Mycopathologia最新文献

Emergent Aspergillus tamarii and Fusarium falciforme Keratitis. 突发性柽柳曲霉和镰状镰刀菌角膜炎。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-26 DOI: 10.1007/s11046-025-00952-8

Ayman K El Essawy, Abdul Rahman Al Amri

引用次数: 0

Cutaneous Cauliflower-Like Lesions in a Horse Caused by Scedosporium apiospermum. 马皮肤花菜样病变由尖种鳞状孢子虫引起。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-22 DOI: 10.1007/s11046-025-00951-9

Andrea Grassi, Maria Elena Turba, Lorella Maniscalco

引用次数: 0

Atypical Facial Sporotrichosis. 非典型面部孢子菌病。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-22 DOI: 10.1007/s11046-025-00953-7

Gaobo Ruan, Xiujiao Xia

引用次数: 0

Transcriptomics Uncovers Key Genes for Photodynamic Killing on Trichosporon asahii Biofilms. 转录组学揭示了朝日毛丝虫病生物膜光动力杀伤的关键基因。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-18 DOI: 10.1007/s11046-025-00949-3

Wanting Luo, Guoliang Wang, Hongyu Chang, Guiming Liu, He Zhu, Haitao Li

{"title":"Transcriptomics Uncovers Key Genes for Photodynamic Killing on Trichosporon asahii Biofilms.","authors":"Wanting Luo, Guoliang Wang, Hongyu Chang, Guiming Liu, He Zhu, Haitao Li","doi":"10.1007/s11046-025-00949-3","DOIUrl":"10.1007/s11046-025-00949-3","url":null,"abstract":"Background: The escalating threat of antifungal resistance stemming from Trichosporon asahii (T. asahii) biofilms necessitates the pursuit of innovative therapeutic strategies. Among these approaches, 5-aminolevulinic acid (ALA) photodynamic therapy (PDT), an emerging therapeutic modality, has exhibited promising potential in eradicating T. asahii biofilms.Methods: The inhibitory activity was evaluated by confocal laser scanning microscopy. To delve deeper into the efficacy of ALA-PDT in eliminating T. asahii biofilms, we conducted a comprehensive transcriptional analysis utilizing transcriptome sequencing.Results: ALA-PDT demonstrated a profound inhibitory effect on the viability of T. asahii biofilms. Our investigation unveiled 2720 differentially expressed genes following exposure to ALA-PDT. Subsequent meticulous scrutiny allowed for the annotation of genes with a ≥ twofold change in transcription, focusing on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. Particularly noteworthy were the upregulated genes associated with oxidation-reduction processes, oxidoreductase activity, and catalytic activity. Conversely, the downregulated genes were linked to ATP binding, protein phosphorylation, and protein kinase activity. Additionally, we observed a surge in the transcription of genes that may be involved in oxidative stress (e.g., A1Q1_05494) as well as genes that may be involved in morphogenesis and biofilm formation (e.g., A1Q1_04029, A1Q1_01345, A1Q1_08069, and A1Q1_01456) following ALA-PDT treatment.Conclusions: Our findings underscore the substantial impact of ALA-PDT on the transcriptional regulation of genes related to oxidative stress, morphogenesis, and biofilm formation, paving the way for novel therapeutic avenues in combating T. asahii biofilms.","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"190 3","pages":"42"},"PeriodicalIF":3.6,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12086123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Synergistic Antifungal Activity of Pentamidine and Auranofin Against Multidrug-Resistant Candida auris. 喷他脒与金嘌呤对多重耐药耳念珠菌的协同抗真菌活性研究。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-14 DOI: 10.1007/s11046-025-00948-4

