Molecular medicine reports最新文献

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Cholesterol metabolism in LUAD progression: GJB3 as a key target for cell‑based therapeutic interventions. LUAD进展中的胆固醇代谢:GJB3是基于细胞的治疗干预的关键靶点。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-09-26 DOI: 10.3892/mmr.2025.13694
Qihang Yan, Wuguang Chang, Wingshing Wong, Li Gong, Dachuan Liang, Jie Yang, Junye Wang
{"title":"Cholesterol metabolism in LUAD progression: GJB3 as a key target for cell‑based therapeutic interventions.","authors":"Qihang Yan, Wuguang Chang, Wingshing Wong, Li Gong, Dachuan Liang, Jie Yang, Junye Wang","doi":"10.3892/mmr.2025.13694","DOIUrl":"10.3892/mmr.2025.13694","url":null,"abstract":"<p><p>Cholesterol metabolism reprogramming serves a pivotal role in tumor onset and progression. The present study investigated lung adenocarcinoma (LUAD), focusing on the regulatory impact of cholesterol metabolism‑related genes (CMRGs). Consensus clustering identified distinct cholesterol metabolism‑related clusters in LUAD, followed by survival analysis and immune infiltration profiling for each cluster. A predictive model, constructed using cluster‑specific differentially expressed genes and the LASSO algorithm, was validated with an independent dataset. Furthermore, the model was utilized to predict potential responses to immunotherapy and chemotherapy for patients with LUAD. The functional role of the key gene GJB3 in LUAD progression was confirmed through <i>in vitro</i> experiments. Two distinct cholesterol metabolism‑related clusters were identified, exhibiting significant differences in prognosis, biological function and immune cell infiltration. A survival model, based on four genes, demonstrated strong predictive performance across multiple datasets. The low‑risk group showed improved responses to immunotherapy, while the high‑risk group exhibited heightened sensitivity to chemotherapy. <i>In vitro</i> assays revealed that GJB3 knockdown suppressed LUAD cell proliferation and invasion, significantly reducing the expression of epithelial‑mesenchymal transition‑related genes. These findings highlight CMRGs as potential prognostic biomarkers and suggest a foundation for personalized treatment strategies in LUAD.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Expression of Concern] Early anticoagulation therapy for severe burns complicated by inhalation injury in a rabbit model. [关注表达]兔严重烧伤合并吸入性损伤模型早期抗凝治疗
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-09-26 DOI: 10.3892/mmr.2025.13691
Zhong-Hua Fu, Guang-Hua Guo, Zhen-Fang Xiong, Xincheng Liao, Ming-Zhuo Liu, Jinhua Luo
{"title":"[Expression of Concern] Early anticoagulation therapy for severe burns complicated by inhalation injury in a rabbit model.","authors":"Zhong-Hua Fu, Guang-Hua Guo, Zhen-Fang Xiong, Xincheng Liao, Ming-Zhuo Liu, Jinhua Luo","doi":"10.3892/mmr.2025.13691","DOIUrl":"https://doi.org/10.3892/mmr.2025.13691","url":null,"abstract":"<p><p>Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that, regarding the histological images shown in Fig 1, the 'Sham' and the 'Model' data panels appeared to show areas of overlapping data with the 'Union' and the 'Antithrombin' panels respectively, albeit with very different zoom factors, such that data which were intended to show the results from differently performed experiments had apparently been derived from the same original sources. The authors were contacted by the Editorial Office to offer an explanation for these apparent anomalies in the presentation of the data in this paper; however, up to this time, no response from them has been forthcoming. Owing to the fact that the Editorial Office has been made aware of potential issues surrounding the scientific integrity of this paper, we are issuing an Expression of Concern to notify readers of this potential problem while the Editorial Office continues to investigate this matter further. [Molecular Medicine Reports 16: 7375‑7381, 2017; DOI: 10.3892/mmr.2017.7537].</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thrombolytic therapy efficacy in cardiopulmonary resuscitation: Investigating the effect of recombinant tissue plasminogen activator (Review). 溶栓治疗在心肺复苏中的疗效:重组组织型纤溶酶原激活剂作用的研究(综述)。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI: 10.3892/mmr.2025.13700
Wenbin Zhang, Yinpng Pan, Jian Ding, Dexin Xu, Wenhai Wang
