Molecular Membrane Biology最新文献_第6页

Lipase activity in lipidomics - a hidden problem? 脂质组学中的脂肪酶活性——一个隐藏的问题?

Molecular Membrane Biology Pub Date : 2013-11-01 Epub Date: 2013-09-09 DOI: 10.3109/09687688.2013.835125

Samuel Furse, J Antoinette Killian

引用次数: 4

How do transporters couple solute movements? 转运体是如何结合溶质运动的?

Molecular Membrane Biology Pub Date : 2013-11-01 DOI: 10.3109/09687688.2013.842658

Gary Rudnick

引用次数: 16

Efficiency of the monofunctionalized C60 fullerenes as membrane targeting agents studied by all-atom molecular dynamics simulations. 用全原子分子动力学模拟研究了单官能化C60富勒烯作为膜靶向剂的效率。

Molecular Membrane Biology Pub Date : 2013-08-01 DOI: 10.3109/09687688.2013.828856

Semen O Yesylevskyy, Sebastian Kraszewski, Fabien Picaud, Christophe Ramseyer

引用次数: 5

Expression, characterization and ligand specificity of lipocalin-1 interacting membrane receptor (LIMR). 脂钙素-1相互作用膜受体(LIMR)的表达、表征及配体特异性。

Molecular Membrane Biology Pub Date : 2013-08-01 DOI: 10.3109/09687688.2013.823018

Renske W Hesselink, John B C Findlay

{"title":"Expression, characterization and ligand specificity of lipocalin-1 interacting membrane receptor (LIMR).","authors":"Renske W Hesselink, John B C Findlay","doi":"10.3109/09687688.2013.823018","DOIUrl":"https://doi.org/10.3109/09687688.2013.823018","url":null,"abstract":"Human lipocalin-1 interacting membrane receptor (LIMR) was the first lipocalin receptor to be identified, as a specific receptor for lipocalin-1 (Lcn1). Subsequently LIMR has been reported to interact with other ligands as well, notably with the bovine lipocalin β-lactoglobulin (BLG) and with the unrelated secretoglobin uteroglobin (UG). To study the ligand-binding behaviour of this prototypic lipocalin receptor in more detail, a system was developed for the recombinant expression of LIMR in Drosophila Schneider 2 (S2) cells, and for the subsequent solubilization and purification of the protein. The receptor forms dimers or larger oligomers when solubilized in n-dodecyl β-D-maltoside (DDM). The full-length, functional receptor was captured onto a surface plasmon resonance (SPR) chip via an α-V5 antibody, and the binding of various potential ligands was followed in time. In this way, LIMR was shown to be highly specific for Lcn1, binding the lipocalin with low micromolar to high nanomolar affinity. No interactions with any of the other putative ligands could be detected, raising doubts about the physiological relevance of the reported binding of BLG and UG to the receptor.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 5-6","pages":"327-37"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.823018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31673487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Lysophosphatidylcholine-induced cytotoxicity in osteoblast-like MG-63 cells: involvement of transient receptor potential vanilloid 2 (TRPV2) channels. 溶血磷脂酰胆碱诱导成骨细胞样MG-63细胞的细胞毒性:瞬时受体电位香兰素2 (TRPV2)通道的参与

Molecular Membrane Biology Pub Date : 2013-08-01 DOI: 10.3109/09687688.2013.828855

