Molecules最新文献_第9页

Current Advances in the Use of Mushrooms as Therapeutics for Lung Cancer: A Review.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061322

Edward Thato Khunoana, Sanah Malomile Nkadimeng

{"title":"Current Advances in the Use of Mushrooms as Therapeutics for Lung Cancer: A Review.","authors":"Edward Thato Khunoana, Sanah Malomile Nkadimeng","doi":"10.3390/molecules30061322","DOIUrl":"10.3390/molecules30061322","url":null,"abstract":"Medicinal mushrooms have become increasingly important in the pharmaceutical industry because they contain a wealth of bioactive compounds and offer various nutritional benefits. These qualities also contribute to their widespread use in cooking. Global mycologists have suggested that a deeper understanding of mushrooms can aid in treating a variety of cancers at different stages. The excellent anticancer potential of fungi has inevitably attracted the attention of researchers, given the ever-increasing number of cases of lung cancer. Thus, the purpose of this review was to compile and synthesize the existing scientific literature about the potential of mushroom extracts particularly towards lung cancer prevention. A comprehensive literature search was conducted in electronic databases to identify relevant studies for the review. We found that 26 distinct kinds of mushrooms, prepared in over 10 different solvents, were used to make extracts that decreased the viability of different types of lung cancer cells (A549, NCI-H460, 3LL, H1264 cells etc.). We also examined a range of experimental models, including cell cultures (in vitro), mouse models (in vivo), as well as case studies and randomized controlled trials. The investigated mushrooms' effective mechanisms included: a reduction in the growth of cancer cells; an imbalanced percentage of cells in different phases of the cell cycle; an increase in autophagy and phagocytosis; an enhanced immune response; and the induction of cell apoptosis through the upregulation of pro-apoptotic factors and the downregulation of anti-apoptotic genes. Considering that mushrooms are consumed as a daily supplement, their potent pro-apoptotic properties and high antiproliferative efficacy are advantageous and could provide a model for further studies in this field as well as novel drug therapies and cancer treatments.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design, Synthesis, and Biological Evaluation of 5,8-Dimethyl Shikonin Oximes as SARS-CoV-2 M^pro Inhibitors.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061321

Jiahua Cui, Shouyan Xiang, Qijing Zhang, Shangqing Xiao, Gaoyang Yuan, Chenwu Liu, Shaoshun Li

{"title":"Design, Synthesis, and Biological Evaluation of 5,8-Dimethyl Shikonin Oximes as SARS-CoV-2 Mpro Inhibitors.","authors":"Jiahua Cui, Shouyan Xiang, Qijing Zhang, Shangqing Xiao, Gaoyang Yuan, Chenwu Liu, Shaoshun Li","doi":"10.3390/molecules30061321","DOIUrl":"10.3390/molecules30061321","url":null,"abstract":"We have designed, synthesized, and characterized a small library of shikonin derivatives and demonstrated their inhibitory activity against the main protease, Mpro, of SARS-CoV-2. One analog, 5,8-dimethyl shikonin oxime (15), exhibited the highest activity against SARS-CoV-2 Mpro with an IC50 value of 12.53 ± 3.59 μM. It exhibited much less toxicity as compared with the parent compound, shikonin, in both in vitro and in vivo models. Structure-activity relationship analysis indicated that the oxime moieties on the naphthalene ring and the functional groups attached to the oxygen atom on the side chain play a pivotal role in enzymatic inhibitory activity. Molecular docking results implied that the inhibitor 15 is perfectly settled in the core of the substrate-binding pocket of Mpro by possibly interacting with three catalytic residues, His41, Cys145, and Met165. Overall, the shikonin oxime derivative 15 deserves further investigation as an antiviral agent against SARS-CoV-2.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic Insights into Glycerol Oxidation to High-Value Chemicals via Metal-Based Catalysts.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061310

Junqing Li, Ying Tu, Kelin He, Chao Chen, Lixing Liang, Chongze Ruan, Qitao Zhang

