Mycology最新文献_第2页

Functionally redundant Rho GTPases Cdc42 and RacA regulate aflatoxin synthesis and pathogenicity in Aspergillus flavus by controlling morphogenesis, oxidative balance and energy metabolism. 功能冗余的Rho gtpase Cdc42和RacA通过控制黄曲霉的形态发生、氧化平衡和能量代谢来调节黄曲霉毒素的合成和致病性。

IF 4.4 2区生物学

Mycology Pub Date : 2025-07-10 eCollection Date: 2026-01-01 DOI: 10.1080/21501203.2025.2527381

Jia Xu, Junhe Ren, Yanyan Zhang, Zhuoyu Han, Qing Kong

{"title":"Functionally redundant Rho GTPases Cdc42 and RacA regulate aflatoxin synthesis and pathogenicity in Aspergillus flavus by controlling morphogenesis, oxidative balance and energy metabolism.","authors":"Jia Xu, Junhe Ren, Yanyan Zhang, Zhuoyu Han, Qing Kong","doi":"10.1080/21501203.2025.2527381","DOIUrl":"10.1080/21501203.2025.2527381","url":null,"abstract":"Rho GTPases Cdc42 and RacA exhibit significant sequence homology and are conserved across eukaryotic species. These proteins function as molecular switches within various signal transduction pathways by cycling between GTP-bound (active) and GDP-bound (inactive) states. However, their specific functions in Aspergillus flavus remain largely unexplored. In this study, CRISPR/Cas9 system utilizing 5S rRNA and tRNA-gRNA tandem arrays was developed, achieving over 95% single-gene and 75% double-gene editing efficiencies. Phenotypic and transcriptome analyses revealed significant functional redundancy between cdc42 and racA. In the mutants, conidia germination was markedly accelerated, with early germination driven by increased hydrolase activity and ATP levels. The loss of either cdc42 or racA resulted in reduced pathogenicity, compromised cell wall integrity, diminished aflatoxin production, and disrupted oxidative systems. These proteins regulate the generation of ROS through their interaction with NoxR, the regulatory subunit of NADPH oxidase (Nox). Cdc42 and RacA exhibited opposing roles in fatty acid β-oxidation and pyruvate metabolism. The simultaneous loss of function in both genes is lethal, as evidenced by the inability of the ∆cdc42racA tetOn mutant to grow on plates lacking doxycycline. The study reveals the critical roles of the closely related genes cdc42 and racA in the growth, development, and metabolism of A. flavus. These findings identify potential targets for mitigating the harmful effects of A. flavus and aflatoxins.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"17 1","pages":"2527381"},"PeriodicalIF":4.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MtfA, a C2H2 transcriptional regulator, negatively regulates PRPS2-mediated biosynthesis of the adenosine analogue acadesine in Fusarium solani. MtfA是一种C2H2转录调节因子，负向调节prps2介导的茄枯菌腺苷类似物乙酰腺苷的生物合成。

IF 4.4 2区生物学

Mycology Pub Date : 2025-06-19 eCollection Date: 2026-01-01 DOI: 10.1080/21501203.2025.2514146

