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Evaluation of thymic recent output function in patients with ANLL by quantitative analysis of TRECs content 定量分析trec含量评价ANLL患者胸腺近期输出功能
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.011
杨力建, 李扬秋, 陈少华, 耿素霞, 吴秀丽, 张学利
{"title":"Evaluation of thymic recent output function in patients with ANLL by quantitative analysis of TRECs content","authors":"杨力建, 李扬秋, 陈少华, 耿素霞, 吴秀丽, 张学利","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.011","url":null,"abstract":"目的 检测急性非淋巴细胞白血病(ANLL)患者 T细胞受体重排删除DNA环(signal joint T-cell receptor excision DNA circles sjTRECs,TRECs)的含量,从而探讨患者的初始型naive T细胞水平和胸腺近期输出功能特点.方法利用实时定量PCR(TaqMan)方法,检测77例ANLL患者外周血单个核细胞(PBMC)TRECs的水平,并根据外周血中CD3阳性率计算CD3细胞中TRECs水平.14例ANLL缓解期患者和14例正常人外周血作为对照.结果 ANLL患者外周血中TRECs含量为(0.43±0.92)拷贝/1 000 PBMCs, (2.02±3.25)拷贝/1 000 CD3+细胞,明显低于正常人TRECs水平[(4.10±3.65)拷贝/1 000 PBMCs和(6.84±4.71)拷贝/1 000 CD3+细胞,P=0.0000和P=0.0001]和缓解期患者[(2.19±2.49)拷贝/1 000 PBMCs和(6.30±7.13)拷贝/1 000 CD3+细胞,P=0.0000和P=0.0005].各亚型ANLL患者的TREC水平差异无统计学意义(P＞0.05).结论 绝大多数ANLL型患者胸腺近期输出naive T细胞功能明显降低,缓解期患者的胸腺近期输出功能有一定程度恢复。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129428350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Awareness and monitoring during operation 操作过程中的意识和监控
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.002
岳云
{"title":"Awareness and monitoring during operation","authors":"岳云","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.002","url":null,"abstract":"认知(cognition)科学是在心理科学、信息科学、神经科学、科学语言学、比较人类学以及其它基础科学交叉后涌现出来的高度跨学科的新兴科学.认知科学是研究人类感知和人脑信息处理过程的科学.认知过程包括外在物理世界如何产生知觉,知觉信息如何输入、如何表达,乃至问题的求解.认知科学的研究范围包括知觉、注意、记忆、动作、语言、推理、思考、意识乃至情感在内的各个层面的认知活动。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128162173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study on the action of apoptosis in hysteromyoma damage by radio-frequencyablation 细胞凋亡在子宫肌瘤射频消融损伤中的作用研究
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.012
全晓广, 张晓薇
{"title":"Study on the action of apoptosis in hysteromyoma damage by radio-frequencyablation","authors":"全晓广, 张晓薇","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.012","url":null,"abstract":"目的 研究细胞凋亡在子宫肌瘤组织射频治疗中的作用.方法 光镜和电镜检测多电极射频消融的子宫肌瘤组织及未消融的子宫肌瘤组织中的凋亡细胞.用免疫组化二步法检测20例多电极射频消融的子宫肌瘤组织及未消融的子宫肌瘤组织中Bcl-2(B cell lymhpoma/leukemia-2)和Bax(Bcl-2 associated x-protein)蛋白的表达.结果 在消融灶边缘及其外0.5 cm的子宫肌瘤组织,细胞凋亡指数(16.3±3.4)高于未消融的子宫肌瘤组织(7.1±1.6,P＜0.01);同时Bcl-2蛋白表达减弱(P＜0.05),Bax蛋白表达增强(P＜0.05).结论 高温诱导的细胞凋亡在射频消融致子宫肌瘤治疗中起着相关作用,可能是消融引起肌瘤坏死的机制之一。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132174004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of different concentrations of sufentanil combined with propofol by target controlled infusion on anesthesia depth and measurement of EC50 and BIS50 不同浓度舒芬太尼联合异丙酚靶控输注对麻醉深度及EC50、BIS50测定的影响
