Molecular Genetics and Genomics最新文献

{"title":"Genomic and functional characterization of Rhizobium metallidurans NS41, a heavy-metal-resistant, non-legume-associated endophyte that reduces oxidative stress in metal-exposed maize.","authors":"Iryna Bodnaruk, Małgorzata Rudnicka, Zofia Piotrowska-Seget","doi":"10.1007/s00438-026-02482-4","DOIUrl":"10.1007/s00438-026-02482-4","url":null,"abstract":"<p><p>Heavy metal (HM) contamination threatens plant health and poses significant challenges to agriculture and ecosystem functioning. Plant-associated microorganisms from HM-contaminated environments play a vital role in enhancing host stress tolerance and are promising candidates for sustainable, microbe-based strategies to support plant fitness. Non-legume-associated rhizobia, however, remain poorly described in this context. In this study, we characterized Rhizobium metallidurans strain NS41, an endophyte of goldenrod (Solidago canadensis) growing at a highly metal-contaminated site. To our knowledge, this is the first report of this species associated with a non-leguminous host, thereby expanding current knowledge about R. metallidurans. Genome analysis revealed determinants of HM resistance, oxidative and osmotic stress mitigation, and metabolism of plant-derived compounds, but no canonical nodulation genes, indicating a facultative endophytic, rather than nodulation-dependent, lifestyle adapted to metalliferous environments. In vitro, NS41 demonstrated tolerance to high Zn, Cd, and As(III) concentrations (MIC 20 mM, 2.5 mM, 5 mM, respectively), produced indole-3-acetic acid and ammonia, and solubilized phosphates. In a maize pot experiment in HM-contaminated soil, seed inoculation with NS41 resulted in lower hydrogen peroxide and anthocyanin levels and higher chlorophyll content, without significantly altering biomass or Cd accumulation. This identifies NS41 as a stress-mitigating endophyte rather than a strong direct growth promoter under the tested conditions. Together, these genomic features and stress-mitigating phenotype support further investigation of NS41 as a plant-protective bioinoculant for improving plant performance on HM-contaminated soils.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148549715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of TRPV3 gene polymorphisms with wool production traits in Zhexi Angora rabbits.","authors":"Rongrong Li, Bohao Zhao, Ning Shen, Yan Liu, Yang Chen, Xinsheng Wu","doi":"10.1007/s00438-026-02486-0","DOIUrl":"10.1007/s00438-026-02486-0","url":null,"abstract":"<p><p>Hair yield is the main economic value of Zhexi Angora rabbit, and it is an important index to measure its productive traits. The TRPV3 gene has been found to be associated with wool yield. This study aims to investigate the correlation between TRPV3 and wool production traits in rabbits, with the goal of improving wool yield. In the current study, flow cytometry and CCK-8 assays were used to investigate the effect of the TRPV3 gene on the proliferation and apoptosis of dermal papilla cells (DPCs) in rabbit hair follicles (HFs). Results showed that the TRPV3 gene regulates the mRNA expression of genes involved in HF development (BMP4, SFRP2, TGF-β1, WNT5a, and STAT1), as detected by qRT-PCR analysis. The core promoter region of TRPV3 was identified through dual luciferase activity tests, and Sanger sequencing was used to detect polymorphisms in both the exonic and promoter regions of TRPV3. The analysis showed a single SNP in the exon region, which had no significant association with wool production. However, five SNPs were detected in the promoter region, with the g.48897415G> A site showing a significant association with wool production in Zhexi Angora rabbits. Moreover, rabbits carrying the AA genotype also showed higher transcriptional activity. The mutation at g.48897415G> A in the promoter region introduced seven extra transcription factors (TFs) (RFX4, RFX3, RFX2, ZNF45, RFX1, Zfp668, and ZNF655). In conclusion, the g.48897415G > A mutation in TRPV3 is significantly associated with wool yield, and the TRPV3 gene inhibits the proliferation of DPCs. This locus can be used as a molecular marker for wool traits in rabbits, allowing early selection and precise breeding of high-wool-yield strains to shorten the breeding cycle and improve efficiency to enhance both the yield and quality of rabbit wool.