Genetics and molecular research : GMR最新文献_第9页

ΔF508 mutation screening of healthy individuals from two populations in Espírito Santo State, Brazil. ΔF508巴西圣州Espírito两个人群健康个体的突变筛查。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049387

A. Lanes, L. S. Louro, D. P. Ventorim, E. Stur, F. M. Garcia, L. P. Agostini, L. N. R. Alves, R. S. Reis, I. Louro, R. S. Dettogni

{"title":"ΔF508 mutation screening of healthy individuals from two populations in Espírito Santo State, Brazil.","authors":"A. Lanes, L. S. Louro, D. P. Ventorim, E. Stur, F. M. Garcia, L. P. Agostini, L. N. R. Alves, R. S. Reis, I. Louro, R. S. Dettogni","doi":"10.4238/gmr15049387","DOIUrl":"https://doi.org/10.4238/gmr15049387","url":null,"abstract":"The ΔF508 mutation is the most common cause of cystic fibrosis and its prevalence varies worldwide. For instance, up to 20-fold variations in its frequency have been recorded across different areas of Brazil. This study aimed to compare the distribution of ΔF508 among healthy individuals of admixed Portuguese descent from Espírito Santo (ES), a state in Southeastern Brazil, to that in a subpopulation of Pomeranian descent, considered to be an isolated group in which the European gene pool has been preserved, living in Santa Maria do Jetibá (also in ES). We found this mutation to be present at a frequency of 0.81% among the Pomeranian group, and 0% in the general ES population. No genetic differentiation was noted between the two populations examined (FST = 0.004), and these frequencies were found to be similar to those estimated in other states of Southeastern Brazil. Although the population of Santa Maria de Jetibá has retained Pomeranian traits, such as language, fair skin, and eye color, to date, there is no evidence of inbreeding in this group (FIS = -0.004). Screening healthy individuals for the ΔF508 mutation can facilitate genetic counseling for cystic fibrosis, as well as inform evolutionary and population studies.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120900175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of miR-27a upregulation on thyroid cancer cells migration, invasion, and angiogenesis. miR-27a上调对甲状腺癌细胞迁移、侵袭和血管生成的影响。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049070

Y. L. Wang, W. Gong, Q. Yuan

{"title":"Effects of miR-27a upregulation on thyroid cancer cells migration, invasion, and angiogenesis.","authors":"Y. L. Wang, W. Gong, Q. Yuan","doi":"10.4238/gmr15049070","DOIUrl":"https://doi.org/10.4238/gmr15049070","url":null,"abstract":"Thyroid cancer is the most common type of endocrine tumor. MicroRNAs (miRNAs) play a critical role in a variety of diseases, especially cancer occurrence and progression. However, the specific mechanism by which miRNAs trigger disease states has not been fully elucidated. This study aims to investigate the role of miR-27a in thyroid cancer cells. A wound healing assay was adopted to examine cell migration. A transwell assay was applied to assess cell invasion. A thyroid cancer xenograft model was established using BALB/c nude mice. Western blot was performed to quantify iNOS expression. Tumor tissue blood vessel density was evaluated via immunohistochemistry assays. The results indicated that miR-27a downregulation inhibited thyroid cancer cell migration, while upregulation of miR-27a promoted thyroid cancer cell migration (P < 0.05). Furthermore, reduction in miR-27a expression suppressed thyroid cancer cell invasion (P < 0.05). In the nude mouse model of thyroid cancer xenograft, upregulation of miR-27 induced iNOS expression in pathological tumor tissues, whereas miR-27a inhibition resulted in the opposite effect (P < 0.05). CD105 level was also significantly increased during miR-27a upregulation, and was declined when miR-27a was inhibited (P < 0.05). In conclusion, miR-27a upregulation in thyroid cancer cells affects tumor cell migration, invasion, and angiogenesis by targeting downstream genes. Therefore, miR27a may act as a biomarker of thyroid cancer.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133251008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Characterization and application of newly developed polymorphic microsatellite markers in the Ezo red fox (Vulpes vulpes schrencki). Ezo赤狐多态微卫星标记的鉴定与应用。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049104

