Molecular Reproduction and Development最新文献

{"title":"Transcriptional Profiling of Cumulus Cells From FLI-Matured Porcine Oocytes Identifies Junctional Genes as Key Components in Oocyte Maturation","authors":"Caroline L. Green,&nbsp;Aleah Jaworski,&nbsp;Paula Mangiavacchi,&nbsp;Lee Spate,&nbsp;Lianna Walker,&nbsp;Randall S. Prather,&nbsp;Kiho Lee,&nbsp;Bethany K. Redel","doi":"10.1002/mrd.70080","DOIUrl":"10.1002/mrd.70080","url":null,"abstract":"<div>\u0000 \u0000 <p>In vitro oocyte maturation and embryo culture are critical for preserving and expanding genetic lines, and are necessary to produce genetically engineered pigs for biomedical and agricultural purposes. However, suboptimal in vitro conditions compromise oocyte and embryo viability. Recent improvements to in vitro maturation medium, including the addition of FGF2, LIF, and IGF1 (FLI), have increased the number of oocytes that reached the metaphase II stage, doubled the number of oocytes that reached the blastocyst stage, and quadrupled the number of piglets born after embryo transfer. Despite these benefits, the underlying cellular mechanisms remain not fully understood. Given the essential role of cumulus cells (CCs) in oocyte maturation, we investigated how FLI affects CCs gene expression. Cumulus-oocyte complexes were matured for 24 h in control or FLI media, and CCs from oocytes that reached the blastocyst stage underwent RNA sequencing. FLI altered 1257 transcripts (423 upregulated, 834 downregulated; adj-<i>p</i> &lt; 0.05), with enrichment of junctional communication genes and downregulation of extracellular matrix organization. Immunofluorescence confirmed increased TJP1, TJP2, and GJA4 in FLI-treated complexes. Additionally, cortical granule localization suggested enhanced cytoplasmic maturation with FLI supplementation. These findings indicate that FLI promotes CC communication and supports improved oocyte competence in porcine in vitro maturation.</p>\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gynogenesis of Pengba Fish Osteobrama belangeri (Valenciennes, 1844): Comparative Analysis on Use of Conspecific and Heterospecific UV-Irradiated Sperm","authors":"Yambem Suresh Singh,&nbsp;Shubham Kashyap,&nbsp;Arun Bhai Patel,&nbsp;Kizhakke Veettil Radhakrishnan,&nbsp;Pramod Kumar Pandey,&nbsp;Himanshu Priyadarshi","doi":"10.1002/mrd.70078","DOIUrl":"10.1002/mrd.70078","url":null,"abstract":"<div>\u0000 \u0000 <p>Gynogenesis has wide application in genetic improvement program for the generation of inbred line, mapping population, monosex population, and conservation. In the present study, embryonic development in gynogenesis of Pengba (<i>Osteobrama belangeri</i> [<i>O. belangeri</i>]) was analyzed to evaluate the efficacy of using conspecific and heterospecific sperm. For the selection of suitable sperm donor, three heterospecific males (Tilapia, Rohu, and Java barb) were experimented with no UV irradiation and thermal shock. The result showed high fertilization rate in case of Rohu and Java barb thus, they were omitted from further gynogenesis, in view of possibilities of producing hybrid offspring. For gynogenesis study, three experiments were conducted; first, conspecific as well as heterospecific sperm (Tilapia) were used to fertilize Pengba eggs (UV-irradiated sperm of Pengba and thermal shock to maintain diploidy) and the fertilization, hatching and survivability rates were recorded as 18%–20%, 5%–6%, and 2%–3%, respectively. Fertilization rate in Tilapia sperm without UV irradiation and thermal shock and UV-irradiated sperm of tilapia and thermal shock to maintain diploidy experiments was recorded as 4%–5%. However, hatching did not occur in either of the cases. Our results form the first report of gynogenesis in <i>O. belangeri</i> using conspecific and heterospecific sperm, which will be helpful in its genetic stock improvement programme and ensure conservation of native stock as well as better production in aquaculture.</p>\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of the Hippo Signaling Pathway in 7,12-Dimethylbenz(a)anthracene (DMBA)-Induced Follicular Depletion","authors":"Serra Ersoy,&nbsp;Aylin Yaba","doi":"10.1002/mrd.70081","DOIUrl":"10.1002/mrd.70081","url":null,"abstract":"<div>\u0000 \u0000 <p>7,12-Dimethylbenz(a)anthracene (DMBA) is a polycyclic aromatic hydrocarbon formed by the combustion of organic substances and has ovotoxic and carcinogenic effects on ovarian follicular development in rodents. The Hippo signaling pathway contributes to the understanding of various molecular mechanisms, including cell proliferation, differentiation, apoptosis, organ size regulation, and tumorigenesis, as an evolutionarily conserved pathway. In this study, we hypothesized that the Hippo signaling pathway may play a role in the