Molecular Membrane Biology最新文献_第3页

Membrane-bound pyrophosphatase of human gut microbe Clostridium methylpentosum confers improved salt tolerance in Escherichia coli, Saccharomyces cerevisiae and tobacco. 人肠道微生物甲基戊酸梭菌的膜结合焦磷酸酶提高了大肠杆菌、酿酒酵母和烟草的耐盐性。

Molecular Membrane Biology Pub Date : 2016-05-01 DOI: 10.1080/09687688.2017.1370145

Yumei Yang, Yanjuan Liu, Hang Yuan, Xian Liu, Yanxiu Gao, Ming Gong, Zhurong Zou

{"title":"Membrane-bound pyrophosphatase of human gut microbe Clostridium methylpentosum confers improved salt tolerance in Escherichia coli, Saccharomyces cerevisiae and tobacco.","authors":"Yumei Yang, Yanjuan Liu, Hang Yuan, Xian Liu, Yanxiu Gao, Ming Gong, Zhurong Zou","doi":"10.1080/09687688.2017.1370145","DOIUrl":"https://doi.org/10.1080/09687688.2017.1370145","url":null,"abstract":"Membrane-bound pyrophosphatases (PPases) are involved in the adaption of organisms to stress conditions, which was substantiated by numerous plant transgenic studies with H+-PPase yet devoid of any correlated evidences for other two subfamilies, Na+-PPase and Na+,H+-PPase. Herein, we demonstrate the gene cloning and functional evaluation of the membrane-bound PPase (CmPP) of the human gut microbe Clostridium methylpentosum. The CmPP gene encodes a single polypeptide of 699 amino acids that was predicted as a multi-spanning membrane and K+-dependent Na+,H+-PPase. Heterologous expression of CmPP could significantly enhance the salt tolerance of both Escherichia coli and Saccharomyces cerevisiae, and this effect in yeast could be fortified by N-terminal addition of a vacuole-targeting signal peptide from the H+-PPase of Trypanosoma cruzi. Furthermore, introduction of CmPP could remarkably improve the salt tolerance of tobacco, implying its potential use in constructing salt-resistant transgenic crops. Consequently, the possible mechanisms of CmPP to underlie salt tolerance are discussed.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 3-5","pages":"39-50"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1370145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35444984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The partition and transport behavior of cytotoxic ionic liquids (ILs) through the DPPC bilayer: Insights from molecular dynamics simulation. 细胞毒性离子液体(ILs)通过DPPC双分子层的分配和运输行为:来自分子动力学模拟的见解。

Molecular Membrane Biology Pub Date : 2016-05-01 DOI: 10.1080/09687688.2017.1384859

Mokhtar Ganjali Koli, Khaled Azizi

{"title":"The partition and transport behavior of cytotoxic ionic liquids (ILs) through the DPPC bilayer: Insights from molecular dynamics simulation.","authors":"Mokhtar Ganjali Koli, Khaled Azizi","doi":"10.1080/09687688.2017.1384859","DOIUrl":"https://doi.org/10.1080/09687688.2017.1384859","url":null,"abstract":"A molecular dynamics (MD) simulation with atomistic details was performed to examine the partitioning and transport behavior of moderately cytotoxic ionic liquids (ILs), namely choline bis(2-ethylhexyl) phosphate (CBEH), choline bis(2,4,4-trimethylpentyl) phosphinate (CTMP) and choline O,O-diethyl dithiophosphate (CDEP) in a fully hydrated dipalmitoylphosphatidylcholine (DPPC) bilayer in the fluid phase at 323 K. The structure of ILs was so selected to understand if the role of dipole and dispersion forces in the ILs distribution in the membrane can be possible. Several analyses including mass density, electrostatic potential, order parameter, diffusion coefficients and hydrogen bond formation, was carried out to determine the precise location of the anionic species inside the membrane. Moreover, the potential of the mean force (PMF) method was used to calculate free energy profile for transferring anionic species from the DPPC membrane into the bulk water. While less cytotoxic DEP is located within the bulk water, more cytotoxic TMP and BEH ILs were found to remain in the membrane and the energy barrier for crossing through the bilayer center of BEH was higher. Various ILs have no significant effect on P-N vector. The thickness of lipid bilayer decreased in all systems comprising ILs, while area per lipid increased.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 3-5","pages":"64-75"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2017.1384859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35444986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

The changing landscape of membrane protein structural biology through developments in electron microscopy. 电子显微镜的发展改变了膜蛋白结构生物学的面貌。

Molecular Membrane Biology Pub Date : 2016-03-01 Epub Date: 2016-09-09 DOI: 10.1080/09687688.2016.1221533

Shaun Rawson, Simon Davies, Jonathan D Lippiat, Stephen P Muench

引用次数: 0

Molecular mechanisms linking geranylgeranyl diphosphate synthase to cell survival and proliferation. 香叶二磷酸合酶与细胞存活和增殖的分子机制。