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148549723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequencing reveals genes under selection for olfactory transduction in highland sheep.","authors":"Anees Ahmad, Muhammad Ibrahim, Muhammad Yaqoob, Muhammad Fahim, Sher Hayat Khan, Israr Ud Din, Majid Khan, Sohail Ahmad","doi":"10.1007/s00438-026-02477-1","DOIUrl":"10.1007/s00438-026-02477-1","url":null,"abstract":"<p><p>Kutta is an indigenous sheep breed found in the mountainous region of Swat, Pakistan. In this study we evaluated the performance of Kutta sheep within their natural habitat and identified genomic regions associated with environmental adaptability using whole genome sequencing. Flocks are mostly managed under a transhumant production system with year-round breeding. Kutta sheep demonstrated higher lambing efficiency under the available resources. Although the body size of rams and ewes was comparable, significant variation was observed among different age groups. Whole genome sequencing identified 15.46 million variants relative to Oar_v1.0 reference genome assembly. Exonic variants accounted for 2.3% of all protein coding gene variants and affected 6347 genes. These genes were primarily enriched in pathways related to G protein-coupled receptor signaling (700 genes) and olfactory transduction (500 genes). Selective sweep analysis detected 1930 genes within the regions of high homozygosity across the genome. Based on homozygosity patterns, biological relevance, and colocalization with known QTLs, 11 candidate genes were identified as putative selective signature. Five of these genes (OR4C6L, OR1086L, OR5AK2L, QR5T2L, OR9Q2) belonged to the olfactory receptor family. These olfactory receptor genes may contribute to high-altitude adaptation in Kutta sheep by enhancing their ability to locate food resources, recognize mates, and detect predators in challenging mountainous environments. Further screening of the OR4C6L sequence across 12 indigenous sheep breeds revealed marked differences between highland and lowland populations. Collectively, these findings provide comprehensive genomic insights into high-altitude adaptation of sheep and highlight the potential role of olfactory perception in survival and environmental fitness.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13369732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of neutrophil extracellular traps-associated inflammatory genes for patients with idiopathic pulmonary fibrosis.","authors":"Changhui Lang, Jieling Wu, Nuoxin Wang","doi":"10.1007/s00438-026-02473-5","DOIUrl":"10.1007/s00438-026-02473-5","url":null,"abstract":"<p><p>Neutrophil extracellular traps (NETs) facilitate inflammation and epithelial-mesenchymal transition (EMT), promoting the progression of pulmonary fibrosis. Various machine learning methods were used to screen for prognostic genes. Based on prognostic genes, a risk model was constructed to assess their ability for prognosis prediction of idiopathic pulmonary fibrosis (IPF). Mendelian Randomization (MR) analysis evaluated causal associations between IPF and prognostic genes, while GSE122960 examined cell-type-specific expression. A bleomycin-induced pulmonary fibrosis mouse was established, collagen deposition was assessed by Masson and Picrosirius Red staining, the expression of prognostic genes were validated by Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) and Western blot (WB), and NETs-related protein ( Cith3 and MPO ) were evaluated by immunofluorescence. A comprehensive analysis resulted in the identification of 3 prognostic genes (MMP1, CXCR7, and TPST1), followed by the development of a NET-inflammation-associated prognostic model, which well predicted the prognosis of IPF. Moreover, exploratory MR analysis suggested possible positive associations between genetically predicted MMP1 and TPST1 expression and IPF risk. Single-cell analysis revealed the expression of MMP1 and TPST1 differed significantly in monocytes between IPF patients and controls. Furthermore, significant differences were identified in functional pathways and immune cell infiltration between risk groups. Masson and Picrosirius Red staining confirmed collagen deposition in the bleomycin-induced pulmonary fibrosis mouse model. RT-qPCR and WB showed higher expression of MMP1, CXCR7, and TPST1 in pulmonary fibrosis mice than in controls. Cith3 + MPO double immunofluorescence staining showed increased NETs-related signals in the model group than the control. These findings provide potential prognostic markers for IPF patients and may enhance comprehension of the disease's underlying inflammatory and fibrotic processes.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALPK3 regulates cardiomyocyte apoptosis through the Notch1 signaling pathway.","authors":"Yonghua Yuan, Chen Zheng, Jiajun Ye, Jiaqi Zhou, Yiwei Niu, Yuan Cao, Xuehua He, Yuejuan Xu, Sun Chen","doi":"10.1007/s00438-026-02475-3","DOIUrl":"10.1007/s00438-026-02475-3","url":null,"abstract":"<p><p>ALPK3, a gene associated with pediatric cardiomyopathy, plays a pivotal role in cardiac development, yet the regulatory mechanisms governing its involvement in cardiomyocyte apoptosis are still not well understood. Detect the expression changes of ALPK3 in embryonic and neonatal mouse heart tissues at various developmental stages (E11.5, E12.5, E14.5, and P3). Establish ALPK3 knockdown cell lines to evaluate phenotypic changes in cardiomyocyte apoptosis. At the cellular