Molecular Membrane Biology最新文献_第2页

Combining genetic and biophysical approaches to probe the structure and function relationships of the notch receptor. 结合遗传学和生物物理学方法探讨notch受体的结构和功能关系。

Molecular Membrane Biology Pub Date : 2017-02-01 Epub Date: 2018-09-24 DOI: 10.1080/09687688.2018.1503742

Martin Baron

{"title":"Combining genetic and biophysical approaches to probe the structure and function relationships of the notch receptor.","authors":"Martin Baron","doi":"10.1080/09687688.2018.1503742","DOIUrl":"https://doi.org/10.1080/09687688.2018.1503742","url":null,"abstract":"Notch is a conserved cell signalling receptor regulating many aspects of development and tissue homeostasis. Notch is activated by ligand-induced proteolytic cleavages that release the Notch intracellular domain, which relocates to the nucleus to regulate gene transcription. Proteolytic activation first requires mechanical force to be applied to the Notch extracellular domain through an endocytic pulling mechanism transmitted through the ligand/receptor interface. This exposes the proteolytic cleavage site allowing the signal to be initiated following removal of the Notch extracellular domain. Ligands can also act, when expressed in the same cell, through non-productive cis-interactions to inhibit Notch activity. Furthermore, ligand selectivity and Notch activation are regulated by numerous post-translational modifications of the extracellular domain. Additional non-canonical trans and cis interactions with other regulatory proteins may modulate alternative mechanisms of Notch activation that depend on endocytic trafficking of the full-length receptor and proteolytic release of the intracellular domain from endo-lysosomal surface. Mutations of Notch, located in different regions of the protein, are associated with a spectrum of different loss and gain of function phenotypes and offer the possibility to dissect distinct regulatory interactions and mechanisms, particularly when combined with detailed structural analysis of Notch in complex with various regulatory partners.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"34 1-2","pages":"33-49"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2018.1503742","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36516688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Adaptor protein 1 B mu subunit does not contribute to the recycling of kAE1 protein in polarized renal epithelial cells. 接头蛋白1b μ亚基在极化肾上皮细胞中不参与kAE1蛋白的再循环。

Molecular Membrane Biology Pub Date : 2017-02-01 Epub Date: 2018-04-13 DOI: 10.1080/09687688.2018.1451662

Ensaf Y Almomani, Nicolas Touret, Emmanuelle Cordat

{"title":"Adaptor protein 1 B mu subunit does not contribute to the recycling of kAE1 protein in polarized renal epithelial cells.","authors":"Ensaf Y Almomani, Nicolas Touret, Emmanuelle Cordat","doi":"10.1080/09687688.2018.1451662","DOIUrl":"https://doi.org/10.1080/09687688.2018.1451662","url":null,"abstract":"Mutations in the gene encoding the kidney anion exchanger 1 (kAE1) can lead to distal renal tubular acidosis (dRTA). dRTA mutations reported within the carboxyl (C)-terminal tail of kAE1 result in apical mis-targeting of the exchanger in polarized renal epithelial cells. As kAE1 physically interacts with the μ subunit of epithelial adaptor protein 1 B (AP-1B), we investigated the role of heterologously expressed μ1B subunit of the AP-1B complex for kAE1 retention to the basolateral membrane in polarized porcine LLC-PK1 renal epithelial cells that are devoid of endogenous AP-1B. We confirmed the interaction and close proximity between kAE1 and μ1B using immunoprecipitation and proximity ligation assay, respectively. Expressing the human μ1B subunit in these cells decreased significantly the amount of cell surface kAE1 at the steady state, but had no significant effect on kAE1 recycling and endocytosis. We show that (i) heterologous expression of μ1B displaces the physical interaction of endogenous GAPDH with kAE1 WT supporting that both AP-1B and GAPDH proteins bind to an overlapping site on kAE1 and (ii) phosphorylation of tyrosine 904 within the potential YDEV interaction motif does not alter the kAE1/AP-1B interaction. We conclude that μ1B subunit is not involved in recycling of kAE1.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"34 1-2","pages":"50-64"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2018.1451662","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36007037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Structural biology of solute carrier (SLC) membrane transport proteins. 溶质载体(SLC)膜转运蛋白的结构生物学。

Molecular Membrane Biology Pub Date : 2017-02-01 Epub Date: 2018-04-13 DOI: 10.1080/09687688.2018.1448123