Molecular Membrane Biology Pub Date : 2016-03-01 Epub Date: 2016-08-18 DOI: 10.1080/09687688.2016.1213432

Sherry S Agabiti, Yilan Liang, Andrew J Wiemer

引用次数: 17

The influence of NBD fluorescent probe on model membranes containing POPC and DPPC. NBD荧光探针对含POPC和DPPC模型膜的影响。

Molecular Membrane Biology Pub Date : 2016-03-01 Epub Date: 2016-07-25 DOI: 10.1080/09687688.2016.1185175

Chi-Jung Weng, Ju-Ping Wu, Ming-Yen Kuo, Ya-Wei Hsueh

{"title":"The influence of NBD fluorescent probe on model membranes containing POPC and DPPC.","authors":"Chi-Jung Weng, Ju-Ping Wu, Ming-Yen Kuo, Ya-Wei Hsueh","doi":"10.1080/09687688.2016.1185175","DOIUrl":"https://doi.org/10.1080/09687688.2016.1185175","url":null,"abstract":"To investigate the effect of fluorescent probe on the properties of membranes, we studied model membranes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl 2-oleoyl-sn-glycero-3-phosphocholine (POPC) in the presence and absence of fluorescent probe. The morphology of giant unilamellar vesicles (GUVs) has been observed as a function of temperature and composition by fluorescence microscopy using NBD-DOPE or C6-NBD-PC as the probe. The phase behavior of model membranes containing no fluorescent probe was investigated by 2H-NMR spectroscopy. We found that the bright phase observed on GUVs was the fluid phase enriched in POPC and the dark phase was the gel phase enriched in DPPC. NBD-DOPE and C6-NBD-PC preferentially participated in the fluid-phase domains when GUVs were in the gel + fluid phase coexistence. Inclusion of both fluorescent probes (1 mol%) lowered the transition temperature of POPC/DPPC membranes. In addition, C6-NBD-PC exhibited a stronger effect than NBD-DOPE, which was considered to be associated with the structures of fluorescent molecules.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"33 1-2","pages":"23-28"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09687688.2016.1185175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34700293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Secretome derived from breast tumor cell lines alters the morphology of human umbilical vein endothelial cells 来源于乳腺肿瘤细胞系的分泌组改变了人脐静脉内皮细胞的形态

Molecular Membrane Biology Pub Date : 2016-02-17 DOI: 10.1080/09687688.2016.1229057

E. O. Gómez, Y. Chirino, N. Delgado-Buenrostro, Alejandro López-Saavedra, N. Meraz-Cruz, R. López-Marure

引用次数: 3

Electrostatic networks control plug stabilization in the PapC usher. 静电网络控制插头稳定在PapC迎来。

Molecular Membrane Biology Pub Date : 2015-08-01 Epub Date: 2016-05-16 DOI: 10.3109/09687688.2016.1160450

Thieng Pham, Nadine S Henderson, Glenn T Werneburg, David G Thanassi, Anne H Delcour

{"title":"Electrostatic networks control plug stabilization in the PapC usher.","authors":"Thieng Pham, Nadine S Henderson, Glenn T Werneburg, David G Thanassi, Anne H Delcour","doi":"10.3109/09687688.2016.1160450","DOIUrl":"https://doi.org/10.3109/09687688.2016.1160450","url":null,"abstract":"The PapC usher, a β-barrel pore in the outer membrane of uropathogenic Escherichia coli, is used for assembly of the P pilus, a key virulence factor in bacterial colonization of human kidney cells. Each PapC protein is composed of a 24-stranded β-barrel channel, flanked by N- and C-terminal globular domains protruding into the periplasm, and occluded by a plug domain (PD). The PD is displaced from the channel towards the periplasm during pilus biogenesis, but the molecular mechanism for PD displacement remains unclear. Two structural features within the β-barrel, an α-helix and β5-6 hairpin loop, may play roles in controlling plug stabilization. Here we have tested clusters of residues at the interface of the plug, barrel, α-helix and hairpin, which participate in electrostatic networks. To assess the roles of these residues in plug stabilization, we used patch-clamp electrophysiology to compare the activity of wild-type and mutant PapC channels containing alanine substitutions at these sites. Mutations interrupting each of two salt bridge networks were relatively ineffective in disrupting plug stabilization. However, mutation of two pairs of arginines located at the inner and the outer surfaces of the PD resulted in an enhanced propensity for plug displacement. One arginine pair involved in a repulsive interaction between the linkers that tether the plug to the β-barrel was particularly sensitive to mutation. These results suggest that plug displacement, which is necessary for pilus assembly and translocation, may require a weakening of key electrostatic interactions between the plug linkers, and the plug and the α-helix.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"32 5-8","pages":"198-207"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2016.1160450","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34550399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Solid-state NMR structures of integral membrane proteins 整体膜蛋白的固态核磁共振结构

