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Effect of long-term enhanced external counterpulsation on promotion of angiogenesis in coronary occlusion dogs 长期强化体外反搏对冠脉闭塞犬血管生成的促进作用
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.004
谢强, 伍贵富, 杜志民, 胡承恒, 方典秋, 戴刚, 郑振声
{"title":"Effect of long-term enhanced external counterpulsation on promotion of angiogenesis in coronary occlusion dogs","authors":"谢强, 伍贵富, 杜志民, 胡承恒, 方典秋, 戴刚, 郑振声","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.004","url":null,"abstract":"目的 探索长期体外反搏对冠状动脉闭塞犬心肌微血管和血管新生作用的影响以及对循环和心肌局部血管内皮生长因子(VEGF)表达的影响.方法 雄性Beagle犬12只,随机分为对照组(n=6)和反搏组(n=6),均用心导管法建立定向冠状动脉闭塞模型3 d后,反搏组接受体外反搏处理,每日1 h,持续共6周(每只犬反搏总时间28～30 h).血管细胞成分免疫标记技术比较心肌微血管的差异和血管新生作用.双抗体夹心ELISA法测定犬血清VEGF水平动态变化.免疫组化SP法(链霉菌抗生物素蛋白-过氧化酶免疫组化染色超敏法)测定心肌组织局部VEGF的表达.RT-PCR检测心肌组织局部VEGF Mrna的表达.结果 (1)图像分析证实,反搏组犬缺血心肌组织内的微血管的数量显著高于对照组[α-actin:(11.8±5.3)支/HP比(3.4 ±1.2)支/HP,P＜0.05 ;FⅧ-r-Ag :(15.2±6.3)支/HP比(4.9±2.1)支/HP, P＜0.05].(2)冠状动脉急性闭塞后,两组犬的血清VEGF水平开始升高,在24 h均达到一峰值水平,随后VEGF水平下降,约在1周时降至最低.之后两组犬的血清VEGF水平有轻微增加,但组间变化差异无统计学意义.(3)反搏组犬缺血心肌组织的VEGF表达范围较广泛,但绝大部分仍分布于心肌细胞,少数分布于血管内皮细胞和成纤维细胞.相反,在对照组,心肌VEGF阳性表达的范围和强度均不及反搏组.(4)免疫组化图像分析发现,反搏组犬心肌VEGF表达的面积[(0.0353±0.0090)mm2比(0.0036±0.0008)mm2,P＜0.01]、吸光度指数[(3.0391±0.5121)比(0.3473±0.0840),P＜0.01)]均高于对照组;对两组心肌组织VEGF Mrna的表达进行半定量分析,结果反搏组犬心肌VEGF Mrna的表达显著高于对照组(P＜0.01),增加73.5%.结论 长期体外反搏治疗促进冠状动脉侧支循环和新生血管形成,促进缺血心肌组织局部VEGF蛋白和Mrna水平的表达可能是体外反搏的治疗机制之一。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133313185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CD30+间变大细胞性淋巴瘤研究进展 CD30+间变大细胞性淋巴瘤研究进展
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.018
王步飞, 黄慧强
{"title":"CD30+间变大细胞性淋巴瘤研究进展","authors":"王步飞, 黄慧强","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.018","url":null,"abstract":"1985年,Stein等首先描述了一种表达CD30(Ki-1)的,以多形性大细胞增殖为特征的淋巴瘤,并命名为间变大细胞淋巴瘤(anaplastic large cell lymphoma, ALCL).ALCL是一种异质性疾病,根据临床表现、组织形态、免疫表型及细胞遗传学特征,可分为多种不同的类型.临床上,ALCL可为原发性的,也可以继发于进展性淋巴细胞增殖性疾病.原发ALCL又可以进一步分为原发系统型ALCL和原发皮肤型ALCL.从免疫表型看,ALCL可以来源于T细胞,或为非T非B细胞表型.从细胞遗传学来看,部分ALCL有t(2:5)染色体异常并构成一个独特的类型.本文主要介绍近5年来对原发系统型ALCL的细胞免疫学、分子遗传学、临床病理分类、治疗策略和预后因素的研究进展。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134641549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of double-balloon enteroscopy to diagnose obscure gastrointestinal bleeding 双气囊小肠镜诊断消化道隐蔽性出血的应用
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.012
詹琪, 李瑜元, 聂玉强