Qirong Chen, Jiankang Wang, Rongfei Liu, Hui Li, Zhangjiang He, Jichuan Kang

{"title":"MtfA, a C2H2 transcriptional regulator, negatively regulates PRPS2-mediated biosynthesis of the adenosine analogue acadesine in Fusarium solani.","authors":"Qirong Chen, Jiankang Wang, Rongfei Liu, Hui Li, Zhangjiang He, Jichuan Kang","doi":"10.1080/21501203.2025.2514146","DOIUrl":"10.1080/21501203.2025.2514146","url":null,"abstract":"Endogenous AICAR (acadesine) demonstrates significant therapeutic potential as a phase III clinical agent for the treatment of adverse cardiovascular reactions to coronary artery bypass grafting and as a phase I/II clinical agent for chronic lymphocytic leukaemia. However, its biosynthetic mechanism remains poorly defined. Our previous study demonstrated that AICAR was significantly enriched in the Fusarium solani mutant veA OE14, which overexpressed the global regulator VeA. We found that phosphoribosyl pyrophosphate synthase 2, viz., PRPS2 (PrsA) is regulated by VeA and involved in AICAR synthesis. Deletion of PRPS2 resulted in a significant 17% (μg/mg) reduction in AICAR production in the strain (p < 0.05), whereas its overexpression led to a statistically significant 1.2-fold increase in AICAR content (p < 0.01). Furthermore, we characterised the transcription factor MtfA, which inhibits the expression of PRPS2 gene and is negatively regulated by VeA. Further validation by yeast one-hybrid assay showed that MtfA directly regulated the PRPS2 gene, while AICAR was significantly increased in the mtfA deletion mutant. The above results indicate that the global regulator VeA negatively regulates the transcription factor MtfA, which in turn targets negatively regulating transcriptional levels of PRPS2 to mediate AICAR biosynthesis in F. solani.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"17 1","pages":"2514146"},"PeriodicalIF":4.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minutellins E-I and daldinone L, new secondary metabolites from different species of the Hypoxylaceae (Xylariales, Ascomycota). 木耳科子囊菌科不同种的新次生代谢产物Minutellins E-I和daldinone L。

IF 4.4 2区生物学

Mycology Pub Date : 2025-06-18 eCollection Date: 2026-01-01 DOI: 10.1080/21501203.2025.2512962

Christopher Lambert, Mohammad Javad Pourmoghaddam, Esteban Charria-Girón, Frank Surup, Seyed Akbar Khodaparast, Hermann Voglmayr, Irmgard Krisai-Greilhuber, Marc Stadler

{"title":"Minutellins E-I and daldinone L, new secondary metabolites from different species of the Hypoxylaceae (Xylariales, Ascomycota).","authors":"Christopher Lambert, Mohammad Javad Pourmoghaddam, Esteban Charria-Girón, Frank Surup, Seyed Akbar Khodaparast, Hermann Voglmayr, Irmgard Krisai-Greilhuber, Marc Stadler","doi":"10.1080/21501203.2025.2512962","DOIUrl":"10.1080/21501203.2025.2512962","url":null,"abstract":"Fungi of the family Hypoxylaceae (Xylariales, Ascomycota) are ubiquitously distributed and fulfil important ecological roles as saprobes, pathogens, and endophytes. Members of this family tend to store large amounts of secondary metabolites in their carbonaceous stromatal tissue commonly formed on colonised wood. This feature is of both taxonomic and chemical value by serving as chemotaxonomic markers and sources of potential new and bioactive compounds. Despite tremendous progress in the characterisation of Hypoxylaceae both in terms of genomics and secondary metabolomics, many unknown metabolites remain to be identified or elucidated. Here, we report on the polyphasic, i.e. morphological, chemical, and genetical analysis of Hypoxylon and Annulohypoxylon spp. collected from the Iranian shore of the Caspian Sea and a chemotaxonomic study of the constituents of a concurrently studied specimen of A. michelianum. A new daldinone derivative and five new minutellin-type azaphilones from stromatal tissues of A. substygium and H. lateripigmentum, respectively, were identified in the course of this study by high-performance liquid chromatography/mass spectrometry coupling (HPLC-MS) and structurally elucidated by nuclear magnetic resonance (NMR) spectroscopy. We realised that the structure of daldinone A was misassigned and in fact equals daldinone C. Finally yet importantly, the distribution of minutellin-type azaphilones throughout the Hypoxylaceae is discussed.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"17 1","pages":"2512962"},"PeriodicalIF":4.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007406/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxymatrine ameliorates Malassezia overgrowth-induced psoriasis in vivo and in vitro by inhibiting the biofilm formation and inflammation. 氧化苦参碱通过抑制生物膜的形成和炎症来改善马拉色菌过度生长诱导的银屑病。

IF 4.4 2区生物学

Mycology Pub Date : 2025-06-10 eCollection Date: 2026-01-01 DOI: 10.1080/21501203.2025.2511903

