Mycological research最新文献

A beauvericin hot spot in the genus Isaria 伊撒利亚属beauvericin的热点

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.08.017

J. Jennifer Luangsa-ard , Pitchapa Berkaew , Rungpet Ridkaew , Nigel L. Hywel-Jones , Masahiko Isaka

{"title":"A beauvericin hot spot in the genus Isaria","authors":"J. Jennifer Luangsa-ard , Pitchapa Berkaew , Rungpet Ridkaew , Nigel L. Hywel-Jones , Masahiko Isaka","doi":"10.1016/j.mycres.2009.08.017","DOIUrl":"10.1016/j.mycres.2009.08.017","url":null,"abstract":"<div>Beauvericin is a naturally occurring cyclohexadepsipeptide originally described from Beauveria bassiana but also reported from several Fusarium species as well as members of the genus Isaria. Twenty-six isolates of Isaria species and its Cordyceps teleomorph, and ten taxonomically close strains including Beauveria, Nomuraea and Paecilomyces species were sequenced and tested for beauvericin production. Trees using ITS rDNA and β-tubulin sequence data were constructed and used to infer the phylogenetic distribution of beauvericin production. A group comprising Isaria tenuipes and its known teleomorph Cordyceps takaomontana, Isaria cicadae and its Cordyceps teleomorph, Isaria japonica and Isaria fumosorosea, showed positive beauvericin production which correlated well with combined ITS rDNA and β-tubulin phylogenies. The results suggested that beauvericin can serve as a chemotaxonomic marker for these limited species of the I. tenuipes complex.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.08.017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28413361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Dynamics of energy charge and adenine nucleotides during uncoupling of catabolism and anabolism in Penicillium ochrochloron 绿绿青霉分解代谢和合成代谢解偶联过程中能量、电荷和腺嘌呤核苷酸的动力学

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.09.011

Pamela Vrabl, Wolfgang Mutschlechner , Wolfgang Burgstaller

{"title":"Dynamics of energy charge and adenine nucleotides during uncoupling of catabolism and anabolism in Penicillium ochrochloron","authors":"Pamela Vrabl, Wolfgang Mutschlechner , Wolfgang Burgstaller","doi":"10.1016/j.mycres.2009.09.011","DOIUrl":"10.1016/j.mycres.2009.09.011","url":null,"abstract":"<div>Filamentous fungi are able to spill energy when exposed to energy excess by uncoupling catabolism from anabolism, e.g. via overflow metabolism. In current study we tested the hypothesis that overflow metabolism is regulated via the energetic status of the hyphae (i.e. energy charge, ATP concentration). This hypothesis was studied in Penicillium ochrochloron during the steady state of glucose- or ammonium-limited chemostat cultures as well as during three transient states ((i) glucose pulse to a glucose-limited chemostat, (ii) shift from glucose-limited to ammonium-limited conditions in a chemostat, and (iii) ammonium exhaustion in batch culture). Organic acids were excreted under all conditions, even during exponential growth in batch culture as well as under glucose-limited conditions in a chemostat. Partial uncoupling of catabolism and anabolism via overflow metabolism was thus constitutively present. Under all tested conditions, overflow metabolism was independent of the energy charge or the ATP concentration of the hyphae. There was a reciprocal correlation between glucose uptake rate and intracellular adenine nucleotide content. During all transients states a rapid decrease in energy charge and the concentrations of nucleotides was observed shortly after a change in glycolytic flux (“ATP paradoxon”). A possible connection between the change in adenine nucleotide concentrations and the purine salvage pathway is discussed.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.09.011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28428919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Book Reviews☆ 书评☆

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.11.002

引用次数: 0

Trichoderma species form endophytic associations within Theobroma cacao trichomes 木霉在可可树的毛状体中形成内生结合力