level, validate the mechanism by which ALPK3 regulates cardiomyocyte apoptosis through the Notch1 signaling pathway. Transcriptional analysis of ALPK3 in embryonic mouse hearts revealed a progressive increase in expression during development, peaking in the perinatal period. In vitro experiments using ALPK3-knockdown AC16 cardiomyocytes demonstrated a 50% reduction in ALPK3 mRNA and protein levels, accompanied by significant apoptosis induction. RNA sequencing indicates that Notch1 signaling is significantly inhibited. ALPK3 deficiency suppressed Notch1 signaling, as evidenced by reduced levels of Hes-1 and NICD proteins; however, treatment with the Notch1 ligand Jagged1 reversed these effects. Furthermore, ALPK3 knockdown elevated pro-apoptotic BAX and decreased anti-apoptotic BCL-2 expression, whereas Jagged1 restored their levels. The findings indicate that ALPK3 regulates cardiomyocyte apoptosis and viability via the Notch1 pathway, offering mechanistic insights into ALPK3-associated cardiomyopathy and highlighting potential therapeutic targets for heart failure.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148437357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A CLN8 biallelic missense variant causes epilepsy with severe treatment-resistant psychosis.","authors":"Rimsha Zulfiqar, Ambreen Kanwal, Maham Hameed, Amina Iftikhar, Go Hun Seo, Sadaf Naz","doi":"10.1007/s00438-026-02480-6","DOIUrl":"10.1007/s00438-026-02480-6","url":null,"abstract":"<p><p>Epilepsy and psychosis are dysfunctions of the nervous system that may occasionally co-occur. The current study was designed to investigate the causes of psychosis with or without epilepsy in Pakistani families. We identified two consanguineous families in which all affected members had treatment-resistant psychosis while three patients also had epilepsy. Every participant was examined by psychiatrists and a psychologist, while epilepsy was diagnosed by neurologists. The doctors confirmed the presence of severe psychosis with or without epilepsy in the patients and their absence in other participants. Exome sequencing identified a biallelic variant c.570G > T; p.Trp190Cys in CLN8 that segregated with the phenotype of epilepsy with psychosis in one family whereas the results for the other family were negative. CLN8 variant affects an amino acid which is conserved in diverse vertebrate orthologues. In-silico analysis indicated that substitution of tryptophan with cysteine resulted in the loss of an intramolecular interaction, which may affect protein folding. This study emphasizes that CLN8-related phenotype can include severe treatment-resistant psychosis and also provides a genotypic extension.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148352391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RUNX2 influences alternative splicing of genes associated with gastric cancer progression by regulating SF3B6 expression in HGC-27 cells.","authors":"Chao Han, Junjie Liu, Hao Shi, Chao Zhang, Qiang Zhao, Yingming Song","doi":"10.1007/s00438-026-02459-3","DOIUrl":"10.1007/s00438-026-02459-3","url":null,"abstract":"<p><p>Gastric cancer (GC) is a highly malignant tumor with significant morbidity and mortality rates globally. Recent studies have shown that RUNX2, a member of the RUNX family known for its role in osteoblast differentiation and bone morphogenesis, is associated with the pathogenesis and progression of GC, while the underlying mechanisms of the pathogenesis and progression of GC are largely unknown. In this study, we investigated the role of RUNX2 in GC progression by analyzing its effects on gene expression and alternative splicing (AS) in HGC-27 cells. We silenced RUNX2 by small interfering RNA (siRUNX2), and then analyzed the globally regulated transcriptome profile by sequencing method (RNA-seq) to identify the differentially expressed genes (DEGs) and alternative splicing (AS) genes. The downstream targets of RUNX2 were identified by performing CUT&Tag and sequencing experiment in HGC-27 cells. Using RNA-seq, we identified 314 DEGs and 1120 regulated AS events (RASEs) in HGC-27 cells upon RUNX2 knockdown. The DEGs were enriched in pathways related to cell cycle regulation and apoptosis, while the RASEs were predominantly involved in cell cycle processes. These findings suggest that RUNX2 not only modulates gene expression but also extensively influences AS, which is crucial for cellular processes such as proliferation and survival. Notably, we found that RUNX2 regulates the expression of the splicing factor SF3B6 by binding to its promoter region. Our results showed that SF3B6 expression was significantly decreased in siRUNX2 samples, and its downregulation was associated with altered AS profiles of genes involved in the cell cycle. Our findings demonstrate that RUNX2 modulates the transcriptome and AS profile in GC cells, with a particular focus on its regulation of SF3B6. This study highlights the multifaceted role of RUNX2 in GC progression and suggests that targeting the RUNX2-SF3B6 axis could be a promising therapeutic strategy for GC.