Molecules最新文献

{"title":"Extraction of Curcuminoids and Carvacrol with Biobased Ionic Liquids-Evaluation of Anti-Cancer Properties of Curcuminoid Extracts.","authors":"Chefikou Salami, Jean-Pierre Mbakidi, Sandra Audonnet, Sylvie Brassart-Pasco, Sandrine Bouquillon","doi":"10.3390/molecules30051180","DOIUrl":"10.3390/molecules30051180","url":null,"abstract":"<p><p>Six biobased ionic liquids were prepared from saturated fatty acids (octanoic, decanoic and dodecanoic acids) and choline with yields up to 90% following procedures respecting green chemistry principles. These ionic liquids were fully characterized (NMR, IR, elemental analysis, viscosimetry and TGA) and used as extraction solvents for bioactive compounds (curcuminoids and carvacrol) using classical conditions, and the ionic liquids were able to be recovered after five runs without loss of activity. The ionic liquid containing a C12 carbon chain was the best extracting solvent, extracting 95% of the total curcuminoids contained in turmeric and 69% of the total carvacrol contained in oregano, which are higher yields compared to the extraction procedures described in the literature. As C12 ionic liquids were more cytotoxic than C8 ones, the biological activity of the curcuminoids extracted with C8 ionic liquids was evaluated on a MIAPaCa-2, a pancreatic adenocarcinoma cell line for which antitumor activity of curcuminoids had previously been reported. Compared to the cytotoxicity of the commercially available extract, the cytotoxic activity of the extracts was slightly weaker.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Characterization and Bioactivities of Sericin Extracted from Silkworm Cocoons from Two Regions of Portugal.","authors":"Sara Reis, Carina Spencer, Cristina M Soares, Soraia I Falcão, Sónia P Miguel, Maximiano P Ribeiro, Lillian Barros, Paula Coutinho, Josiana Vaz","doi":"10.3390/molecules30051179","DOIUrl":"10.3390/molecules30051179","url":null,"abstract":"<p><p>Sericin has been characterized as demonstrating a variety of bioactivities, establishing it as a valuable resource for biomedical and pharmaceutical applications. The diverse biological activities of sericin are likely linked to its unique biochemical composition and properties. This study aimed to assess the effect of origin, seasonality, and amino acid composition on the bioactivity of sericin samples from two Portuguese regions compared to commercial sericin. The amino acid profile was analyzed using HPLC-FLD. Moreover, several bioactivities were assessed through in vitro assays, including antiproliferative effects, cell migration, antimicrobial activity, anticoagulant properties, antioxidant capacity, and anti-inflammatory effects. The results obtained in this work revealed that the origin and season affect the sericin amino acid profile. In its pure state, sericin exhibited a low content of free amino acids, with tyrosine being the most abundant (53.42-84.99%). In contrast, hydrolyzed sericin displayed a higher amino acid content dominated by serine (54.05-59.48%). Regarding bioactivities, the sericin tested did not demonstrate antioxidant or anti-inflammatory potential in the conducted tests. Notwithstanding, it showed antiproliferative activity in contact with human tumor cell lines at a minimum concentration of 0.52 mg/mL. Regarding antimicrobial activity, sericin had the capacity to inhibit the growth of the bacteria and fungi tested at concentrations between 5 and 10 mg/mL. Additionally, sericin demonstrated its capacity to prolong the coagulation time in pooled human plasma, indicating a potential anticoagulant activity. In addition, the origin and season also revealed their impact on biological activities, and sericin collected in Bragança in 2021 (S3) and 2022 (S4) demonstrated higher antiproliferative, antibacterial, and anticoagulant potentials. Future studies should focus on optimizing sericin's bioactivities and elucidating its molecular mechanisms for clinical and therapeutic applications.