Mycopathologia最新文献

{"title":"Molecular detection of medically relevant Sporothrix species in roadkilled wildlife in the Brazilian Atlantic forest.","authors":"Steffanie Skau Amadei, Julia Campos, Andressa Maria Rorato Nascimento de Matos, Keity Aparecida Speçato, Eloiza Teles Caldart, Ana Paula Frederico Rodrigues Loureiro Bracarense, Ferry Hagen, Zoilo Pires de Camargo, Anderson Messias Rodrigues","doi":"10.1007/s11046-026-01067-4","DOIUrl":"10.1007/s11046-026-01067-4","url":null,"abstract":"<p><p>The rapid expansion of zoonotic sporotrichosis in South America necessitates innovative surveillance strategies to identify natural ecological niches. Roadkill provides a unique, underutilized opportunity to monitor Sporothrix circulation within human-impacted landscapes. We conducted a molecular survey via a triplex probe quantitative real-time PCR (qPCR) assay targeting pathogenic Sporothrix species in 81 roadkilled vertebrates (mammals, birds, and reptiles) collected along highways BR-376 and PR-445 traversing the Atlantic Forest in Paraná, Brazil (2017-2023). Genomic DNA from visceral organs (heart, liver, lung, and spleen) was screened for fungal DNA. Sporothrix DNA was detected in 13.6% (11/81) of the samples. Sporothrix schenckii predominated, identified in wild mammals (Leopardus guttulus, Didelphis albiventris, and Lepus europaeus) and diverse birds (Colaptes melanochloros, Piaya cayana, and Selenidera maculirostris), indicating systemic exposure. Strikingly, S. brasiliensis was detected in avian hosts (Columbina picui, Crypturellus tataupa), challenging the thermal-exclusion hypothesis and implicating birds as potential aerial vectors. Furthermore, S. globosa was found in Dasyprocta spp. and was co-detected with S. brasiliensis in Columbina picui. Notably, a reptile (Oxyrhopus spp.) was positive for S. globosa and S. schenckii, expanding the known host spectrum, potentially via trophic transmission. Although distinguishing transient DNA carriage from active infection requires histopathological validation, these findings suggest that wildlife in fragmented corridors may function as reservoirs, biological amplifiers, or mechanical vectors rather than incidental hosts, reinforcing the blurring boundaries between sylvatic and synanthropic transmission cycles. In this context, integrating roadkill biosurveillance into a One Health framework is vital for tracking environmental pathogen loads and anticipating zoonotic spillover.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12979292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catheter-Associated Candiduria: Aggregates, Microscopy, and CFU Variability.","authors":"Stephan Steixner, Angelika Bauer, Rosa Bellmann-Weiler, Cornelia Lass-Flörl","doi":"10.1007/s11046-026-01060-x","DOIUrl":"10.1007/s11046-026-01060-x","url":null,"abstract":"<p><p>Diagnosis of catheter-associated candiduria is limited by absent colony-forming unit (CFU) thresholds and heterogeneous laboratory workflows, complicating patient management. Within a quality-improvement (QI) initiative we aimed to (1) implement and evaluate a standardised quantitative culture (QM) workflow for catheter urine, (2) compare QM with routine semi-quantitative culture (SQM) for CFU detection and reproducibility, and (3) assess whether visible urine aggregates predict Candida spp. presence. From February 2024 to February 2025, we analysed 222 yeast-positive urine samples from 74 catheterised patients. This pilot combined process standardisation, parallel SQM and QM testing, targeted microscopy of aggregate-containing samples, and species identification by CHROMID® Candida agar and MALDI-TOF. Agreement between methods, intra-patient CFU variability over three daily samples, and the predictive value of aggregates were assessed. Aggregates were present in 29/222 samples (13.1%), of which 13 (44.8%) contained Candida spp., mainly C. albicans. No reliable macroscopic features distinguished Candida-positive from -negative aggregates. QM and SQM showed poor agreement (Bowker p < 0.001, κ = 0.17). QM detected growth in samples reported as \"sterile\" by SQM and provided continuous CFU counts. Cohort-level median QM counts were stable, but 13 patients (17.6%) showed notable variability, with 5 (6.8%) fluctuating > 10³ CFU/mL. Process data suggested batching and variable pre-analytics as contributors to discordance. A standardised QM workflow revealed substantial discordance with SQM and highlighted pre-analytical variability. Visible aggregates are unreliable indicators for Candida spp. presence. Adoption of standardised quantitative culture, systematic microscopy, and structured lab-clinician communication may improve diagnostic consistency; prospective evaluation is warranted.