Molecular Imaging最新文献

Effect of the TAAR1 Partial Agonist Ralmitaront on Presynaptic Dopamine Synthesis Capacity Measured Using [¹⁸F]DOPA PET in Naïve and Cocaine-Treated Mice. 使用[18F]DOPA PET测量TAAR1部分激动剂Ralmitaront对新生小鼠和可卡因处理小鼠突触前多巴胺合成能力的影响

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241299546

David R Bonsall, Michelle Kokkinou, Els F Halff, Grazia Rutigliano, Sac-Pham Tang, Mattia Veronese, Elaine E Irvine, Dominic J Withers, Lisa A Wells, Sridhar Natesan, Irene Gerlach, Štefan Holiga, Marius C Hoener, Oliver D Howes

{"title":"Effect of the TAAR1 Partial Agonist Ralmitaront on Presynaptic Dopamine Synthesis Capacity Measured Using [18F]DOPA PET in Naïve and Cocaine-Treated Mice.","authors":"David R Bonsall, Michelle Kokkinou, Els F Halff, Grazia Rutigliano, Sac-Pham Tang, Mattia Veronese, Elaine E Irvine, Dominic J Withers, Lisa A Wells, Sridhar Natesan, Irene Gerlach, Štefan Holiga, Marius C Hoener, Oliver D Howes","doi":"10.1177/15353508241299546","DOIUrl":"10.1177/15353508241299546","url":null,"abstract":"Purpose: Elevated dopamine synthesis capacity is part of the pathophysiology of schizophrenia thought to underlie psychosis. Drugs that reduce this phenomenon could thus be potential treatments for these disorders. In this study, we evaluated the ability of the trace amine-associated receptor 1 (TAAR1) partial agonist ralmitaront to reduce presynaptic dopamine synthesis capacity.Procedures: Ralmitaront (3 mg/kg, i.p.), a TAAR1 partial agonist, was evaluated using [18F]DOPA PET for its ability to modulate presynaptic dopamine synthesis capacity in naïve mice as well as mice in an induced hyperdopaminergic state following acute cocaine administration (20 mg/kg, i.p.).Results: Cocaine treatment on its own did not induce elevated dopamine synthesis capacity when compared to the control group. Pretreatment with ralmitaront significantly reduced dopamine synthesis capacity when given either alone (44%) or in combination with the psychostimulant cocaine (50%) when compared to the control group.Conclusions: The TAAR1 agonist ralmitaront reduces striatal dopamine synthesis capacity, indexed as KiMod, both in naïve animals and when given prior to acute cocaine. This indicates the potential of TAAR1 agonism to address disorders characterized by striatal hyperdopaminergia.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241299546"},"PeriodicalIF":2.2,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homologous ¹¹¹In-Radiolabeled Platelet Survival and Sequestration Exploration for Refractory Immunologic Thrombocytopenic purpura in Children: A Strategy to Avoid Unnecessary Splenectomy. 儿童难治性免疫性血小板减少性紫癜的同源111in放射标记血小板生存和隔离探索：避免不必要的脾切除术的策略。

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-11-06 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241293961

Julien Dubois, Florentin Kucharczak, Léa De Neef, Virginie Kouyoumdjian, Gilles Palenzuela, Virginie Tunez, Denis Mariano-Goulart, Aurélie Bourdon, Tom Paunet