Yasmim Isabel Retore, Fabíola Lucini, Simone Simionatto, Luana Rossato

{"title":"Synergistic Antifungal Activity of Pentamidine and Auranofin Against Multidrug-Resistant Candida auris.","authors":"Yasmim Isabel Retore, Fabíola Lucini, Simone Simionatto, Luana Rossato","doi":"10.1007/s11046-025-00948-4","DOIUrl":"https://doi.org/10.1007/s11046-025-00948-4","url":null,"abstract":"Background: Candida auris is a significant clinical concern due to its ability to cause outbreaks in healthcare settings and its common resistance to current treatments. This highlights the need for alternative therapies. Drug repurposing offers a promising approach, and the combination of pentamidine (antiprotozoal) and auranofin (anti-rheumatic) has shown potential antifungal activity against Candida species, including C. auris. This study aimed to evaluate the antifungal activity of pentamidine and auranofin, both individually and in combination, against C. auris.Methods: Minimum Inhibitory Concentrations (MICs) were determined following CLSI guidelines, and drug interactions were assessed using the checkerboard microdilution method. Additional evaluations included growth inhibition, antibiofilm activity, cell damage, sorbitol protection, and efflux pump inhibition. Nucleotide leakage and cell membrane permeability were analyzed using biochemical assays. In vivo efficacy was tested using a Tenebrio molitor larvae model infected with C. auris.Results: The MICs of pentamidine against C. auris ranged from 16 to 128 μg/mL, showing fungicidal activity. The combination with auranofin had a synergistic effect (FICI: 0.37) and exhibited a fungistatic effect in growth inhibition assays. Auranofin was most effective at inhibiting biofilm formation. Pentamidine impaired mitochondrial function, leading to cellular respiration issues and membrane damage. Efflux pump assays indicated activation by both drugs, potentially influencing resistance. In vivo tests showed both drugs significantly improved survival rates in infected larvae compared to fluconazole.Conclusion: In conclusion, pentamidine and auranofin, either individually or in combination, are promising treatments for C. auris and warrant further research into optimal dosing and combination strategies.","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"190 3","pages":"41"},"PeriodicalIF":3.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rohino-Orbital-Cerebral Mycosis (ROCM) Attributable to Candida krusei Infection After COVID-19. 新型冠状病毒感染后由克鲁氏念珠菌感染引起的rohino - orbito - cerebral Mycosis （ROCM）。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-10 DOI: 10.1007/s11046-025-00946-6

Yulian Dou, Aihua Yu, Ming Yan

引用次数: 0

Superficial Mycoses: A Mapping Through Bibliometric Research. 浅表真菌病：通过文献计量学研究绘制地图。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-05-05 DOI: 10.1007/s11046-025-00947-5

Jintong Wu, Zijie Tang, Su Wang, Yuxin Qiu, Xinyu Nie, Chengxin Li, Rui Wang

{"title":"Superficial Mycoses: A Mapping Through Bibliometric Research.","authors":"Jintong Wu, Zijie Tang, Su Wang, Yuxin Qiu, Xinyu Nie, Chengxin Li, Rui Wang","doi":"10.1007/s11046-025-00947-5","DOIUrl":"https://doi.org/10.1007/s11046-025-00947-5","url":null,"abstract":"Superficial mycosis is a common and recurrent infectious skin disease. It poses significant challenges, with high recurrence rates and drug resistance, which notably diminishes the quality of life for patients and presents substantial public health issues. Numerous publications on superficial mycosis have posed significant challenges for researchers to manage the overwhelming amount of information effectively. This study aims to comprehensively explore the current state and latest advancements in global research through bibliometric techniques, providing a holistic appraisal of the field. Publications from the Web of Science Core Collection database were analyzed, including publications and citations, author groups and their countries and regions, journal categories, publishing institutions, and keywords using Excel 2019, VOSviewer, and CiteSpace. A total of 2206 papers were reviewed, showing a stable increase in research output from 2020 to 2022 and a predicted growth trend. The United States and India published the most significant number of research papers. Key research areas identified were \"Outbreak\", \"Desorption ionization time\", \"Formulations\", \"Impact\", \"Dermatophyte\", and \"Dermoscopy\". This bibliometric analysis provides a comprehensive visualized map to describe current and development trends. Advanced diagnostic technologies and innovative delivery systems are key current research priorities and will remain focal areas in this field.","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"190 3","pages":"39"},"PeriodicalIF":3.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering Fluconazole Resistance in Candida auris clade V: The Role of Efflux Pump Gene Expression and Ergosterol Pathway Mutations. 解读耳念珠菌分支V对氟康唑的耐药性：外排泵基因表达和麦角甾醇途径突变的作用。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-04-17 DOI: 10.1007/s11046-025-00945-7