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.006
韩光炜, 佘守章, 邬子林
{"title":"Influence of different concentrations of sufentanil combined with propofol by target controlled infusion on anesthesia depth and measurement of EC50 and BIS50","authors":"韩光炜, 佘守章, 邬子林","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.006","url":null,"abstract":"目的 观察不同浓度舒芬太尼联合丙泊酚靶控输注(TCI)对脑电双频谱指数(BIS)的影响,测定意识丧失及体动反应消失时相应丙泊酚效应室浓度(EC50)和BIS50的值.方法 择期全麻手术女性45例(ASA Ⅰ～Ⅱ级),病例来自广州医学院附属市一人民医院,随机分为A、B、C 3组,每组15例,分别以舒芬太尼0、0.12、0.24 μg/L作持续靶控输注,6 min后3组皆以0.6 mg/L启动丙泊酚靶控,并按0.3 mg/L递增丙泊酚.记录BIS基础值、舒芬太尼达效应室浓度后的BIS值、丙泊酚递增后的BIS和警觉镇静评估法(OAA/S)评分及挤压三角肌反应;丙泊酚效应室浓度为0.9、1.5、2.1 mg/L时,挤压三角肌后BIS的增加量;丙泊酚注射痛情况;测定意识丧失及体动反应消失时相应的EC50、EC95、BIS50、BIS95. 结果 B、C组BIS值在舒芬太尼输注前后变化差异无统计学意义(P＞0.05).意识丧失时A、B、C组的丙泊酚EC50/EC95分别为1.9/2.4、1.6/2.1、1.4/1.9 mg/L;BIS50/BIS95分别为64/55、69/61、72/59.体动反应消失时A、B、C组的丙泊酚EC50/EC95分别为3.0/3.7、1.4/2.0、0.9/1.4 mg/L;BIS50/BIS95分别为49/40、74/62、84/75.丙泊酚EC为0.9、1.5、2.1 mg/L时,三角肌挤压刺激的BIS增加量,A组与B、C组相比差异有统计学意义(P＜0.01).丙泊酚注射痛发生率A组明显高于C组(P＜0.05).结论 舒芬太尼0.12 μg/L、0.24 μg/L靶控不会引起BIS值显著变化,而与丙泊酚联合使用时可降低意识丧失、体动反应消失时的丙泊酚EC50,同时相应BIS50有所上升;且舒芬太尼可以明显降低刺激所导致的BIS增加量,减少丙泊酚注射痛现象。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115552031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effects of different inhalational induction concentration with sevoflurane on the depth of sedation and anesthesia of patients 七氟醚不同吸入诱导浓度对患者镇静麻醉深度的影响
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.007
程傲冰, 佘守章, 王淼, 许立新, 刘继云, 闫焱, 索琨
{"title":"The effects of different inhalational induction concentration with sevoflurane on the depth of sedation and anesthesia of patients","authors":"程傲冰, 佘守章, 王淼, 许立新, 刘继云, 闫焱, 索琨","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.007","url":null,"abstract":"目的 研究吸入不同浓度七氟醚全麻诱导对患者镇静程度和麻醉深度的影响.方法 选择本院60例择期行妇科腹腔镜手术的患者(ASAⅠ-Ⅱ级),采用随机双盲对照方法,研究比较3组患者吸入七氟醚4%(A组)、6%(B组)和8%(C组)浓度的诱导时间、诱导质量、镇静程度和麻醉深度的差异.并记录睫毛反射消失时间、患者入睡时间、不良事件发生率,同时记录患者的平均动脉压(MAP)、心率(HR)、脑电双频谱指数(BIS)、听觉诱发电位指数(AAI)和警觉与镇静评分(OAA/S评分).结果 C组睫毛反射消失时间为(45.5±4.4) s要明显短于A组(78.1±8.3) s和B组(55.2±6.9) s(P＜0.05),患者入睡时间C组(93.0±10.7) s也明显快于A组(148.4±13.8) s和B组(102.0±8.9) s(P＜0.05).OAA/S评分为3分时,C组的BIS值为 (74.1 ±3.8),AAI值为(37.2±4.2) ; C组诱导前后MAP下降和HR增快,且部分患者波动较大(P＜0.05).C组呼吸暂停发生率(30%)要高于A组和B组(P＜0.05).结论 单纯吸入七氟醚诱导患者入睡快,临床推荐吸入浓度为6%,其可提供良好的麻醉质量,且血流动力学平稳,不良反应小。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"133 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132785664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