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12975776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Vertical Flow Dot-Immunobinding Assay (Dot-Iba) for Rapid Detection of Neoscytalidium dimidiatum.","authors":"Akkarapong Plengpanich, Sumanas Bunyaratavej, Anchalee Tungtrongchitr, Nawannaporn Saelim, Thapani Srisai, Charussri Leeyaphan, Piriyaporn Chongtrakool, Pichet Ruenchit","doi":"10.1007/s11046-026-01066-5","DOIUrl":"10.1007/s11046-026-01066-5","url":null,"abstract":"<p><p>Neoscytalidium dimidiatum is a non-dermatophyte mold that commonly causes skin and nail infections in tropical regions and often resists conventional antifungal therapies. Because its clinical and laboratory features often resemble dermatophyte infections, diagnosis is frequently delayed and treatment is sometimes inappropriate. We therefore developed a dot-immunobinding assay (Dot-Iba) to detect N. dimidiatum antigens. We generated a highly specific monoclonal antibody, 3E6F7 (MAb 3E6F7), for antigen capture, and used goat anti-mouse Ig conjugated with alkaline phosphatase (AP) as the signal generator. The test pad comprised a test hole, a nitrocellulose membrane (NC), and water-absorbent pads in a vertical flow-through format to allow a rapid antigen-antibody reaction. The assembled system detected N. dimidiatum antigens in vitro with high specificity and yielded visible results within 2 h; its detection limit was 0.9 µg without cross-reactivity to dermatophyte or non-dermatophyte fungi. This rapid, specific, and easy-to-use assay shows strong potential as a diagnostic tool, particularly in settings with limited access to fungal culture or advanced molecular diagnostics, where early, accurate identification is crucial.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12975793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemolytic and Co-Hemolytic Activity of Trichophyton indotineae and Related Dermatophytes Isolated from Chronic and Recalcitrant Infections.","authors":"Oğuzhan Bingöl, Deniz Alkaya, Aylin Döğen, Murat Durdu, Macit Ilkit","doi":"10.1007/s11046-026-01062-9","DOIUrl":"10.1007/s11046-026-01062-9","url":null,"abstract":"<p><p>Dermatophyte infections have evolved in both clinical presentation and etiological profiles, with chronic, recurrent, and widespread forms becoming increasingly prevalent and therapeutically challenging over the past decade. These infections are predominantly caused by Trichophyton indotineae (ITS genotype VIII), a member of the Trichophyton mentagrophytes complex. The virulence mechanisms of T. indotineae remain poorly understood. To elucidate potential pathogenic factors, we investigated hemolytic and co-hemolytic (CAMP-like) activities in dermatophytes associated with recalcitrant dermatophytosis. A total of 127 isolates (117 T. indotineae, 5 T. rubrum, 3 T. tonsurans, and 2 Microsporum canis) were examined using Columbia agar supplemented with 5% ovine (COA) or equine erythrocytes (CEA). Hemolysis was more consistently observed on COA (119 isolates) than on CEA (44 isolates) following 7-day incubation at 27 °C. Additional incubation at 36 °C up to 7 days was required to enhance hemolysis detection on CEA. Overall, 93.7% of isolates exhibited hemolytic activity, whereas only 3.1% demonstrated co-hemolytic activity. Among T. indotineae isolates, 97.4% were hemolytic. These findings underscore the importance of characterizing virulence traits in dermatophytes. Additional studies are needed to clarify the role of hemolysis in chronic and recalcitrant infections, with the goal of identifying novel therapeutic targets.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12968095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive Fusariosis Among Patients with Acute Leukemia: A 12-Year Single-Center Experience in a Middle-Income Country.","authors":"Raquel Keiko de Luca Ito, Patricia Rodrigues Bonazzi Pontes, Adriana Satie Goncalves Kono Magri, Karim Yaqub Ibrahim, Odeli Nicole Encinas Sejas, Jessica Toshie Katayose Goto, Juliana Pereira, Marcia de Souza Carvalho Melhem, Marcello Mihailenko Chaves Magri, Edson Abdala","doi":"10.1007/s11046-026-01068-3","DOIUrl":"10.1007/s11046-026-01068-3","url":null,"abstract":"<p><strong>Introduction: </strong>Invasive fusariosis (IF) is one of the most aggressive mold infections in patients with acute leukemia, characterized by rapid dissemination, high rates of fungemia, and limited antifungal susceptibility. Its impact is particularly severe in middle-income countries, yet single-center data from these settings remain scarce.