Miao-Miao Liu, Jie Bai, Zi-Ye Tian, Ting-Ting Zheng, Teun Boekhout, Qi-Ming Wang

{"title":"Oxymatrine ameliorates Malassezia overgrowth-induced psoriasis in vivo and in vitro by inhibiting the biofilm formation and inflammation.","authors":"Miao-Miao Liu, Jie Bai, Zi-Ye Tian, Ting-Ting Zheng, Teun Boekhout, Qi-Ming Wang","doi":"10.1080/21501203.2025.2511903","DOIUrl":"10.1080/21501203.2025.2511903","url":null,"abstract":"The basidiomycetous yeast genus Malassezia is involved in the exacerbation of psoriatic lesions. Oxymatrine (OMT), a quinoline alkaloid derived from Sophora flavescens, exhibits diverse pharmacological properties, including anti-inflammatory, anticancer, and antiviral effects. However, whether OMT exerts therapeutic effects against Malassezia-associated psoriasis remains unclear. This work aimed to study the antifungal and antibiofilm effect of OMT on several Malassezia species and the therapeutic benefits of OMT on Malassezia-associated psoriasis in vivo and in vitro. Treatment with 0.64 mg/mL OMT showed decreasing levels of biofilm formation of Malassezia species. Histomorphology and functional analyses demonstrated that OMT treatment effectively alleviated Malassezia-induced psoriatic lesions and repaired skin barrier integrity. Furthermore, the results demonstrate that OMT significantly reduced the levels of malonaldehyde, interleukin (IL)-6, IL-17, IL-23, and tumour necrosis factor (TNF)-α while promoting the activation of superoxide dismutase, catalase, and glutathione. OMT also reversed Malassezia-associated apoptosis and decreased the expression of the STAT3/Nf-κB/p-Nf-κB signalling pathway. Additionally, OMT reduces the nuclear expression of AhR/Nrf2 in Malassezia-stimulated HaCaT cells. In summary, this study demonstrated that OMT inhibits Malassezia biofilm formation and ameliorates Malassezia-associated psoriasis by modulating oxidative stress, inflammation, and apoptosis via STAT3/Nf-κB and AhR/Nrf2 pathways.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"17 1","pages":"2511903"},"PeriodicalIF":4.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox engineering of thermophilic fungus Myceliophthora thermophila enhances production of L‑malic acid by consolidated bioprocessing. 嗜热真菌嗜热霉菌的氧化还原工程通过强化生物处理提高L -苹果酸的产量。

IF 4.4 2区生物学

Mycology Pub Date : 2025-06-10 eCollection Date: 2026-01-01 DOI: 10.1080/21501203.2025.2509017

Yuying Zhang, Rui Fan, Taju Wu, Shuying Gu, Defei Liu, Liangcai Lin, Min Wang, Jingen Li, Chaoguang Tian

{"title":"Redox engineering of thermophilic fungus Myceliophthora thermophila enhances production of L‑malic acid by consolidated bioprocessing.","authors":"Yuying Zhang, Rui Fan, Taju Wu, Shuying Gu, Defei Liu, Liangcai Lin, Min Wang, Jingen Li, Chaoguang Tian","doi":"10.1080/21501203.2025.2509017","DOIUrl":"10.1080/21501203.2025.2509017","url":null,"abstract":"Consolidated bioprocessing (CBP) is a promising strategy for low-cost malic acid production from plant biomass. In this study, we report a comprehensive redox engineering strategy to redirect reducing equivalents towards the reductive tricarboxylic acid (rTCA) pathway for enhanced malic acid production directly from cellulose in the thermophilic fungus Myceliophthora thermophila JG207. Firstly, deletion of key NADH-consuming pathways, including glycerol-3-phosphate dehydrogenase (gpdh) and mitochondrial external NADH dehydrogenases (nde1 and nde2), resulted in a significant increase in the NADH/NAD+ ratio and a corresponding 17% increase in malic acid titre. Subsequent removal of alternative oxidase (AOX) further heightened the NADH pool, although it induced oxidative stress and slowed growth. To alleviate redox imbalance, we overexpressed the native transhydrogenase SthA, which effectively converted NADPH to NADH and improved malate production by an additional 6%. Fine-tuning NAD+ kinase (UTR1) expression further optimised cofactor homoeostasis, bringing about the highest malate titre of 89.2 g/L, a 38.9% improvement over the starting strain JG207. Finally, overexpression of lytic polysaccharide monooxygenase gene (lpmo) and cellobiose dehydrogenase gene (cdh) enhanced cellulose degradation, shortening the fermentation cycle by two days on Avicel and crushed corncob. This work not only advances the fundamental understanding of redox metabolism in filamentous fungi but also provides a proof-of-concept for sustainable organic acid production from inexpensive lignocellulosic feedstocks.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"17 1","pages":"2509017"},"PeriodicalIF":4.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic contributions to Pleosporales and Kirschsteiniotheliales from the Xizang Autonomous Region, China. 文章标题西藏省多孢门和姬孢门的分类学贡献。