T. Tada, Y. Seki, Y. Kameyama, Y. Kikkawa, K. Wada

引用次数: 1

Correlation between TRAIL and caspase-8 expression and their relationship with cell proliferation and apoptosis in human osteosarcoma. TRAIL与caspase-8在人骨肉瘤中表达的相关性及其与细胞增殖和凋亡的关系

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15048876

X. Ning, X. Shang, Y. Zhuang, M. Liu, Hua Yang, Hao Zhang, M. Huang

{"title":"Correlation between TRAIL and caspase-8 expression and their relationship with cell proliferation and apoptosis in human osteosarcoma.","authors":"X. Ning, X. Shang, Y. Zhuang, M. Liu, Hua Yang, Hao Zhang, M. Huang","doi":"10.4238/gmr15048876","DOIUrl":"https://doi.org/10.4238/gmr15048876","url":null,"abstract":"Osteosarcoma is a common malignant bone tumor that mainly affects children and adolescents. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor superfamily. Caspase-8 appears in the upstream of apoptosis signaling pathway among caspases. We investigated TRAIL and caspase-8 levels in osteosarcoma patients to determine their correlation with cell proliferation and apoptosis. Osteosarcoma and osteochondroma patients receiving surgery in our hospital were selected. TRAIL and caspase-8 expression levels in tissue were determined by immunohistochemistry, and protein levels in cells were evaluated by western blotting. Human osteosarcoma cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The osteosarcoma and osteochondroma cell cycles and apoptosis were investigated by flow cytometry. Correlation analysis was applied to TRAIL and caspase-8 levels during cell apoptosis. Positive TRAIL and caspase-8 expression rates in osteosarcoma tissue were significantly lower than in the controls (P < 0.05). TRAIL (0.114 ± 0.002) and caspase-8 (0.352 ± 0.124) levels in experimental cells were obviously lower than in the controls (P < 0.05). Osteosarcoma cells in the experimental group demonstrated higher proliferation and lower apoptosis at 24, 48, and 72 h (P < 0.05). The experimental cell number increased in the G1 stage and decreased in the S stage (P < 0.05). TRAIL and caspase-8 proteins showed positive correlation with apoptosis in osteosarcoma (P < 0.05). Human osteosarcoma presented reduced TRAIL and caspase-8 levels with enhanced cell proliferation and reduced apoptosis. TRAIL and caspase-8 expression levels were positively correlated with apoptosis in osteosarcoma.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"53 19","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113973905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular characterization and population structure study of cambuci: strategy for conservation and genetic improvement. cambuzi的分子特征和种群结构研究:保护和遗传改良策略。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049134

D. N. Santos, C. F. Nunes, T. Setotaw, R. Pio, M. Pasqual, G. Cançado

{"title":"Molecular characterization and population structure study of cambuci: strategy for conservation and genetic improvement.","authors":"D. N. Santos, C. F. Nunes, T. Setotaw, R. Pio, M. Pasqual, G. Cançado","doi":"10.4238/gmr15049134","DOIUrl":"https://doi.org/10.4238/gmr15049134","url":null,"abstract":"Cambuci (Campomanesia phaea) belongs to the Myrtaceae family and is native to the Atlantic Forest of Brazil. It has ecological and social appeal but is exposed to problems associated with environmental degradation and expansion of agricultural activities in the region. Comprehensive studies on this species are rare, making its conservation and genetic improvement difficult. Thus, it is important to develop research activities to understand the current situation of the species as well as to make recommendations for its conservation and use. This study was performed to characterize the cambuci accessions found in the germplasm bank of Coordenadoria de Assistência Técnica Integral using inter-simple sequence repeat markers, with the goal of understanding the plant's population structure. The results showed the existence of some level of genetic diversity among the cambuci accessions that could be exploited for the genetic improvement of the species. Principal coordinate analysis and discriminant analysis clustered the 80 accessions into three groups, whereas Bayesian model-based clustering analysis clustered them into two groups. The formation of two cluster groups and the high membership coefficients within the groups pointed out the importance of further collection to cover more areas and more genetic variability within the species. The study also showed the lack of conservation activities; therefore, more attention from the appropriate organizations is needed to plan and implement natural and ex situ conservation activities.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131881251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cryopreservation of Schisandra chinensis (Turcz.) Baill callus and subsequent plant regeneration. 五味子的低温保存球茎愈伤组织和随后的植株再生。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049342