{"title":"Homologous 111In-Radiolabeled Platelet Survival and Sequestration Exploration for Refractory Immunologic Thrombocytopenic purpura in Children: A Strategy to Avoid Unnecessary Splenectomy.","authors":"Julien Dubois, Florentin Kucharczak, Léa De Neef, Virginie Kouyoumdjian, Gilles Palenzuela, Virginie Tunez, Denis Mariano-Goulart, Aurélie Bourdon, Tom Paunet","doi":"10.1177/15353508241293961","DOIUrl":"10.1177/15353508241293961","url":null,"abstract":"Immunologic thrombocytopenic purpura (ITP) is a condition that affects four to 18 per 100 000 children every year. In most cases, spontaneous remission occurs, but splenectomy may be proposed. Exploring the site of platelet sequestration can help to better predict potential poor responders to splenectomy, but 111In-radiolabeled platelet scintigraphy (IPS) can be difficult to perform in children with very few platelets. A 12-year-old boy suffering from refractory ITP was referred for evaluation of platelet survival and sequestration and consideration of splenectomy. His platelet count consistently remained below 10 000/mm3. An exceptional procedure was set up to use homologous platelets to perform the IPS. Splenectomy was ruled out based on the results of 111In-radiolabeled homologous platelet scintigraphy. The attending pediatrician intensified medical treatments, resulting in a significant improvement in platelet count. This increase in platelet levels allowed for 111In-radiolabeled autologous platelet scintigraphy, which confirmed the absence of splenic sequestration. This allowed us to reject splenectomy in this child. Homologous IPS could help clinicians to choose splenectomy as a treatment option for ITP in children with a very low platelet count, and its use should be promoted after failed thrombopoietin receptor agonist (TPO-RA) treatment. More systematic studies are needed to confirm the predictive response to splenectomy of homologous IPS.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241293961"},"PeriodicalIF":2.2,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pharmacokinetic Model Determination of Time Activity Curves in Radiopharmaceutical Therapy. 放射性药物治疗中时间-活性曲线的药代动力学模型测定。

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-11-03 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241280015

Joseph Steiner, Brandon Nguyen, Farhad Jafari

{"title":"A Pharmacokinetic Model Determination of Time Activity Curves in Radiopharmaceutical Therapy.","authors":"Joseph Steiner, Brandon Nguyen, Farhad Jafari","doi":"10.1177/15353508241280015","DOIUrl":"10.1177/15353508241280015","url":null,"abstract":"Introduction and purpose: Radiopharmaceutical therapy (RPT) dosimetry can be challenging to perform due to sparse data measurements and variations in how the time activity curve (TAC) is determined. In this work, a single system of equations was theoretically derived to estimate the TAC.Methods: A pharmacokinetic (PK) model was developed to estimate patient specific rate constants for a given set of body compartments. The PK model and an optimizer were numerically implemented to determine the rate constants and, using these physiologic data, to generate TACs and time integrated activities (TIAs) for 3 tissue systems from clinical data gathered in 5 patients. A fourth (aggregate) tissue compartment is added using conservation of activity considerations.Results: Feasibility of the PK model was demonstrated by successfully generating TACs and TIAs for all patients in a manner comparable to existing methods in the literature. The data are compared to smaller sampling regimes. Differences between the 3- and 4-compartment models show that conservation of activity considerations should be part of TAC estimations.Conclusion: The results here suggest a new paradigm in RPT in using the rate constants so identified as a diagnostic tool and as a vehicle to achieving individualized tumorcidal dose and/or the maximum tolerable dose to normal tissues.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241280015"},"PeriodicalIF":2.2,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Situ Mapping of the Glucose Metabolism Heterogeneity in Atherosclerosis: Correlation With 2-Deoxyglucose Uptake. 动脉粥样硬化中葡萄糖代谢异质性的原位图：与 2-脱氧葡萄糖摄取的相关性。

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-09-17 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241280573

Joseph Haddad, Selim Demirdelen, Clayton E Barnes, Steven A Leers, Sina Tavakoli

{"title":"In Situ Mapping of the Glucose Metabolism Heterogeneity in Atherosclerosis: Correlation With 2-Deoxyglucose Uptake.","authors":"Joseph Haddad, Selim Demirdelen, Clayton E Barnes, Steven A Leers, Sina Tavakoli","doi":"10.1177/15353508241280573","DOIUrl":"10.1177/15353508241280573","url":null,"abstract":"Objective: 2-Deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) is widely used for noninvasive imaging of atherosclerosis. However, knowledge about metabolic processes underlying [18F]FDG uptake is mostly derived from in vitro cell culture studies, which cannot recapitulate the complexities of the plaque microenvironment. Here, we sought to address this gap by in situ mapping of the activity of selected major dehydrogenases involved in glucose metabolism in atherosclerotic plaques.Methods: In situ activity of lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PD), succinate dehydrogenase (SDH), and isocitrate dehydrogenase (IDH) was assessed in plaques from murine aortic root and brachiocephalic arteries and human carotid arteries. High-resolution 2-deoxy-D-[1,2-3H]glucose ([3H]2-deoxyglucose) autoradiography of murine brachiocephalic plaques was performed.Results: LDH activity was heterogeneous throughout the plaques with the highest activity in medial smooth muscle cells (SMCs). G6PD activity was mostly confined to the medial layer and to a lesser extent to SMCs along the fibrous cap. SDH and IDH activities were minimal in plaques. Plaque regions with increased [3H]2-deoxyglucose uptake were associated with a modestly higher LDH, but not G6PD, activity.Conclusions: Our study reveals a novel aspect of the metabolic heterogeneity of the atherosclerotic plaques, enhancing our understanding of the complex immunometabolic biology that underlies [18F]FDG uptake in atherosclerosis.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241280573"},"PeriodicalIF":2.2,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11577107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Tumor Non-specific and PD-L1 Specific Imaging by Near-Infrared Fluorescence/Cerenkov Luminescence Dual-Modality In-situ Imaging. 近红外荧光/切尔诺科夫荧光双模态原位成像对肿瘤非特异性和 PD-L1 特异性成像的比较