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Transcriptional characterization of sepsis in a LPS porcine model.","authors":"Ryan Neill","doi":"10.1007/s00438-026-02481-5","DOIUrl":"https://doi.org/10.1007/s00438-026-02481-5","url":null,"abstract":"","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic landscape of the Brahmin population of Gujarat, India, using X-chromosome STR markers.","authors":"Ankita Arunrao Fulkar, Vrunda Dave, Kopal Kapoor, Vaishali Sharma, Abhishek Singh, Malay Ashvinkumar Shukla","doi":"10.1007/s00438-026-02479-z","DOIUrl":"10.1007/s00438-026-02479-z","url":null,"abstract":"<p><p>X-chromosome Short Tandem Repeats (X-STR) have been utilized in forensics and population genetics studies for more than two decades, particularly in cases involving complex kinship analysis, disaster victim identification, sexual assault, and incest. Nevertheless, the genetic landscape of India remains underexplored using X-STR markers. In the present study, blood samples of 420 unrelated participants (133 females and 287 males) were analyzed from the Brahmin community of Gujarat, India. For the amplification of X-STR loci, the Investigator^® Argus X-12 QS Kit was utilized. 186 unique alleles were identified, suggesting high genetic diversity of the Brahmin population. Locus DXS10135 (PIC = 0.930) was the most polymorphic, and locus DXS7423 was the least polymorphic (PIC = 0.553). On analyzing the haplotype diversity, Linkage Group-1 (LG1) was found to have the highest haplotype diversity (0.997). Notably, LG1 was also the most informative and polymorphic linkage group (PIC = 0.994), preceded by LG4 (PIC = 0.989), LG2 (PIC = 0.988), and LG3 (PIC = 0.983). Based on the allelic and haplotype frequencies, the combined estimates for PD_F, PD_M, MEC_Desmarais, MEC_Kishida, MEC_Kruger, and MEC_{Desmarais duo} were ≥ 0.999, demonstrating high discriminatory power of all the loci. Furthermore, inter-population phylogenetic analysis revealed high genetic affinity of Gujarat's Brahmins with other populations of the Indian subcontinent, encompassing Madhya Pradesh's Bhil tribe and Punjab's Jat Sikh. The Gujarat Brahmins also have a shorter genetic distance with other South Asian populations, including the Bangladeshi population. This research indicates that the studied X-STR markers are highly polymorphic, offering significant insights for individual identification in forensics.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of microbial profiles between hospital wastewater and river water.","authors":"Lazaros A Gagaletsios, Tsolaire Sourenian, Dimitrios Karpouzas, Ibrahim Bitar, Costas Papagiannitsis","doi":"10.1007/s00438-026-02478-0","DOIUrl":"10.1007/s00438-026-02478-0","url":null,"abstract":"<p><p>This study aimed to compare the microbial profiles between hospital wastewater and river water to assess the dissemination of clinical isolates into the environment. Two types of water samples were collected from sampling sites which were geographically close (wastewater from the University Hospital of Larissa and river water from the Pineios River). Gram-negative bacteria isolated from both sample types were identified using MALDI-TOF. Furthermore, the minimum inhibitory concentration (MIC) of antibiotics were evaluated. A total of 54 Gram-negative isolates, belonging to diverse species, were collected from wastewater sample and river sample. All isolates were classified as MDR, exhibiting resistance to at least one agent from more than three different antibiotic classes. Based on species identification and susceptibility profiles, 27 isolates (19 from wastewater and 8 from river-water) were selected to be further characterized by whole-genome sequencing (WGS). Analysis of WGS data, revealed the presence of different STs, even in isolates belonging to the same bacterial species. Additionally, WGS data showed that carbapenemase-encoding genes were identified in the majority of isolates. PlasmidFinder identified a huge variety of plasmid replicons among the isolates studied. In conclusion, both hospital wastewater and river water contained isolates carrying clinically relevant resistance determinants, such as carbapenemase-encoding genes. The presence of these pathogenic bacteria in the river poses a significant public health concern. Although we could not identify the origin of MDR bacteria in the river sample, these findings highlight the growing threat of antimicrobial resistance in the environment and underscore the urgent need for improved treatment methods and stricter surveillance to control its spread.</p>","PeriodicalId":18816,"journal":{"name":"Molecular Genetics and Genomics","volume":"301 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13315120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}