D. Sun, Y-F Yu, H. Qin, P. Xu, Y. Zhao, Y.X. Liu, Z. Wang, S. Fan, Y.M. Yang, J. Ai

{"title":"Cryopreservation of Schisandra chinensis (Turcz.) Baill callus and subsequent plant regeneration.","authors":"D. Sun, Y-F Yu, H. Qin, P. Xu, Y. Zhao, Y.X. Liu, Z. Wang, S. Fan, Y.M. Yang, J. Ai","doi":"10.4238/gmr15049342","DOIUrl":"https://doi.org/10.4238/gmr15049342","url":null,"abstract":"Cryopreservation has been proven significance as a technique for promising the long-term conservation of plant germplasms. This study aimed to establish a cryopreservation protocol for calli of Schisandra chinensis (Turcz.) Baill, and to explore the effects of different process parameters on callus viability. Effects of desiccation duration, cryoprotectants and cryopreservation methods, thawing temperature, and post-culture conditions on the viability of cryopreserved calli were assessed. Among different cryoprotectants and freezing procedures, the highest survival was recorded when the water content of callus after 30 min desiccation was 57.3%, were loaded into a cryoprotectant containing 10% ethylene glycol, 8% glucose, and 10% DMSO, and frozen slowly (-1°C/min). Rapid thawing at 40°C for 2 min demonstrated the best recovery of cryopreserved S. chinensis calli. Post-culturing in darkness for one week before transfer to light conditions (under 16 h photoperiod at 36 µmol·m-2·s-1) was beneficial to callus regeneration. Plants regenerated through somatic embryogenesis from cryopreserved calli remained ploidy stable after cryopreservation. The callus cryopreservation procedure established in this study is a promising tool for the conservation of S. chinensis resources.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122776398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic variability affecting Exserohilum turcicum resistance in popcorn lines grown under high and low phosphorus conditions. 高、低磷条件下玉米品系抗灰枯病的遗传变异

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049399

A. T. Amaral, R. Ribeiro, P. H. Santos, T. P. S. Poltronieri, J. M. S. Vivas, I. F. Gerhardt, B. M. Carvalho, C. S. Freitas, S. B. Miranda

{"title":"Genetic variability affecting Exserohilum turcicum resistance in popcorn lines grown under high and low phosphorus conditions.","authors":"A. T. Amaral, R. Ribeiro, P. H. Santos, T. P. S. Poltronieri, J. M. S. Vivas, I. F. Gerhardt, B. M. Carvalho, C. S. Freitas, S. B. Miranda","doi":"10.4238/gmr15049399","DOIUrl":"https://doi.org/10.4238/gmr15049399","url":null,"abstract":"Northern leaf blight (NLB), caused by Exserohilum turcicum, is one of the main foliar diseases that affect popcorn culture. Farmers use many control measures to minimize damage caused by this disease, among which, the use of cultivars with genetic resistance is the most effective and economical. The aim of this study was to investigate genetic variability influencing resistance to NLB in 25 popcorn maize lines grown under high and low phosphorus conditions in relation to foliar fungal disease caused by E. turcicum. We evaluated the disease incidence and severity, by analysis of variance and cluster test (Scott-Knott). There was sufficient genetic variability between strains for resistance traits. Genotypic variance was higher than environmental variance, and had more discriminatory power. We conclude that new progenies could be selected for the establishment of future populations. P-7, P-9, L-59, L-71, and L-76 progenies possess promising characteristics that simultaneously reduce the severity and the incidence of NLB in popcorn plants.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"15 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129810351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Targeting of miR-432 to myozenin1 to regulate myoblast proliferation and differentiation. miR-432靶向myozenin1调控成肌细胞增殖和分化。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049313