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-06-14 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241261473

Linhan Zhang, Lianmeng Zhao, Xue Lin, Sheng Zhao, Wenbin Pan, Dandan Wang, Zhongqi Sun, Jinping Li, Zonghui Liang, Rongjun Zhang, Huijie Jiang

{"title":"Comparison of Tumor Non-specific and PD-L1 Specific Imaging by Near-Infrared Fluorescence/Cerenkov Luminescence Dual-Modality In-situ Imaging.","authors":"Linhan Zhang, Lianmeng Zhao, Xue Lin, Sheng Zhao, Wenbin Pan, Dandan Wang, Zhongqi Sun, Jinping Li, Zonghui Liang, Rongjun Zhang, Huijie Jiang","doi":"10.1177/15353508241261473","DOIUrl":"10.1177/15353508241261473","url":null,"abstract":"Background: Labeled antibodies are excellent imaging agents in oncology to non-invasively visualize cancer-related antigens expression levels. However, tumor tracer uptake (TTU) of specific antibodies in-vivo may be inferior to non-specific IgG in some cases.Objectives: To explore factors affecting labeled antibody visualization by PD-L1 specific and non-specific imaging of nude mouse tumors.Methods: TTU was observed in RKO model on Cerenkov luminescence (CL) and near-infrared fluorescence (NIRF) imaging of radionuclide 131I or NIRF dyes labeled Atezolizumab and IgG. A mixture of NIRF dyes labeled Atezolizumab and 131I-labeled IgG was injected, and TTU was observed in the RKO and HCT8 model by NIRF/CL dual-modality in-situ imaging. TTU were observed by 131I-labeled Atezolizumab and IgG in-vitro distribution.Results: Labeled IgG concentrated more in tumors than Atezolizumab. NIRF/CL imaging in 24 to 168 h showed that TTU gradually decreased over time, which decreased more slowly on CL imaging compared to NIRF imaging. The distribution data in-vitro showed that TTU of 131I-labeled IgG was higher than that of 131I-labeled Atezolizumab at any time point.Conclusion: Non-specific IgG may not be suitable as a control for Atezolizumab in comparing tumor PD-L1 expression in nude mice via labeled antibody optical imaging under certain circumstances.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241261473"},"PeriodicalIF":2.2,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Relationship Between MRI Functional Imaging and Multiple Immunohistochemical Features of Glioma: A Noninvasive and More Precise Glioma Management. 胶质瘤核磁共振功能成像与多种免疫组化特征的关系研究：无创、更精确的胶质瘤管理。

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-06-11 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241261583