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.09.004

Bryan A. Bailey , Mary D. Strem , Delilah Wood

{"title":"Trichoderma species form endophytic associations within Theobroma cacao trichomes","authors":"Bryan A. Bailey , Mary D. Strem , Delilah Wood","doi":"10.1016/j.mycres.2009.09.004","DOIUrl":"10.1016/j.mycres.2009.09.004","url":null,"abstract":"<div>Trichoderma species are usually considered soil organisms that colonize plant roots, sometimes forming a symbiotic relationship. Recent studies demonstrate that Trichoderma species are also capable of colonizing the above ground tissues of Theobroma cacao (cacao) in what has been characterized as an endophytic relationship. Trichoderma species can be re-isolated from surface sterilized cacao stem tissue, including the bark and xylem, the apical meristem, and to a lesser degree from leaves. SEM analysis of cacao stems colonized by strains of four Trichoderma species (Trichoderma ovalisporum-DIS 70a, Trichoderma hamatum-DIS 219b, Trichoderma koningiopsis-DIS 172ai, or Trichoderma harzianum-DIS 219f) showed a preference for surface colonization of glandular trichomes versus non-glandular trichomes. The Trichoderma strains colonized the glandular trichome tips and formed swellings resembling appresoria. Hyphae were observed emerging from the glandular trichomes on surface sterilized stems from cacao seedlings that had been inoculated with each of the four Trichoderma strains. Fungal hyphae were observed under the microscope emerging from the trichomes as soon as 6h after their isolation from surface sterilized cacao seedling stems. Hyphae were also observed, in some cases, emerging from stalk cells opposite the trichome head. Repeated single trichome/hyphae isolations verified that the emerging hyphae were the Trichoderma strains with which the cacao seedlings had been inoculated. Strains of four Trichoderma species were able to enter glandular trichomes during the colonization of cacao stems where they survived surface sterilization and could be re-isolated. The penetration of cacao trichomes may provide the entry point for Trichoderma species into the cacao stem allowing systemic colonization of this tissue.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.09.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28411762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 75

Molecular and ultrastructural characterization of two ascomycetes found on sunken wood off Vanuatu Islands in the deep Pacific Ocean 太平洋深处瓦努阿图群岛沉没木材上发现的两种子囊菌的分子和超微结构特征

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.08.015

Joëlle Dupont , Sandrine Magnin , Florence Rousseau , Magali Zbinden , Ghislaine Frebourg , Sarah Samadi , Bertrand Richer de Forges , E.B. Gareth Jones

引用次数: 39

Antifungal metabolites (monorden, monocillins I, II, III) from Colletotrichum graminicola, a systemic vascular pathogen of maize 玉米系统性维管病原菌graminicola的抗真菌代谢产物(monorden, monocillins I, II, III)

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.10.001

Donald T. Wicklow , Annalisa M. Jordan , James B. Gloer

{"title":"Antifungal metabolites (monorden, monocillins I, II, III) from Colletotrichum graminicola, a systemic vascular pathogen of maize","authors":"Donald T. Wicklow , Annalisa M. Jordan , James B. Gloer","doi":"10.1016/j.mycres.2009.10.001","DOIUrl":"10.1016/j.mycres.2009.10.001","url":null,"abstract":"<div>Colletotrichum graminicola is a systemic vascular pathogen that causes anthracnose stalk rot and leaf blight of maize. In the course of an effort to explore the potential presence and roles of C. graminicola metabolites in maize, ethyl acetate extracts of solid substrate fermentations of several C. graminicola isolates from Michigan and Illinois were found to be active against Aspergillus flavus and Fusarium verticillioides, both mycotoxin-producing seed-infecting fungal pathogens. Chemical investigations of the extract of one such isolate (NRRL 47511) led to the isolation of known metabolites monorden (also known as radicicol) and monocillins I–III as major components. Monorden and monocillin I displayed in vitro activity against the stalk- and ear-rot pathogen Stenocarpella maydis while only the most abundant metabolite (monorden) showed activity against foliar pathogens Alternaria alternata, Bipolaris zeicola, and Curvularia lunata. Using LC–HRESITOFMS, monorden was detected in steam-sterilized maize stalks and stalk residues inoculated with C. graminicola but not in the necrotic stalk tissues of wound-inoculated plants grown in an environmental chamber. Monorden and monocillin I can bind and inhibit plant Hsp90, a chaperone of R-proteins. It is hypothesized that monorden and monocillins could support the C. graminicola disease cycle by disrupting maize plant defenses and by excluding other fungi from necrotic tissues and crop residues. This is the first report of natural products from C. graminicola, as well as the production of monorden and monocillins by a pathogen of cereals.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28434294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Identification of heavy metal regulated genes from the root associated ascomycete Cadophora finlandica using a genomic microarray 用基因组芯片鉴定根相关子囊菌芬兰卡多夫重金属调控基因

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.09.005

Markus Gorfer, Helene Persak, Harald Berger , Sabine Brynda , Dragana Bandian, Joseph Strauss