</p><p><strong>Materials and methods: </strong>We conducted a retrospective study of all patients with acute leukemia and proven IF, diagnosed according to EORTC/MSG criteria, from January 2011 to August 2023. Demographic, clinical, therapeutic, and outcome variables were analyzed.</p><p><strong>Results: </strong>Twenty-six patients were identified. Median age was 46.5 years, and 53.8% were female. Acute myeloid leukemia was the most frequent underlying condition. All patients presented with severe neutropenia at diagnosis. Disseminated disease occurred in 84.6% of cases; two patients had isolated fungemia and two had localized skin disease. Cutaneous lesions were present in 73% of patients, and 31% had pulmonary involvement. Antifungal prophylaxis, mainly fluconazole or anidulafungin, was used in 69.2% of cases. Treatment consisted of lipid formulations of amphotericin B, either alone or combined with voriconazole. Thirty-day mortality reached 38.5%. Mortality was significantly higher among patients undergoing re-induction therapy, whereas those who had received antifungal prophylaxis exhibited lower mortality.</p><p><strong>Discussion: </strong>IF in acute leukemia was associated with extensive dissemination and substantial early mortality. Mortality rates were higher in patients in re-induction therapy and lower in those who received antifungal prophylaxis. The role of combination antifungal therapy requires further investigation.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12967573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147373048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraspecific Variations and Characteristics of Clinical, Physiological, and Antifungal Susceptibility Profiles of Trichophyton rubrum in Southwest China.","authors":"Yanni Deng, Wanglan Luo, Hao Hu, Jing Zhou, Qian Zhang, Songgan Jia, Wei Lei, Zhengling Shang, Yinhui Jiang, Zhu Zeng, Yanping Jiang","doi":"10.1007/s11046-026-01064-7","DOIUrl":"10.1007/s11046-026-01064-7","url":null,"abstract":"<p><p>To date, research on the efficacy of intraspecific molecular markers for the clonal fungal species Trichophyton rubrum, particularly studies that validate these markers through phenotypic characterization, has remained extremely limited. This study re-evaluated and confirmed the population structure of T. rubrum in southwestern Guizhou, China, by employing both the terg_02941 gene-encoding a protein of unknown function-and the conventional molecular marker rpb2. Integrated analyses incorporating clinical, physiological, and antifungal susceptibility data were conducted to investigate potential associations between distinct genetic lineages and their corresponding phenotypes. Phylogenetic analysis based on terg_02941 classified 324 Guizhou isolates into two major genetic lineages: ST1 (166 isolates) and ST2 (158 isolates), with ST1 forming a distinct lineage supported by moderate bootstrap values. In the rpb2-based phylogeny, two Guizhou isolates harboring non-synonymous mutations clustered within the ST1 group as a separate sublineage, further supporting the genetic stability or adaptive advantage of the ST1 population. Clinically, the ST1 lineage exhibited reduced prevalence among individuals under 15 years and over 60 years of age and showed diminished colonization of the human body's midline anatomical sites (P < 0.01). Physiologically, the ST1 population demonstrated lower tolerance to low temperature and acidic conditions. Notably, colonies of the ST1 lineage lacked red pigment production and predominantly exhibited yellow pigmentation (P < 0.001). Although no significant differences were observed in the geometric mean MIC values or the proportion of non-wild-type strains between the two lineages, pigment formation was negatively correlated with the degree of antifungal resistance (DDR) (P < 0.001), with classical red-pigmented strains showing the highest frequency of susceptibility. Collectively, the two globally distributed genetic lineages of T. rubrum in Guizhou exhibit both shared polymorphic traits and lineage-specific phenotypic tendencies. The more stable ST1 lineage appears to have achieved persistent colonization of human skin through a process of phenotypic streamlining, whereas the ancestral ST2 lineage displays broader phenotypic diversity, offering potential evidence for an ecological transition from environmental reservoirs to human host adaptation.