五味子的低温保存球茎愈伤组织和随后的植株再生。

D. Sun, Y-F Yu, H. Qin, P. Xu, Y. Zhao, Y.X. Liu, Z. Wang, S. Fan, Y.M. Yang, J. Ai
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引用次数: 1

摘要

超低温保存作为一种有前景的植物种质资源的长期保存技术已被证明具有重要意义。本研究旨在建立五味子愈伤组织的低温保存方法。并探讨不同工艺参数对愈伤组织活力的影响。评估了干燥时间、冷冻保护剂和冷冻保存方法、解冻温度和培养后条件对冷冻愈伤组织活力的影响。在不同的冷冻保护剂和冷冻程序中,当愈伤组织干燥30 min后含水量为57.3%时,将其装入含有10%乙二醇、8%葡萄糖和10% DMSO的冷冻保护剂中,缓慢冷冻(-1°C/min),存活率最高。在40℃快速解冻2min后,冬青愈伤组织恢复最好。培养1周后转入光照条件下(光照条件为36µmol·m-2·s-1,光周期为16 h)有利于愈伤组织再生。愈伤组织体细胞胚再生植株经低温保存后,其倍性保持稳定。本研究建立的愈伤组织低温保存方法是一种很有前途的保护紫荆资源的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryopreservation of Schisandra chinensis (Turcz.) Baill callus and subsequent plant regeneration.
Cryopreservation has been proven significance as a technique for promising the long-term conservation of plant germplasms. This study aimed to establish a cryopreservation protocol for calli of Schisandra chinensis (Turcz.) Baill, and to explore the effects of different process parameters on callus viability. Effects of desiccation duration, cryoprotectants and cryopreservation methods, thawing temperature, and post-culture conditions on the viability of cryopreserved calli were assessed. Among different cryoprotectants and freezing procedures, the highest survival was recorded when the water content of callus after 30 min desiccation was 57.3%, were loaded into a cryoprotectant containing 10% ethylene glycol, 8% glucose, and 10% DMSO, and frozen slowly (-1°C/min). Rapid thawing at 40°C for 2 min demonstrated the best recovery of cryopreserved S. chinensis calli. Post-culturing in darkness for one week before transfer to light conditions (under 16 h photoperiod at 36 µmol·m-2·s-1) was beneficial to callus regeneration. Plants regenerated through somatic embryogenesis from cryopreserved calli remained ploidy stable after cryopreservation. The callus cryopreservation procedure established in this study is a promising tool for the conservation of S. chinensis resources.
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