Jing Li, Jingtao Sun, Ning Wang, Yan Zhang

{"title":"Study on the Relationship Between MRI Functional Imaging and Multiple Immunohistochemical Features of Glioma: A Noninvasive and More Precise Glioma Management.","authors":"Jing Li, Jingtao Sun, Ning Wang, Yan Zhang","doi":"10.1177/15353508241261583","DOIUrl":"10.1177/15353508241261583","url":null,"abstract":"Objective: To investigate the performance of diffusion-tensor imaging (DTI) and hydrogen proton magnetic resonance spectroscopy (1H-MRS) parameters in predicting the immunohistochemistry (IHC) biomarkers of glioma.Methods: Patients with glioma confirmed by pathology from March 2015 to September 2019 were analyzed, the preoperative DTI and 1H-MRS images were collected, apparent diffusion coefficient (ADC) and fractional anisotropy (FA), in the lesion area were measured, the relative values relative ADC (rADC) and relative FA (rFA) were obtained by the ratio of them in the lesion area to the contralateral normal area. The peak of each metabolite in the lesion area of 1H-MRS image: N-acetylaspartate (NAA), choline (Cho), and creatine (Cr), and metabolite ratio: NAA/Cho, NAA/(Cho + Cr) were selected and calculated. The preoperative IHC data were collected including CD34, Ki-67, p53, S-100, syn, vimentin, NeuN, Nestin, and glial fibrillary acidic protein.Results: One predicting parameter of DTI was screened, the rADC of the Ki-67 positive group was lower than that of the negative group. Two parameters of 1H-MRS were found to have significant reference values for glioma grades, the NAA and Cr decreased as the grade of glioma increased, moreover, Ki-67 Li was negatively correlated with NAA and Cr.Conclusion: NAA and Cr have potential application value in predicting glioma grades and tumor proliferation activity. Only rADC has predictive value for Ki-67 expression among DTI parameters.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241261583"},"PeriodicalIF":2.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PET/CT in the Evaluation of CAR-T Cell Immunotherapy in Hematological Malignancies. PET/CT 在血液恶性肿瘤 CAR-T 细胞免疫疗法评估中的应用。

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-05-29 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241257924

Shashi B Singh, Sadikshya Bhandari, Shisir Siwakoti, Manoj Kumar, Rajshree Singh, Subarna Bhusal, Karuna Sharma, Samikshya Bhandari, Kishor Khanal

{"title":"PET/CT in the Evaluation of CAR-T Cell Immunotherapy in Hematological Malignancies.","authors":"Shashi B Singh, Sadikshya Bhandari, Shisir Siwakoti, Manoj Kumar, Rajshree Singh, Subarna Bhusal, Karuna Sharma, Samikshya Bhandari, Kishor Khanal","doi":"10.1177/15353508241257924","DOIUrl":"10.1177/15353508241257924","url":null,"abstract":"Chimeric antigen receptor (CAR)-T cell-based immunotherapy has emerged as a path-breaking strategy for certain hematological malignancies. Assessment of the response to CAR-T therapy using quantitative imaging techniques such as positron emission tomography/computed tomography (PET/CT) has been broadly investigated. However, the definitive role of PET/CT in CAR-T therapy remains to be established. [18F]FDG PET/CT has demonstrated high sensitivity and specificity for differentiating patients with a partial and complete response after CAR-T therapy in lymphoma. The early therapeutic response and immune-related adverse effects such as cytokine release syndrome and immune effector cell-associated neurotoxicity syndrome can also be detected on [18F]FDG PET images. In otherwise asymptomatic lymphoma patients with partial response following CAR-T therapy, the only positive findings could be abnormal PET/CT results. In multiple myeloma, a negative [18F]FDG PET/CT after receiving B-cell maturation antigen-directed CAR-T therapy has been associated with a favorable prognosis. In leukemia, [18F]FDG PET/CT can detect extramedullary metastases and treatment responses after therapy. Hence, PET/CT is a valuable imaging tool for patients undergoing CAR-T therapy for pretreatment evaluation, monitoring treatment response, assessing safety, and guiding therapeutic strategies. Developing guidelines with standardized cutoff values for various PET parameters and tumor cell-specific tracers may improve the efficacy and safety of CAR-T therapy.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"23 ","pages":"15353508241257924"},"PeriodicalIF":2.2,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11208886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining Nuclear Medicine With Other Modalities: Future Prospect for Multimodality Imaging. 核医学与其他模式的结合：多模式成像的未来展望。

IF 2.2 4区医学

Molecular Imaging Pub Date : 2024-04-30 eCollection Date: 2024-01-01 DOI: 10.1177/15353508241245265

Stephen J Archibald, Jason P Holland, Aruna Korde, Andre F Martins, Adam J Shuhendler, Peter J H Scott