mechanism of DMBA-induced ovotoxicity. We aimed to identify Hippo signaling pathway proteins in DMBA-treated ovaries via immunohistochemistry and quantitative real-time PCR (qRT–PCR). Twenty-eight-day-old 18 BalbC female mice were used and divided into three groups. The control group received no treatment, the vehicle group was injected daily with sesame oil, and the DMBA group was injected intraperitoneally with 1 mg/kg/day DMBA (dissolved in sesame oil) for 14 consecutive days. The sections were subjected to periodic acid–Schiff (PAS) staining to demonstrate the morphological differences between the DMBA and control groups of mouse ovaries. Anti-Mullerian hormone (AMH) serum levels were analyzed via ELISA, and follicles were counted to evaluate the follicle reserve. Immunohistochemistry was performed to determine the localization of the Hippo signaling pathway proteins MST1/2, LATS1/2, YAP1, and TEAD4 and to assess oxidative stress with a nitrotyrosine (NTY) antibody. The mRNA levels of Hippo signaling components were detected via qRT–PCR. The Hippo signaling pathway may play a role in the mechanisms underlying the rapid depletion of follicles through increased oxidative stress, an increased number of atretic follicles, and a decreased number of corpus luteum in DMBA-induced ovotoxicity. We demonstrated that in the DMBA-induced ovotoxicity model, the Hippo signaling pathway is inactivated by YAP/TAZ translocation to the nucleus, and the increase in YAP1 and TEAD4 expression is at the translational level. This study provides the first evidence of the relationship between ovotoxicity and the Hippo signaling pathway in DMBA-induced ovotoxicity in mice. Therefore, our findings suggest that the Hippo pathway could be pharmacologically targeted to regulate DMBA-induced ovotoxicity.</p></div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Reproduction and Development: Building on a 45-Year Legacy","authors":"Ivan Cunha Bustamante-Filho","doi":"10.1002/mrd.70079","DOIUrl":"10.1002/mrd.70079","url":null,"abstract":"<p>Effective January 2025, it is with great honor and excitement that I have assumed the role of Editor-in-Chief for Molecular Reproduction and Development (MRD). This marks an important new chapter for the journal, and I am deeply committed to building on its strong reputation within the scientific community</p><p>First, I want to sincerely thank Professor Harvey Florman. He led MRD with incredible dedication and vision. Prof. Florman always championed scientific excellence, making sure our journal maintained its high standards. His deep commitment to top-notch reproductive biology research was key to making MRD a go-to place for cutting-edge work. Under his leadership, MRD didn't just report on the field; it helped shape it, consistently publishing discoveries that moved our understanding of gamete biology and early development forward. His contributions have had a lasting impact, and we're truly grateful.</p><p>Looking ahead, our goal stays the same: to keep MRD a top journal known for its quality and rigor. We'll continue to make sure MRD plays a big role in advancing reproductive biology, serving as an important place for high-quality, peer-reviewed research. This commitment follows the path set by our founding editor, Ralph B.L. Gwatkin, who wanted a place for easy information sharing on gametes and early development. It also builds on the strong foundation created by past leaders, including Gary M. Wessel, whose decade as editor expanded the journal's focus and brought the community closer.</p><p>As the new editor, I'll make sure MRD keeps innovating while staying true to its core values. We'll actively look for research that not only improves our basic understanding of reproduction but also covers new areas like genomics, epigenetics, developmental programming, and how environmental factors affect reproductive health. We'll also welcome studies using advanced methods, such as single-cell analysis and advanced imaging, to keep MRD at the leading edge of science. Plus, we'll work on making the journal more accessible and engaging, creating a more inclusive and collaborative space for everyone involved.</p><p>I'm really excited about what's next for Molecular Reproduction and Development. I encourage researchers worldwide to keep sending their important work to our journal. Together, we'll keep pushing the boundaries of reproductive biology, making sure MRD stays a vital resource for scientists for years to come. Thank you for your continued support.</p><p><b>Ivan Cunha Bustamante-Filho:</b> writing – original draft, writing – review and editing.