Nature Microbiology最新文献_第4页

AP2-HS and a GDV1 regulatory feedback loop mediate environmental induction of sexual conversion in Plasmodium falciparum. AP2-HS和GDV1调节反馈回路介导恶性疟原虫性转化的环境诱导。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-17 DOI: 10.1038/s41564-026-02473-6

Elisabet Tintó-Font, Núria Casas-Vila, César Martínez-Guardiola, Alba Pérez-Cantero, Neus Ràfols, Prince B Nyarko, Alfred Cortés

{"title":"AP2-HS and a GDV1 regulatory feedback loop mediate environmental induction of sexual conversion in Plasmodium falciparum.","authors":"Elisabet Tintó-Font, Núria Casas-Vila, César Martínez-Guardiola, Alba Pérez-Cantero, Neus Ràfols, Prince B Nyarko, Alfred Cortés","doi":"10.1038/s41564-026-02473-6","DOIUrl":"https://doi.org/10.1038/s41564-026-02473-6","url":null,"abstract":"Human-to-mosquito transmission of the malaria parasite Plasmodium falciparum requires conversion of some parasites to gametocytes, a non-replicative sexual form. Specific conditions of the human blood environment that induce parasite stress increase sexual conversion rates. Sexual conversion is triggered by the transcription factor AP2-G and its upstream activator, GDV1. However, the molecular mechanisms underlying environmentally induced sexual conversion are unclear. Here we show that environmental induction of gdv1 expression and sexual conversion requires the transcription factor AP2-HS, which plays a dual role as activator or repressor in different pathways. Activation of gdv1 expression, either spontaneously or in response to environmental conditions, triggers a positive-negative regulatory feedback loop between GDV1 and its antisense long non-coding RNA (gdv1-as) repressor. This involves GDV1-dependent displacement of heterochromatin from the gdv1-as promoter. Our results describe a complex regulatory mechanism enabling P. falciparum to adjust its investment in transmission under different stress conditions.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":18.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional capacities drive recruitment of bacteria into plant root microbiota. 功能能力驱动细菌进入植物根系微生物群的招募。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-17 DOI: 10.1038/s41564-026-02493-2

Gijs Selten, Florian Lamouche, Adrián Gómez-Repollés, José Luiz López, Xiao-Mei Zhang, Caitlan Smart, Zuzana Blahovska, Geovanna Zarate Camargo, Simon Kelly, Ronnie de Jonge, Simona Radutoiu

{"title":"Functional capacities drive recruitment of bacteria into plant root microbiota.","authors":"Gijs Selten, Florian Lamouche, Adrián Gómez-Repollés, José Luiz López, Xiao-Mei Zhang, Caitlan Smart, Zuzana Blahovska, Geovanna Zarate Camargo, Simon Kelly, Ronnie de Jonge, Simona Radutoiu","doi":"10.1038/s41564-026-02493-2","DOIUrl":"10.1038/s41564-026-02493-2","url":null,"abstract":"Root-associated microbiomes are shaped by the plant, yet vary across environments and hosts, challenging prediction and engineering. Here, to uncover principles of bacterial selection at the root-soil interface, we applied a systems-level approach using reconstitution studies with communities of isolates from Arabidopsis, barley and Lotus grown in soil. Functional divergence among the microbiota of the host plants reflected distinct strategies: in Arabidopsis and barley, recruitment was primarily shaped by inoculum, while Lotus root environment favoured fewer, functionally diverse isolates, akin to a 'Swiss army knife' strategy. Despite taxonomic variability, root microbiomes encoded overlapping functions. Across major taxa, isolates with broad but distinct functional repertoires within their families were consistently more abundant. Using a genome-to-function framework that is function centric, taxonomically inclusive and host-context aware, we identified 266 functions enriched across all root microbiomes. This functional backbone emerged as a core signature of plant-associated bacteria, providing a solid foundation for microbiome engineering in agriculture.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":18.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial growth rate in populations and single cells 细菌在群体和单细胞中的生长速度。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-17 DOI: 10.1038/s41564-026-02449-6