Xiaoyun Bai, Trevor F Moraes, Reinhart A F Reithmeier

{"title":"Structural biology of solute carrier (SLC) membrane transport proteins.","authors":"Xiaoyun Bai, Trevor F Moraes, Reinhart A F Reithmeier","doi":"10.1080/09687688.2018.1448123","DOIUrl":"https://doi.org/10.1080/09687688.2018.1448123","url":null,"abstract":"Abstract The human solute carriers (SLCs) comprise over 400 different transporters, organized into 65 families (http://slc.bioparadigms.org/) based on their sequence homology and transport function. SLCs are responsible for transporting extraordinarily diverse solutes across biological membranes, including inorganic ions, amino acids, lipids, sugars, neurotransmitters and drugs. Most of these membrane proteins function as coupled symporters (co-transporters) utilizing downhill ion (H+ or Na+) gradients as the driving force for the transport of substrate against its concentration gradient into cells. Other members work as antiporters (exchangers) that typically contain a single substrate-binding site with an alternating access mode of transport, while a few members exhibit channel-like properties. Dysfunction of SLCs is correlated with numerous human diseases and therefore they are potential therapeutic drug targets. In this review, we identified all of the SLC crystal structures that have been determined, most of which are from prokaryotic species. We further sorted all the SLC structures into four main groups with different protein folds and further discuss the well-characterized MFS (major facilitator superfamily) and LeuT (leucine transporter) folds. This review provides a systematic analysis of the structure, molecular basis of substrate recognition and mechanism of action in different SLC family members.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"34 1-2","pages":"1-32"},"PeriodicalIF":0.0,"publicationDate":"2017-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2018.1448123","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36009165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 112

Mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation. NF-κB信号通路和自噬调节成骨细胞分化的机制。

Molecular Membrane Biology Pub Date : 2016-09-01 Epub Date: 2017-11-23 DOI: 10.1080/09687688.2017.1400601

Han Qin, Hong-Zhi Xu, Yong-Qing Gong

{"title":"Mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation.","authors":"Han Qin, Hong-Zhi Xu, Yong-Qing Gong","doi":"10.1080/09687688.2017.1400601","DOIUrl":"https://doi.org/10.1080/09687688.2017.1400601","url":null,"abstract":"Objective: The objective of the present work was to investigate a possible mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation, and provide experimental basis for the study of tooth eruption disorder.Methods: Mouse osteoblast-like (MC3T3-E1) cells were inoculated with a cell density of 70%. According to the grouping experimental design, Western blot and monodansylcadaverine (MDC) detection were conducted after dosing for 24 h. The cells were divided into the following five groups: blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group; 25 µg/mL SN50 group and 50 µg/mL SN50 group.Results: Western blot analysis revealed that the expression of LC3 protein was present in the blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group and 50 µg/mL SN50 group, with no significant differences among these groups. However, the expression of LC3 protein was significantly lower in the 25 µg/mL SN50 group. MDC detection showed that, in the blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group and 50 µg/mL SN50 group, there was obvious green fluorescence in the cytoplasm of the osteoblasts. However, in the 25 µg/mL SN50 group, it was found that there were significantly fewer green fluorescent particles.Conclusion: The osteoblast itself had a strong function of autophagy. The appropriate concentration of SN50 in blocking the NF-κB pathway of the osteoblast was associated with the obvious inhibition of autophagy. However, the relationship between NF-κB signaling pathway and autophagy in the process of tooth eruption requires further study.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 6-8","pages":"138-144"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1400601","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35635519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Regulation of stressed-induced cell death by the Bcl-2 family of apoptotic proteins. 凋亡蛋白Bcl-2家族调控应激诱导的细胞死亡。

Molecular Membrane Biology Pub Date : 2016-09-01 Epub Date: 2017-11-23 DOI: 10.1080/09687688.2017.1400600

Rabih Roufayel

引用次数: 25

The octanoylated energy regulating hormone ghrelin: An expanded view of ghrelin's biological interactions and avenues for controlling ghrelin signaling. 辛烷酰化的能量调节激素胃饥饿素:对胃饥饿素生物相互作用和控制胃饥饿素信号传导途径的扩展观点。

Molecular Membrane Biology Pub Date : 2016-09-01 Epub Date: 2017-11-16 DOI: 10.1080/09687688.2017.1388930