Abdallah Fallah, Rachel Pierre, Elie Abed, Robert Moreau

{"title":"Lysophosphatidylcholine-induced cytotoxicity in osteoblast-like MG-63 cells: involvement of transient receptor potential vanilloid 2 (TRPV2) channels.","authors":"Abdallah Fallah, Rachel Pierre, Elie Abed, Robert Moreau","doi":"10.3109/09687688.2013.828855","DOIUrl":"https://doi.org/10.3109/09687688.2013.828855","url":null,"abstract":"Epidemiological studies indicate that patients suffering from atherosclerosis are predisposed to develop osteoporosis. Accordingly, atherogenic determinants such as oxidized low density lipoprotein (OxLDL) particles have been shown to alter bone cell functions. In this work, we investigated the cytotoxicity of lysophosphatidylcholine (lysoPC), a major phospholipid component generated upon LDL oxidation, on bone-forming MG-63 osteoblast-like cells. Cell viability was reduced by lysoPC in a concentration-dependent manner with a LC50 of 18.7±0.7 μM. LysoPC-induced cell death was attributed to induction of both apoptosis and necrosis. Since impairment of intracellular calcium homeostasis is often involved in mechanism of cell death, we determined the involvement of calcium in lysoPC-induced cytotoxicity. LysoPC promoted a rapid and transient increase in intracellular calcium attributed to mobilization from calcium stores, followed by a sustained influx. Intracellular calcium mobilization was associated to phospholipase C (PLC)-dependent mobilization of calcium from the endoplasmic reticulum since inhibition of PLC or calcium depletion of reticulum endoplasmic with thapsigargin prevented the calcium mobilization. The calcium influx induced by lysoPC was abolished by inhibition of transient receptor potential vanilloid (TRPV) channels with ruthenium red whereas gadolinium, which inhibits canonical TRP (TRPC) channels, was without effect. Accordingly, expression of TRPV2 and TRPV4 were shown in MG-63 cells. The addition of TRPV2 inhibitor Tranilast in the incubation medium prevent the calcium influx triggered by lysoPC and reduced lysoPC-induced cytotoxicity whereas TRPV4 inhibitor RN 1734 was without effect, which confirms the involvement of TRPV2 activation in lysoPC-induced cell death.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 5-6","pages":"315-26"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.828855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31673488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Hydrophobic segment of dengue virus C protein. Interaction with model membranes. 登革病毒C蛋白疏水片段。与模型膜的相互作用。

Molecular Membrane Biology Pub Date : 2013-07-01 Epub Date: 2013-06-07 DOI: 10.3109/09687688.2013.805835

Henrique Nemésio, M Francisca Palomares-Jerez, José Villalaín

{"title":"Hydrophobic segment of dengue virus C protein. Interaction with model membranes.","authors":"Henrique Nemésio, M Francisca Palomares-Jerez, José Villalaín","doi":"10.3109/09687688.2013.805835","DOIUrl":"https://doi.org/10.3109/09687688.2013.805835","url":null,"abstract":"Dengue virus (DENV) C protein is essential for viral assembly. DENV C protein associates with intracellular membranes through a conserved hydrophobic domain and accumulates around endoplasmic reticulum-derived lipid droplets which could provide a platform for capsid formation during assembly. In a previous work we described a region in DENV C protein which induced a nearly complete membrane rupture of several membrane model systems, which was coincident with the theoretically predicted highly hydrophobic region of the protein. In this work we have carried out a study of the binding to and interaction with model biomembranes of a peptide corresponding to this DENV C region, DENV2C6. We show that DENV2C6 partitions into phospholipid membranes, is capable of rupturing membranes even at very low peptide-to-lipid ratios and its membrane-activity is modulated by lipid composition. These results identify an important region in the DENV C protein which might be directly implicated in the DENV life cycle through the modulation of membrane structure.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 4","pages":"273-87"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.805835","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31488317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Rab1a and Rab5a preferentially bind to binary lipid compositions with higher stored curvature elastic energy. Rab1a和Rab5a优先结合具有较高曲率弹性能的二元脂质组合物。

Molecular Membrane Biology Pub Date : 2013-07-01 DOI: 10.3109/09687688.2013.818725