{"title":"Identification of heavy metal regulated genes from the root associated ascomycete Cadophora finlandica using a genomic microarray","authors":"Markus Gorfer, Helene Persak, Harald Berger , Sabine Brynda , Dragana Bandian, Joseph Strauss","doi":"10.1016/j.mycres.2009.09.005","DOIUrl":"10.1016/j.mycres.2009.09.005","url":null,"abstract":"<div>The ascomycete Cadophora finlandica, which can form mycorrhizas with ectomycorrhizal and ericoid hosts, is commonly found in heavy metal polluted soils. To understand the selective advantage of this organism at contaminated sites heavy metal regulated genes from C. finlandica were investigated. For gene identification a strategy based on a genomic microarray was chosen, which allows a rapid, genome-wide screening in genetically poorly characterized organisms. In a preliminary screen eleven plasmids covering eight distinct genomic regions and encoding a total of ten Cd-regulated genes were identified. Northern analyses with RNA from C. finlandica grown in the presence of either Cd, Pb or Zn revealed different transcription patterns in response to the heavy metals present in the growth medium. The Cd-regulated genes are predicted to encode several extracellular proteins with unknown functions, transporters, a centaurin-type regulator of intracellular membrane trafficking, a GNAT-family acetyltransferase and a B-type cyclin.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.09.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28036571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Mycological Research News 真菌学研究新闻

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.11.001

引用次数: 0

Anthracnose disease of switchgrass caused by the novel fungal species Colletotrichum navitas 柳枝稷炭疽病的研究

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.09.010

Jo Anne Crouch, Lisa A. Beirn, Laura M. Cortese, Stacy A. Bonos, Bruce B. Clarke

{"title":"Anthracnose disease of switchgrass caused by the novel fungal species Colletotrichum navitas","authors":"Jo Anne Crouch, Lisa A. Beirn, Laura M. Cortese, Stacy A. Bonos, Bruce B. Clarke","doi":"10.1016/j.mycres.2009.09.010","DOIUrl":"10.1016/j.mycres.2009.09.010","url":null,"abstract":"<div>In recent years perennial grasses such as the native tallgrass prairie plant Panicum virgatum (switchgrass) have taken on a new role in the North American landscape as a plant-based source of renewable energy. Because switchgrass is a native plant, it has been suggested that disease problems will be minimal, but little research in this area has been conducted. Recently, outbreaks of switchgrass anthracnose disease have been reported from the northeastern United States. Incidences of switchgrass anthracnose are known in North America since 1886 through herbarium specimens and disease reports, but the causal agent of this disease has never been experimentally determined or taxonomically evaluated. In the present work, we evaluate the causal agent of switchgrass anthracnose, a new species we describe as Colletotrichum navitas (navitas  <!-->Latin for energy). Multilocus molecular phylogenetics and morphological characters show C. navitas is a novel species in the falcate-spored graminicolous group of the genus Colletotrichum; it is most closely related to the corn anthracnose pathogen Colletotrichum graminicola. We present a formal description and illustrations for C. navitas and provide experimental confirmation that this organism is responsible for switchgrass anthracnose disease.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.09.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40052222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 71

Two novel Peronospora species are associated with recent reports of downy mildew on sages 两个新的Peronospora种与最近报道的圣人霜霉病有关

Mycological research Pub Date : 2009-12-01 DOI: 10.1016/j.mycres.2009.08.010

Young-Joon Choi , Hyeon-Dong Shin , Marco Thines

{"title":"Two novel Peronospora species are associated with recent reports of downy mildew on sages","authors":"Young-Joon Choi , Hyeon-Dong Shin , Marco Thines","doi":"10.1016/j.mycres.2009.08.010","DOIUrl":"10.1016/j.mycres.2009.08.010","url":null,"abstract":"<div>Recently, downy mildew of Salvia species became economically relevant globally, but the taxonomy of the causal agent remains still obscure. The objective of this study was to characterize and distinguish the different Peronospora species associated with downy mildew on sages, based on morphological and molecular data. For this purpose we compared Peronospora specimens on Salvia officinalis and Salvia plebeia with Peronospora swinglei, including the type specimen on Salvia reflexa. We observed that three Peronospora species are associated with downy mildew on sages, and the recent outbreak of the disease on S. officinalis and S. plebeia is associated with two undescribed species, contrasting the view that either P. swinglei or Peronospora lamii is the causal agent of the downy mildew disease as claimed previously by several studies. In the study presented here, we provide the formal descriptions and illustrations for the two newly introduced taxa, Peronospora salviae-plebeiae and Peronospora salviae-officinalis. The three species of Peronospora investigated seem to be restricted to specific species of Salvia: P. swinglei to S. reflexa, P. salviae-plebeiae to S. plebeia, and P. salviae-officinalis to S. officinalis. Speciation may be allopatric and closely linked to the geographic distributions of their host plants.</div>","PeriodicalId":19045,"journal":{"name":"Mycological research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mycres.2009.08.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28431814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50