Petra Anne Levin, Jue D. Wang

{"title":"Bacterial growth rate in populations and single cells","authors":"Petra Anne Levin, Jue D. Wang","doi":"10.1038/s41564-026-02449-6","DOIUrl":"10.1038/s41564-026-02449-6","url":null,"abstract":"Bacterial growth is straightforward to measure, complex in origin and exquisitely sensitive to environmental and genetic perturbations. As a quantifiable parameter, growth rate is central to modern microbiology, conflictingly portrayed as both an output and an input of physiological state. Here we provide a short history of bacterial growth measurement and explain the centrality of growth rate to modern microbiology. We then examine the physical and regulatory connections between nutrient availability, macromolecular synthesis and growth rate. We focus particular attention on (p)ppGpp, a nucleotide alarmone that offers the best-characterized mechanism for coordinating environmental conditions with growth. We end by addressing the cell-autonomous nature of homeostatic control as the basis of growth rate heterogeneity, discussing why the individual cell is paramount when it comes to understanding the relationship between different aspects of physiology, including macromolecular composition, cell size, cell cycle progression and growth rate. In this Perspective, Levin and Wang give a brief history of bacterial growth rate and discuss how it is connected to nutrient availability and macromolecule synthesis, with a focus on (p)ppGpp as a central hub regulating resource allocation and, indirectly, growth rate at the single-cell level.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"11 10","pages":"2685-2693"},"PeriodicalIF":18.7,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aminopeptidase N is a receptor for hedgehog merbecoviruses 氨基肽酶N是刺猬merbeco病毒的受体。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-16 DOI: 10.1038/s41564-026-02447-8

Min Jin, Victoria A. Jefferson, Zhe Zhao, Nicholas J. Catanzaro, Luca De Sabato, Emma L. Keeler, Bridget L. Menasche, Cameron Hoffman, Boyd L. Yount, Anna Castelli, Ilaria Di Bartolo, Gabriele Vaccari, Tyler N. Starr, Stephanie N. Seifert, Adrian A. Castellanos, Barbara A. Han, Leonid Serebryannyy, Alicen B. Spaulding, Daniel C. Douek, Ana Moreno, Ralph S. Baric, Craig B. Wilen, James M. Rini, Michael Letko

{"title":"Aminopeptidase N is a receptor for hedgehog merbecoviruses","authors":"Min Jin, Victoria A. Jefferson, Zhe Zhao, Nicholas J. Catanzaro, Luca De Sabato, Emma L. Keeler, Bridget L. Menasche, Cameron Hoffman, Boyd L. Yount, Anna Castelli, Ilaria Di Bartolo, Gabriele Vaccari, Tyler N. Starr, Stephanie N. Seifert, Adrian A. Castellanos, Barbara A. Han, Leonid Serebryannyy, Alicen B. Spaulding, Daniel C. Douek, Ana Moreno, Ralph S. Baric, Craig B. Wilen, James M. Rini, Michael Letko","doi":"10.1038/s41564-026-02447-8","DOIUrl":"10.1038/s41564-026-02447-8","url":null,"abstract":"Merbecoviruses, closely related to the Middle East respiratory syndrome coronavirus, circulate in hedgehogs throughout Europe and Asia, raising concerns about zoonotic transmission to humans and domestic animals. However, it is not clear how these viruses enter host cells. Here we tested three coronavirus receptors from European hedgehogs (Erinaceus europaeus) in cell-based assays and identified aminopeptidase N (APN) as a receptor for hedgehog merbecoviruses. We verified it by pseudotype experiments using an in vitro reporter system based on replication competent vesicular stomatitis virus and protein binding assays. Hedgehog coronavirus spike proteins showed enhanced infectivity when produced at 33 °C, approximating the physiological hedgehog body temperature, as compared with 37 °C. A screen of 30 mammalian APN orthologues showed restricted cross-species receptor use, including the inability to use human APN. Electron cryomicroscopy revealed a distinct glycoprotein–receptor interface unlike known coronavirus spike–APN interactions, clarifying species barriers. These findings broaden our understanding of receptor use across merbecoviruses and betacoronaviruses and inform risk assessments for viral emergence. Hedgehog merbecoviruses utilize hedgehog aminopeptidase N, but not the human orthologue, as their entry receptor. Structural resolution of the glycoprotein–receptor interface reveals the molecular interactions underlying this species-specific barrier.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"11 10","pages":"2839-2852"},"PeriodicalIF":18.7,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41564-026-02447-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent use of aminopeptidase N receptor by hedgehog merbecoviruses 刺猬merbeco病毒对氨基肽酶N受体的聚合利用。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-16 DOI: 10.1038/s41564-026-02478-1