Marie L Kirsten, Rudi A Baron, Miguel C Seabra, Oscar Ces

{"title":"Rab1a and Rab5a preferentially bind to binary lipid compositions with higher stored curvature elastic energy.","authors":"Marie L Kirsten, Rudi A Baron, Miguel C Seabra, Oscar Ces","doi":"10.3109/09687688.2013.818725","DOIUrl":"https://doi.org/10.3109/09687688.2013.818725","url":null,"abstract":"Abstract Rab proteins are a large family of GTP-binding proteins that regulate cellular membrane traffic and organelle identity. Rab proteins cycle between association with membranes and binding to RabGDI. Bound on membranes, each Rab has a very specific cellular location and it is this remarkable degree of specificity with which Rab GTPases recognize distinct subsets of intracellular membranes that forms the basis of their ability to act as key cellular regulators, determining the recruitment of downstream effectors to the correct membrane at the correct time. The molecular mechanisms controlling Rab localization remain poorly understood. Here, we present a fluorescence-based assay to investigate Rab GTPase membrane extraction and delivery by RabGDI. Using EGFP-Rab fusion proteins the amount of Rab:GDI complex obtained by GDI extraction of Rab proteins from HEK293 membranes could be determined, enabling control of complex concentration. Subsequent partitioning of the Rab GTPases into vesicles made up of artificial binary lipid mixtures showed for the first time, that the composition of the target membrane plays a key role in the localization of Rab proteins by sensing the stored curvature elastic energy in the membrane.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 4","pages":"303-14"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.818725","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31546885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Close allies in membrane protein research: cell-free synthesis and nanotechnology. 膜蛋白研究的紧密盟友:无细胞合成和纳米技术。

Molecular Membrane Biology Pub Date : 2013-05-01 Epub Date: 2013-01-24 DOI: 10.3109/09687688.2012.762125

Nadim Shadiac, Yagnesh Nagarajan, Shane Waters, Maria Hrmova

{"title":"Close allies in membrane protein research: cell-free synthesis and nanotechnology.","authors":"Nadim Shadiac, Yagnesh Nagarajan, Shane Waters, Maria Hrmova","doi":"10.3109/09687688.2012.762125","DOIUrl":"https://doi.org/10.3109/09687688.2012.762125","url":null,"abstract":"Membrane proteins control fundamental processes that are inherent to nearly all forms of life such as transport of molecules, catalysis, signaling, vesicle fusion, sensing of chemical and physical stimuli from the environment, and cell-cell interactions. Membrane proteins are harbored within a non-equilibrium fluid-like environment of biological membranes that separate cellular and non-cellular environments, as well as in compartmentalized cellular organelles. One of the classes of membrane proteins that will be specifically treated in this article are transport proteins of plant origin, that facilitate material and energy transfer at the membrane boundaries. These proteins import essential nutrients, export cellular metabolites, maintain ionic and osmotic equilibriums and mediate signal transduction. The aim of this article is to report on the progress of membrane protein functional and structural relationships, with a focus on producing stable and functional proteins suitable for structural and biophysical studies. We interlink membrane protein production primarily through wheat-germ cell-free protein synthesis (WG-CFPS) with the growing repertoire of membrane mimicking environments in the form of lipids, surfactants, amphipathic surfactant polymers, liposomes and nanodiscs that keep membrane proteins soluble. It is hoped that the advancements in these fields could increase the number of elucidated structures, in particular those of plant membrane proteins, and contribute to bridging of the gap between structures of soluble and membrane proteins, the latter being comparatively low.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 3","pages":"229-45"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2012.762125","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31182157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

The identification of novel, high affinity AQP9 inhibitors in an intracellular binding site. 在细胞内结合位点鉴定新的高亲和力AQP9抑制剂。

Molecular Membrane Biology Pub Date : 2013-05-01 Epub Date: 2013-03-01 DOI: 10.3109/09687688.2013.773095

Sören J Wacker, Camilo Aponte-Santamaría, Per Kjellbom, Søren Nielsen, Bert L de Groot, Michael Rützler

