IF 1.2 4区生物学

Nematology Pub Date : 2024-08-08 DOI: 10.1163/15685411-bja10345

J. Abolafia, A. N. Ruiz-Cuenca, Thi Anh Duong Nguyen

引用次数: 0

Unveiling novel and known Cryptaphelenchus species from China and USA 揭开中国和美国已知隐翅虫新物种的面纱

IF 1.2 4区生物学

Nematology Pub Date : 2024-06-13 DOI: 10.1163/15685411-bja10335

Jianfeng Gu, P. Castillo, Xinxin Ma, M. Munawar

{"title":"Unveiling novel and known Cryptaphelenchus species from China and USA","authors":"Jianfeng Gu, P. Castillo, Xinxin Ma, M. Munawar","doi":"10.1163/15685411-bja10335","DOIUrl":"https://doi.org/10.1163/15685411-bja10335","url":null,"abstract":"\u0000Cryptaphelenchus species are wood- or bark-inhabiting nematodes, generally mycetophagous and reported to have endophoretic associations with insects. In the present study, we describe two new and one known Cryptaphelenchus species detected in imported and domestic wood samples. Cryptaphelenchus americanum n. sp. and C. minutus were detected in the log samples of Pinus taeda imported from the USA, whereas C. orientalis n. sp. was isolated from the bark of dying Pinus sylvestris trees in Inner Mongolia, China. Both new species displayed characteristic features, including a relatively short body length, four lateral lines, a short post-vulval uterine sac in females, and a distinct cloacal apophysis in male tails. Newly recovered and known species were characterised molecularly, and phylogenetic trees were constructed to study their relationship with related Cryptaphelenchus species. Notably, the genetic divergence observed among Cryptaphelenchus species was found to be more significant compared to morphometrical differences, highlighting the importance of molecular data in taxonomy. The identification of new and known species expands our understanding of the genus and suggests that Cryptaphelenchus species may be under studied, underscoring the necessity for continued exploration.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skrjabinomermis pirnaimi sp. n. (Nematoda: Mermithidae) from Iran: A morphological and molecular phylogenetic study Skrjabinomermis pirnaimi sp：形态学和分子系统学研究

IF 1.2 4区生物学

Nematology Pub Date : 2024-06-13 DOI: 10.1163/15685411-bja10336

F. Abootalebi, G. Niknam, N. Vazifeh, N. Camino, Saideh Shaaf Gorashi

{"title":"Skrjabinomermis pirnaimi sp. n. (Nematoda: Mermithidae) from Iran: A morphological and molecular phylogenetic study","authors":"F. Abootalebi, G. Niknam, N. Vazifeh, N. Camino, Saideh Shaaf Gorashi","doi":"10.1163/15685411-bja10336","DOIUrl":"https://doi.org/10.1163/15685411-bja10336","url":null,"abstract":"\u0000A new species of the genus Skrjabinomermis, collected from two locations in north and northwest of Iran, is described and illustrated, including morphological data and D2-D3 rRNA LSU sequences. Skrjabinomermis pirnaimi sp. n. is characterised by its six cephalic papillae, two hypodermal pegs in the area of the cephalic papillae, adult’s cuticle with very fine cross fibres, head rounded, mouth central, amphids cup-shaped, six hypodermal cords, vulva a transverse slit, located post-equatorially, cuticular protruding and vulval lips well developed, vagina pear- or barrel-shaped, with slightly bent lumen, tail similar in both sexes, bluntly rounded, spicules paired, medium sized, slightly curved, fused posteriorly for about 68-73% of their length, with pointed tip, male genital papillae arranged in three rows: the median ventral row having two pre- and two post-anal papillae, and the lateroventral rows with five papillae each. Molecular analyses using the D2-D3 expansion regions of the 28S rRNA gene sequences placed the genus Skrjabinomermis as a sister genus to Romanomermis with 93% Bayesian posterior probability based upon currently available data.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delving into the diversity of genus Ironus Bastian, 1865 with special reference to Ironus dentifurcatus Argo & Heyns, 1972 (Ironidae: Enoplida), collected from coal mines 深入探讨 Ironus Bastian, 1865 属的多样性，特别关注从煤矿采集的 Ironus dentifurcatus Argo & Heyns, 1972 (Ironidae: Enoplida)