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"93 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mrd.70079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145893014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulation of Chromosome 14 MicroRNA Cluster and Chromosome 19 MicroRNA Cluster MicroRNAs in Chorionic Villous Tissue of Cases With Recurrent Spontaneous Abortion","authors":"Mamata Datar,&nbsp;Vandana Bansal,&nbsp;Padmaja Samant,&nbsp;Kumari Nishi,&nbsp;Nafisa H. Balasinor","doi":"10.1002/mrd.70077","DOIUrl":"10.1002/mrd.70077","url":null,"abstract":"<div>\u0000 \u0000 <p>Recurrent spontaneous abortions (RSA) are defined as a loss of two or more consecutive clinically recognized pregnancies before the 20th week of gestation. In RSA, several causative factors are known, but still, 50% of the cases remain unexplained. Two large clusters of microRNAs (miRNAs), namely C14MC (Chromosome 14 microRNA cluster) and C19MC (Chromosome 19 microRNA cluster), are imprinted and expressed in the placenta. Hence, we studied the expression of miRNAs coded by these clusters and their mRNA targets in RSA cases in order to elucidate the involvement of these two clusters in the pathogenesis of RSA. Upon miRNA sequencing, we found a total of seven miRNAs to be differentially expressed in RSA group. Further, using bioinformatics tools such as TargetScan and DAVID pathway analysis, we found that the mRNA targets were involved in the functionally relevant processes of the placenta such as cell migration, cell–cell adhesion, and angiogenesis. Upon validation of sequencing data, we found differential expression of five miRNAs from C14MC and C19MC clusters and differential expression of genes regulating processes such as cell migration, cell adhesion, and angiogenesis in RSA cases which are targeted by these miRNAs.</p>\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential","authors":"Keith E. Latham","doi":"10.1002/mrd.70072","DOIUrl":"10.1002/mrd.70072","url":null,"abstract":"<p>During recent decades a myriad of approaches have emerged for generating surrogate gametes or achieving in vitro gametogenesis (IVG). The most recent approaches to IVG include the development of increasingly complex culture systems, use of companion cells combined with germ line cells in two- or three-dimensional configurations, derivation of germ line-like cells from pluripotent stem cells, and genetic and chromosomal manipulations of pluripotent stem cells to enable the production of bi-maternal and bi-paternal offspring. This review summarizes the major approaches in IVG, the challenges remaining, and considers the significance of these technologies within the context of departure from exclusively dioecious sexual reproduction for the human species.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12696624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145724734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of CCNK Leads to Early Resumption of Meiosis in Mouse Oocytes","authors":"Yuan-Hong Xu,&nbsp;Si-Min Sun,&nbsp;Bing-Wang Zhao,&nbsp;Jia-Ni Guo,&nbsp;Xue-Mei Yang,&nbsp;Yi-Ke Lu,&nbsp;Yi-Na Zhang,&nbsp;Ji Ding,&nbsp;Zhen-Bo Wang","doi":"10.1002/mrd.70075","DOIUrl":"10.1002/mrd.70075","url":null,"abstract":"<div>\u0000 \u0000 <p>The synthesis and degradation of Cyclins are critical for cell cycle progression. Cyclin K (CCNK), a member of the Cyclin family, is known to regulate transcriptional elongation and the MAPK signaling pathway by binding to CDK12/13. While CCNK has been extensively studied in cancers, its roles in oocyte maturation remains unclear. In this study, we found that overexpression of CCNK accelerates the resumption of meiosis in oocytes. This effect is primarily attributed to the early nuclear entry of Cyclin B1 (CCNB1) and the premature activation of maturation promoting factor. These findings suggest that CCNK plays a vital role in the process of oocyte maturation, and its dysregulation could disrupt the normal progression of meiosis.</p>\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Art1 in Sperm Damage Induced by Type 2 Diabetes Mellitus","authors":"Shumin Xu,&nbsp;Xinyu Yang,&nbsp;Shiyu Pan,&nbsp;Jian Zhang,&nbsp;Qinran Zhu,&nbsp;Xin Li,&nbsp;Ke Hu,&nbsp;Meng Liang,&nbsp;Lei Liu","doi":"10.1002/mrd.70076","DOIUrl":"10.1002/mrd.70076","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetes-associated spermatogenic dysfunction remains mechanistically unclear despite its clinical significance. We aimed to elucidate the effect and molecular mechanism of sperm damage induced by type 2 diabetes mellitus (T2DM). In a mouse model of T2DM, transcriptome sequencing identified <i>Art1</i> as a differentially expressed gene. T2DM induced structural damage to the seminiferous tubules, deregulated sperm parameters, and suppressed <i>Art1</i> expression in mice and caused reproductive damage in their offspring. Danshensu administration rescued these pathologies. <i>In