{"title":"Thrombolytic therapy efficacy in cardiopulmonary resuscitation: Investigating the effect of recombinant tissue plasminogen activator (Review).","authors":"Wenbin Zhang, Yinpng Pan, Jian Ding, Dexin Xu, Wenhai Wang","doi":"10.3892/mmr.2025.13700","DOIUrl":"https://doi.org/10.3892/mmr.2025.13700","url":null,"abstract":"<p><p>Cardiopulmonary resuscitation (CPR) involves a series of urgent life‑saving actions performed to manually revive a person in cardiac arrest. In >70% of CPR cases, the primary health deterioration is due to acute myocardial infarction or a significant pulmonary embolism. Fibrinolytic therapy, also known as thrombolytic treatment, functions by dissolving dangerous intravascular clots to mitigate ischemic damage through improved blood circulation. Thrombolytic therapy has emerged as a promising intervention within the context of CPR for patients experiencing cardiac arrest. Recombinant tissue plasminogen activator (rtPA) is a bioengineered version of a natural enzyme that converts plasminogen into plasmin, an enzyme essential for clot dissolution. The efficacy of rtPA in thrombolytic therapy for CPR suggests its potential as a valuable clinical tool. The present comprehensive review assessed the efficacy and safety of rtPA administration during CPR, focusing on outcomes such as survival rates, neurological function and potential complications. It also explored the underlying mechanisms by which rtPA may enhance reperfusion and improve patient outcomes, including clot dissolution, myocardial salvage and microcirculatory improvements. Additionally, it examined the optimal timing and dosing strategies for rtPA administration and its integration with standard CPR protocols and other supportive therapies.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation of nuclear transport of the transcriptional factor REST improves axon regeneration in peripheral nerves. 调控REST转录因子的核转运促进周围神经轴突再生。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI: 10.3892/mmr.2025.13703
Takamaru Suzuki, Kiyohito Naito, Daisuke Kubota, Yuji Ueno, Takako Negishi-Koga, Yasuhiro Yamamoto, So Kawakita, Norizumi Imazu, Kenjiro Kawamura, Nobutaka Hattori, Muneaki Ishijima
{"title":"Regulation of nuclear transport of the transcriptional factor REST improves axon regeneration in peripheral nerves.","authors":"Takamaru Suzuki, Kiyohito Naito, Daisuke Kubota, Yuji Ueno, Takako Negishi-Koga, Yasuhiro Yamamoto, So Kawakita, Norizumi Imazu, Kenjiro Kawamura, Nobutaka Hattori, Muneaki Ishijima","doi":"10.3892/mmr.2025.13703","DOIUrl":"https://doi.org/10.3892/mmr.2025.13703","url":null,"abstract":"<p><p>The expression of repressor element 1 silencing transcription factor (REST) in peripheral nerves increases with age, which leads to a decline in axon regeneration. However, the detailed mechanisms behind the decline in axon regeneration with age remain to be elucidated. The present study investigated the mechanism of nuclear transport of REST using hydrogen (H<sub>2</sub>), which has neuroprotective effects. First, aged mice as an animal model and REST‑overexpressed (REST‑OE) cells generated from the NIH‑3T3 fibroblast cell line as a cellular model were treated with H<sub>2</sub> to examine REST expression and growth‑associated protein 43 (GAP43) as an axon regeneration marker. Subsequently, to examine differences in the localization of REST expression, <i>in vitro</i> cell lines were fractionated into cytoplasmic and nuclear fractions for immunofluorescence staining and western blotting, and REST expression was quantified. Furthermore, to investigate the mechanisms of nuclear transport, REST nuclear transport proteins (REST‑interacting LIM domain protein, Huntingtin and dynactin subunit 1/p150<sup>Glued</sup>) and the autophagy‑related protein LC3 were semi‑quantified by western blotting. REST expression was decreased, and GAP43 expression was increased following H<sub>2</sub> administration in animal models and REST‑OE cells. REST intracellular localization analysis revealed that REST expression was significantly increased in the cytoplasm and significantly decreased in the nucleus in the REST‑OE + H<sub>2</sub> group compared with the REST‑OE group. Furthermore, the present findings revealed that the addition of H<sub>2</sub> resulted in a significant decrease in several REST nuclear transport proteins, subsequently suppressing the nuclear translocation of REST. These findings suggest that regulation of the nuclear transport of REST by H<sub>2</sub> improves the decline in axon regeneration.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Corrigendum] Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate‑induced osteoarthritis rats. [更正]桑叶水提物减轻碘乙酸钠诱导的骨关节炎大鼠关节软骨损伤和炎症反应。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-09-19 DOI: 10.3892/mmr.2025.13685