IF 1.2 4区生物学

Nematology Pub Date : 2024-06-05 DOI: 10.1163/15685411-bja10331

Mohammad Asif, M. Mahboob, P. F. Rehman, Q. Tahseen

引用次数: 0

Unveiling two novel Ditylenchus (Nematoda: Anguinidae) species inhabiting the growing medium of Rhododendron simsii imported from Japan 揭示栖息于日本进口杜鹃花生长介质中的两个新的根瘤蚤（线虫纲：鳗鲡科）物种

IF 1.2 4区生物学

Nematology Pub Date : 2024-06-04 DOI: 10.1163/15685411-bja10332

J. Gu, Xinxin Ma, P. Castillo, M. Munawar

{"title":"Unveiling two novel Ditylenchus (Nematoda: Anguinidae) species inhabiting the growing medium of Rhododendron simsii imported from Japan","authors":"J. Gu, Xinxin Ma, P. Castillo, M. Munawar","doi":"10.1163/15685411-bja10332","DOIUrl":"https://doi.org/10.1163/15685411-bja10332","url":null,"abstract":"\u0000The present study describes two new Ditylenchus species, isolated from the growing medium of Rhododendron simsii imported from Japan, at the Ningbo plant quarantine laboratory, Ningbo, P.R. China. Ditylenchus parvicauda n. sp. is an amphimictic species. Females have six incisures in the lateral fields, a medium length slim body, a short delicate stylet with asymmetrical stylet knobs, the secretory-excretory (S-E) pore located posterior to the isthmus and in the anterior region of the basal pharyngeal bulb, a posteriorly located vulva (V = 80.9-83.0), post-uterine sac (PUS) tube-like, and the tail cylindrical, tapering gradually towards finely rounded terminus. Males are similar to females in general morphology and have 10.1-14.9 μm long spicules. The second new species, D. gracicauda n. sp. is characterised by having a long and slender body, the presence of a vulval flap, four lines in the lateral field, a narrow and low lip region not offset, the S-E pore located posterior to the isthmus but not in the anterior region of the basal pharyngeal bulb, a posteriorly located vulva (V = 71.1-76.2), a short post-vulval uterine sac short (4.5-9.2 μm), and the tail slender, tapering gradually towards finely rounded to wedge-shaped terminus. The two new species were also characterised molecularly using 18S, ITS and the D2-D3 expansion segments of 28S rRNA genes. Our phylogenetic analyses showed their independent place among available Ditylenchus species. Several Ditylenchus species are regulated pests, and the introduction of these species into a new environment may result in diseases among native plants or vegetation in the area. Therefore, careful attention must be directed towards newly discovered species, particularly those under quarantine observation.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141387838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Longidorus sanlitanensis n. sp. (Dorylaimida: Longidoridae) from the rhizosphere of Melilotoides ruthenica and Chamaerhodos erecta in China 描述中国三棱苣苔和直立苣苔根瘤中的三棱苣苔(Dorylaimida: Longidoridae)

IF 1.2 4区生物学

Nematology Pub Date : 2024-06-04 DOI: 10.1163/15685411-bja10333

Ruihang Cai, J. Gu, W. Ye, Xinxin Ma, Yinzhan Liu

{"title":"Description of Longidorus sanlitanensis n. sp. (Dorylaimida: Longidoridae) from the rhizosphere of Melilotoides ruthenica and Chamaerhodos erecta in China","authors":"Ruihang Cai, J. Gu, W. Ye, Xinxin Ma, Yinzhan Liu","doi":"10.1163/15685411-bja10333","DOIUrl":"https://doi.org/10.1163/15685411-bja10333","url":null,"abstract":"\u0000Longidorus sanlitanensis n. sp. was recovered from the rhizosphere of Melilotoides ruthenica and Chamaerhodos erecta in the Inner Mongolia Autonomous Region, P.R. China, and was described and illustrated using morphological and molecular data. The new species is characterised by medium body length of females (4.0-4.8 mm), lip region anteriorly rounded and separated from the rest of the body by a depression, pocket-shaped amphidial pouches, the guiding ring located at 58.4-66.0 μm from the anterior end, odontostyle and odontophore 107.2-114.6 and 71.6-77.8 μm long, respectively, the pharyngeal bulb 68.7-79.7 μm long, a short conical tail with a broadly rounded terminus, four juvenile developmental stages (J1 was not detected) and males absent. By similarities in the distance of the guiding ring from the anterior body end, body and odontostyle length and lip region shape, the new species was compared with nine similar species, namely: L. litchi, L. diadecturus, L. fursti, L. himalayensis, L. jonesi, L. wiesae, L. macromucronatus, L. fangi and L. zhengi. The matrix code of the new species is: A4-B2-C5-D4-E1-F2-G2-H12-I2-J1-K?. The phylogenetic relationships of the new species with other relevant species were reconstructed using near-full-length 18S, ITS1 and 28S D2-D3 regions of rRNA gene and the resulted topologies were discussed. In 18S phylogeny, L. sanlitanensis n. sp. formed a clade with L. litchi inside a major clade, including species with a posteriorly located guiding ring, and in 28S phylogeny it showed close relationships with species having a posteriorly located guiding ring. This is the 22nd Longidorus species recorded from China.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141386830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation and diagnostics of the red ring nematode, Bursaphelenchus cocophilus, from Mexico 墨西哥红环线虫 Bursaphelenchus cocophilus 的分子特征和诊断方法