vitro</i>, high-glucose/high-lipid exposure induced GC-2 cell damage, while <i>Art1</i> overexpression reduced oxidative stress and apoptosis. <i>Art1</i>-knockout mice exhibited impaired sperm motility and tubule structural defects, confirming <i>Art1</i>'s critical role in spermatogenesis maintenance. This study demonstrated that T2DM triggers testicular oxidative stress, apoptosis, and <i>Art1</i> suppression, leading to transgenerational reproductive dysfunction. Experiments have shown that the overexpression of Danshensu or <i>Art1</i> can rescue these pathological damages. The results from the <i>Art1</i>-knockout mice confirm the indispensable regulatory role of <i>Art1</i> in spermatogenesis maintenance. We speculate that T2DM may impair male reproductive ability by inhibiting the expression of <i>Art1</i>, which will provide a new molecular target and theoretical basis for further investigation into the interaction mechanism between T2DM and the reproductive system.</p></div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Characterization of Bovine Trophoblast Stem Cells Growing as Adherent Cells or Vesicles","authors":"Camille Baquerre,&nbsp;Apolline Delahaye,&nbsp;Vincent Brochard,&nbsp;Fabienne Nuttinck,&nbsp;Christian Jean,&nbsp;Estelle Robert,&nbsp;Clémence Kress,&nbsp;Sylvie Rival-Gervier,&nbsp;Bertrand Pain,&nbsp;Alice Jouneau","doi":"10.1002/mrd.70073","DOIUrl":"10.1002/mrd.70073","url":null,"abstract":"<p>In ungulates, the development of the trophoblast lineage follows a peculiar process, as implantation is preceded by a relatively long period of trophoblast elongation, the molecular control of which is still not well known. Here, we have established novel bovine trophoblast stem cells (b2iTSCs) growing in a completely defined medium containing inhibitors of GSK3b and MEK, and WNT3 during establishment. These cells can grow either as adherent, or as vesicles in suspension. These fluid-filled vesicles show high proliferative capacity and apical polarity. Transcriptome analysis reveals that b2iTSCs are distinct from bovine embryonic fibroblasts and exhibit gene expression profiles related to lipid metabolism, steroid hormone synthesis, and nutrient absorption. The cells also express different Pregnancy Associated Glycoproteins, such as PAG11. Comparative transcriptome analysis shows that 2iTSCs have a signature intermediate between early trophectoderm and elongating trophoblast, expressing canonical trophoblast markers but not IFNt. Additionally, b2iTSCs can differentiate into binucleate cells in vitro and participate in the formation of bovine blastoïds when cocultured with bovine embryonic stem cells, demonstrating their potential for studying trophoblast development and function.</p>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12683708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"gsdfb, But Not the Isoforms gsdfa1 and gsdfa2, Is Up-Regulated During the Male Program and Repressed by Estrogens in Siberian Sturgeon","authors":"German Benech-Correa,&nbsp;André Lasalle,&nbsp;Fréderic Brunet,&nbsp;Denise Vizziano-Cantonnet","doi":"10.1002/mrd.70074","DOIUrl":"10.1002/mrd.70074","url":null,"abstract":"<div>\u0000 \u0000 <p>The search for male-biased genes that may drive sexual differentiation in sturgeon gonadal tissue has remained largely fruitless until now. This study explored the presence, expression, and sexual steroid regulation of gonadal soma-derived factor (Gsdf) in Siberian sturgeon (<i>Acipenser baerii</i>). Three sequences compatible with the protein-coding gene <i>gsdf</i> were identified in a Siberian sturgeon gonadal transcriptome database. The phylogenetic analysis shows the presence of two paralogues <i>gsdfa</i> and <i>gsdfb</i>, and two clearly identifiable <i>gsdfa</i> isoforms (<i>gsdfa1</i> and <i>gsdfa2</i>). <i>gsdfa</i> was expressed in sex-undifferentiated gonads, brain, hypophysis, heart, interrenal, intestine, liver, stomach, embryos and larvae, while <i>gsdfb</i> was specific to the gonads. <i>gsdfb</i> showed significantly higher expression in genetically sexed males during late stage of sex differentiation. In contrast, <i>gsdfa1</i> and <i>gsdfa2</i> showed similar gonadal expression levels in both sexes. In terms of sensitivity to sexual steroids, only <i>gsdfb</i> was repressed significantly and in a dose-dependent manner by estrogens. Taken together, these results indicate both that <i>gsdfb</i> is part of the male gonadal differentiation pathway in sturgeon and that its repression by estrogens is fundamental for female development.</p>\u0000 </div>","PeriodicalId":18856,"journal":{"name":"Molecular Reproduction and Development","volume":"92 12","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145708100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}