{"title":"Application of double-balloon enteroscopy to diagnose obscure gastrointestinal bleeding","authors":"詹琪, 李瑜元, 聂玉强","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.012","url":null,"abstract":"目的 研究双气囊推进式小肠镜在不明原因消化道出血诊断中的价值.方法 对本院2003年7月至2005年11月不明原因消化道出血患者的双气囊推进式小肠镜(double-balloon enteroscopy,DBE)检查结果进行回顾分析,取同期非出血患者的DBE检查作为对照.对出血和非出血性小肠病灶的阳性诊断率进行统计.结果 出血组38例,经口入镜22例,经肛入镜6例,分别从两端入镜10例,发现出血灶35例(92.1%),其中小肠出血31例(81.6%).8例经手术切除并证实诊断,2例经镜下治疗后止血.对照组50例,病灶发现37例(74%),病灶诊出率低于出血组(P＜0.05) .结论 双气囊推进式小肠镜是一种安全、可靠、有效的检查手段,对不明原因消化道出血有较高的诊断价值。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"106 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123415763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Examination of daytime sleepiness in patients with obstructive sleep apnea syndrome and development of automatic monitoring system 阻塞性睡眠呼吸暂停综合征患者日间嗜睡的检测及自动监测系统的研制
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.013
朱海锋, 杨智, 罗远明
{"title":"Examination of daytime sleepiness in patients with obstructive sleep apnea syndrome and development of automatic monitoring system","authors":"朱海锋, 杨智, 罗远明","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.013","url":null,"abstract":"目的 建立一项简单客观地评价阻塞性睡眠呼吸暂停(obstructive sleep apnea,OSA)患者白天嗜睡的方法.方法 先制作一套判断睡眠的自动化睡眠监控系统并用于醒觉维持试验(MWT) . 10例OSA患者和5名正常人进行2次相隔约1 h的MWT.记录睡眠多导图包括脑电图、眼动图和下颌肌电图.结果 建立了一套判断睡眠的自动化睡眠监控系统,由自动化睡眠监控系统检测的醒觉维持时间与睡眠多导图所检测的一致.OSA患者的醒觉维持时间是(21±7) min明显小于正常人(38±2)min(P＜0.01).结论 在MWT试验时,自动化睡眠监控系统可代替复杂的睡眠多导图。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126775829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
内皮祖细胞的研究进展 内皮祖细胞的研究进展
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.019
王峰, 田晶, 谭孟群
{"title":"内皮祖细胞的研究进展","authors":"王峰, 田晶, 谭孟群","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.019","url":null,"abstract":"出生后骨髓中存在着一群祖细胞能够迁移到外周循环中,并且可以分化为成熟的内皮细胞,这一群细胞被定义为内皮祖细胞(endothelial progenitor cells,EPCs).在胚胎发育的过程中,EPCs参与了最初的血管形成(vasculogenesis)。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130406230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced external counterpulsation introducing to the world 向世界推出增强的外部反搏
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.001
郑振声
{"title":"Enhanced external counterpulsation introducing to the world","authors":"郑振声","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.001","url":null,"abstract":"","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131206345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction and identification of PML-RARα/pIRES and PML-RARα/pSecTag recombinant vectors PML-RARα/pIRES和PML-RARα/pSecTag重组载体的构建与鉴定
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.015
汤冀, 李扬秋, 周羽竝, 杨力建, 陈少华, 胡刚, 罗更新