{"title":"Mechanistic Insights into Glycerol Oxidation to High-Value Chemicals via Metal-Based Catalysts.","authors":"Junqing Li, Ying Tu, Kelin He, Chao Chen, Lixing Liang, Chongze Ruan, Qitao Zhang","doi":"10.3390/molecules30061310","DOIUrl":"10.3390/molecules30061310","url":null,"abstract":"The oxidation of glycerol offers a valuable route for producing high-value chemicals. This review provides an in-depth analysis of the current advancements and mechanistic insights into novel metal-based catalysts for glycerol oxidation. We discuss the catalytic roles of both precious metals (e.g., Pt, Pd, Au), noted for their high efficiency and selectivity, and cost-effective alternatives, such as Ni, Cu, and Fe. Bimetallic and metal oxide catalysts are highlighted, emphasizing synergistic effects that enhance catalytic performance. This review elucidates the key mechanism involving selective adsorption and oxidation, providing detailed insights from advanced spectroscopic and computational studies into the activation of glycerol and stabilization of key intermediates, including glyceraldehyde and dihydroxyacetone. Additionally, selective carbon-carbon bond cleavage to yield smaller, valuable molecules is addressed. Finally, we outline future research directions, emphasizing the development of innovative catalysts, deeper mechanistic understanding, and sustainable process scale-up, ultimately advancing efficient, selective, and environmentally friendly catalytic systems for glycerol valorization.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Adsorption of Ru-Based Dyes on the TiO₂ Surface to Enhance the Photovoltaic Efficiency of Dye-Sensitized Solar Cell Devices.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061312

Malgorzata Makowska-Janusik, Katarzyna Filipecka-Szymczyk, Daniel Pelczarski, Waldemar Stampor, Maciej Zalas

{"title":"The Adsorption of Ru-Based Dyes on the TiO2 Surface to Enhance the Photovoltaic Efficiency of Dye-Sensitized Solar Cell Devices.","authors":"Malgorzata Makowska-Janusik, Katarzyna Filipecka-Szymczyk, Daniel Pelczarski, Waldemar Stampor, Maciej Zalas","doi":"10.3390/molecules30061312","DOIUrl":"10.3390/molecules30061312","url":null,"abstract":"Adsorption of mononuclear tris(bipyridine) ruthenium(II) complexes and binuclear tris(bipyridine) ruthenium(II) complexes equipped with carboxyl groups (-COOH) on the (111) surface of TiO2 crystal in anatase form was modeled using Monte Carlo simulations, applying the Universal force field. It was shown that the adsorption efficiency of the ruthenium-based dyes on the TiO2 surface depends on the position of the anchoring -COOH group in the molecular structure. The increase in the number of possible anchor groups in the dyes increases their ability to deposit on the surface of semiconductors. The chemisorbed molecules, such as mononuclear tris(bipyridine) ruthenium(II) complexes with the -COOH group in para position (RuLp) and binuclear tris(bipyridine) ruthenium(II) complexes called B3 with two anchoring -COOH groups and phenyl in the spacer, interact with the adsorber and other neighboring dyes, changing their electron and optical properties. The obtained computational results help to explain the behavior of the dyes on the TiO2 surface, giving impact on their DSSC applications.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Measurements of Nanotrace Amounts of Lead and Cadmium Using an Environmentally Friendly Sensor (An Activated Glassy Carbon Electrode Modified with a Bismuth Film).

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061308

Katarzyna Tyszczuk-Rotko, Aleksy Keller

{"title":"Simultaneous Measurements of Nanotrace Amounts of Lead and Cadmium Using an Environmentally Friendly Sensor (An Activated Glassy Carbon Electrode Modified with a Bismuth Film).","authors":"Katarzyna Tyszczuk-Rotko, Aleksy Keller","doi":"10.3390/molecules30061308","DOIUrl":"10.3390/molecules30061308","url":null,"abstract":"This paper shows the fabrication of a new environmentally friendly sensor, an activated glassy carbon electrode with an in situ deposited bismuth film (aGCE/BiF), to determine Cd(II) and Pb(II) at the nanotrace level. The electrochemical activation of the GCE surface was achieved in a solution of 0.1 M phosphate-buffered saline (PBS) of pH = 7 by performing five cyclic voltammetric scans in the range of -1.5-2.5 V at ν of 100 mV/s. The newly developed electrode provides several advantages, such as an increased electron active surface (compared to the glassy carbon electrode) and improved electron transfer kinetics. As a result, the new voltammetric procedure (square-wave anodic stripping voltammetry, SWASV) was established and optimized. With the SWASV method, the following calibration curves and low detection limits (LODs) were obtained for Cd(II) and Pb(II), respectively: 5-100 nM, 0.62 nM, 2-200 nM, and 0.18 nM. The newly prepared method was used to determine the amounts of Pb(II) and Cd(II) in the certified reference material, and the results agreed with the certified values. Moreover, the procedure was successfully applied to determine the Cd(II) and Pb(II) in river samples. The official and standard addition methods validated the measurement results.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Potential Toxicity of Hyaluronic Acid-Coated Magnetic Nanoparticles on Maize (Zea mays) at Early Development Stages.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061316