Jin-Woo Jeong, Hye Hyeon Lee, Jongsik Kim, Eun-Ok Choi, Hyun Hwang-Bo, Hong Jae Kim, Min Young Kim, Kyu Im Ahn, Gi-Young Kim, Ki Won Lee, Ki Young Kim, Sung Goo Kim, Su Hyun Hong, Cheol Park, Hee-Jae Cha, Yung Hyun Choi
{"title":"[Corrigendum] Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate‑induced osteoarthritis rats.","authors":"Jin-Woo Jeong, Hye Hyeon Lee, Jongsik Kim, Eun-Ok Choi, Hyun Hwang-Bo, Hong Jae Kim, Min Young Kim, Kyu Im Ahn, Gi-Young Kim, Ki Won Lee, Ki Young Kim, Sung Goo Kim, Su Hyun Hong, Cheol Park, Hee-Jae Cha, Yung Hyun Choi","doi":"10.3892/mmr.2025.13685","DOIUrl":"10.3892/mmr.2025.13685","url":null,"abstract":"<p><p>Subsequently to the publication of the above paper, an interested reader drew to the authors' attention that, concerning the histological data shown in Fig. 2A on p. 3844, the \"Control / Toluidin Blue\" data panel contained a section of data that was strikingly similar to data that had appeared previously in Fig. 2 in another article written by some of the same authors in <i>EXCLI Journal</i>. In addition, the \"MF / CTX‑II\" data panel was similarly strikingly similar to another data panel in Fig. 2 of the same article appearing in <i>EXCLI Journal</i>. Finally, it was also noted that the \"MF\" panel in Fig. 3 contained an overlapping section with the \"MIA+MF / COX‑2\" data panel in Fig. 6. After having re‑examined their original data, the authors realize that Figs. 2, 3 and 4 of the above paper had contained errors in terms of their assembly. The revised versions of Figs. 2, 3 and 4, now showing different histological images derived from two of the parallel experiments with a different magnification (scaled to 100 μm; the original images were scaled to 200 μm), are shown on the next two pages. Note that these errors did not adversely affect either the results or the overall conclusions reported in this study. All the authors agree with the publication of this corrigendum, and are grateful to the Editor of <i>Molecular Medicine Reports</i> for allowing them the opportunity to publish this. They also wish to apologize to the readership of the Journal for any inconvenience caused. [Molecular Medicine Reports 16: 3841‑3848, 2017; DOI: 10.3892/mmr.2017.7075].</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145086570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application potential of induced pluripotent stem cells in the research and treatment of autoimmune diseases (Review). 诱导多能干细胞在自身免疫性疾病研究和治疗中的应用潜力(综述)。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI: 10.3892/mmr.2025.13698
Zizhen Ye, Hanwei Zhao, Xuanhu Ye
{"title":"Application potential of induced pluripotent stem cells in the research and treatment of autoimmune diseases (Review).","authors":"Zizhen Ye, Hanwei Zhao, Xuanhu Ye","doi":"10.3892/mmr.2025.13698","DOIUrl":"https://doi.org/10.3892/mmr.2025.13698","url":null,"abstract":"<p><p>Autoimmune diseases are a group of disorders caused by the immune system mistakenly attacking the body's own tissues, including type 1 diabetes, rheumatoid arthritis and multiple sclerosis (MS). These diseases are typically accompanied by chronic inflammation and tissue damage, which markedly impact the quality of life of patients. Induced pluripotent stem cells (iPSCs), owing to their unlimited proliferative capacity and pluripotency, demonstrate unique advantages in the field of regenerative medicine. iPSCs can be induced to differentiate into various functional cells in vitro providing potentially important tools for disease modeling, drug screening and cell therapy. For example, iPSCs can be directed to generate cardiomyocytes, dopaminergic neurons, hepatocyte‑like cells and pancreatic β‑cells, highlighting their broad potential for translational applications. For treating autoimmune diseases, iPSCs can be utilized for tissue repair, replacement therapy and the induction of cells with immunoregulatory functions. The present review summarizes the latest advancements in iPSC technology and its research in various autoimmune diseases, including MS, rheumatoid arthritis, systemic lupus erythematosus, type 1 diabetes and systemic sclerosis. The present study also discusses the main challenges in the application of iPSCs, aiming to provide a theoretical basis and practical guidance for developing novel therapeutic strategies.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
