Molecular Cell最新文献_第5页

Molecular impact of mutations in RNA splicing factors in cancer RNA 剪接因子突变对癌症的分子影响

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-14 DOI: 10.1016/j.molcel.2024.07.019

引用次数: 0

Functional and structural insights into RAS effector proteins 对 RAS 效应蛋白功能和结构的深入研究

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-13 DOI: 10.1016/j.molcel.2024.07.024

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OPA1 promotes ferroptosis by augmenting mitochondrial ROS and suppressing an integrated stress response OPA1 通过增加线粒体 ROS 和抑制综合应激反应促进铁中毒

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-13 DOI: 10.1016/j.molcel.2024.07.020

{"title":"OPA1 promotes ferroptosis by augmenting mitochondrial ROS and suppressing an integrated stress response","authors":"","doi":"10.1016/j.molcel.2024.07.020","DOIUrl":"https://doi.org/10.1016/j.molcel.2024.07.020","url":null,"abstract":"Ferroptosis, an iron-dependent form of nonapoptotic cell death mediated by lipid peroxidation, has been implicated in the pathogenesis of multiple diseases. Subcellular organelles play pivotal roles in the regulation of ferroptosis, but the mechanisms underlying the contributions of the mitochondria remain poorly defined. Optic atrophy 1 (OPA1) is a mitochondrial dynamin-like GTPase that controls mitochondrial morphogenesis, fusion, and energetics. Here, we report that human and mouse cells lacking OPA1 are markedly resistant to ferroptosis. Reconstitution with OPA1 mutants demonstrates that ferroptosis sensitization requires the GTPase activity but is independent of OPA1-mediated mitochondrial fusion. Mechanistically, OPA1 confers susceptibility to ferroptosis by maintaining mitochondrial homeostasis and function, which contributes both to the generation of mitochondrial lipid reactive oxygen species (ROS) and suppression of an ATF4-mediated integrated stress response. Together, these results identify an OPA1-controlled mitochondrial axis of ferroptosis regulation and provide mechanistic insights for therapeutically manipulating this form of cell death in diseases.","PeriodicalId":18950,"journal":{"name":"Molecular Cell","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HLTF resolves G4s and promotes G4-induced replication fork slowing to maintain genome stability HLTF 可解决 G4 问题，并促进 G4 诱导的复制叉减速，以维持基因组稳定性

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-13 DOI: 10.1016/j.molcel.2024.07.018

引用次数: 0

Nuclear retention coupled with sequential polyadenylation dictates post-transcriptional m6A modification in the nucleus 核保留与连续多腺苷酸化决定了转录后 m6A 在细胞核中的修饰

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-09 DOI: 10.1016/j.molcel.2024.07.017

{"title":"Nuclear retention coupled with sequential polyadenylation dictates post-transcriptional m6A modification in the nucleus","authors":"","doi":"10.1016/j.molcel.2024.07.017","DOIUrl":"https://doi.org/10.1016/j.molcel.2024.07.017","url":null,"abstract":"N6-methyladenosine (m6A) modification is deemed to be co-transcriptionally installed on pre-mRNAs, thereby influencing various downstream RNA metabolism events. However, the causal relationship between m6A modification and RNA processing is often unclear, resulting in premature or even misleading generalizations on the function of m6A modification. Here, we develop 4sU-coupled m6A-level and isoform-characterization sequencing (4sU-m6A-LAIC-seq) and 4sU-GLORI to quantify the m6A levels for both newly synthesized and steady-state RNAs at transcript and single-base-resolution levels, respectively, which enable dissecting the relationship between m6A modification and alternative RNA polyadenylation. Unexpectedly, our results show that many m6A addition events occur post-transcriptionally, especially on transcripts with high m6A levels. Importantly, we find higher m6A levels on shorter 3′ UTR isoforms, which likely result from sequential polyadenylation of longer 3′ UTR isoforms with prolonged nuclear dwelling time. Therefore, m6A modification can also take place post-transcriptionally to intimately couple with other key RNA metabolism processes to establish and dynamically regulate epi-transcriptomics in mammalian cells.","PeriodicalId":18950,"journal":{"name":"Molecular Cell","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141909321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Puff, the polytene chromosome: Visualizing transcription elongation control 多腺染色体 "泡芙"：转录伸长控制的可视化

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-08 DOI: 10.1016/j.molcel.2024.07.014

引用次数: 0

Decoding the end of message: PASTA provides food for thought on mechanisms of alternative polyadenylation 解码信息末端：PASTA 为替代性多腺苷酸化机制提供了思路

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-08 DOI: 10.1016/j.molcel.2024.07.015

引用次数: 0

Two HUSH complexes connect a direct LINE to innate immunity 两个 HUSH 复合物与先天性免疫直接相连

IF 16 1区生物学

Molecular Cell Pub Date : 2024-08-08 DOI: 10.1016/j.molcel.2024.07.010