Peng Liu, Qianhui Zhu, Jingyi Liu, Zhixing Ma, Guocui Mou, Yuanling Yu, Chen Liu, Chengbao Ma, Junyu Si, Xiao Yang, Meiling Huang, Yanchu Liu, Yehui Sun, Xiao Yu, Yucheng Sun, Tianci Liu, Lingling Yu, Qianran Wang, Honghao Ma, Qi Lv, Jing Li, Linlin Bao, Yunlong Cao, Xiangxi Wang, Huan Yan

{"title":"Convergent use of aminopeptidase N receptor by hedgehog merbecoviruses","authors":"Peng Liu, Qianhui Zhu, Jingyi Liu, Zhixing Ma, Guocui Mou, Yuanling Yu, Chen Liu, Chengbao Ma, Junyu Si, Xiao Yang, Meiling Huang, Yanchu Liu, Yehui Sun, Xiao Yu, Yucheng Sun, Tianci Liu, Lingling Yu, Qianran Wang, Honghao Ma, Qi Lv, Jing Li, Linlin Bao, Yunlong Cao, Xiangxi Wang, Huan Yan","doi":"10.1038/s41564-026-02478-1","DOIUrl":"10.1038/s41564-026-02478-1","url":null,"abstract":"Coronaviruses circulating in wildlife, such as European and Asian hedgehog merbecoviruses (EriCoVs), pose a potential transmission risk. Knowledge of viral receptors can inform risk assessment and development of countermeasures; however, the functional receptor for EriCoVs is unknown. Here we identify hedgehog aminopeptidase N (APN) as a functional receptor for various EriCoVs. Analysis of APN orthologues from 93 species reveals a restricted APN tropism for EriCoVs, primarily utilizing APN from hedgehogs and to a lesser extent felid APN. Cryogenic electron microscopy analysis of the EriCoV–APN complex reveals an APN-binding mode that is distinct from those used by alpha- and deltacoronaviruses. Functional assays suggest that proteolytic activation of EriCoV spikes can be facilitated by multiple proteases in a context- and strain-dependent manner. Neutralizing antibodies targeting the EriCoV receptor-binding domain and hedgehog APN effectively blocked EriCoV pseudovirus entry or amplification. Receptor-binding-domain-targeting antibodies were further elucidated by cryogenic electron microscopy analysis. Together, these findings can inform assessment of merbecovirus spillover and pathogenesis. Hedgehog merbecoviruses are shown to use aminopeptidase N as a functional receptor through a distinct binding mode, revealing convergent receptor usage among merbecoviruses.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"11 10","pages":"2821-2838"},"PeriodicalIF":18.7,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41564-026-02478-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophage-encoding engineered bacteria enable prophylactic lytic phage therapy for enteric infection in mice. 原噬菌体编码工程细菌可用于小鼠肠道感染的预防性裂解噬菌体治疗。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-16 DOI: 10.1038/s41564-026-02484-3

Rogerio A Bataglioli, Hiba Baaziz, Hallie F Avalos, Lindsay Smith, Zachary R Baker, Rita J Makhlouf, David da Silva Barreira, Harsimran Kaur, Teresa Southard, Bryan B Hsu