From tumor immunotherapy to myocardial injury: A mechanistic discussion of immune checkpoint inhibitor‑related myocarditis (Review). 从肿瘤免疫治疗到心肌损伤:免疫检查点抑制剂相关心肌炎的机制探讨(综述)
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-10-03 DOI: 10.3892/mmr.2025.13697
Leiyang Dai, Yong Duan, Qiuxia Xiong
{"title":"From tumor immunotherapy to myocardial injury: A mechanistic discussion of immune checkpoint inhibitor‑related myocarditis (Review).","authors":"Leiyang Dai, Yong Duan, Qiuxia Xiong","doi":"10.3892/mmr.2025.13697","DOIUrl":"https://doi.org/10.3892/mmr.2025.13697","url":null,"abstract":"<p><p>Immune checkpoint inhibitors (ICIs) have revolutionized cancer therapy, markedly improving the survival rate of numerous malignancies. However, the emergence of immune‑related adverse events, particularly ICI‑related myocarditis, poses substantial clinical challenges. Despite its relatively low incidence, ICI‑related myocarditis demands urgent attention due to high mortality rates and treatment‑related cardiovascular toxicity. The present review provides an in‑depth analysis of the immunopathological mechanisms of ICI‑related myocarditis, with a focus on the physiological role of immune checkpoints, the core mechanisms of immune tolerance failure following ICI blockade, the influence of genetics and the microenvironment, and the effects of myocardial electrophysiology and hormones. Attention is also given to the cellular and molecular mechanisms, particularly the roles of T cells and macrophages. The current review highlights the critical interactions between these factors in the initiation and progression of ICI‑related myocarditis. In addition, clinical manifestations, diagnostic criteria incorporating advanced imaging and biomarker profiling, and differential diagnosis considerations are summarized, underscoring the necessity of multidisciplinary collaboration for timely intervention. Mechanistic elucidation of ICI‑related myocarditis will enable preventive strategies, optimized early diagnostics and improved patient outcomes, and may ultimately reduce the clinical incidence of this disease.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin exerts therapeutic effects on colorectal cancer by inducing pyroptosis through caspase‑1 activation. 姜黄素通过激活caspase‑1诱导大肠癌焦亡,发挥治疗大肠癌的作用。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-09-26 DOI: 10.3892/mmr.2025.13692
Jiajie Zhu, Liangjun Yang, Zheng Fang, Jiabin Chen, Yeqian Wu, Haiyan Liu, Shan Liu, Baoying Fei
{"title":"Curcumin exerts therapeutic effects on colorectal cancer by inducing pyroptosis through caspase‑1 activation.","authors":"Jiajie Zhu, Liangjun Yang, Zheng Fang, Jiabin Chen, Yeqian Wu, Haiyan Liu, Shan Liu, Baoying Fei","doi":"10.3892/mmr.2025.13692","DOIUrl":"10.3892/mmr.2025.13692","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a substantial global health challenge, with current treatments often leading to relapse and metastasis. Curcumin, a natural compound derived from turmeric, has shown promise in cancer therapy; however, its mechanisms in CRC are not fully understood. The present study aimed to investigate the role of curcumin in inducing pyroptosis, a form of inflammatory cell death, through caspase‑1 activation in CRC cells. Human CRC cell lines (HCT‑116 and SW480) and normal colon epithelial cells (FHC) were treated with curcumin at varying concentrations. Cell viability, migration and invasion were assessed using Cell Counting Kit‑8, wound healing and Transwell assays, respectively. Pyroptosis was evaluated through lactate dehydrogenase (LDH) release, TUNEL staining and western blot analysis of pyroptosis‑related proteins (caspase‑1, gasdermin D, nucleotide‑binding oligomerization domain‑like receptor protein 3, IL‑1β and IL‑18). The role of caspase‑1 was further examined using the inhibitor VX‑765. Curcumin significantly reduced CRC cell viability, migration and invasion in a dose‑dependent manner. In addition, it induced pyroptosis, as evidenced by cell membrane swelling, increased LDH release and upregulation of pyroptosis‑related proteins. Inhibition of caspase‑1 with VX‑765 attenuated these effects, confirming the role of caspase‑1 in curcumin‑induced pyroptosis. In conclusion, curcumin may exert anti‑CRC effects by inducing caspase‑1‑mediated pyroptosis, highlighting its potential as a therapeutic agent. These findings suggest that curcumin could be integrated into current CRC treatment strategies, particularly in targeting pyroptosis to enhance tumor suppression.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145150094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heterogeneity and characteristic changes in cardiomyocytes and non‑cardiomyocytes following myocardial infarction: Insights from single‑cell sequencing analysis (Review). 心肌细胞和非心肌细胞在心肌梗死后的异质性和特征变化:来自单细胞测序分析的见解(综述)。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-12-01 Epub Date: 2025-09-12 DOI: 10.3892/mmr.2025.13680