Molecular Membrane Biology Pub Date : 2015-08-01 DOI: 10.3109/09687688.2016.1139754

S. G. Patching

{"title":"Solid-state NMR structures of integral membrane proteins","authors":"S. G. Patching","doi":"10.3109/09687688.2016.1139754","DOIUrl":"https://doi.org/10.3109/09687688.2016.1139754","url":null,"abstract":"Abstract Solid-state NMR is unique for its ability to obtain three-dimensional structures and to measure atomic-resolution structural and dynamic information for membrane proteins in native lipid bilayers. An increasing number and complexity of integral membrane protein structures have been determined by solid-state NMR using two main methods. Oriented sample solid-state NMR uses macroscopically aligned lipid bilayers to obtain orientational restraints that define secondary structure and global fold of embedded peptides and proteins and their orientation and topology in lipid bilayers. Magic angle spinning (MAS) solid-state NMR uses unoriented rapidly spinning samples to obtain distance and torsion angle restraints that define tertiary structure and helix packing arrangements. Details of all current protein structures are described, highlighting developments in experimental strategy and other technological advancements. Some structures originate from combining solid- and solution-state NMR information and some have used solid-state NMR to refine X-ray crystal structures. Solid-state NMR has also validated the structures of proteins determined in different membrane mimetics by solution-state NMR and X-ray crystallography and is therefore complementary to other structural biology techniques. By continuing efforts in identifying membrane protein targets and developing expression, isotope labelling and sample preparation strategies, probe technology, NMR experiments, calculation and modelling methods and combination with other techniques, it should be feasible to determine the structures of many more membrane proteins of biological and biomedical importance using solid-state NMR. This will provide three-dimensional structures and atomic-resolution structural information for characterising ligand and drug interactions, dynamics and molecular mechanisms of membrane proteins under physiological lipid bilayer conditions.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"32 1","pages":"156 - 178"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2016.1139754","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69392285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

The influence of an antitumor lipid – erucylphosphocholine – on artificial lipid raft system modeled as Langmuir monolayer 抗肿瘤脂质-乙酰胆碱-对Langmuir单层人工脂筏系统的影响

Molecular Membrane Biology Pub Date : 2015-08-01 DOI: 10.3109/09687688.2015.1125537

Anita Wnętrzak, K. Łat̀ka, Katarzyna Makyła-Juzak, J. Zemła, P. Dynarowicz-Łątka

{"title":"The influence of an antitumor lipid – erucylphosphocholine – on artificial lipid raft system modeled as Langmuir monolayer","authors":"Anita Wnętrzak, K. Łat̀ka, Katarzyna Makyła-Juzak, J. Zemła, P. Dynarowicz-Łątka","doi":"10.3109/09687688.2015.1125537","DOIUrl":"https://doi.org/10.3109/09687688.2015.1125537","url":null,"abstract":"Abstract Outer layer of cellular membrane contains ordered domains enriched in cholesterol and sphingolipids, called ‘lipid rafts’, which play various biological roles, i.e., are involved in the induction of cell death by apoptosis. Recent studies have shown that these domains may constitute binding sites for selected drugs. For example alkylphosphocholines (APCs), which are new-generation antitumor agents characterized by high selectivity and broad spectrum of activity, are known to have their molecular targets located at cellular membrane and their selective accumulation in tumor cells has been hypothesized to be linked with the alternation of biophysical properties of lipid rafts. To get a deeper insight into this issue, interactions between representative APC: erucylphosphocholine, and artificial lipid raft system, modeled as Langmuir monolayer (composed of cholesterol and sphingomyelin mixed in 1:2 proportion) were investigated. The Langmuir monolayer experiments, based on recording surface pressure-area isotherms, were complemented with Brewster angle microscopy results, which enabled direct visualization of the monolayers structure. In addition, the investigated monolayers were transferred onto solid supports and studied with AFM. The interactions between model raft system and erucylphosphocholine were analyzed qualitatively (with mean molecular area values) as well as quantitatively (with ΔGexc function). The obtained results indicate that erucylphosphocholine introduced to raft-mimicking model membrane causes fluidizing effect and weakens the interactions between cholesterol and sphingomyelin, which results in phase separation at high surface pressures. This leads to the redistribution of cholesterol molecules in model raft, which confirms the results observed in biological studies.","PeriodicalId":18858,"journal":{"name":"Molecular Membrane Biology","volume":"32 1","pages":"189 - 197"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/09687688.2015.1125537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69392279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Slippery signaling: Palmitoylation-dependent control of neuronal kinase localization and activity. 滑溜信号:棕榈酰化依赖的神经元激酶定位和活性控制。

Molecular Membrane Biology Pub Date : 2015-08-01 Epub Date: 2016-05-31 DOI: 10.1080/09687688.2016.1182652

Audrey Montersino, Gareth M Thomas

引用次数: 21