Elizabeth R Cleverdon, Kayleigh R McGovern-Gooch, James L Hougland

{"title":"The octanoylated energy regulating hormone ghrelin: An expanded view of ghrelin's biological interactions and avenues for controlling ghrelin signaling.","authors":"Elizabeth R Cleverdon, Kayleigh R McGovern-Gooch, James L Hougland","doi":"10.1080/09687688.2017.1388930","DOIUrl":"https://doi.org/10.1080/09687688.2017.1388930","url":null,"abstract":"Ghrelin is a small peptide hormone that requires a unique post-translational modification, serine octanoylation, to bind and activate the GHS-R1a receptor. Initially demonstrated to stimulate hunger and appetite, ghrelin-dependent signaling is implicated in a variety of neurological and physiological processes influencing diseases such as diabetes, obesity, and Prader-Willi syndrome. In addition to its cognate receptor, recent studies have revealed ghrelin interacts with a range of binding partners within the bloodstream. Defining the scope of ghrelin's interactions within the body, understanding how these interactions work in concert to modulate ghrelin signaling, and developing molecular tools for controlling ghrelin signaling are essential for exploiting ghrelin for therapeutic effect. In this review, we discuss recent findings regarding the biological effects of ghrelin signaling, outline binding partners that control ghrelin trafficking and stability in circulation, and summarize the current landscape of inhibitors targeting ghrelin octanoylation.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 6-8","pages":"111-124"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1388930","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35254167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Bacterial outer membrane vesicles: New insights and applications. 细菌外膜囊泡:新的见解和应用。

Molecular Membrane Biology Pub Date : 2016-09-01 DOI: 10.1080/09687688.2017.1400602

Deepak Anand, Arunima Chaudhuri

引用次数: 38

K⁺-Cl^- co-transporter 2 (KCC2) - a membrane trafficking perspective. K+- cl -共转运蛋白2 (KCC2) -膜运输的视角。

Molecular Membrane Biology Pub Date : 2016-09-01 Epub Date: 2017-11-16 DOI: 10.1080/09687688.2017.1393566

Bor Luen Tang

{"title":"K+-Cl- co-transporter 2 (KCC2) - a membrane trafficking perspective.","authors":"Bor Luen Tang","doi":"10.1080/09687688.2017.1393566","DOIUrl":"https://doi.org/10.1080/09687688.2017.1393566","url":null,"abstract":"K+-Cl- co-transporter 2 (KCC2/SLC12A5) is a neuronal specific cation chloride co-transporter which is active under isotonic conditions, and thus a key regulator of intracellular Cl- levels. It also has an ion transporter-independent structural role in modulating the maturation and regulation of excitatory glutamatergic synapses. KCC2 levels are developmentally regulated, and a postnatal upregulation of KCC2 generates a low intracellular chloride concentration that allows the neurotransmitters γ-aminobutyric acid (GABA) and glycine to exert inhibitory neurotransmission through its Cl- permeating channel. Functional expression of KCC2 at the neuronal cell surface is necessary for its activity, and impairment in KCC2 cell surface transport and/or internalization may underlie a range of neuropathological conditions. Although recent advances have shed light on a range of cellular mechanisms regulating KCC2 activity, little is known about its membrane trafficking itinerary and regulatory proteins. In this review, known membrane trafficking signals, pathways and mechanisms pertaining to KCC2's functional surface expression are discussed.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 6-8","pages":"100-110"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1393566","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35614971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

pH-sensitive pHLIP^® coated niosomes. ph敏感的pHLIP®包被乳质体。

Molecular Membrane Biology Pub Date : 2016-05-01 Epub Date: 2017-08-09 DOI: 10.1080/09687688.2017.1342969

Mohan C Pereira, Monica Pianella, Da Wei, Anna Moshnikova, Carlotta Marianecci, Maria Carafa, Oleg A Andreev, Yana K Reshetnyak