转基因食品定性定量的检测分析方法 转基因食品定性定量的检测分析方法
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.023
李文立, 黄俊明
{"title":"转基因食品定性定量的检测分析方法","authors":"李文立, 黄俊明","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.023","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.023","url":null,"abstract":"转基因食品(genetically modified foods,GMF)是指由细胞DNA中经非生殖方法插入了特定外源基因或基因片段,并获得某种良好性状的动物、植物或微生物制成的食品.自1993年转基因作物Flavr Savr延熟番茄实现商品化以来,转基因食品的安全性问题一直为公众所注目,且至今尚无统一定论。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115977772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction of recombinant adenovirus of monocyte chemoattractant protein-1 by two-step homologous recombination in bacteria 两步同源重组法构建单核细胞趋化蛋白-1重组腺病毒
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.018
汪普宁, 彭和平, 刘启才
{"title":"Construction of recombinant adenovirus of monocyte chemoattractant protein-1 by two-step homologous recombination in bacteria","authors":"汪普宁, 彭和平, 刘启才","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.018","url":null,"abstract":"目的 两步法提高细菌内同源重组效率制备重组人单核细胞趋化蛋白1(MCP-1)复制缺陷型腺病毒.方法 制备含有腺病毒骨架质粒pAdEasy-1的大肠杆菌BJ5183,Hind Ⅲ酶切筛选未发生细菌内重组的菌落,并将其制备成感受态(BJ5183pAdEasy-1).RT-PCR法克隆得到MCP-1 cDNA片段,连接到pMD18-T载体后亚克隆至腺病毒穿梭载体pAdTrack上构建重组穿梭载体pAdTrack-MCP-1.PmeⅠ酶切线性化并碱性磷酸酶处理pAdTrack-MCP-1,然后电穿孔转化感受态BJ5183pAdEasy-1.PacⅠ酶切筛选得到正确重组的腺病毒载体pAd-MCP-1,将pAd-MCP-1转染入HEK293细胞中包装病毒颗粒,以PCR法鉴定.结果 成功构建pAd-MCP-1,效率可达50%以上,pAd-MCP-1能有效转染HEK293细胞并在细胞内包装.PCR鉴定病毒携带有MCP-1基因片段.结论 两步法细菌内同源重组构建重组腺病毒载体较常规电穿孔共转化效率高.成功制备重组腺病毒颗粒为进一步研究MCP-1的作用提供了重要的方法。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134061658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application value of direct digital radiography in lumber spine 直接数字x线摄影在腰椎中的应用价值
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-08-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.04.014
何建勋, 李新春, 孙翀鹏, 钟志伟
{"title":"Application value of direct digital radiography in lumber spine","authors":"何建勋, 李新春, 孙翀鹏, 钟志伟","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.04.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.04.014","url":null,"abstract":"目的 探讨直接数字化X线摄影( direct digitized radiography, DDR)在腰椎侧位影像中的应用价值.方法 随机抽取81例患者的DDR腰椎侧位影像,使用图像后处理方法中多级图像对比增益法,又称\"交响乐\"功能(multi-scale image contrast amplification, MUSICA)进行后处理,同时取另81例患者进行普通X线摄影,由放射科经验丰富的医生、技师各2名对所有的腰椎侧位影像进行分析,采用常规影像质量评价指标评价两组的影像质量.