Robab Ebrahimi Barough, Mahdi Abastabar, Maryam Moazeni, Javad Javidnia, Reza Valadan, Azadeh Bandegani, Mohsen Nosratabadi, Iman Haghani, Bram Spruijtenburg, Darius Armstrong-James, Hamid Badali

{"title":"Deciphering Fluconazole Resistance in Candida auris clade V: The Role of Efflux Pump Gene Expression and Ergosterol Pathway Mutations.","authors":"Robab Ebrahimi Barough, Mahdi Abastabar, Maryam Moazeni, Javad Javidnia, Reza Valadan, Azadeh Bandegani, Mohsen Nosratabadi, Iman Haghani, Bram Spruijtenburg, Darius Armstrong-James, Hamid Badali","doi":"10.1007/s11046-025-00945-7","DOIUrl":"https://doi.org/10.1007/s11046-025-00945-7","url":null,"abstract":"Candida auris is an emerging multidrug-resistant yeast pathogen that poses a serious global health threat. In particular, fluconazole resistance is common in C. auris, posing challenges for treating invasive infections. Understanding the genetic and molecular mechanisms underlying fluconazole resistance in C. auris is crucial for developing effective control strategies. The current study investigated the genetic and molecular basis of fluconazole resistance in C. auris clade V isolates. Furthermore, we examined mutations in ergosterol biosynthesis genes and expression of efflux pump genes in fluconazole-resistant versus susceptible in strains Clade V. Two C. auris isolates, one fluconazole-resistant, and one fluconazole-susceptible, were subjected to qPCR analysis of efflux pump gene (CDR1, CDR2, MDR1, MDR2) expression. Protein structure modeling was also performed to assess the impact of mutation in the ergosterol biosynthesis gene (ERG11) on antifungal drug accessibility. qPCR analysis revealed no significant difference in the expression levels of the efflux pump genes CDR1, CDR2, and MDR1 between the resistant and susceptible strains. Protein structure modeling indicated that the Y132F mutation in ERG11 likely altered fluconazole binding and accessibility. This study provides insights into the genetic and molecular mechanisms underpinning fluconazole resistance in C. auris Clade V. The findings highlight the critical roles of ERG11 mutation in mediating azole resistance in this emerging fungal pathogen.","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"190 3","pages":"38"},"PeriodicalIF":3.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Keep the Hospital Clean: Diagnostic Performance of Ten Different Molecular and Culture-Based Methods to Detect Candidozyma (Candida) auris. 保持医院清洁：十种不同分子和培养方法检测耳念珠菌的诊断性能。

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-04-15 DOI: 10.1007/s11046-025-00944-8

Koos Korsten, Bert Gerrits van den Ende, Rick D Pique, Ferry Hagen, Karin van Dijk