IF 1.2 4区生物学

Nematology Pub Date : 2024-05-13 DOI: 10.1163/15685411-bja10329

S. Subbotin, Ignacio Cid del Prado-Vera

{"title":"Molecular characterisation and diagnostics of the red ring nematode, Bursaphelenchus cocophilus, from Mexico","authors":"S. Subbotin, Ignacio Cid del Prado-Vera","doi":"10.1163/15685411-bja10329","DOIUrl":"https://doi.org/10.1163/15685411-bja10329","url":null,"abstract":"\u0000The red ring nematode, Bursaphelenchus cocophilus, vectored by the South American palm weevil, Rhynchophorus palmarum, is the causal agent of red ring disease in coconut and other palms in countries of Central and South America. The populations of B. cocophilus collected in the states of Guerrero and Tabasco, Mexico, were molecularly characterised using the D2-D3 expansion segments of 28S rRNA, ITS rRNA and COI gene sequences. Mexican B. cocophilus populations were molecularly different from other Central and South American populations. Comparative analysis of available rRNA sequences obtained from several countries showed that B. cocophilus consists of molecularly different populations and its species structure is likely congruent with that of the beetle vector. Conventional PCR, real-time PCR and recombinase polymerase amplification (RPA) with lateral flow dipstick (LF) assays have been developed for the identification of the red ring nematode in this study. The specificity of the ITS rRNA primers in the assays were examined using B. cocophilus and other 15 species of family Aphelenchoididae. Detection sensitivity levels, determined by using a dilution series of B. cocophilus extracts, was 0.13 nematode per reaction tube for real-time PCR and 0.25 nematode per reaction tube for conventional PCR and LF-RPA assays. The application of the LF-RPA assay has great potential for diagnosing infestation of this species with a minimal laboratory infrastructure.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140985966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bursaphelenchus glaucae n. sp. isolated from larval faeces of a longhorn beetle, Aeolesthes chrysothrix chrysothrix (Bates), in its pupal chamber constructed in the dead wood of Quercus glauca Thunb. from Japan 从日本长角甲虫 Aeolesthes chrysothrix chrysothrix (Bates) 在柞树枯木中建造的蛹室中分离出的 Bursaphelenchus glaucae n. sp.

IF 1.2 4区生物学

Nematology Pub Date : 2024-05-10 DOI: 10.1163/15685411-bja10327

N. Kanzaki, Yuta Fujimori

{"title":"Bursaphelenchus glaucae n. sp. isolated from larval faeces of a longhorn beetle, Aeolesthes chrysothrix chrysothrix (Bates), in its pupal chamber constructed in the dead wood of Quercus glauca Thunb. from Japan","authors":"N. Kanzaki, Yuta Fujimori","doi":"10.1163/15685411-bja10327","DOIUrl":"https://doi.org/10.1163/15685411-bja10327","url":null,"abstract":"\u0000A Bursaphelenchus species belonging to the hofmanni group (corneolus subgroup) was isolated from a small piece of larval faeces of the longhorn beetle Aeolesthes chrysothrix chrysothrix in its pupal chamber. The chamber was constructed in a dead wood section of Quercus glauca obtained from a live tree. The material was obtained during a management procedure of a garden tree to remove the dead part from live tree. The species is typologically characterised by its three-lined lateral field, the position of its secretory-excretory pore overlapping with the metacorpus anterior half, a very small spicule with distinctive broad and rounded condyles, a flattened trapezoid-shaped rostrum and small flattened cucullus, the presence of seven (P1, P2 and P3 papilliform and P5 gland) male genital papillae, a female vulva with a side flap and female tail forming an elongate conoid with a strongly ventrally curved posterior half and narrowly rounded or digitate tip. The species is typologically close to B. corneolus, sharing a small cucullus and female tail shape, but can be distinguished from the other species by the position of the secretory-excretory pore and the spicule rostrum shape. Phylogenetically, the new species forms a well-supported clade with B. corneolus, but can be distinguished by its clearly separable phylogenetic status inferred from SSU and D2-D3 LSU sequences and the difference in the ITS sequence. The new species is described as B. glaucae n. sp. Although the nematode was isolated from the larval faeces of A. c. chrysothrix, it has not been directly isolated from the insect body to date, thus, the carrier insect of the nematode has not been determined.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140990952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pristionchus seladoniae n. sp. (Diplogastridae) isolated from a eusocial soil-dwelling bee, Halictus (Seladonia) aerarius, in Nagano, Japan Pristionchus seladoniae n. sp.（Diplogastridae），从日本长野的一种社会性土栖蜜蜂 Halictus (Seladonia) aerarius 身上分离出来