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Insights and Therapeutic Potential of <i>Chamaenerion angustifolium</i> and <i>Chamaenerion latifolium</i>.","authors":"Akmaral Kozhantayeva, Zhanar Iskakova, Manshuk Ibrayeva, Ardak Sapiyeva, Moldir Arkharbekova, Yerbolat Tashenov","doi":"10.3390/molecules30051186","DOIUrl":"10.3390/molecules30051186","url":null,"abstract":"<p><p>The <i>Chamaenerion</i> genus, particularly <i>Chamaenerion angustifolium</i> and <i>Chamaenerion latifolium</i>, is recognized for its rich phytochemical composition and extensive medicinal properties. These species are abundant in polyphenols, flavonoids, and tannins, which contribute to their potent antioxidant, antimicrobial, and anticancer activities. This review provides a comprehensive analysis of their phytochemical constituents, with an emphasis on how processing methods, including fermentation, influence bioactivity. Notably, fermentation enhances the levels of key bioactive compounds, such as oenothein B, gallic acid, and ellagic acid, thereby increasing their pharmacological potential. Additionally, this review evaluates the biological activities of <i>Chamaenerion</i> species in relation to their chemical composition, while also considering the limitations of current studies, such as the lack of in vivo or clinical trials. The literature for this review was sourced from scientific databases, including PubMed, Scopus, and ScienceDirect, covering research from 2010 to 2024. Future studies should focus on optimizing extraction methods, elucidating synergistic bioactivities, and conducting in-depth clinical trials to validate their efficacy and safety.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synbiotic Microencapsulation of <i>Lactobacillus</i> Strains from Mexican Fermented Beverages for Enhanced Probiotic Functionality.","authors":"Morayma Ramírez-Damián, Cynthia Garfias-Noguez, Luis G Bermúdez-Humarán, María Elena Sánchez-Pardo","doi":"10.3390/molecules30051185","DOIUrl":"10.3390/molecules30051185","url":null,"abstract":"<p><p>Synbiotics, which combine probiotics and prebiotics, represent an innovative approach to developing functional foods with enhanced health benefits compared to their individual components. This study focuses on the production of synbiotics through the microencapsulation of <i>Lactobacillus</i> strains isolated from traditional Mexican fermented beverages, contributing to the advancement of technologies for functional food development. Three <i>Lactobacillus</i> strains (<i>Lacticaseibacillus rhamnosus</i> LM07, <i>Lactiplantibacillus plantarum</i> LM19, and <i>Levilactobacillus brevis</i> LBH1070) were microencapsulated by spray-drying using a mixture of maltodextrin and gum arabic as wall materials and inulin as a prebiotic. The microencapsulation process achieved high survival rates (>90%), low moisture content (~5%), and low water activity (~0.3), ensuring long-term stability. Notably, the microencapsulated strains demonstrated improved tolerance to gastrointestinal conditions, enhanced adhesion properties, and increased antioxidant activity compared to non-microencapsulated strains. These results highlight the potential of microencapsulation as an innovative technology not only to preserve but also to enhance probiotic properties, facilitating the development of functional foods with improved health-promoting properties, extended shelf life, and stability at room temperature.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11902131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction and Analysis of Phenolic Compounds from Rocket: Development of a Green and Innovative DES-Based Extraction Method.","authors":"Vittoria Terrigno, Susanna Della Posta, Giorgia Pietrangeli, Teodora Chiara Tonto, Vittoria Locato, Laura De Gara, Chiara Fanali","doi":"10.3390/molecules30051177","DOIUrl":"10.3390/molecules30051177","url":null,"abstract":"<p><p><i>Eruca sativa</i> Mill. is an annual plant belonging to the Cruciferous family that is characterized by the presence of antioxidant bioactive molecules such as phenolic compounds. Their extraction is usually performed through solid-liquid extraction based on the use of organic solvent. Deep eutectic solvents (DESs) are new green solvents capable of increasing bioactive molecules yield if replaced with organic solvents. The aim of this work was to develop a green analytical method based on the use of DESs for the determination of phenolic compounds in rocket plants. The extraction optimization involved the selection of the best extraction solvent among different selected DESs and the study of the parameters that mainly affect the extraction yield: the quantity of water to add to the selected DES to reduce its viscosity, the matrix-to-solvent ratio, and the time and temperature of the extraction. ChCl-glucose (1:2 molar ratio) DES was selected as the extraction solvent under the following optimized conditions: 1:50 (<i>w</i>/<i>v</i>) as the matrix-to-solvent ratio; 30% of water was added to the DES; extraction time of 30 min; and extraction temperature of 50 °C. The rocket phenolic compounds profile was determined through a high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) analysis. The innovative green method was applied to real plant samples to determine the growth conditions that favored the accumulation of bioactive molecules.