PML-RARα/pIRES和PML-RARα/pSecTag重组载体的构建与鉴定

Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI:10.3760/CMA.J.ISSN.1008-634X.2006.03.015

汤冀, 李扬秋, 周羽竝, 杨力建, 陈少华, 胡刚, 罗更新

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引用次数: 0

摘要

目的构建PML-RARα基因重组表达质粒,为发展PML-RARα基因疫苗提供实验依据. 方法利用RT-PCR方法从急性早幼粒细胞白血病细胞株NB4细胞中扩增出465 bp 的位于PML-RARα融合基因的框内结构的片段,克隆至真核表达载体pIRES和分泌载体pSecTag后,经酶切和序列分析鉴定重组质粒的构建情况.结果经酶切鉴定和序列测定表明,PML-RARα目的基因已正确插入真核表达载体中,构成了目的重组载体. 结论成功构建了PML-RARα/pIRES和PML-RARα/pSecTag重组真核表达质粒,可进一步用于PML-RARα基因疫苗的实验研究。

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Construction and identification of PML-RARα/pIRES and PML-RARα/pSecTag recombinant vectors

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Journal of Modern Clinical Medical Bioengineering

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