanomaterial can activate TRPV1 channels and provide exogenous Ca<sup>2+</sup> to cause Ca<sup>2+</sup> overload and apoptosis, thus achieving anti-tumor effects.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102759"},"PeriodicalIF":5.4,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge collagen biocomposite reinforced with 2D nano-talc for bone tissue engineering 用于骨组织工程的尖端胶原蛋白生物复合材料，采用二维纳米钙增强。

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-06-06 DOI: 10.1016/j.nano.2024.102756

Ana Carolina Ferreira de Brito PhD , Samuel Marques de Sousa BSc , Helane Lucia Oliveira de Morais MSc , Pedro Henrique Mendes da Costa BSc , Nathanael Vieira Medrado MSc , Mariana de Castro Prado PhD , Ingrid David Barcelos PhD , Érika Costa de Alvarenga PhD , Bernardo Ruegger Almeida Neves PhD , Ana Paula Moreira Barboza PhD , Taíse Matte Manhabosco PhD

{"title":"Cutting-edge collagen biocomposite reinforced with 2D nano-talc for bone tissue engineering","authors":"Ana Carolina Ferreira de Brito PhD , Samuel Marques de Sousa BSc , Helane Lucia Oliveira de Morais MSc , Pedro Henrique Mendes da Costa BSc , Nathanael Vieira Medrado MSc , Mariana de Castro Prado PhD , Ingrid David Barcelos PhD , Érika Costa de Alvarenga PhD , Bernardo Ruegger Almeida Neves PhD , Ana Paula Moreira Barboza PhD , Taíse Matte Manhabosco PhD","doi":"10.1016/j.nano.2024.102756","DOIUrl":"10.1016/j.nano.2024.102756","url":null,"abstract":"<div><p>The advancement of nanobiocomposites reinforced with 2D nano-materials plays a pivotal role in enhancing bone tissue engineering. In this study, we introduce a nanobiocomposite that reinforces bovine collagen with <em>2D nano</em>-talc, a recently exfoliated nano-mineral. These nanobiocomposites were prepared by blending collagen with varying concentrations of <em>2D nano</em>-talc, encompassing mono- and few-layers talc from soapstone nanomaterial. Extensive characterization techniques including AFM, XPS, nano-FTIR, s-SNOM nanoimaging, Force Spectroscopy, and PeakForce QNM® were employed. The incorporation of <em>2D nano</em>-talc significantly enhanced the mechanical properties of the nanobiocomposites, resulting in increased stiffness compared to pristine collagen. <em>In vitro</em> studies supported the growth and proliferation of osteoblasts onto <em>2D nano</em>-talc-reinforced nanobiocomposites, as well as showed the highest mineralization potential. These findings highlight the substantial potential of the developed nanobiocomposite as a <em>scaffold</em> material for bone tissue engineering applications.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"60 ","pages":"Article 102756"},"PeriodicalIF":5.4,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of ESCCAL-1 in regulating exocytosis of AuNPs in human esophageal squamous carcinoma cells ESCCAL-1 在调节人食管鳞癌细胞 AuNPs 外吞过程中的作用

IF 5.4 2区医学

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2024-05-24 DOI: 10.1016/j.nano.2024.102754

Fenfen Gong MSc , Yuanbo Cui PhD , Pengju Lv PhD , Jia Liu MSc , Xiaoyan Sun MSc , Pengli Han MSc , Lijuan Zhou MSc , Tian Xia PhD , Wei Cao PhD

{"title":"Role of ESCCAL-1 in regulating exocytosis of AuNPs in human esophageal squamous carcinoma cells","authors":"Fenfen Gong MSc , Yuanbo Cui PhD , Pengju Lv PhD , Jia Liu MSc , Xiaoyan Sun MSc , Pengli Han MSc , Lijuan Zhou MSc , Tian Xia PhD , Wei Cao PhD","doi":"10.1016/j.nano.2024.102754","DOIUrl":"10.1016/j.nano.2024.102754","url":null,"abstract":"<div><p>Exocytosis is a critical factor for designing efficient nanocarriers and determining cytotoxicity. However, the research on the exocytosis mechanism of nanoparticles, especially the role of long non-coding RNAs (lncRNAs), has not been reported. In this study, the exocytosis of AuNPs in the KYSE70 cells and the involved molecular pathways of exocytosis are analyzed. It demonstrates that nanoparticles underwent time-dependent release from the cells by exocytosis, and the release of β-hexosaminidase confirms that AuNPs are excreted through lysosomes. Mechanistic studies reveal that lncRNA ESCCAL-1 plays a vital role in controlling the exocytosis of AuNPs through activation of the MAPK pathway, including the phosphorylation of ERK and JNK. The study implies that the ESCCAL-1-mediated pathway plays an important role in the exocytosis of AuNPs in KYSE70 cells. This finding has implications for the role of ESCCAL-1 on the drug resistance of esophagus cancer by controlling lysosome-mediated exocytosis.</p></div>","PeriodicalId":19050,"journal":{"name":"Nanomedicine : nanotechnology, biology, and medicine","volume":"59 ","pages":"Article 102754"},"PeriodicalIF":5.4,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141141031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0