Mihaela Răcuciu, Cristina-Nicoleta Precup, Maria Denisa Cocîrlea, Simona Oancea

{"title":"Assessment of Potential Toxicity of Hyaluronic Acid-Coated Magnetic Nanoparticles on Maize (Zea mays) at Early Development Stages.","authors":"Mihaela Răcuciu, Cristina-Nicoleta Precup, Maria Denisa Cocîrlea, Simona Oancea","doi":"10.3390/molecules30061316","DOIUrl":"10.3390/molecules30061316","url":null,"abstract":"The effectiveness of iron oxide nanoparticles in enhancing crop plant development depends on their stabilization. In this study, the effect of hyaluronic acid (HA), used both as a stabilizer for iron oxide nanoparticles (HA-MNP) and independently, was evaluated in maize seedlings. Different concentrations of HA-MNP (0.625-7.5 mg/L) were tested alongside a 0.01% HA solution. Growth parameters, antioxidant enzyme activities (peroxidase and polyphenol oxidase), photosynthetic pigments (chlorophyll and carotenoids), phenolic content, and genotoxicity were analyzed. While HA alone led to slight decreases in seedling length, pigment content, and polyphenol levels compared to the control, it increased peroxidase activity and mitotic index. Lower concentrations of HA-MNP (below 2.5 mg/L) enhanced seedling growth, likely due to improved iron uptake, whereas higher concentrations reduced pigment and phenolic content. All HA-MNP concentrations induced genotoxic effects, which was proven by an increased mitotic index and chromosomal aberrations, indicating both positive and defensive plant responses to oxidative stress. These findings suggest a complex interaction between HA, HA-MNP, and maize seedlings, where HA concentrations play a significant role in modulating growth and stress response, while higher concentrations may induce toxicity.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond Spin Crossover: Optical and Electronic Horizons of 2,6-Bis(pyrazol-1-yl)pyridine Ligands and Complexes.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061314

Yuliia Oleksii, Abdelkrim El-Ghayoury

{"title":"Beyond Spin Crossover: Optical and Electronic Horizons of 2,6-Bis(pyrazol-1-yl)pyridine Ligands and Complexes.","authors":"Yuliia Oleksii, Abdelkrim El-Ghayoury","doi":"10.3390/molecules30061314","DOIUrl":"10.3390/molecules30061314","url":null,"abstract":"The 2,6-bis(pyrazol-1-yl)pyridine (bpp) ligand family is widely recognized for its versatile coordination abilities and broad functionalization potential. This review examines bpp and its modifications at the pyridine ring's 4-position, focusing on their influence on magnetic, optical, and electronic properties. Key applications discussed include spin crossover (SCO), single-ion and single-molecule magnetism (SIM and SMM), luminescence, redox flow batteries (RFBs), and photonic devices. We provide a comprehensive overview of ligand modifications involving carboxylates, extended aromatic systems, radicals, and redox-active units such as tetrathiafulvalene (TTF), alongside supramolecular architectures. The review highlights fundamental design principles, particularly the role of substituents in tuning the SCO behavior, photophysical properties, and self-assembly into functional nanostructures. Notable advancements include SCO-driven conductivity modulation, reversible luminescent switching, and amphiphilic bpp-based vesicles for multicolor emission. By analyzing the interplay between ligand structure and magnetic, optical, and electronic functions, we provide insights into the potential of bpp derivatives for advanced materials design. This review presents recent experimental and theoretical developments, offering a foundation for future exploration of bpp-based compounds in multifunctional devices.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BI 1015550 Improves Silica-Induced Silicosis and LPS-Induced Acute Lung Injury in Mice.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061311

Yuming Liu, Jing Zhang, Yayue Hu, Zhigang Liu, Zhongyi Yang, Ran Jiao, Xueze Liu, Xiaohe Li, Feng Sang

{"title":"BI 1015550 Improves Silica-Induced Silicosis and LPS-Induced Acute Lung Injury in Mice.","authors":"Yuming Liu, Jing Zhang, Yayue Hu, Zhigang Liu, Zhongyi Yang, Ran Jiao, Xueze Liu, Xiaohe Li, Feng Sang","doi":"10.3390/molecules30061311","DOIUrl":"10.3390/molecules30061311","url":null,"abstract":"Silicosis is an interstitial lung disease (ILD) caused by prolonged inhalation of silica particles. Acute lung injury (ALI) is a critical clinical syndrome involving bilateral lung infiltration and acute hypoxic respiratory failure. However, there is currently no effective treatment for these two diseases. Previous research has established that cyclic adenosine monophosphate (cAMP) is pivotal in the pathogenesis of silicosis and acute lung injury. Phosphodiesterase 4 (PDE4) is a hydrolase enzyme of cAMP, and BI 1015550, as an inhibitor of PDE4B, is expected to be a candidate drug for treating both. BI 1015550 has shown certain anti-inflammatory and anti-fibrotic properties in systemic sclerosis-associated interstitial lung disease (SSc-ILD) and idiopathic pulmonary fibrosis (IPF), but there is a lack of research on silicosis and acute lung injury. In this research, we successfully synthesized BI 1015550 autonomously and demonstrated that it could significantly improve lung fibrosis and inflammation in a silica-induced silicosis mouse model. Furthermore, we found that BI 1015550 could also alleviate lung inflammation in a Lipopolysaccharide (LPS)-induced acute lung injury mouse model. The mechanism of action may involve the regulation of cAMP-related signaling pathways.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946787/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenolic and Immunometric Profiling of Wheat Varieties: Impact of Organic and Conventional Farming on Allergenic and Bioactive Compounds.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061313