{"title":"Prophage-encoding engineered bacteria enable prophylactic lytic phage therapy for enteric infection in mice.","authors":"Rogerio A Bataglioli, Hiba Baaziz, Hallie F Avalos, Lindsay Smith, Zachary R Baker, Rita J Makhlouf, David da Silva Barreira, Harsimran Kaur, Teresa Southard, Bryan B Hsu","doi":"10.1038/s41564-026-02484-3","DOIUrl":"https://doi.org/10.1038/s41564-026-02484-3","url":null,"abstract":"Phage therapy is a promising antibacterial approach but it has limited efficacy against enteric bacterial infections, such as Salmonella enterica Typhimurium (STm). Here we hypothesized that establishing high concentrations of a pathogen-targeting phage in the gut before infection would prophylactically protect against future infection. To this end, we engineered non-pathogenic Escherichia coli to encode a prophage that produces obligately lytic phage progeny targeting STm while overcoming its host defence systems, including restriction-modification systems. We show that prophylactic gut colonization with this lytic phage-producing lysogen of E. coli produces high levels of lytic phage before infection, inhibits the establishment of STm after infection and improves survival in mouse models of STm-induced colitis. Using a mutant STm strain limited to intestinal colonization, we show that phage-producing E. coli consistently colonized, produced high phage levels and eliminated STm from the intestinal tract. Our findings present a proof-of-concept that prophylactic prophage therapy can protect against an enteric infection.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":18.7,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148983307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-EM structures of Candida albicans chitin synthase Chs1 reveal a druggable translocation channel. 白色念珠菌几丁质合成酶Chs1的低温电镜结构揭示了一个可用药的易位通道。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-15 DOI: 10.1038/s41564-026-02485-2

Zhenning Ren, Abhishek Chhetri, Chang Liu, Ziqiang Guan, ShuYu Offner, Dmitry Kozhanov, Mario J Borgnia, Wonpil Im, Kenichi Yokoyama, Seok-Yong Lee

{"title":"Cryo-EM structures of Candida albicans chitin synthase Chs1 reveal a druggable translocation channel.","authors":"Zhenning Ren, Abhishek Chhetri, Chang Liu, Ziqiang Guan, ShuYu Offner, Dmitry Kozhanov, Mario J Borgnia, Wonpil Im, Kenichi Yokoyama, Seok-Yong Lee","doi":"10.1038/s41564-026-02485-2","DOIUrl":"10.1038/s41564-026-02485-2","url":null,"abstract":"Invasive candidiasis is a leading cause of hospital-acquired bloodstream infections with high mortality. While the fungal cell wall is an excellent therapeutic target, inhibitor development against the essential chitin synthase (Chs) has been hampered by the absence of structural and mechanistic understanding of class II Chs, which contribute to fungal viability. Here we present cryo-electron microscopy structures of Candida albicans class II Chs (CaChs1) at 2.93-3.38 Å resolution, providing insights into its mechanisms of early elongation, chito-oligomer translocation and inhibition by the CaChs1-specific non-competitive inhibitor diynyl arylamine (DA). Chitin elongation and translocation are coupled to coordinated motion of the glycosyltransferase domain and the dimer interface. Notably, DA binds within the chitin translocation channel where a regulatory lipid resides and inhibits the enzyme by occluding product polymer extrusion. Importantly, DA showed potent synergy with the class I Chs inhibitor nikkomycin Z against C. albicans and Candida auris. These findings establish the chitin translocation channel as a druggable site for rational antifungal design.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":18.7,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13648194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ambient temperature is encoded in the metagenome of microbial communities 环境温度被编码在微生物群落的宏基因组中。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-15 DOI: 10.1038/s41564-026-02479-0

引用次数: 0

Histone H2B monoubiquitination drives sexual commitment in malaria parasites 组蛋白H2B单泛素化驱动疟疾寄生虫的性行为。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-15 DOI: 10.1038/s41564-026-02475-4

Zhiwei Jiao, Lirong Lin, Ruoyu Tang, Chuan-qi Zhong, Chengyi Wu, Weiqi Li, Ruixue Xu, Yaqi Gao, Pan He, Changhong Wang, Qiong-qiong Zhang, Shengfa Liu, Jing Yuan, Qingfeng Zhang, Jian Li