IF 4.4 2区生物学

Mycology Pub Date : 2025-05-27 eCollection Date: 2026-01-01 DOI: 10.1080/21501203.2025.2493072

Shu-Cheng He, Kevin D Hyde, Ruvishika S Jayawardena, Vinodhini Thiyagaraja, Dhanushka N Wanasinghe, Yun-Wei Zhao, Zhi-Yang Wang, Tong Cai, Yan-Yan Yang, F Al-Otibi, Zhuliang Yang, Qi Zhao

{"title":"Taxonomic contributions to Pleosporales and Kirschsteiniotheliales from the Xizang Autonomous Region, China.","authors":"Shu-Cheng He, Kevin D Hyde, Ruvishika S Jayawardena, Vinodhini Thiyagaraja, Dhanushka N Wanasinghe, Yun-Wei Zhao, Zhi-Yang Wang, Tong Cai, Yan-Yan Yang, F Al-Otibi, Zhuliang Yang, Qi Zhao","doi":"10.1080/21501203.2025.2493072","DOIUrl":"10.1080/21501203.2025.2493072","url":null,"abstract":"The Xizang Autonomous Region, China, is a key ecological security barrier, renowned for its rich biodiversity and unique resources. It supports diverse life forms, including fungi, which are vital for ecological balance and the restoration of the region's ecosystems. This study presents the results of a microfungal survey conducted on the Plateau, focusing on the orders Pleosporales and Kirschsteiniotheliales within the class Dothideomycetes. Based on morphological characteristics and multi-gene (ITS, LSU, SSU, tef1, tub2, and rpb2) phylogeny analyses, we report the discovery of 10 new taxa, including one new genus Neotriplosphaeria and nine new species, Kirschsteiniothelia linzhiensis, K. yadongensis, Neotriplosphaeria yadongensis, Periconia linzhiensis, Tetraploa linzhiensis, Torula dingjieensis, T. yadongensis, Trichobotrys motuoensis, Triseptatospora yadongensis, and one new host record Periconia spodiopogonis. The findings provide valuable insights into the morphological and phylogenetic relationships of these fungi, highlighting the Plateau's significant role in fungal diversity. This study enriches our understanding of fungal biodiversity in extreme environments and underscores the importance of continued exploration in this region.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"17 1","pages":"2493072"},"PeriodicalIF":4.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13007439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trichoderma species from typical forests system in China: Geographical distribution and comparative genome-wide evaluation of selected species related to biocontrol. 中国典型森林系统木霉物种：与生物防治相关的选择物种的地理分布和比较全基因组评价。

IF 4.4 2区生物学

Mycology Pub Date : 2025-05-26 eCollection Date: 2025-01-01 DOI: 10.1080/21501203.2025.2500603

Kai Dou, Zhenxu Bai, Fuli Zhang, Hongyi Liu, Xifen Zhang, Cheng Zhang, Yaqian Li, Jie Chen