{"title":"Construction and identification of PML-RARα/pIRES and PML-RARα/pSecTag recombinant vectors","authors":"汤冀, 李扬秋, 周羽竝, 杨力建, 陈少华, 胡刚, 罗更新","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.015","url":null,"abstract":"目的 构建PML-RARα基因重组表达质粒,为发展PML-RARα基因疫苗提供实验依据. 方法利用RT-PCR方法从急性早幼粒细胞白血病细胞株NB4细胞中扩增出465 bp 的位于PML-RARα融合基因的框内结构的片段,克隆至真核表达载体pIRES和分泌载体pSecTag后,经酶切和序列分析鉴定重组质粒的构建情况.结果 经酶切鉴定和序列测定表明,PML-RARα目的基因已正确插入真核表达载体中,构成了目的重组载体. 结论成功构建了PML-RARα/pIRES和PML-RARα/pSecTag重组真核表达质粒,可进一步用于PML-RARα基因疫苗的实验研究。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121438124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
化学发光免疫分析法检测黄体生成素和癌胚抗原的性能评价 化学发光免疫分析法检测黄体生成素和癌胚抗原的性能评价
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-04-30 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.02.040
周强, 邱峰, 陈富
{"title":"化学发光免疫分析法检测黄体生成素和癌胚抗原的性能评价","authors":"周强, 邱峰, 陈富","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.02.040","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.02.040","url":null,"abstract":"目的 评价化学发光免疫分析法检测黄体生成素和癌胚抗原.方法通过对不同浓度样品黄体生成素(LH)、癌胚抗原(CEA)含量的测定,进行精密度、灵敏度、干扰、回收试验.结果批内CV均小于5%,批间CV均小于6.5%;LH、CEA回收率分别为97.9%～102.2%、98.9%～102.4%,灵敏度分别达到2.4IU/L、2.6ng/ml;无吸样间的交叉污染.结论该方法各项指标结果准确,精密度好、灵敏度高、干扰小,具有广泛的应用前景。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115402557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
术中^125I放射性种子源胸内植入在非小细胞肺癌治疗中的应用 术中^125I放射性种子源胸内植入在非小细胞肺癌治疗中的应用
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-04-30 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.02.030
陈汉章, 杨德康, 何建行, 韦兵, 葛林虎, 殷伟强, 成向阳
{"title":"术中^125I放射性种子源胸内植入在非小细胞肺癌治疗中的应用","authors":"陈汉章, 杨德康, 何建行, 韦兵, 葛林虎, 殷伟强, 成向阳","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.02.030","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.02.030","url":null,"abstract":"目的 探讨术中放射性种子源胸内植入在非小细胞肺癌治疗中的应用方法及适应证。方法 对14例非小细胞肺癌病人采用术中平面植入^125I放射性种子源进行组织间近距放射治疗并总结。结果 14例患者均采用^125I放射性种子源术中．平面植入。术后恢复良好，无术中死亡，随访13～25个月，13例未见局部复发，局部控制率93．0％。未见明显相关并发症发生。结论 术中放射性种子源胸内植入治疗非小细胞肺癌是一安全有效的方法，可有效地改善肿瘤局部控制率，近期疗效满意。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130209065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
婴儿先天性会厌囊肿的围术期处理 婴儿先天性会厌囊肿的围术期处理
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-04-30 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.02.037
王洁, 刘大波, 罗仁忠, 黄振云, 周丽枫, 钟建文
{"title":"婴儿先天性会厌囊肿的围术期处理","authors":"王洁, 刘大波, 罗仁忠, 黄振云, 周丽枫, 钟建文","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.02.037","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.02.037","url":null,"abstract":"目的 探讨提高婴儿先天性会厌囊肿围手术期的安全性,降低止并发症的发生.方法分析了14例先天性会厌囊肿患儿疗法及围术期处理.结果全麻后支撑喉镜下囊肿切除术加微波烧灼术.术中术后并发症少,手术效果好,随访6个月～1年未见复发.结论本病起病急、症状重、合并症多、位置隐蔽,易误诊和漏诊,应注意完善术前准备,掌握好手术时机,谨慎选择手术方式。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"53 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131805751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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