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901794/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polysaccharides with Arabinose: Key Players in Reducing Chronic Inflammation and Enhancing Immune Health in Aging.","authors":"Patricia Pantoja Newman, Brenda Landvoigt Schmitt, Rafael Moura Maurmann, Brandt D Pence","doi":"10.3390/molecules30051178","DOIUrl":"10.3390/molecules30051178","url":null,"abstract":"<p><p>Aging is associated with a decline in physiological performance leading to increased inflammation and impaired immune function. Polysaccharides (PLs) found in plants, fruits, and fungi are emerging as potential targets for therapeutic intervention, but little is known about their effects on chronic inflammation and aging. This review aims to highlight the current advances related to the use of PLs, with the presence of arabinose, to attenuate oxidative stress and chronic and acute inflammation, and their immunomodulatory effects associated with antioxidant status in monocytes, macrophages, and neutrophil infiltration, and leukocyte rolling adhesion in neutrophils. In addition, recent studies have shown the importance of investigating the 'major' monosaccharide, such as arabinose, present in several of these polysaccharides, and with described effects on gut microbiome, glucose, inflammation, allergy, cancer cell proliferation, neuromodulation, and metabolic stress. Perspectives and opportunities for further investigation are provided. By promoting a balanced immune response and reducing inflammation, PLs with arabinose or even arabinose per se may alleviate the immune dysregulation and inflammation seen in the elderly, therefore providing a promising strategy to mitigate a variety of diseases.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Li et al. Extraction of Polysaccharides from Root of <i>Pseudostellaria heterophylla</i> (Miq.) Pax. and the Effects of Ultrasound Treatment on Its Properties and Antioxidant and Immune Activities. <i>Molecules</i> 2024, <i>29</i>, 142.","authors":"Hangyu Li, Ziwei Liu, Qianqian Liu, Xinnan Zhang, Sheng Li, Feng Tang, Linzi Zhang, Qian Yang, Qiran Wang, Shuyao Yang, Ling Huang, Yuwei Ba, Xihui Du, Falong Yang, Haibo Feng","doi":"10.3390/molecules30051174","DOIUrl":"10.3390/molecules30051174","url":null,"abstract":"<p><p><b>Errors in Figure</b> [...].</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern Comprehensive Metabolomic Profiling of Pollen Using Various Analytical Techniques.","authors":"Petra Krejčí, Zbyněk Žingor, Jana Balarynová, Andrea Čevelová, Matěj Tesárek, Petr Smýkal, Petr Bednář","doi":"10.3390/molecules30051172","DOIUrl":"10.3390/molecules30051172","url":null,"abstract":"<p><p>Pollen is a cornerstone of life for plants. Its durability, adaptability, and complex design are the key factors to successful plant reproduction, genetic diversity, and the maintenance of ecosystems. A detailed study of its chemical composition is important to understand the mechanism of pollen-pollinator interactions, pollination processes, and allergic reactions. In this study, a multimodal approach involving Fourier transform infrared spectrometry (FTIR), direct mass spectrometry with an atmospheric solids analysis probe (ASAP), matrix-assisted laser desorption/ionization (MALDI) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) was applied for metabolite profiling. ATR-FTIR provided an initial overview of the present metabolite classes. Phenylpropanoid, lipidic, and carbohydrate structures were revealed. The hydrophobic outer layer of pollen was characterized in detail by ASAP-MS profiling, and esters, phytosterols, and terpenoids were observed. Diacyl- and triacylglycerols and carbohydrate structures were identified in MALDI-MS spectra. The MALDI-MS imaging of lipids proved to be helpful during the microscopic characterization of pollen species in their mixture. Polyphenol profiling and the quantification of important secondary metabolites were performed by UHPLC-MS in context with pollen coloration and their antioxidant and antimicrobial properties. The obtained results revealed significant chemical differences among Magnoliophyta and Pinophyta pollen. Additionally, some variations within Magnoliophyta species were observed. The obtained metabolomics data were utilized for pollen differentiation at the taxonomic scale and provided valuable information in relation to pollen interactions during reproduction and its related applications.