{"title":"pH-sensitive pHLIP® coated niosomes.","authors":"Mohan C Pereira, Monica Pianella, Da Wei, Anna Moshnikova, Carlotta Marianecci, Maria Carafa, Oleg A Andreev, Yana K Reshetnyak","doi":"10.1080/09687688.2017.1342969","DOIUrl":"https://doi.org/10.1080/09687688.2017.1342969","url":null,"abstract":"Nanomedicine is becoming very popular over conventional methods due to the ability to tune physico-chemical properties of nano vectors, which are used for encapsulation of therapeutic and diagnostic agents. However, the success of nanomedicine primarily relies on how specifically and efficiently nanocarriers can target pathological sites to minimize undesirable side effects and enhance therapeutic efficacy. Here, we introduce a novel class of targeted nano drug delivery system, which can be used as an effective nano-theranostic for cancer. We formulated pH-sensitive niosomes (80-90 nm in diameter) using nonionic surfactants Span20 (43-45 mol%), cholesterol (50 mol%) and 5 mol% of pH (Low) insertion peptide (pHLIP) conjugated with DSPE lipids (DSPE-pHLIP) or hydrophobic fluorescent dye, pyrene, (Pyr-pHLIP). In coating of niosomes, pHLIP was used as an acidity sensitive targeting moiety. We have demonstrated that pHLIP coated niosomes sense the extracellular acidity of cancerous cells. Intravenous injection of fluorescently labeled (R18) pHLIP-coated niosomes into mice bearing tumors showed significant accumulation in tumors with minimal targeting of kidney, liver and muscles. Tumor-targeting niosomes coated with pHLIP exhibited 2-3 times higher tumor uptake compared to the non-targeted niosomes coated with PEG polymer. Long circulation time and uniform bio-distribution throughout the entire tumor make pHLIP-coated niosomes to be an attractive novel delivery system.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 3-5","pages":"51-63"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1342969","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35396192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Curcumin inhibits oxidative stress-induced TRPM2 channel activation, calcium ion entry and apoptosis values in SH-SY5Y neuroblastoma cells: Involvement of transfection procedure. 姜黄素抑制SH-SY5Y神经母细胞瘤细胞氧化应激诱导的TRPM2通道激活、钙离子进入和凋亡值:转染过程的参与

Molecular Membrane Biology Pub Date : 2016-05-01 Epub Date: 2017-06-01 DOI: 10.1080/09687688.2017.1318224

Ahmi Öz, Ömer Çelik

{"title":"Curcumin inhibits oxidative stress-induced TRPM2 channel activation, calcium ion entry and apoptosis values in SH-SY5Y neuroblastoma cells: Involvement of transfection procedure.","authors":"Ahmi Öz, Ömer Çelik","doi":"10.1080/09687688.2017.1318224","DOIUrl":"https://doi.org/10.1080/09687688.2017.1318224","url":null,"abstract":"Transient Receptor Potential (TRP) channels are mostly Ca2+ permeable cation channels. Transient Receptor Potential Melastatin-like 2 (TRPM2) is expressed in neurological tissues such as brain, dorsal root ganglia (DRG) neurons, hippocampus and also liver, heart and kidney. The SH-SY5Y cells are mostly used as a cellular model of neurodegenerative diseases, Alzheimer's and Parkinson's diseases. Curcumin, shows phenolic structure, synthesized by Curcuma longa L. (turmeric), has powerful non-enzymatically antioxidant effects compared with Vitamin E. Hence, we aimed to investigate that effects of curcumin on TRPM2 cation channel currents using the whole-cell Patch-Clamp method, Ca2+ signaling, apoptosis and cell viability (MTT) assays, reactive oxygen species (ROS) production, mitochondrial membrane potential levels, caspase 3 and caspase 9 activities in TRPM2 transfected SH-SY5Y neuroblastoma cells. For this aim, we designed four experimental groups named; control, curcumin, transfected and transfected + curcumin groups. Cytosolic free calcium concentrations were higher in transfected group compared with curcumin and transfected + curcumin group. Moreover, these data examined with whole-cell Patch-Clamp recordings of single cells in all groups. ROS levels were significantly higher in transfected group than in transfected + curcumin group. Apoptosis levels in transfected + curcumin group were lower than in transfected group. Procaspase 9 and procaspase 3 levels measured by western blotting and caspase 3 and caspase 9 levels by spectrophotometric methods show that TRPM2 transfected cells are more tended to apoptosis. In conclusion, curcumin strongly induces modulator effects on TRPM2-mediated Ca2+ influx caused by ROS and caspase 3 and 9 processes in SH-SY5Y neuroblastoma cells.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 3-5","pages":"76-88"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1318224","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35051663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 51