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Susceptibility and Multilocus Sequence Typing of Pichia kudriavzevii (Candida krusei) from a Tertiary Hospital in Shanghai.","authors":"Guo Chen, Li Li, Junhao Zhu, Yixin Huang, Jieyi Wan, Demin Han, Min Zhu","doi":"10.1007/s11046-026-01061-w","DOIUrl":"10.1007/s11046-026-01061-w","url":null,"abstract":"<p><p>The incidence of infections by non-albicans Candida species, including Pichia kudriavzevii (formerly Candida krusei) has been increasing. P. kudriavzevii exhibits intrinsic resistance to fluconazole (FLU) and rapidly develops acquired resistance to other antifungals. However, large-scale epidemiological, antifungal susceptibility, and genotyping data from mainland China remain limited. We analyzed the clinical and laboratory data of 203 P. kudriavzevii isolates spanning from 2017 to 2023. In vitro antifungal susceptibility testing was performed by the Sensititre YeastOne microdilution method. The genotyping of P. kudriavzevii isolates was analysed by multilocus sequence typing (MLST). The results showed that P. kudriavzevii were predominantly isolated from sputum (132, 65.02%) and urine (21, 10.34%). Patients with P. kudriavzevii isolation were mainly from the Infectious Diseases Department (50, 24.63%), Intensive Care Unit (lCU) (45, 22.17%) and General Surgery (32, 15.76%), and the pathogen was highly prevalent in individuals over 60 years old. All isolates exhibited intrinsic resistance to FLU. The resistance rate to voriconazole (VOR) was 9.4% (19/203), showing an increasing trend. 80 isolates (39.41%) were non-wild-type (NWT) to amphotericin B (AMB) and posaconazole (POS). Excluding FLU, 31 isolates (15.27%) exhibited a resistant or NWT phenotype to at least two distinct antifungal agent classes, and the MLST analysis of them revealed 16 distinct diploid sequence types (DST) profiles. Among these, ST195 (7, 22.6%) and ST268 (6, 19.4%) were the predominant genotypes associated with antifungal resistance, and 58.1% of the resistant sequence types exhibited resistance to VOR. Continuous monitoring of antifungal susceptibility and molecular typing is essential. This study provides valuable insights for managing P. kudriavzevii infections and guiding clinical treatment strategies.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Mycological Profiles of Chronic and Recalcitrant Dermatophytosis: Türkiye, 2022-2024.","authors":"Murat Durdu, Hazal Kandemir, Ayşe Sultan Karakoyun, Pavlína Lysková, Daniela Kolarczyková, Oğuzhan Bingöl, Yasemin Erdem, Funda Aktürk-Gözel, Özlem Su-Küçük, Melisa Özay, Aslan Yürekli, İlayda Muslu, İsa An, Sinan Özçelik, Tuğba Falay-Gür, Tuğba Tehçi, Abdullah Demirbaş, Gözde Ulutaş-Demirbaş, Tuğba Atak, Kaan Taşolar, Büşra Acar-Mantar, Mehmet Kamil Mülayim, Rebiay Kıran, Ayşe Tülin Mansur, İrem Yanatma, Sertaç Sever, Yeşim Yayla-Öztürk, Selami Aykut Temiz, Rabia Öztaş-Kara, Fatma Uzun, Ferit Kuşçu, Gonca Erköse-Genç, Zayre Erturan, Sybren de Hoog, Vit Hubka, Macit Ilkit","doi":"10.1007/s11046-026-01058-5","DOIUrl":"10.1007/s11046-026-01058-5","url":null,"abstract":"<p><p>This study investigated the treatment outcomes of 91 patients with chronic and recalcitrant dermatophytosis from 20 hospitals in Türkiye between June 2022 and March 2024, as well as the in vitro antifungal susceptibility and squalene epoxidase (SQLE) substitution profiles of the dermatophytes obtained from these patients. Molecular identification by sequencing 91 isolates, revealed 76 Trichophyton mentagrophytes ITS genotype VIII (TmVIII; also known as T. indotineae) isolates, 13 Trichophyton rubrum, one Trichophyton tonsurans, and one Microsporum canis. The Phe397Leu substitution predominated in the SQLE, and Tyr414His co-occurred in nine TmVIII isolates. Five novel substitutions were observed in TmVIII. Among 13 T. rubrum isolates, three showed different SQLE substitution patterns compared to the wild-type strain. EUCAST antifungal susceptibility testing revealed high minimum inhibitory concentrations (MICs) for terbinafine (TRB) (> 0.25 mg/L) and for fluconazole (FLZ) (≥ 16 mg/L), indicating resistance in most TmVIII isolates. Of the 91 patients, 83 responded to FLZ, itraconazole (ITZ), TRB, or voriconazole-based regimens. While adjunct therapy with FLZ and resveratrol (RES) led to improvement in 28/29 patients despite their high in vitro inhibition concentrations, ITZ and curcumin (CUR) therapy was successful in 3/3 patients. However, the in vitro efficiency of CUR could not be evaluated. These findings highlight the local occurrence of specific SQLE substitutions, underscore the importance of EUCAST MIC testing combined with SQLE sequencing for surveillance, and support further evaluation of RES and CUR as an adjunct in TRB-resistant TmVIII.