引用次数: 0

Optical and MRI Multimodal Tracing of Stem Cells In Vivo 体内干细胞的光学和磁共振成像多模态追踪

IF 2.8 4区医学

Molecular Imaging Pub Date : 2023-12-19 DOI: 10.1155/2023/4223485

Jia Yang, Min Yan, Zhong Wang, Cong Zhang, Miao Guan, Zhenglong Sun

{"title":"Optical and MRI Multimodal Tracing of Stem Cells In Vivo","authors":"Jia Yang, Min Yan, Zhong Wang, Cong Zhang, Miao Guan, Zhenglong Sun","doi":"10.1155/2023/4223485","DOIUrl":"https://doi.org/10.1155/2023/4223485","url":null,"abstract":"Stem cell therapy has shown great clinical potential in oncology, injury, inflammation, and cardiovascular disease. However, due to the technical limitations of the in vivo visualization of transplanted stem cells, the therapeutic mechanisms and biosafety of stem cells in vivo are poorly defined, which limits the speed of clinical translation. The commonly used methods for the in vivo tracing of stem cells currently include optical imaging, magnetic resonance imaging (MRI), and nuclear medicine imaging. However, nuclear medicine imaging involves radioactive materials, MRI has low resolution at the cellular level, and optical imaging has poor tissue penetration in vivo. It is difficult for a single imaging method to simultaneously achieve the high penetration, high resolution, and noninvasiveness needed for in vivo imaging. However, multimodal imaging combines the advantages of different imaging modalities to determine the fate of stem cells in vivo in a multidimensional way. This review provides an overview of various multimodal imaging technologies and labeling methods commonly used for tracing stem cells, including optical imaging, MRI, and the combination of the two, while explaining the principles involved, comparing the advantages and disadvantages of different combination schemes, and discussing the challenges and prospects of human stem cell tracking techniques.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"76 1","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138744282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green 促性腺激素释放激素类似物修饰的吲哚菁绿在子宫内膜异位症的靶向成像和图像引导下的病变切除

IF 2.8 4区医学

Molecular Imaging Pub Date : 2023-12-04 DOI: 10.1155/2023/6674054

Jing Peng, Qiyu Liu, Tao Pu, Mingxing Zhang, Meng Zhang, Ming Du, Guiling Li, Xiaoyan Zhang, Congjian Xu

{"title":"Targeted Imaging of Endometriosis and Image-Guided Resection of Lesions Using Gonadotropin-Releasing Hormone Analogue-Modified Indocyanine Green","authors":"Jing Peng, Qiyu Liu, Tao Pu, Mingxing Zhang, Meng Zhang, Ming Du, Guiling Li, Xiaoyan Zhang, Congjian Xu","doi":"10.1155/2023/6674054","DOIUrl":"https://doi.org/10.1155/2023/6674054","url":null,"abstract":"Objective. In this study, we utilized gonadotropin-releasing hormone analogue-modified indocyanine green (GnRHa-ICG) to improve the accuracy of intraoperative recognition and resection of endometriotic lesions. Methods. Gonadotropin-releasing hormone receptor (GnRHR) expression was detected in endometriosis tissues and cell lines via immunohistochemistry and western blotting. The in vitro binding capacities of GnRHa, GnRHa-ICG, and ICG were determined using fluorescence microscopy and flow cytometry. In vivo imaging was performed in mouse models of endometriosis using a near-infrared fluorescence (NIRF) imaging system and fluorescence navigation system. The ex vivo binding capacity was determined using confocal fluorescence microscopy. Results. GnRHa-ICG exhibited a significantly stronger binding capacity to endometriotic cells and tissues than ICG. In mice with endometriosis, GnRHa-ICG specifically imaged endometriotic tissues (EMTs) after intraperitoneal administration, whereas ICG exhibited signals in the intestine. GnRHa-ICG showed the highest fluorescence signals in the EMTs at 2 h and a good signal-to-noise ratio at 48 h postadministration. Compared with traditional surgery under white light, targeted NIRF imaging-guided surgery completely resected endometriotic lesions with a sensitivity of 97.3% and specificity of 77.8%. No obvious toxicity was observed in routine blood tests, serum biochemicals, or histopathology in mice. Conclusions. GnRHa-ICG specifically recognized and localized endometriotic lesions and guided complete resection of lesions with high accuracy.","PeriodicalId":18855,"journal":{"name":"Molecular Imaging","volume":"507 ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0