IF 1.2 4区生物学

Nematology Pub Date : 2024-05-10 DOI: 10.1163/15685411-bja10326

N. Kanzaki, Yuta Fujimori, Taisuke Ekino, Yousuke Degawa

{"title":"Pristionchus seladoniae n. sp. (Diplogastridae) isolated from a eusocial soil-dwelling bee, Halictus (Seladonia) aerarius, in Nagano, Japan","authors":"N. Kanzaki, Yuta Fujimori, Taisuke Ekino, Yousuke Degawa","doi":"10.1163/15685411-bja10326","DOIUrl":"https://doi.org/10.1163/15685411-bja10326","url":null,"abstract":"\u0000A new species of Pristionchus was isolated from a soil-dwelling bee, Halictus (Seladonia) aerarius, collected from Sugadaira, Nagano, Japan. The nematode was successfully cultured on nematode growth medium agar previously inoculated with the Escherichia coli strain OP50; however, it did not propagate well. Typologically, the new species is similar to other Pristionchus belonging to the maupasi, pacificus and lheritieri groups in the stomatal structure of stenostomatous form. However, the species is characterised by the relatively short, conical tail of the female and the arrangement of male genital papillae in which the second- and third-paired papillae are almost at the same level and one of these two are directed laterally. Phylogenetically, the nematode belongs to the maupasi group, the ‘American clade’ within the genus, and is separable from the other species in the group. Biologically, the species has several interesting characters: it is seemingly native to Japan, regardless of its phylogenetic group; it is in tight and specific phoretic association with a eusocial bee; it lacks a eurystomatous form (or this form occurs rarely) under cultured conditions; and it shows characteristic clumping behaviour on culture media. The new species is taxonomically described herein as Pristionchus seladoniae n. sp.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140992720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cotylenchus cleo gen. n., sp. n., a new plant-parasitic nematode (Tylenchida: Anguinidae) parasitising on leaves of western sword fern plants from rainforests in Washington State, USA Cotylenchus cleo gen.

IF 1.2 4区生物学

Nematology Pub Date : 2024-05-01 DOI: 10.1163/15685411-bja10325

S. Álvarez‐Ortega, S. Subbotin

{"title":"Cotylenchus cleo gen. n., sp. n., a new plant-parasitic nematode (Tylenchida: Anguinidae) parasitising on leaves of western sword fern plants from rainforests in Washington State, USA","authors":"S. Álvarez‐Ortega, S. Subbotin","doi":"10.1163/15685411-bja10325","DOIUrl":"https://doi.org/10.1163/15685411-bja10325","url":null,"abstract":"\u0000A new genus and species of anguinid nematode, Cotylenchus cleo gen. n., sp. n., was recovered from leaves of the western sword fern, Polystichum munitum, growing in rainforests in several locations of Olympic National Park, Washington State, USA. This new anguinid nematode induced distinct necrotic and chlorotic symptoms on fern leaves characterised by yellowing, light to brown areas between veins. The new species is characterised by a long and slender body, six incisures in the lateral field, robust stylet (12.0-13.5 μm) with large and rounded knobs, pyriform to elongate and abutting basal pharyngeal bulb, and long conical tail with pointed terminus; females having posteriorly located vulva (V = 74-80%) and well-developed post-vulval uterine sac (26-59 μm) and males having spicules 20-23 μm long. Phylogenetic analysis of the partial 18S rRNA and the D2-D3 expansion segments of the 28S rRNA genes showed that this anguinid nematode formed a separate evolutionary lineage different from all other Anguinidae taxa. The new species was also characterised by sequencing the ITS rRNA and COI genes.","PeriodicalId":18928,"journal":{"name":"Nematology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141038444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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