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":"34"},"PeriodicalIF":4.1,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12916938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146220527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungaemia Caused by the Rare Yeast Starmerella sorbosivorans: A Case Report with Identification via Molecular Phylogenetic Analysis and MALDI-TOF MS.","authors":"Shunkichi Ikegaki, Yuka Nagatsuka, Sayaka Ban, Daiki Kano, Goh Ohji, Hiroshi Yamamoto, Kentaro Iwata, Yuhki Sato","doi":"10.1007/s11046-026-01057-6","DOIUrl":"10.1007/s11046-026-01057-6","url":null,"abstract":"<p><p>Invasive yeast infections are associated with high mortality rates, particularly in immunocompromised patients. While most cases of fungaemia are caused by Candida species, rare yeast infections occasionally occur. Here, we present the first documented case of a Starmerella sorbosivorans (= Candida sorbosivorans, homotypic synonym) bloodstream infection in a 39 year-old man with chronic intestinal pseudo-obstruction requiring parenteral nutrition via a tunnelled central venous catheter. The patient presented with fever and was diagnosed with catheter-related bloodstream infection. Initial biochemical identification suggested S. magnoliae, but MALDI-TOF mass spectrometry (MS) failed to provide a definitive identification. Molecular phylogenetic analysis using multiple loci (18S rDNA, internal transcribed spacer [ITS] region, 26S rDNA-D1/D2, TEF1α, and RPB2), combined with proteomic analysis using MALDI-TOF MS, confirmed the isolate as S. sorbosivorans, despite a low ITS sequence similarity (91.3%) to the type strain. The patient was successfully treated with sequential administration of micafungin and liposomal amphotericin B, along with removal of the catheter. Antifungal susceptibility testing revealed low minimum inhibitory concentrations (MICs) for micafungin, amphotericin B, and voriconazole, but high MICs for fluconazole. Our findings emphasise the importance of multilocus sequence analysis for accurate species identification, especially in rare yeast infections caused by uncommon species such as S. sorbosivorans, for which conventional methods may be insufficient. However, to improve the applicability of MALDI-TOF MS in clinical settings, the reference library should be expanded to include rare and emerging yeast species.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":"33"},"PeriodicalIF":4.1,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CARD9 Mutations in Patients with Fungal Infections: A Comprehensive Literature Review.","authors":"Luhuai Shi, Xiaohua Tao, Yangmin Gao","doi":"10.1007/s11046-026-01056-7","DOIUrl":"10.1007/s11046-026-01056-7","url":null,"abstract":"<p><p>CARD9 deficiency is characterized by predisposing to increased susceptibility to fungal infections, particularly invasive fungi and dermatophytes. In recent years, the incidence of fungal infections in patients with CARD9 deficiency is on the rise, and the types of fungal pathogens have become increasingly diverse. In addition, most of CARD9 deficiency patients with fungal disease were prone to recurrence and resistant to antifungal agents, which pose significant challenges to treatment. To investigate the association between CARD9 mutations and fungal infections, we conducted a systematic analysis of published case reports involving 102 CARD9 deficient patients with fungal infections. In this study, the main fungal infections in these patients were candidiasis (34.3%), dermatophytosis (27.5%) and phaeohyphomycosis (23.5%). The main disease-causing fungi were Candida species (36.3%) and Trichophyton species (18.6%). 43 different genetic alterations in the CARD9 gene were identified and the three most frequent mutations were D274fsX60 (21.6%), Q289X (17.7%) and Q295X (12.8%). Our analysis revealed significant geographic variations in the frequency and distribution of CARD9 mutations. In addition, the Q289X and D274fsX60 were predisposed to dermatophytosis and phaeohyphomycosis, respectively. These findings enhance our understanding of the molecular mechanism underlying infection susceptibility in patients with CARD9 deficiency.</p>","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"191 2","pages":"32"},"PeriodicalIF":4.1,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146195057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}