{"title":"The identification of novel, high affinity AQP9 inhibitors in an intracellular binding site.","authors":"Sören J Wacker, Camilo Aponte-Santamaría, Per Kjellbom, Søren Nielsen, Bert L de Groot, Michael Rützler","doi":"10.3109/09687688.2013.773095","DOIUrl":"https://doi.org/10.3109/09687688.2013.773095","url":null,"abstract":"Background: The involvement of aquaporin (AQP) water and small solute channels in the etiology of several diseases, including cancer, neuromyelitis optica and body fluid imbalance disorders, has been suggested previously. Furthermore, results obtained in a mouse model suggested that AQP9 function contributes to hyperglycemia in type-2 diabetes. In addition, the physiological role of several AQP family members remains poorly understood. Small molecule inhibitors of AQPs are therefore desirable to further study AQP physiological and pathophysiological functions.Methods: The binding of recently established AQP9 inhibitors to a homology model of AQP9 was investigated by molecular dynamics simulations and molecular docking. Putative inhibitor binding sites identified with this procedure were modified by site-directed mutagenesis. Active compounds were measured in a mammalian cell water permeability assay of mutated AQP9 isoforms and tested for changes in inhibitory effects.Controls: Three independent cell lines were established for each mutated AQP9 isoform and functionality of mutant isoforms was established.Principal findings: We have identified putative binding sites of recently established AQP9 inhibitors. This information facilitated successful identification of novel AQP9 inhibitors with low micromolar IC50 values in a cell based assay by in silico screening of a compound library targeting specifically this binding site.Significance: We have established a successful strategy for AQP small molecule inhibitor identification. AQP inhibitors may be relevant as experimental tools, to enhance our understanding of AQP function, and in the treatment of various diseases.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 3","pages":"246-60"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.773095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31271243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Permeabilization of enterocytes induced by absorption of dietary fat. 饮食脂肪吸收诱导肠细胞通透性。

Molecular Membrane Biology Pub Date : 2013-05-01 Epub Date: 2013-03-26 DOI: 10.3109/09687688.2013.780642

Erik Michael Danielsen, Gert H Hansen, Karina Rasmussen, Lise-Lotte Niels-Christiansen

{"title":"Permeabilization of enterocytes induced by absorption of dietary fat.","authors":"Erik Michael Danielsen, Gert H Hansen, Karina Rasmussen, Lise-Lotte Niels-Christiansen","doi":"10.3109/09687688.2013.780642","DOIUrl":"https://doi.org/10.3109/09687688.2013.780642","url":null,"abstract":"Absorption of dietary fat in the small intestine involves epithelial exposure to potentially harmful molecules such as bile salts and free fatty acids. We used organ culture of porcine jejunal explants incubated with a pre-digested mixture of fat (plant oil), bile and pancreatin to mimick the physiological process of dietary fat absorption, and short exposures to the fat mixture caused fat droplet accumulation within villus enterocytes. Lucifer yellow (LY), a fluorescent membrane-impermeable polar tracer was included to monitor epithelial integrity. Both in controls and during fat absorption LY penetrated the epithelium and accumulated in the basal lamina and the lamina propria. LY was also seen in the paracellular space, whereas villus enterocytes were generally only weakly labeled except for small amounts taken up by apical endocytosis. In the crypts, however, fat absorption induced cell permeabilization with LY accumulating in the cytosol and nucleus. Morphologically, both apical and basolateral membranes appeared intact, indicating that the leakiness was caused by minor lesions in the membrane. Albeit to a lesser extent, bile alone was capable of permeabilizing crypt cells, implying that the surfactant properties of bile salts are involved in the process. In addition to LY, crypt enterocytes also became permeable for albumin, ovalbumin and insulin. In conclusion, during fat absorption the permeability of the gut epithelium is increased mainly in the crypts. A possible explanation is that cell membranes of immature crypt cells, lacking detergent-resistant lipid raft microdomains, are less resistant to the deleterious effects of bile salts and free fatty acids.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"30 3","pages":"261-72"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2013.780642","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31426661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15