Adrian Bartos, Alicja Malik, Beata Feledyn-Szewczyk, Krzysztof Jończyk, Renata Kazimierczak, Ewelina Hallmann, Joanna Leszczyńska

{"title":"Polyphenolic and Immunometric Profiling of Wheat Varieties: Impact of Organic and Conventional Farming on Allergenic and Bioactive Compounds.","authors":"Adrian Bartos, Alicja Malik, Beata Feledyn-Szewczyk, Krzysztof Jończyk, Renata Kazimierczak, Ewelina Hallmann, Joanna Leszczyńska","doi":"10.3390/molecules30061313","DOIUrl":"10.3390/molecules30061313","url":null,"abstract":"This study investigates the impact of organic and conventional farming on the allergenic and bioactive properties of wheat. The primary aim was to assess the immunometric parameters and polyphenolic composition in four varieties of winter and four varieties of spring wheat cultivated under both farming systems. Immunometric assays focused on gluten content, the allergenic QQQPP peptide, and the panallergenic profilin Tri a 12. While gluten levels (15-20 g/kg) showed no clear dependence on farming type, organic wheat exhibited a mild yet significant increase in QQQPP-dependent immunoreactivity in five samples (>20 µg/g). However, all organic wheat samples demonstrated a notable reduction in profilin content (<0.6 µg/g), suggesting that the type of wheat cultivation could influence allergenic risk for individuals with wheat-related allergies. Polyphenolic profiling revealed that kaempferol, p-coumaric acid, and gallic acid were the predominant compounds, with organic wheat displaying slightly higher polyphenol levels on average. Despite these differences, the variations were insufficient to determine a superior cultivation method. These findings highlight the potential allergenic and nutritional implications of organic versus conventional wheat farming.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11944287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart Probes for Ultrasensitive and Highly Selective Sensing of Homocysteine over Cysteine Based on Multi-Cooperative Effects by Using Gold Nanoparticles.

IF 4.2 2区化学

Molecules Pub Date : 2025-03-14 DOI: 10.3390/molecules30061309

Manman Sun, Peihao Zhang, Zeze Xie, Pengcheng Zhang, Zhendong Li, Zhiguang Yang, Hongyu Chen

{"title":"Smart Probes for Ultrasensitive and Highly Selective Sensing of Homocysteine over Cysteine Based on Multi-Cooperative Effects by Using Gold Nanoparticles.","authors":"Manman Sun, Peihao Zhang, Zeze Xie, Pengcheng Zhang, Zhendong Li, Zhiguang Yang, Hongyu Chen","doi":"10.3390/molecules30061309","DOIUrl":"10.3390/molecules30061309","url":null,"abstract":"Homocysteine (Hcy) is a biothiol that plays a vital role in many physiological processes and is involved in a variety of diseases. However, it is significantly difficult to discriminate Hcy from cysteine (Cys) due to their similar chemical structures (only one methylene difference) and reactivity. In this study, a novel nanosensor was proposed to discriminate Hcy from Cys with multi-cooperative effects by using gold nanoparticles (AuNPs). The discrimination effect for Hcy originates from the interaction difference of the hydrogen bonding, steric hindrance, and carbon chain length in Hcy and Cys with AuNPs. Under the best conditions, this nanosensor has two unique advantages. Firstly, the sensor exhibits high sensitivity with detection limits of 0.1 μM through naked-eye determination and 0.008 μM through UV-vis spectroscopy analysis. Secondly, the sensor showed superior selectivity for Hcy over the other 16 natural amino acids (biothiol-containing Cys and glutathione (GSH)), and it is the first time to clearly distinguish Hcy from Cys (the Cys concentration is 40 times higher than Hcy). Furthermore, the system was further employed to detect Hcy in human serum, and the result was in agreement with that tested by clinicians via enzymatic assays, with acceptable recovery.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11946112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0