{"title":"Trichoderma species from typical forests system in China: Geographical distribution and comparative genome-wide evaluation of selected species related to biocontrol.","authors":"Kai Dou, Zhenxu Bai, Fuli Zhang, Hongyi Liu, Xifen Zhang, Cheng Zhang, Yaqian Li, Jie Chen","doi":"10.1080/21501203.2025.2500603","DOIUrl":"10.1080/21501203.2025.2500603","url":null,"abstract":"In this study, we isolated 633 Trichoderma strains from 15 forest types across six climate zones. Our findings revealed that species diversity was highly underestimated, with 239 isolates identified as new species based on ITS, rpb2, and tef1 sequences. In tropical and subtropical regions, the mean values for the haplotype Chao1 richness index and the Shannon-Weiner diversity index are 73 and 3, respectively, significantly higher than those in temperate regions. Trichoderma cf. taiwanense was the most widely distributed, followed by T. asperellum, rather than widely reported T. harzianum. The maximum entropy model was used to analyse the effects of climatic factors on distribution of T. cf. taiwanense and T. asperellum, and showed that precipitation in the warmest quarter was the most important factor influencing their distribution. T. cf. taiwanense zj625y demonstrated significantly higher antagonistic ability, outperforming the other tested Trichoderma species. The genomic characteristics of T. cf. taiwanense were firstly revealed with 36.49 Mbp in size and contained 17 genes of β-1,3-glucanase and 24 genes of chitinase genes. Additionally, the activities of endochitinase, endo-1,3(4)-β-glucanase, and metallopeptidase were highly expressed in culture. Metabolomic analysis further identified specific substances produced by T. cf. taiwanense zj625y, such as ergothioneine, (3-carboxypropyl) trimethylammonium cation, NG-dimethyl-L-arginine (ADMA), N-acetyl-D-glucosamine, stearic acid, tyramine, citrate, L-leucine, and acetylcarnitine, which contribute to its potential antagonism and environmental adaptability.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"16 4","pages":"1690-1710"},"PeriodicalIF":4.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional diversification of epidithiodiketopiperazine methylation and oxidation towards pathogenic fungi. 附硫碘二酮哌嗪甲基化和氧化对病原菌的功能多样化。

IF 4.4 2区生物学

Mycology Pub Date : 2025-05-21 eCollection Date: 2025-01-01 DOI: 10.1080/21501203.2025.2496190

Shengquan Zhang, Peng-Lin Wei, Yuanyuan Li, Zedong Ren, Jie Fan, Wen-Bing Yin

{"title":"Functional diversification of epidithiodiketopiperazine methylation and oxidation towards pathogenic fungi.","authors":"Shengquan Zhang, Peng-Lin Wei, Yuanyuan Li, Zedong Ren, Jie Fan, Wen-Bing Yin","doi":"10.1080/21501203.2025.2496190","DOIUrl":"10.1080/21501203.2025.2496190","url":null,"abstract":"The genus Trichoderma plays a vital role in agriculture by promoting plant growth, enhancing nutrient uptake, and protecting crops from pathogens through biocontrol mechanisms. This can be largely attributed to its production of diverse secondary metabolites (SMs), including epidithiodiketopiperazines (ETPs). Our previous study has reported the complex biosynthesis of α, β'-disulfide bridged ETPs, in which TdaH and TdaG are highly conserved in catalyzing C6'-O-methylation and C4, C5-epoxidation, respectively. Here we proved the functional diversification of ETP methylation and oxidation by TdaH and TdaG towards eleven pathogenic fungi, including Fusarium, Aspergillus, and Botrytis species. Elimination of C6'-O-methylation and C4, C5-epoxidation reduced the antagonistic effects of Trichoderma hypoxylon against various pathogenic fungi. However, each deletion mutant showed varying antagonistic effects against different pathogenic fungi. Our results highlight the importance of ETP structural diversity in T. hypoxylon's ecological adaptation and biocontrol potential, offering insights into developing enhanced antifungal agents against plant pathogens.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"16 3","pages":"1418-1431"},"PeriodicalIF":4.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12422046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vacuolar protein sorting-associated protein Vps17 regulates sexual reproduction and virulence of Cryptococcus neoformans. 液泡蛋白分选相关蛋白Vps17调控新生隐球菌有性繁殖和毒力。