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11902019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent Regulation in Layered Zn-MOFs for C₂H₂/CO₂ and CO₂/CH₄ Separation.","authors":"Xingyao Zhao, Xiaotong Chang, Caixian Qin, Xiaokang Wang, Mingming Xu, Weidong Fan, Qingguo Meng, Daofeng Sun","doi":"10.3390/molecules30051171","DOIUrl":"10.3390/molecules30051171","url":null,"abstract":"<p><p>The development of alternative adsorptive separation technologies is extremely significant for the separation of C₂H₂/CO₂ and CO₂/CH₄ in the chemical industry. Emerging metal-organic frameworks (MOFs) have shown great potential as adsorbents for gas adsorption and separation. Herein, we synthesized two layered Zn-MOFs, UPC-96 and UPC-97, with 1,2,4,5-tetrakis(4-carboxyphenyl)-3,6-dimethylbenzene (TCPB-Me) as a ligand via the solvent regulation of the pH values. UPC-96 with a completely deprotonated ligand was obtained without the addition of acid, exhibiting two different channels with cross-sectional sizes of 11.6 × 7.1 and 8.3 × 5.2 Ų. In contrast, the addition of acid led to the partial deprotonation of the ligand and afforded UPC-97 two types of channels with cross-sectional sizes of 11.5 × 5.7 and 7.4 × 3.9 Ų. Reversible N₂ adsorption isotherms at 77 K confirmed their permanent porosity, and the differentiated single-component C₂H₂, CO₂, and CH₄ adsorption isotherms indicated their potential in C₂H₂/CO₂ and CO₂/CH₄ separation.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrochar from Agricultural Waste as a Biobased Support Matrix Enhances the Bacterial Degradation of Diethyl Phthalate.","authors":"Emanuel Gheorghita Armanu, Simone Bertoldi, Matthias Schmidt, Hermann J Heipieper, Irina Volf, Christian Eberlein","doi":"10.3390/molecules30051167","DOIUrl":"10.3390/molecules30051167","url":null,"abstract":"<p><p>The hydrothermal carbonization (HTC) of biomass presents a sustainable approach for waste management and production of value-added materials such as hydrochar, which holds promise as an adsorbent and support matrix for bacterial immobilization applied, e.g., for bioremediation processes of sites contaminated with phthalate ester plasticizers such as diethyl phthalate (DEP). In the present study, hydrochar was synthesized from vine shoots (VSs) biomass employing the following parameters during the HTC process: 260 °C for 30 min with a 1:10 (<i>w</i>/<i>v</i>) biomass-to-water ratio. The resulting vine shoots hydrochar (VSs-HC) was characterized for porosity, elemental composition, and structural properties using Scanning Electron Microscopy (SEM), Energy-Dispersive X-ray Spectroscopy (EDX), and Raman spectroscopy. Elemental analysis confirmed the presence of key elements in the VSs structure, elements essential for char formation during the HTC process. The VSs-HC exhibited a macroporous structure (>0.5 μm), facilitating diethyl phthalate (DEP) adsorption, bacterial adhesion, and biofilm formation. Adsorption studies showed that the VSs-HC achieved a 90% removal rate for 4 mM DEP within the first hour of contact. Furthermore, VS-HC was tested as a support matrix for a bacterial consortium (<i>Pseudomonas</i> spp. and <i>Microbacterium</i> sp.) known to degrade DEP. The immobilized bacterial consortium on VSs-HC demonstrated enhanced tolerance to DEP toxicity, degrading 76% of 8 mM DEP within 24 h, compared with 14% by planktonic cultures. This study highlights VSs-HC's potential as a sustainable and cost-effective material for environmental bioremediation, offering enhanced bacterial cell viability, improved biofilm formation, and efficient plasticizer removal. These findings provide a pathway for mitigating environmental pollution through scalable and low-cost solutions.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 5","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901840/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}