R. Ren, H. Liu, Shuhong Zhao, J. H. Cao

{"title":"Targeting of miR-432 to myozenin1 to regulate myoblast proliferation and differentiation.","authors":"R. Ren, H. Liu, Shuhong Zhao, J. H. Cao","doi":"10.4238/gmr15049313","DOIUrl":"https://doi.org/10.4238/gmr15049313","url":null,"abstract":"MicroRNAs (miRNAs) play important roles in the development and biochemical functions of skeletal muscles. However, targeting of miRNAs to structural genes involved in Z-discs have not been investigated. Here, we describe a highly expressed miRNA, miR-432, in pig embryonic skeletal muscle, which appeared to target myozenin1 (MYOZ1), a protein involved in the muscular sarcomere microstructure. Our results showed that miR-432 is involved in muscle development in the developing pig. In addition, it promoted differentiation of the C2C12 myoblast cell line into myotubes. We also demonstrated that inhibition of miR-432 reduced proliferation of C2C12 cells, suggesting that miR-432 is involved in regulation of myoblast proliferation. Moreover, molecular markers of muscle differentiation and fiber type (Myh7/ slow and Myh4/ fast IIB) showed that miR-432 reduced the differentiation rate of C2C12 cells. These results provide insights into the potential functions of miR-432 as well as its proposed target, MYOZ1, during muscle development. This may lead to applications for further improvements in porcine muscle growth, and may enhance our understanding of complex inherited human muscle disorders.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127234700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Blood folic acid, vitamin B12, and homocysteine levels in pregnant women with fetal growth restriction. 胎儿生长受限孕妇血叶酸、维生素B12和同型半胱氨酸水平。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15048890

H. Jiang, L. Cao, H. Y. Chen

{"title":"Blood folic acid, vitamin B12, and homocysteine levels in pregnant women with fetal growth restriction.","authors":"H. Jiang, L. Cao, H. Y. Chen","doi":"10.4238/gmr15048890","DOIUrl":"https://doi.org/10.4238/gmr15048890","url":null,"abstract":"Deficiencies in nutrients such as folic acid and vitamin B12 may play a role in fetal growth restriction (FGR). However, whether folic acid, vitamin B12, or homocysteine is associated with FGR in Chinese populations remains unclear. This study investigated the relationship between these nutrient deficiencies and FGR in pregnant Chinese women. We selected 116 mother and infant pairs, and categorized the neonates into the FGR, appropriate for gestational age, and large for gestational age groups. Birth weight, body length, head circumference, body mass index (BMI), and Rohrer's body index of the newborns were measured. Serum folic acid, vitamin B12, and homocysteine levels were measured in mothers during the first three days of their hospital stay. Results showed that the FGR group exhibited reduced folic acid and vitamin B12 levels and elevated homocysteine levels than those in the other two groups. Folic acid and vitamin B12 levels were positively correlated with birth weight, head circumference, and BMI, whereas homocysteine level was negatively correlated with these variables. The FGR ratio in the folic acid and vitamin B12 deficiency group was higher than that in the sufficiency group (χ2 = 4.717 and 4.437, P = 0.029 and 0.035, respectively). In addition, elevated homocysteine was associated with FGR (χ2 = 5.366, P = 0.021). In conclusion, we found that folic acid and vitamin B12 deficiency was associated with elevated homocysteine levels, which may increase susceptibility to FGR.","PeriodicalId":189314,"journal":{"name":"Genetics and molecular research : GMR","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132610770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Proposal of a super trait for the optimum selection of popcorn progenies based on path analysis. 基于通径分析的爆米花子代优选超级性状的提出。

Genetics and molecular research : GMR Pub Date : 2016-12-19 DOI: 10.4238/gmr15049309

A. Júnior, A. Santos, I. F. Gerhardt, R. N. F. Kurosawa, N. F. Moreira, M. G. Pereira, G. Gravina, F. L. Silva

引用次数: 19