IF 4.4 2区生物学

Mycology Pub Date : 2025-05-16 eCollection Date: 2025-01-01 DOI: 10.1080/21501203.2025.2499538

Meng-Ru Guo, Jie-Shu Guo, Jun-Ming Chen, Tong-Bao Liu

{"title":"Vacuolar protein sorting-associated protein Vps17 regulates sexual reproduction and virulence of Cryptococcus neoformans.","authors":"Meng-Ru Guo, Jie-Shu Guo, Jun-Ming Chen, Tong-Bao Liu","doi":"10.1080/21501203.2025.2499538","DOIUrl":"10.1080/21501203.2025.2499538","url":null,"abstract":"Cryptococcus neoformans, an opportunistic fungal pathogen, poses significant health risks by causing severe meningitis in both immunocompromised and immunocompetent individuals. In this study, we identified the vacuolar protein sorting-associated protein Vps17, a crucial Retromer complex component, in C. neoformans. Sequence analysis revealed that Cryptococcus Vps17 contains a PX and a BAR domain, classifying it within a conserved family of SNX proteins interacting with inositol phosphate. Expression pattern analysis indicated that the VPS17 gene is active throughout all developmental stages of C. neoformans, with Vps17 localised on vesicles and unaffected by external stressors. Deletion of the VPS17 gene resulted in reduced fungal capsule size and increased sensitivity to various stressors like SDS, NaCl, and KCl, highlighting its role in maintaining cell membrane integrity. Significantly, the absence of Vps17 hindered sexual reproduction, preventing basidiospore formation and meiosis following nuclear fusion. In a murine systemic infection model, the virulence of the vps17Δ mutant was significantly reduced. Our findings suggest that Vps17 plays a vital role in regulating sexual reproduction and pathogenicity in C. neoformans by influencing the expression of numerous related genes.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"16 4","pages":"1711-1733"},"PeriodicalIF":4.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New antifungal chlorinated orsellinic aldehydes from the deep-sea-derived fungus Acremonium sclerotigenum LW14. 从深海源真菌Acremonium sclerotigenum LW14中提取新的抗真菌氯代orsellinic醛。

IF 4.4 2区生物学

Mycology Pub Date : 2025-04-11 eCollection Date: 2025-01-01 DOI: 10.1080/21501203.2025.2485477

Ruiyun Huo, Yu Tu, Chang Liu, Guangrong Zi, Ying Shi, Jinwei Ren, Lei Cai, Ling Liu

{"title":"New antifungal chlorinated orsellinic aldehydes from the deep-sea-derived fungus Acremonium sclerotigenum LW14.","authors":"Ruiyun Huo, Yu Tu, Chang Liu, Guangrong Zi, Ying Shi, Jinwei Ren, Lei Cai, Ling Liu","doi":"10.1080/21501203.2025.2485477","DOIUrl":"10.1080/21501203.2025.2485477","url":null,"abstract":"Two new pairs of chlorinated orsellinic aldehyde enantiomers acresorcinols A and B (1a/1b and 2a/2b), and three orsellinic aldehydes, acresorcinols C-E (3-5) were discovered from the extract of the deep-sea-derived fungus Acremonium sclerotigenum LW14. Their structures, including absolute configurations, were experimentally elucidated by spectroscopic analysis, NMR calculations with DP4+ probability analysis, and ECD calculations. Compounds 1 and 2 exemplify the first reported chlorinated orsellinic aldehydes, characterised by a distinctive 6/5-5 tricyclic core structure with a bridged framework. Acresorcinol C (3) was a rare carbon-bridged resorcinol dimer via a methylene bridge. The bioassay results showed that all compounds exhibited antifungal activities against Cryptococcus gattii 3271G1 at 32 μg/mL. Compounds 1-3 showed antifungal effects against C. gattii 3271G1, displaying MIC values of 8, 16, and 16 µg/mL, respectively.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"16 4","pages":"1797-1806"},"PeriodicalIF":4.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12667284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145660961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0