{"title":"Keep the Hospital Clean: Diagnostic Performance of Ten Different Molecular and Culture-Based Methods to Detect Candidozyma (Candida) auris.","authors":"Koos Korsten, Bert Gerrits van den Ende, Rick D Pique, Ferry Hagen, Karin van Dijk","doi":"10.1007/s11046-025-00944-8","DOIUrl":"https://doi.org/10.1007/s11046-025-00944-8","url":null,"abstract":"Rationale: Candidozyma auris (formerly Candida auris) is a globally emerging potentially multi-drug resistant human pathogenic yeast. To detect C. auris we aimed to compare different culture-, and molecular-based methods.Methods: Rectal swabs routinely collected in clinical care were spiked with different concentrations of C. auris. Co-infection/colonization was mimicked by spiking part of these samples with other pathogenic Candida species. Spiked materials were cultured at 37 or 42 °C using CHROMagar Candida and CHROMagar Candida Plus plates. In parallel, samples were incubated in a dulcitol salt enrichment broth. Additionally, we compared seven in-house and commercial molecular tests on the direct material and from the broth one day after inoculation.Results: Culture-based methods showed sensitivities up to 100% within 48 h of incubation, although sensitivity decreased as low as 44% at lower concentrations (≤ 50 CFU per inoculum), in the presence of an abundance of other species and at higher temperature (42 °C). Incubation at 42 °C made visual identification possible since other species with similar colony morphologies did not grow at this temperature. No added value of using the dulcitol salt enrichment broth was found. qPCR on direct materials was highly sensitive and specific (both up to 100%) but major differences between various molecular tests were observed.Conclusion: We showed that both culture-based and molecular methods are sensitive for diagnosing C. auris. The clinical setting (routine screening versus an outbreak), local prevalence and the load in those that carry or are infected by C. auris are important factors to consider when determining which diagnostic tests should be employed.","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"190 3","pages":"37"},"PeriodicalIF":3.6,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12000201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Features of Invasive Fungal Disease in China Tertiary Hospital: A Prospective, Multicenter Study. 中国三级医院侵袭性真菌病的临床特征：一项前瞻性、多中心研究

IF 3.6 3区生物学

Mycopathologia Pub Date : 2025-04-11 DOI: 10.1007/s11046-025-00940-y

Xingye Meng, Xiao Liu, Li Li, Dongyan Zheng, Lingbing Zeng, Yanling Liu, Ruoyu Li, Min Zhu, Cunwei Cao, Xianwei Cao, Yinggai Song, Jin Yu

{"title":"Clinical Features of Invasive Fungal Disease in China Tertiary Hospital: A Prospective, Multicenter Study.","authors":"Xingye Meng, Xiao Liu, Li Li, Dongyan Zheng, Lingbing Zeng, Yanling Liu, Ruoyu Li, Min Zhu, Cunwei Cao, Xianwei Cao, Yinggai Song, Jin Yu","doi":"10.1007/s11046-025-00940-y","DOIUrl":"https://doi.org/10.1007/s11046-025-00940-y","url":null,"abstract":"Invasive fungal disease (IFD) has high morbidity and mortality, the spectrum of pathogenic fungi and high-risk groups have also changed. Fewer literature focus on the overall incidence of IFD in various departments of general hospitals. Among the adult inpatients in four Chinese tertiary hospitals located in Beijing, Shanghai, Nanning and Nanchang, proven or probable cases of IFD were included prospectively in this study between May 1, 2021 and May 1, 2022. The clinical data were collected and analyzed. A total of 330 patients (342 episodes) with 278 of proven and 64 of probable IFDs were included, including invasive candidiasis (IC) (132, 40.0%), cryptococcosis (64, 19.4%), invasive aspergillosis (IA) (54, 16.4%), Talaromyces marneffei (TsM) infection (43, 13.0%), Pneumocystis pneumonia (PCP) (16, 4.8%), mixed fungal infection (10, 3.0%), other mold or yeast infection. 37.9% occurred in elderly patients (age ≥ 65 years). Nosocomial infection accounted for 44.5%, the proportion of nosocomial infection was highest in patients with IC (81.8%). Diabetes (19.7%) was the most common underlying disease. 83.9% of the 342 episodes of IFD had evidence of fungal culture, while the proportion of microscopic examination and histopathology as mycological evidence was 26.9% and 3.5%, respectively. The cumulative all-cause mortality at 180 days after diagnosis of IFD was 38.5%. Age ≥ 65 years old (HR = 1.670, P = 0.009), ICU (HR = 2.002, P = 0.001), nosocomial infection (HR = 1.630, P = 0.016) and diabetes (HR = 1.679, P = 0.013) were associated with increased death in IFD patients. The prognosis of IFD patients was poor. Doctors should pay attention to nosocomial fungal infection especially in old and diabetes.","PeriodicalId":19017,"journal":{"name":"Mycopathologia","volume":"190 3","pages":"36"},"PeriodicalIF":3.6,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0