结果 (1)DDR腰椎侧位影像质量影像评分为13.00,普通X线影像评分为8.96,经非参数检验的配对符号秩和检验,依赖负秩计算的统计量(Z＝-7.88,P＜0.01),两组差异有统计学意义,DDR腰椎侧位组明显优于普通平片组;(2)81例中DDR腰椎图像显示满意率为92.6%(75/81), 普通X线平片显示满意率为61.7%(50/81) ,两者差异有统计学意义(χ2 = 21.89, P＜0.01). 结论 与普通X线影像相比,DDR影像能更好的显示下部腰椎椎体、附件及周围软组织,可获得良好的图像,有利于放射诊断工作。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126482257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The impact of anal electric stimulation on acclimation and tension of dog rectum 肛门电刺激对犬直肠适应和张力的影响
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.011
聂玉强, 姜英杰, 李瑜元, 陈建德
{"title":"The impact of anal electric stimulation on acclimation and tension of dog rectum","authors":"聂玉强, 姜英杰, 李瑜元, 陈建德","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.011","url":null,"abstract":"目的 研究低频长脉冲肛门电刺激(AES)括约肌对狗直肠顺应性、张力和直肠肛门抑制反射(RAIF)的影响.方法 9只健康狗参加3次实验研究.肛门括约肌压力(ASP)以每秒收缩曲线下面积来表达.直肠张力是在压力控制模型下测定.直肠的顺应性在以压力渐增的相性气囊扩张模型下测定.AES是通过安装在测压管上的一对环状电极进行.结果 AES期间的RAIF最低阈容量[(27.5±0.9) ml]显著高于无AES的对照期[(22.5±1.9) ml](P＜0.05).用45 ml气体扩张气球时,AES期和对照期的ASP下降百分比在两者间差异无统计学意义.与基础对照相比,AES显著降低P1/2值[(11.1±1.5)比(16.7±1.1), P＜0.05] 和κ值 [(11.6±2.5)比 (20.5±2.6) mm Hg, P=0.0095],表示AES增高了直肠的顺应性,但对直肠张力无明显改变.结论 肛门内应用低频长脉冲电刺激可使健康狗的ASP增加.AES能增加直肠的顺应性,但不改变直肠的张力,且RAIF仍完全保留。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117056702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of NPY on Ca2+/CaM-dependent calcineurin signaling pathway of cardiomyocytes in rat NPY对大鼠心肌细胞Ca2+/ cam依赖性钙调磷酸酶信号通路的影响
Journal of Modern Clinical Medical Bioengineering Pub Date : 2006-06-15 DOI: 10.3760/CMA.J.ISSN.1008-634X.2006.03.010
李晓云, 陈敏生, 黄少华, 董颀, 李荧辉, 张舒
{"title":"Effect of NPY on Ca2+/CaM-dependent calcineurin signaling pathway of cardiomyocytes in rat","authors":"李晓云, 陈敏生, 黄少华, 董颀, 李荧辉, 张舒","doi":"10.3760/CMA.J.ISSN.1008-634X.2006.03.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-634X.2006.03.010","url":null,"abstract":"目的 观察神经肽 Y (NPY)对心肌细胞内Ca2+及钙调素(CaM)依赖的钙调神经磷酸酶(CaN)信号传导途径的影响.方法 以NPY刺激心肌细胞.用环胞素A(CsA)特异性抑制CaN活性.应用Western印迹测细胞内CaN-α蛋白表达.采用组织化学法测CaN酶活性.用Fluo3-AM荧光标记法观察心肌细胞胞质及核内钙离子浓度变化.结果 加入100 nmol/L NPY刺激后,心肌细胞CaN酶活力较对照组明显增高(P＜0.05),10 nmol/L NPY组和CsA 组(100 nmol/L NPY + 5 μg/ml CsA) CaN酶活性与对照组相比,差异无统计学意义.100 nmol/L NPY组心肌细胞内CaN-α蛋白表达较对照组显著增高(P＜0.05). 加入100 nmol/L NPY在10 min内,心肌细胞胞浆及核内钙含量与对照组相比,差异无统计学意义.经100 nmol/L NPY刺激24 h后,心肌细胞胞浆及核内钙含量明显高于对照组(P均＜0.01).结论 NPY可活化心肌细胞内Ca2+及CaM-CaN途径.NPY刺激下产生的细胞内钙增加,可能是其活化CaN途径的始动环节。","PeriodicalId":189947,"journal":{"name":"Journal of Modern Clinical Medical Bioengineering","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123262091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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