Xinxin Bian, Haizhu Gao, Cuimei Guo, Nan Lin, Lijun Gan, Xueying Chen
{"title":"Heterogeneity and characteristic changes in cardiomyocytes and non‑cardiomyocytes following myocardial infarction: Insights from single‑cell sequencing analysis (Review).","authors":"Xinxin Bian, Haizhu Gao, Cuimei Guo, Nan Lin, Lijun Gan, Xueying Chen","doi":"10.3892/mmr.2025.13680","DOIUrl":"10.3892/mmr.2025.13680","url":null,"abstract":"<p><p>Myocardial infarction (MI) is an important pathological event in cardiovascular disease and the heterogeneous changes in cardiomyocytes and non‑cardiomyocytes that occur following its occurrence have a profound effect on cardiac repair and functional recovery. Single‑cell sequencing, a novel technology for the analysis of tissues at the single‑cell level, permits a comprehensive insight into the heterogeneity of different cell populations. The current review presented a summary of the application of single‑cell sequencing to detect the heterogeneity of cardiomyocytes and non‑cardiomyocytes following MI. It focused on the classification and changes of cell clusters and explored the mechanisms of post‑infarction cardiac regeneration and remodeling with the aim of providing a theoretical foundation for identifying potential targets to enhance the diagnosis and treatment of post‑infarction cardiac remodeling.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12452225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metformin alleviates lung ischemia‑reperfusion injury via the SIRT1 pathway following lung transplantation in diabetic rats. 二甲双胍通过SIRT1通路减轻糖尿病大鼠肺移植后肺缺血再灌注损伤。
IF 3.5 3区 医学
Molecular medicine reports Pub Date : 2025-11-01 Epub Date: 2025-08-14 DOI: 10.3892/mmr.2025.13652
Hong Wei, Tian-Hua Liu, Li-Juan Zhang, Wei Yan, Can Ma, Shi-Hua Lv, Xian-Zhang Zeng, Wen-Zhi Li
{"title":"Metformin alleviates lung ischemia‑reperfusion injury via the SIRT1 pathway following lung transplantation in diabetic rats.","authors":"Hong Wei, Tian-Hua Liu, Li-Juan Zhang, Wei Yan, Can Ma, Shi-Hua Lv, Xian-Zhang Zeng, Wen-Zhi Li","doi":"10.3892/mmr.2025.13652","DOIUrl":"10.3892/mmr.2025.13652","url":null,"abstract":"<p><p>Diabetes mellitus (DM) exacerbates lung ischemia‑reperfusion (IR) injury and leads to poor survival in lung transplantation recipients. Metformin protects a number of tissues from IR injury. The present study aimed to investigate the effect of metformin on diabetic lung IR injury and the potential mechanisms. Rats with type 2 DM were exposed to metformin with or without administration of EX527, an inhibitor of the silent information regulator 1 (SIRT1) pathway, following lung transplantation. Lung function, alveolar‑capillary permeability, inflammatory response, oxidative stress, cell apoptosis, mitochondrial function, mitochondrial biogenesis key proteins and the SIRT1 signaling pathway were assessed. The effect of metformin on diabetic lung IR injury was evaluated by ELISA, oxidative stress assays, immunofluorescence, flow cytometry, TUNEL assay and western blotting. The results demonstrated that DM was associated with a significant increase in the IR‑induced alveolar‑capillary permeability, inflammatory response, oxidative stress and cell apoptosis. Furthermore, DM was associated with a significant decrease in mitochondrial function and biogenesis, SIRT1 expression and lung function. Metformin treatment markedly attenuated diabetic lung IR injury by alleviating the inflammatory response, oxidative stress and cell apoptosis, preserving mitochondrial function, and promoting mitochondrial biogenesis. However, EX527 inhibited the protective effect of metformin. In conclusion, metformin alleviated the inflammatory response, oxidative stress and cell apoptosis, preserved mitochondrial function, and promoted mitochondrial biogenesis via the activation of the SIRT1 pathway in diabetic lung IR injury.</p>","PeriodicalId":18818,"journal":{"name":"Molecular medicine reports","volume":"32 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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