{"title":"Histone H2B monoubiquitination drives sexual commitment in malaria parasites","authors":"Zhiwei Jiao, Lirong Lin, Ruoyu Tang, Chuan-qi Zhong, Chengyi Wu, Weiqi Li, Ruixue Xu, Yaqi Gao, Pan He, Changhong Wang, Qiong-qiong Zhang, Shengfa Liu, Jing Yuan, Qingfeng Zhang, Jian Li","doi":"10.1038/s41564-026-02475-4","DOIUrl":"10.1038/s41564-026-02475-4","url":null,"abstract":"Transmission of malaria parasites to mosquito vectors relies on the successful conversion from asexual blood-stage forms into sexual gametocytes. Protein ubiquitination regulates many cellular processes, including cell differentiation in other eukaryotes, but whether specific ubiquitination machinery mediates Plasmodium sexual conversion is unclear. Here we conduct CRISPR–Cas9 mutagenesis screens to systematically profile Plasmodium yoelii E2 ubiquitin-conjugating enzymes for their impact on parasite development. We demonstrate that an E2 enzyme (Rad6B), and its partner RING-type E3 ligase (Tex1), play determinant roles in gametocytogenesis and transmission to mosquitoes. Deletion of either Rad6B or Tex1 results in severe defects in gametocyte production and oocyst formation. The Rad6B–Tex1 complex catalyses histone H2B monoubiquitination in Plasmodium yoelii and Plasmodium falciparum. H2B monoubiquitination promotes H3K4 tri-methylation occupancy at the promoter region of the gene encoding transcription factor AP2-G, a master regulator of sexual conversion, thereby inducing ap2-g expression and initiating sexual commitment. These findings reveal a key epigenetic mechanism leading to sexual differentiation in both human and rodent malaria parasites, providing potential targets for transmission-blocking interventions. The Rad6B–Tex1 ubiquitination complex drives malaria parasite sexual commitment by monoubiquitinating histone H2B, and thereby enabling gametocyte formation and mosquito transmission.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":"11 10","pages":"2805-2820"},"PeriodicalIF":18.7,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of direct methane production from long-chain fatty acids by thermophilic Archaeoglobi. 嗜热古红藻从长链脂肪酸直接产生甲烷的证据。

IF 18.7 1区生物学

Nature Microbiology Pub Date : 2026-09-15 DOI: 10.1038/s41564-026-02490-5

Tiantian Yu, Shujian Yuan, Yinzhao Wang, Fengping Wang

{"title":"Evidence of direct methane production from long-chain fatty acids by thermophilic Archaeoglobi.","authors":"Tiantian Yu, Shujian Yuan, Yinzhao Wang, Fengping Wang","doi":"10.1038/s41564-026-02490-5","DOIUrl":"https://doi.org/10.1038/s41564-026-02490-5","url":null,"abstract":"Methanogenic degradation of long-chain fatty acids has traditionally been thought to occur through syntrophic partnerships between fatty acid-degrading bacteria and methanogenic archaea. However, recent genomic evidence suggests that certain archaea may independently carry out the entire process. Here we report the enrichment of an archaeon from the class Archaeoglobi, Candidatus Methanoglobus sphaerolipidus DLY3, from hot spring sediments in Tengchong, China. The results of selective enrichment, growth experiments, microscopy, stable isotope tracing, metagenomics and metatranscriptomics suggest that Ca. M. sphaerolipidus directly converts long-chain fatty acids to methane. This transformation involves the beta-oxidation pathway, the Wood-Ljungdahl pathway and methanogenic methyl-coenzyme M reductase and methyltransferase complexes-a process we term liparotrophy. In addition to oleic acid, Ca. M. sphaerolipidus is also capable of utilizing methanol as a substrate for methanogenesis. Our findings expand the known substrate range for methanogenic archaea beyond carbon dioxide reduction, acetoclastic methanogenesis, methylotrophy, methyl reduction, methoxydotrophy and the recently reported alkylotrophy.","PeriodicalId":18992,"journal":{"name":"Nature Microbiology","volume":" ","pages":""},"PeriodicalIF":18.7,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0